A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

Cyphostemma adenocaule (Steud. ex A. Rich.) is one of the specie plant that belongs to the family vitacea. In this study, Trilinolein was isolated and characterized from the methanol root extract of the plant. Column chromatography over silica gel granules as the stationary phase and eluted with a mobile phase mixture of n-Hex-EtA; EtA-CHCL3 and CHCL3-MeOH with gradient increasing polarity, followed by a second column using saphadex-LH20 and 100% MeOH as stationary and mobile phase vehicle respectively. TLC was developed with EtA 15: CHCL3 8: MeOH 4: H2O 1 as solvent system; sprayed with 10% H2SO4 ,Vanillin-sulphuric acid, and/ or Polyethylene glycol PEG and heat for spot detection and confirmation of bioactive principles. Compound CA1 was obtained and purified with CHCL3 to give a yellow semi-solid compound (0.23g). The 1H-NMR spectra showed 9 different signals; a signal peak of a glycerol (-CH2OCOR-) moiety on the first α-C chain and on the third αʹ-C at 4.143-4.187ppm and 4.296-4.325ppm respectively, while that of a β glycerol (-CHCOR-) at 5.286ppm. Signals of an allylic methylene group at 2.023-2.035ppm, Olefenic hydrogen group at signal peak of 5.362ppm and a diallylic methylene group at signal 2.790ppm were also observed. In the 13C NMR spectra of compound CA1, 57 carbon atoms where observed, multiple signals overlapping at a range of 14.13-34.21ppm corresponding to the aliphatic CH3 (C18), CH2 (C2, C3, C4, C5, C6, C7, C15, C16, and C17) and allylic (C8, C14) carbon atoms. Signals at 127.90-130.24ppm were assigned to the olefienic C atoms (C9, C10, C12, and C13) while signal of 172.87ppm and 173.32ppm were assigned to the carbonyl (C=O) carbon atoms (C1 and C2) respectively (Table 2). Analysis with DEPT-135, H-H COSY, HMBC and HSQC assignments of CA1 augments assignment of signals made for CA1 from 1H-NMR and 13C-NMR and corresponded to that of Trilinolein (<a href="https://pubchem.ncbi.nlm.nih.gov/#query=C57H98O6">C57H98O6</a>, MW 879.4 g/mol). The isolated compound was positive for the acrolein test for triglycerides; fat & oil and had an IC50 of 46.08µg/ml radical scavenging activity.

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A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm

10.26434/chemrxiv.13296539 ◽

2020 ◽

Author(s):

Abdulbasit Haliru Yakubu ◽

Iliya Ibrahim ◽

Abdulqadir bukar bababe ◽

Hassan Yesufu ◽

mohammed Garba Tom

Keyword(s):

Mobile Phase ◽

Nmr Spectra ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Solvent System ◽

Root Extract ◽

H Nmr ◽

Signal Peak ◽

Methylene Group ◽

C Nmr

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Antioxidant Metabolites from Limonium brasiliense (Boiss.) Kuntze

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-7-804 ◽

2004 ◽

Vol 59 (7-8) ◽

pp. 477-480 ◽

Cited By ~ 32

Author(s):

Ana P. Murray ◽

Silvana Rodriguez ◽

María A. Frontera ◽

María A. Tomas ◽

María C. Mulet

Keyword(s):

Chemical Constituents ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Spectroscopic Methods ◽

H Nmr ◽

Antioxidant Metabolites ◽

First Time ◽

C Nmr

Abstract A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-α-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the first time for this species. The characterization of these compounds was achieved by spectroscopic methods (1H NMR, 13C NMR and UV).

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A New Hepatoprotective Flavone Glycoside from the Flowers of Onopordum alexandrinum Growing in Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2011-5-608 ◽

2011 ◽

Vol 66 (5-6) ◽

pp. 251-259 ◽

Cited By ~ 2

Author(s):

Maha M. Salama ◽

Shahira M. Ezzat ◽

Amany A. Sleem

Keyword(s):

Free Radical ◽

Cell Damage ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Hepatoprotective Activity ◽

Free Radical Scavenging ◽

Flavone Glycoside ◽

H Nmr ◽

C Nmr

A bioactivity-guided fractionation of the ethyl acetate fraction of the flowers of Onopordum alexandrinum L. (Asteraceae) yielded a new flavonoidal glycoside designated as acacetin-7-O-galacturonide (9), alongside with nine known flavonoids; 6-methoxy-apigenin (hispidulin) (1), acacetin (2), apigenin (3), luteolin (4), kaempferol (5), eriodictyol (6), apigenin- 7-O-glucoside (7), luteolin-7-O-glucoside (8), and kaempferol-3-O-rutinoside (10). The compounds were assayed for their hepatoprotective activity against CCl4-induced hepatic cell damage in rats and free radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Compounds 4, 6, 9, and 10 have not been previously reported from flowers of O. alexandrinum L., and this is the first report of acacetin-7-O-galacturonide (9) in nature which has also shown significant hepatoprotective and free radical scavenging effects. The isolated compounds were identified using different spectroscopic methods (UV, 1H NMR, 13C NMR, HMQC, HMBC, and COSY).

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Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

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Conformational structure of ethylene glycol dibenzoate. Vibrational and NMR spectra

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19811913 ◽

1981 ◽

Vol 46 (8) ◽

pp. 1913-1929 ◽

Cited By ~ 3

Author(s):

Bohdan Schneider ◽

Pavel Sedláček ◽

Jan Štokr ◽

Danica Doskočilová ◽

Jan Lövy

Keyword(s):

Ethylene Glycol ◽

Vibrational Spectra ◽

Nmr Spectra ◽

Coupling Constants ◽

Conformational Structure ◽

Infrared And Raman Spectra ◽

H Nmr ◽

Liquid States ◽

Crystalline Forms ◽

C Nmr

It was found that three crystalline forms of ethylene glycol dibenzoate can be prepared. Infrared and Raman spectra of these three forms, as well as of the glassy and liquid states, were measured. From 3JHH coupling constants obtained by analysis of the 13C satellite band of the -CH2- group in 1H NMR spectra, and from the 3JCH coupling constants of the -CO.O.CH2- fragment obtained by analysis of the carbonyl band in 13C NMR spectra it was found that in the liquid state the -CH2-CH2- group exists predominantly in the gauche conformational structure, and the bonds C-O-C-C assume predominantly a trans orientation. The results of the analysis of NMR and vibrational spectra were used for the structural interpretation of conformationally sensitive bands in vibrational spectra of ethylene glycol dibenzoate.

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Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00242-2 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Vijayakumar Sundaram ◽

Selvaraju Sadhasivam ◽

Sivaraj Chandrasekaran ◽

Raaman Nanjian ◽

Arjun Pandian

Keyword(s):

Dermal Fibroblast ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Fibroblast Cell ◽

Phytochemical Analysis ◽

Root Extract ◽

Scavenging Activity ◽

Medicinal Uses

Abstract Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

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E-Homolupane Derivatives Substituted in Position 17 and 22a. 1H NMR, 13C NMR and IR Spectra

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19950619 ◽

1995 ◽

Vol 60 (4) ◽

pp. 619-635 ◽

Cited By ~ 1

Author(s):

Václav Křeček ◽

Stanislav Hilgard ◽

Miloš Buděšínský ◽

Alois Vystrčil

Keyword(s):

Nmr Spectra ◽

2D Nmr ◽

Coupling Constants ◽

Side Chain ◽

Oxidation Reactions ◽

H Nmr ◽

Complete Assignment ◽

Nmr Techniques ◽

Intramolecular Association ◽

C Nmr

A series of derivatives with various oxygen functionalities in positions 17,22a or 19,20 was prepared from diene I and olefin XVI by addition and oxidation reactions. The structure of the obtained compounds was confirmed by 1H NMR, 13C NMR and IR spectroscopy. The kind of intramolecular association of the 17α-hydroxy group was studied in connection with modification of the side chain and substitution in position 22a. Complete assignment of the hydrogen signals and most of the coupling constants was accomplished using a combination of 1D and 2D NMR techniques. The 1H and 13C NMR spectra are discussed.

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Antibacterial and Antioxidant Activities of Slender Amaranth Weed

Planta Daninha ◽

10.1590/s0100-83582020380100006 ◽

2020 ◽

Vol 38 ◽

Cited By ~ 1

Author(s):

M. AKBAR ◽

I.N. SHERAZI ◽

M.S. IQBAL ◽

T. KHALIL ◽

H.M. WAQAS

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Pseudomonas Syringae ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Bacterial Species ◽

Radical Scavenging ◽

Root Extract

ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.

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Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity

Scientific Reports ◽

10.1038/s41598-019-52166-w ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Farooq Azam ◽

Bashir Ahmad Chaudhry ◽

Hira Ijaz ◽

Muhammad Imran Qadir

Keyword(s):

Antioxidant Activity ◽

Structure Elucidation ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Dpph Radical ◽

Compound A ◽

Spectral Techniques ◽

H Nmr ◽

Aerial Parts

Abstract This study evaluates the antioxidant activity of Ranunculus muricatus and isolation and structure elucidation of the active constituents. The aerial parts of the plants were shade dried at room temperature and powdered and extracted with methanol. The free radical scavenging activity was evaluated by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. The percentage scavenging activity was determined based on the percentage of DPPH radical scavenged. Column chromatography was used in order to isolate the active compounds. Spectral techniques UV, IR, 1H NMR, 13CNMR and HREI-MS were used for the structure elucidation of the isolated compounds. Two isolated compounds, A (caffeoyl-β-d-glucopyranoside) and B (1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene), exibited a significant antioxidant activity as showed by DPPH radical scavenging method. Percentage inhibition for compound A (at 0.5 mM) was 82.67 ± 0.19 with IC50 of 93.25 ± 0.12 (μM), and for compound B (at 0.5 mM) was 69.23 ± 0.19 with IC50 of 183.34 ± 0.13 (μM). Quercetin was used as standard control. It was conclued from the present study that caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from methanol extract of aerial parts of Ranunculus muricatus posses antioxidant activity.

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Reactions of 3-(p-substituted-phenyl)-5-chloromethyl-1,2,4-oxadiazoles with KCN leading to acetonitriles and alkanes via a non-reductive decyanation pathway

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.14.280 ◽

2018 ◽

Vol 14 ◽

pp. 3011-3017

Author(s):

Akın Sağırlı ◽

Yaşar Dürüst

Keyword(s):

Nmr Spectra ◽

2D Nmr ◽

Synthetic Route ◽

X Ray ◽

H Nmr ◽

2D Nmr Spectra ◽

Tof Ms ◽

C Nmr

The present work describes an unfamiliar reaction of 5-(chloromethyl)-3-substituted-phenyl-1,2,4-oxadiazoles with KCN affording trisubstituted 1,2,4-oxadiazol-5-ylacetonitriles and their parent alkanes, namely, 1,2,3-trisubstituted-1,2,4-oxadiazol-5-ylpropanes. To the best of our knowledge, the current synthetic route leading to decyanated products will be the first in terms of a decyanation process which allows the transformation of trisubstituted acetonitriles into alkanes by the incorporation of KCN with the association of in situ-formed HCN and most likely through the extrusion of cyanogen which could not be detected or isolated. In addition, the plausible mechanisms were proposed for both transformations. The structures of the title compounds were identified by means of IR, 1H NMR, 13C NMR, 2D NMR spectra, TOF–MS and X-ray measurements.

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