FAECAL AMINO ACID PROFILES AS NOVEL NON-INVASIVE BIOMARKERS FOR THE DETECTION OF PAEDIATRIC INFLAMMATORY BOWEL DISEASE: A METABOLOMIC APROACH.

2017 ◽  
Author(s):  
Sofie Bosch
Keyword(s):  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Non Invasive ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Plasma amino acid profiles in dogs with inflammatory bowel disease

2019 ◽  
Vol 33 (4) ◽  
pp. 1602-1607 ◽  
Author(s):  
Yu Tamura ◽  
Hiroshi Ohta ◽  
Yumiko Kagawa ◽  
Tatsuyuki Osuga ◽  
Keitaro Morishita ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Plasma Amino Acid ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Fecal Amino Acid Analysis in Newly Diagnosed Pediatric Inflammatory Bowel Disease: A Multicenter Case-Control Study

2021 ◽  
Author(s):  
Jasmijn Z Jagt ◽  
Eduard A Struys ◽  
Ibrahim Ayada ◽  
Abdellatif Bakkali ◽  
Erwin E W Jansen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Newly Diagnosed ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel ◽  
Control Study ◽  
Pediatric Ibd

Abstract Background Fecal metabolomic profiles differ between pediatric inflammatory bowel disease (IBD) patients and controls and may provide new insights in the pathophysiology of IBD. The role of amino acids, however, is not fully elucidated. We aimed to assess fecal amino acid profiles in pediatric IBD. Methods In this case-control study, treatment-naïve, newly diagnosed pediatric IBD patients and a non-IBD control group, matched based on sex and age, were included in 2 tertiary centres. Fecal amino acid profiles were assessed using a targeted high-performance liquid chromatography technique. A random forest classifier method was used to develop a prediction model differentiating IBD from controls and predicting IBD phenotype. The association between IBD localization and amino acid concentrations was tested with ordinal regression models. Results We included 78 newly diagnosed IBD patients (40 Crohn’s disease [CD], 38 ulcerative colitis [UC]) and 105 controls. Patients with IBD could be differentiated from controls with an accuracy of 82% (sensitivity 63%, specificity 97%). Twenty-nine out of the 42 measured unique amino acids were included in the prediction model. Increased levels of tryptophan, taurine, alanine, ornithine, valine, histidine, and leucine were the most differentiating features. Children with CD and UC could be differentiated from the controls with an accuracy of 80% and 90%, respectively. Inflammatory bowel disease phenotype could not be predicted. Tryptophan, valine, and histidine levels were positively associated with more extended disease in UC patients (P < .05). Conclusions Fecal amino acids may enhance understanding of the role of host-microbial interactions in the pathophysiology of IBD and may evolve into biomarkers for pediatric IBD diagnostic and personalized medicine.

scholarly journals Fecal Amino Acid Profiles Exceed Accuracy of Serum Amino Acids in Diagnosing Pediatric Inflammatory Bowel Disease

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Sofie Bosch ◽  
Sofia El Manouni el Hassani ◽  
Marina Brizzio Brentar ◽  
Ibrahim Ayada ◽  
Abdellatif Bakkali ◽  
...  
Keyword(s):  
Amino Acids ◽  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Pediatric Inflammatory Bowel Disease ◽  
Serum Amino Acids ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Novel, Objective, Multivariate Biomarkers Composed of Plasma Amino Acid Profiles for the Diagnosis and Assessment of Inflammatory Bowel Disease

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e31131 ◽  
Author(s):  
Tadakazu Hisamatsu ◽  
Susumu Okamoto ◽  
Masaki Hashimoto ◽  
Takahiko Muramatsu ◽  
Ayatoshi Andou ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Amino Acid ◽  
Bowel Disease ◽  
Plasma Amino Acid ◽  
Amino Acid Profiles ◽  
Inflammatory Bowel

scholarly journals Fecal Calprotectin in Combination With Standard Blood Tests in the Diagnosis of Inflammatory Bowel Disease in Children

2021 ◽  
Vol 8 ◽  
Author(s):  
Shaun S. C. Ho ◽  
Michael Ross ◽  
Jacqueline I. Keenan ◽  
Andrew S. Day
Keyword(s):  
Inflammatory Bowel Disease ◽  
Platelet Count ◽  
Bowel Disease ◽  
Predictive Value ◽  
Screening Test ◽  
Fecal Calprotectin ◽  
Non Invasive ◽  
Blood Tests ◽  
Inflammatory Bowel ◽  
Sensitivity Specificity

Introduction: Fecal calprotectin (FC) is a useful non-invasive screening test but elevated levels are not specific to inflammatory bowel disease (IBD). The study aimed to evaluate the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of FC alone or FC in combination with other standard blood tests in the diagnosis of IBD.Methods: Children aged <17 years who had FC (normal range <50 μg/g) measured and underwent endoscopy over 33 months in Christchurch, New Zealand were identified retrospectively (consecutive sampling). Medical records were reviewed for patient final diagnoses.Results: One hundred and two children were included; mean age was 12.3 years and 53 were male. Fifty-eight (57%) of the 102 children were diagnosed with IBD: 49 with Crohn's disease, eight with ulcerative colitis and one with IBD-unclassified. FC of 50 μg/g threshold provided a sensitivity of 96.6% [95% confident interval (CI) 88.3–99.4%] and PPV of 72.7% (95% CI 61.9–81.4%) in diagnosing IBD. Two children with IBD however were found to have FC <50 μg/g. Sensitivity in diagnosing IBD was further improved to 98.3% (95% CI 90.7–99.1%) when including FC >50 μg/g or elevated platelet count. Furthermore, PPVs in diagnosing IBD improved when FC at various thresholds was combined with either low albumin or high platelet count.Conclusion: Although FC alone is a useful screening test for IBD, a normal FC alone does not exclude IBD. Extending FC to include albumin or platelet count may improve sensitivity, specificity, PPV and NPV in diagnosing IBD. However, prospective studies are required to validate this conclusion.

scholarly journals Breath Analysis Using eNose and Ion Mobility Technology to Diagnose Inflammatory Bowel Disease—A Pilot Study

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 55 ◽  
Author(s):  
Tiele ◽  
Wicaksono ◽  
Kansara ◽  
Arasaradnam ◽  
Covington
Keyword(s):  
Inflammatory Bowel Disease ◽  
Pilot Study ◽  
Ion Mobility ◽  
Bowel Disease ◽  
Breath Analysis ◽  
Diagnostic Tools ◽  
Exhaled Breath ◽  
Faecal Calprotectin ◽  
Non Invasive ◽  
Inflammatory Bowel

Early diagnosis of inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), remains a clinical challenge with current tests being invasive and costly. The analysis of volatile organic compounds (VOCs) in exhaled breath and biomarkers in stool (faecal calprotectin (FCP)) show increasing potential as non-invasive diagnostic tools. The aim of this pilot study is to evaluate the efficacy of breath analysis and determine if FCP can be used as an additional non-invasive parameter to supplement breath results, for the diagnosis of IBD. Thirty-nine subjects were recruited (14 CD, 16 UC, 9 controls). Breath samples were analysed using an in-house built electronic nose (Wolf eNose) and commercial gas chromatograph–ion mobility spectrometer (G.A.S. BreathSpec GC-IMS). Both technologies could consistently separate IBD and controls [AUC ± 95%, sensitivity, specificity], eNose: [0.81, 0.67, 0.89]; GC-IMS: [0.93, 0.87, 0.89]. Furthermore, we could separate CD from UC, eNose: [0.88, 0.71, 0.88]; GC-IMS: [0.71, 0.86, 0.62]. Including FCP did not improve distinction between CD vs UC; eNose: [0.74, 1.00, 0.56], but rather, improved separation of CD vs controls and UC vs controls; eNose: [0.77, 0.55, 1.00] and [0.72, 0.89, 0.67] without FCP, [0.81, 0.73, 0.78] and [0.90, 1.00, 0.78] with FCP, respectively. These results confirm the utility of breath analysis to distinguish between IBD-related diagnostic groups. FCP does not add significant diagnostic value to breath analysis within this study.

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