3D-Human Small Intestinal Tissue Model System to Screen Drug Induced Gastrointestinal Toxicity and Wound Healing

2016 ◽  
Author(s):  
Seyoum Ayehunie
Keyword(s):  
Wound Healing ◽  
Model System ◽  
Gastrointestinal Toxicity ◽  
Intestinal Tissue ◽  
Drug Induced ◽  
Tissue Model ◽  
Small Intestinal

Novel 3-D human small intestinal tissue model to assess drug permeation, inflammation, and wound healing

2014 ◽  
Vol 229 ◽  
pp. S144 ◽  
Author(s):  
Seyoum Ayehunie ◽  
Zacharey Stevens ◽  
Tim Landry ◽  
Mitchell Klausner ◽  
Patrik Hayden ◽  
...  
Keyword(s):  
Wound Healing ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Drug Permeation ◽  
Small Intestinal

3D-human small intestinal tissue model as an alternate to animal testing to predict drug toxicity, permeability, and metabolism

2017 ◽  
Vol 280 ◽  
pp. S271 ◽  
Author(s):  
Seyoum Ayehunie ◽  
Zachary Stevens ◽  
Timothy Landry ◽  
Alex Armento ◽  
Mitchell Klausner ◽  
...  
Keyword(s):  
Drug Toxicity ◽  
Animal Testing ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Small Intestinal

A new organotypic 3-D small intestinal tissue model reconstructed from primary human cells

2013 ◽  
Vol 221 ◽  
pp. S88 ◽  
Author(s):  
Seyoum Ayehunie ◽  
Zachary Stevens ◽  
Timothy Landry ◽  
Alexander Armento ◽  
Mitchell Klausner ◽  
...  
Keyword(s):  
Human Cells ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Small Intestinal ◽  
Primary Human Cells

scholarly journals Involvement of gliadin, a component of wheat gluten, in increased intestinal permeability leading to non-steroidal anti-inflammatory drug-induced small-intestinal damage

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0211436 ◽  
Author(s):  
Sunao Shimada ◽  
Tetsuya Tanigawa ◽  
Toshio Watanabe ◽  
Akinobu Nakata ◽  
Naoki Sugimura ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Wheat Gluten ◽  
Intestinal Damage ◽  
Drug Induced ◽  
Inflammatory Drug ◽  
Small Intestinal ◽  
Anti Inflammatory

scholarly journals Effects of Shiga Toxin Type 2 on a Bioengineered Three-Dimensional Model of Human Renal Tissue

2014 ◽  
Vol 83 (1) ◽  
pp. 28-38 ◽  
Author(s):  
Teresa M. DesRochers ◽  
Erica Palma Kimmerling ◽  
Dakshina M. Jandhyala ◽  
Wassim El-Jouni ◽  
Jing Zhou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Renal Failure ◽  
Shiga Toxin ◽  
Three Dimensional ◽  
Kidney Injury ◽  
Renal Tissue ◽  
Three Dimensional Model ◽  
Drug Induced ◽  
Tissue Model

Shiga toxins (Stx) are a family of cytotoxic proteins that can cause hemolytic-uremic syndrome (HUS), a thrombotic microangiopathy, following infections by Shiga toxin-producingEscherichia coli(STEC). Renal failure is a key feature of HUS and a major cause of childhood renal failure worldwide. There are currently no specific therapies for STEC-associated HUS, and the mechanism of Stx-induced renal injury is not well understood primarily due to a lack of fully representative animal models and an inability to monitor disease progression on a molecular or cellular level in humans at early stages. Three-dimensional (3D) tissue models have been shown to be morein vivo-like in their phenotype and physiology than 2D cultures for numerous disease models, including cancer and polycystic kidney disease. It is unknown whether exposure of a 3D renal tissue model to Stx will yield a morein vivo-like response than 2D cell culture. In this study, we characterized Stx2-mediated cytotoxicity in a bioengineered 3D human renal tissue model previously shown to be a predictor of drug-induced nephrotoxicity and compared its response to Stx2 exposure in 2D cell culture. Our results demonstrate that although many mechanistic aspects of cytotoxicity were similar between 3D and 2D, treatment of the 3D tissues with Stx resulted in an elevated secretion of the kidney injury marker 1 (Kim-1) and the cytokine interleukin-8 compared to the 2D cell cultures. This study represents the first application of 3D tissues for the study of Stx-mediated kidney injury.

scholarly journals It Cuts Both Ways: An Annelid Model System for the Study of Regeneration in the Laboratory and in the Classroom

2021 ◽  
Vol 9 ◽  
Author(s):  
Veronica G. Martinez Acosta ◽  
Fausto Arellano-Carbajal ◽  
Kathy Gillen ◽  
Kay A. Tweeten ◽  
Eduardo E. Zattara
Keyword(s):  
Wound Healing ◽  
Developmental Process ◽  
Recovery Of Function ◽  
Single Species ◽  
Model System ◽  
Regenerative Capacity ◽  
Closely Related Species ◽  
Complete Replacement ◽  
Successful Recovery ◽  
The Individual

The mechanisms supporting regeneration and successful recovery of function have fascinated scientists and the general public for quite some time, with the earliest description of regeneration occurring in the 8th century BC through the Greek mythological story of Prometheus. While most animals demonstrate the capacity for wound-healing, the ability to initiate a developmental process that leads to a partial or complete replacement of a lost structure varies widely among animal taxa. Variation also occurs within single species based on the nature and location of the wound and the developmental stage or age of the individual. Comparative studies of cellular and molecular changes that occur both during, and following, wound healing may point to conserved genomic pathways among animals of different regenerative capacity. Such insights could revolutionize studies within the field of regenerative medicine. In this review, we focus on several closely related species of Lumbriculus (Clitellata: Lumbriculidae), as we present a case for revisiting the use of an annelid model system for the study of regeneration. We hope that this review will provide a primer to Lumbriculus biology not only for regeneration researchers but also for STEM teachers and their students.

Wound healing in a fetal, adult, and scar tissue model: A comparative study

2010 ◽  
Vol 18 (3) ◽  
pp. 291-301 ◽  
Author(s):  
Neeltje A. Coolen ◽  
Kelly C. W. M. Schouten ◽  
Bouke K. H. L. Boekema ◽  
Esther Middelkoop ◽  
Magda M. W. Ulrich
Keyword(s):  
Wound Healing ◽  
Comparative Study ◽  
Scar Tissue ◽  
Tissue Model
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