Antibacterial and Antioxidant Activities of Slender Amaranth Weed

ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.

Download Full-text

Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/612805 ◽

2009 ◽

Vol 2009 ◽

pp. 1-6 ◽

Cited By ~ 29

Author(s):

K. Nagendra Prasad ◽

Jing Hao ◽

Chun Yi ◽

Dandan Zhang ◽

Shengxiang Qiu ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Liver Carcinoma ◽

Scavenging Activity ◽

Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

Download Full-text

Antibacterial and antioxidant activities of Agaricus bisporus (J.E. Lange) imbach from Pakistan

Bangladesh Journal of Botany ◽

10.3329/bjb.v48i4.49055 ◽

2019 ◽

Vol 48 (4) ◽

pp. 1075-1081

Author(s):

Hafiz Muhammad Waqas ◽

Muhammad Akbar ◽

Muhammad Sajjad Iqbal

Keyword(s):

Free Radical ◽

Ethyl Acetate ◽

Pseudomonas Syringae ◽

Agaricus Bisporus ◽

Pathogenic Bacteria ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Test Plant

In the present study, antibacterial and antioxidant (DPPH free radical scavenging activity) activity of an edible mushroom, Agaricus bisporus collected from Pakistan was investigated. In antibacterial assays, nhexane showed 4.83, 3.33, 16 and 3.33 mm inhibition zone diameter (IZD), chloroform showed 6.33, 16.17, 17 and 1.67 mm (IZD) while, ethyl acetate exhibited 30, 20, 19 and 28.67 mm (IZD) against four test plant pathogenic bacteria viz., Pseudomonas syringae Van Hall, Ralstonia solanacearum Yabuuchi et al. Xanthomonas axonopodis Dowson and Erwinia carotovora (Jones) Waldee, at 100 mg/ml concentration. Optical density (OD) was recorded at 517 nm. n-hexane extract showed 60.73, 47.6 and 33.05%, chloroform showed 89.97, 82.91 and 77.68% whereas, ethyl acetate exhibited 89.83, 65.82 and 23.59% free radical effects as compared to control. Gas chromatography mass spectrometry (GCMS) analysis showed the highest concentration of mono(2-ethylhexyl) phthalate.

Download Full-text

ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.10 ◽

2018 ◽

Vol 10 (1) ◽

pp. 44 ◽

Cited By ~ 1

Author(s):

Riza Shabrina ◽

Berna Elya ◽

Arikadia Noviani

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Group Identification ◽

Radical Scavenging ◽

Dpph Radical ◽

Frap Assay ◽

Antioxidant Power ◽

Dpph Radical Scavenging

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

Download Full-text

Antioxidant Activities of Yinchenhao (Artemisiae Capillaris) Leaves

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.18:386-391 ◽

2020 ◽

Vol 18 (4) ◽

pp. 386-391

Author(s):

Yonghun Kim ◽

Ang Li ◽

Junyu Wang ◽

Wancong Yu ◽

Fang Wan ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Therapeutic Effects ◽

Scavenging Activity

Yinchenhao (Artemisia capillaris Thunb.) is a pharmaceutical agent that not only shows therapeutic effects against hepatobiliary diseases but also offers various physiological benefits. This study examined the extraction rates of antioxidant extracts from Yinchenhao leaves using different solvents. The extraction rates using different solvents were as follows: ethanol (12.1 ± 0.87%) > water (7.7 ± 0.45%) > n-butanol (1.3 ± 0.16%) ethyl acetate > (1.3 ± 0.14%) > n-hexane (1.1 ± 0.15%). The n-butanol and ethyl acetate extracts showed higher 1,1-Diphenyl-2 picrylhydrazyl radical free radical scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging activity and ferric ion reducing antioxidant activity, while n-hexane extracts showed weak antioxidant activity. In conclusion, Yinchenhao leaf has potential as a natural antioxidant, and n-butanol and ethyl acetate may be effective extraction solvents for studying its antioxidant activities.

Download Full-text

Bagged Aronia Melanocarpa Tea: Phenolic Profileand Antioxidant Activity / Aronia Melanocarpa Filter Čaj: Fenolni Profil I Antioksidativna Aktivnost

Acta Facultatis Medicae Naissensis ◽

10.2478/afmnai-2014-0030 ◽

2014 ◽

Vol 31 (4) ◽

pp. 245-252 ◽

Cited By ~ 1

Author(s):

Jovana Veljković ◽

Jelena Brcanović ◽

Aleksandra Pavlović ◽

Snežana Mitić ◽

Biljana Kaličanin ◽

...

Keyword(s):

Antioxidant Activity ◽

Caffeic Acid ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Antioxidant Power ◽

Aronia Melanocarpa ◽

Tea Infusions

Summary While there is a large number of scientific papers reporting chemical composition and biological activities of Aronia melanocarpa, there is a lack information regarding the commercially available bagged tea. In order to supply new information on the antioxidant activity of the Aronia melanocarpa tea infusions, the aim of this study was to evaluate individual phenolic compounds which could be responsible for antioxidant activities of these beverages. Selected anthocyanins (cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin- 3-O-arabinoside, and cyanidin-3-O-xyloside), gallic acid, caffeic acid, rutin, morin, and protocatechuic acid were simultaneously detected from commercially available tea infusions using a High Performance Liquid Chromatographic (HPLC) method. The antioxidant activity was measured using five in vitro spectrophotometric methods: 1,1-diphenyl- 2-picrylhydrazyl radical scavenging activity (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid) radical cation scavenging activity (ABTS), ferric reducing-antioxidant power (FRAP) and reduction power (RP)Fe(III) to Fe(II). Obtained results showed that anthocyanins, predominantly of cyanidin-3-O-galactoside, are the major class of polyphenolic compounds in tea infusions. Among phenolic acids the most abundant is caffeic acid. A significant correlation between DPPH and ABTS and FRAP and RP suggested that antioxidant components in these beverages were capable scavenging free radicals and reducing oxidants. Generally, these beverages had relatively high antioxidant capacities and could be important dietary sources of antioxidant phenolics for the prevention of diseases caused by oxidative stress.

Download Full-text

HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts

Journal of Food Quality ◽

10.1155/2020/8851285 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Sung-Ho Lee ◽

Seong-Wook Seo ◽

Seung-Yub Song ◽

Deuk-Sil Oh ◽

Jung-Hyun Shim ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Ethanolic Extract ◽

Hot Water ◽

Total Phenolic ◽

Osmanthus Fragrans

A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.

Download Full-text

ANTIOXIDANT ACTIVITY TEST OF FRACTIONS FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s1.ff019 ◽

2020 ◽

pp. 97-100

Author(s):

ANNISA DHANIRA ◽

BERNA ELYA ◽

KATRIN BASAH

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dpph Radical ◽

Leaf Extracts ◽

Frap Assay ◽

Star Fruit

Objectives: Previous in vitro research showed that ethyl acetate fractions of star fruit leaves exhibit strong antioxidant activities toward 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC50 = 90 μg/ml). This study’s aim was to evaluate antioxidant activity of fractions from leaf extracts from star fruitsourced from three different regions in Indonesia (Depok, Sukabumi, and Subang) and, for the most active fraction (that with the highest antioxidantactivity), determine whether there was any correlation with phenolic and flavonoid content.Methods: Liquid–liquid partitioning, fractions were evaluated for in vitro antioxidant activity using DPPH radical scavenging and ferric reducingability of plasma (FRAP) assay.Results: The ethyl acetate fraction from the Subang region exhibited the strongest radical scavenging activity with both the DPPH assay (IC50 = 96 μg/ml)and the FRAP assay (FeEAC value = 1405 μmol/g).Conclusion: Ethyl acetate fractions from star fruit leaves could be used as natural antioxidants. No correlation was found between antioxidant activityand phenolic or flavonoid content.

Download Full-text

Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

Download Full-text

Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

Sugar Industry ◽

10.36961/si11177 ◽

2011 ◽

pp. 151-157 ◽

Cited By ~ 2

Author(s):

Marijana B. Saka ◽

Julianna F. Gyura ◽

Aleksandra Mišan ◽

Zita I. Šereš ◽

Biljana S. Pajin ◽

...

Keyword(s):

Antioxidant Activity ◽

Shelf Life ◽

Dietary Fiber ◽

Wheat Flour ◽

Room Temperature ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

Download Full-text

Biological Activities of Carlina Oxide Isolated from the Roots of Carthamus caeruleus

The Natural Products Journal ◽

10.2174/2210315509666190117152740 ◽

2020 ◽

Vol 10 (2) ◽

pp. 145-152 ◽

Cited By ~ 1

Author(s):

Imane Rihab Mami ◽

Rania Belabbes ◽

Mohammed El Amine Dib ◽

Boufeldja Tabti ◽

Jean Costa ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Skin Diseases ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Penicillium Expansum ◽

Natural Food ◽

Antioxidant Power

Background: Carthamus caeruleus belongs to the Asteraceae family. The roots are traditionally used as healing agents. They help to heal burns and treat skin diseases. They are also used against joint inflammation and are very effective against diseases such as irritable bowel syndrome for cancer patients. Objectives: The purpose of this work was i) to study the chemical composition of i) the essential oil and hydrosol extract of Carthamus caeruleus, ii) to isolate the major component of both extracts and iii) to evaluate their antioxidant, antifungal and insecticidal activities. Methods: The essential oil and hydrosol extract obtained from the roots were studied by GC and GC/MS. The antioxidant activities were performed using two different methods i) Radical scavenging activity (DPPH) and ii) the Ferric-Reducing Antioxidant Power (FRAP), using BHT as a positive control. Whereas, the antifungal activity of the essential oil and Carlina oxide was investigated against plant fungi. The fumigation toxicity of C. caeruleus essential oil besides Carlina oxide was evaluated against adults of Bactrocera oleae better known as the olive fly. Results: The essential oil and hydrosol extract were mainly represented by acetylenic compounds such as carline oxide and 13-methoxy carline oxide. Carlina oxide was isolated and identified by 1H and 13C NMR spectroscopic means. The results showed that Carlina oxide presented interesting antioxidant and antifungal properties, while C. caeruleus root essential oil had better insecticidal activity. Furthermore, Carlina oxide has demonstrated promising in vivo antifungal activity to control infection of apples by Penicillium expansum. Conclusion: Carlina oxide can be used as a natural food preservative and alternative to chemical fungicides to protect stored apple against Penicillium expansum.

Download Full-text