Novel 3-D human small intestinal tissue model to assess drug permeation, inflammation, and wound healing

2014 ◽  
Vol 229 ◽  
pp. S144 ◽  
Author(s):  
Seyoum Ayehunie ◽  
Zacharey Stevens ◽  
Tim Landry ◽  
Mitchell Klausner ◽  
Patrik Hayden ◽  
...  
Keyword(s):  
Wound Healing ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Drug Permeation ◽  
Small Intestinal

3D-human small intestinal tissue model as an alternate to animal testing to predict drug toxicity, permeability, and metabolism

2017 ◽  
Vol 280 ◽  
pp. S271 ◽  
Author(s):  
Seyoum Ayehunie ◽  
Zachary Stevens ◽  
Timothy Landry ◽  
Alex Armento ◽  
Mitchell Klausner ◽  
...  
Keyword(s):  
Drug Toxicity ◽  
Animal Testing ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Small Intestinal

A new organotypic 3-D small intestinal tissue model reconstructed from primary human cells

2013 ◽  
Vol 221 ◽  
pp. S88 ◽  
Author(s):  
Seyoum Ayehunie ◽  
Zachary Stevens ◽  
Timothy Landry ◽  
Alexander Armento ◽  
Mitchell Klausner ◽  
...  
Keyword(s):  
Human Cells ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Small Intestinal ◽  
Primary Human Cells

Wound healing in a fetal, adult, and scar tissue model: A comparative study

2010 ◽  
Vol 18 (3) ◽  
pp. 291-301 ◽  
Author(s):  
Neeltje A. Coolen ◽  
Kelly C. W. M. Schouten ◽  
Bouke K. H. L. Boekema ◽  
Esther Middelkoop ◽  
Magda M. W. Ulrich
Keyword(s):  
Wound Healing ◽  
Comparative Study ◽  
Scar Tissue ◽  
Tissue Model

scholarly journals A three-dimensional intestinal tissue model reveals factors and small regulatory RNAs important for colonization with Campylobacter jejuni

2020 ◽  
Vol 16 (2) ◽  
pp. e1008304 ◽  
Author(s):  
Mona Alzheimer ◽  
Sarah L. Svensson ◽  
Fabian König ◽  
Matthias Schweinlin ◽  
Marco Metzger ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Three Dimensional ◽  
Intestinal Tissue ◽  
Tissue Model ◽  
Small Regulatory Rnas ◽  
Regulatory Rnas

Composite sponges from sheep decellularized small intestinal submucosa for treatment of diabetic wounds

2020 ◽  
pp. 088532822096389
Author(s):  
Gamze Kara Magden ◽  
Cigdem Vural ◽  
Busra Yaprak Bayrak ◽  
Candan Yilmaz Ozdogan ◽  
Halime Kenar
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Rat Model ◽  
Small Intestinal Submucosa ◽  
Diabetic Rat ◽  
Control Group ◽  
Significant Difference ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
Diabetic Rat Model

Despite the fast development of technology in the world, diabetic foot wounds cause deaths and massive economical losses. Diabetes comes first among the reasons of non traumatic foot amputations. To reduce the healing time of these fast progressing wounds, effective wound dressings are in high demand. In our study, sheep small intestinal submucosa (SIS) based biocompatible sponges were prepared after SIS decellularization and their wound healing potential was investigated on full thickness skin defects in a diabetic rat model. The decellularized SIS membranes had no cytotoxic effects on human fibroblasts and supported capillary formation by HUVECs in a fibroblast-HUVEC co-culture. Glutaraldehyde crosslinked sponges of three different compositions were prepared to test in a diabetic rat model: gelatin (GS), gelatin: hyaluronic acid (GS:HA) and gelatin: hyaluronic acid: SIS (GS:HA:SIS). The GS:HA:SIS sponges underwent a 24.8 ± 5.4% weight loss in a 7-day in vitro erosion test. All sponges had a similar Young’s modulus under compression but GS:HA:SIS had the highest (5.00 ± 0.04 kPa). Statistical analyses of histopathological results of a 12-day in vivo experiment revealed no significant difference among the control, GS, GS:HA, and GS:HA:SIS transplanted groups in terms of granulation tissue thickness, collagen deposition, capillary vessel formation, and foreign body reaction (P > 0.05). On the other hand, in the GS:HA:SIS transplanted group 80% of the animals had a complete epidermal regeneration and this was significantly different than the control group (30%, P < 0.05). Preclinical studies revealed that the ECM of sheep small intestinal submucosa can be used as an effective biomaterial in diabetic wound healing.

scholarly journals In vitro enteroid-derived three-dimensional tissue model of human small intestinal epithelium with innate immune responses

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187880 ◽  
Author(s):  
Ying Chen ◽  
Wenda Zhou ◽  
Terrence Roh ◽  
Mary K. Estes ◽  
David L. Kaplan
Keyword(s):  
Immune Responses ◽  
Intestinal Epithelium ◽  
Three Dimensional ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Tissue Model ◽  
Small Intestinal Epithelium ◽  
Small Intestinal

scholarly journals Dynamic photopolymerization produces complex microstructures on hydrogels in a moldless approach to generate a 3D intestinal tissue model

2019 ◽  
Vol 11 (2) ◽  
pp. 025007 ◽  
Author(s):  
Albert G Castaño ◽  
María García-Díaz ◽  
Núria Torras ◽  
Gizem Altay ◽  
Jordi Comelles ◽  
...  
Keyword(s):  
Intestinal Tissue ◽  
Tissue Model
Sign in / Sign up

Export Citation Format

Share Document