Comparison of in-vitro anti-inflammatory activity of various extracts of Solanum tuberosum

2020 ◽  
Vol 9 (2) ◽  
pp. 14-17
Author(s):  
Magesh M
Keyword(s):  
Solanum Tuberosum ◽  
Chemical Constituents ◽  
Cardiovascular Complications ◽  
The Body ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Synthetic Drugs ◽  
Anti Inflammatory ◽  
Staple Crop

Medicinal plants are vital and have been used in traditional systems of medicine of India. Their importance is not restricted to direct usage in the treatment. Still, also their chemical constituents were used as leads for many potential synthetic drugs that are drugs of choice to treat dangerous diseases. Inflammations are the significant sources of diseases and deteriorations in the body that result in body pains, and brain damages too. The inflammation is a body responding to the stimulus that causes harm to the body. Solanum tuberosum is a common staple crop available in the kitchen of every house and is used a food. It had a rich source of starch and anthocyanins. Even though it is used as a food crop, the pharmacological activities of potato had been investigated as IBD (Inflammatory Bowel Disease), various cancers, Hyperlipidemia and growing cardiovascular complications etc. the potato has been selected for the current study to compare the anti-inflammatory activity of various extracts of the plant. The action of the extracts was tested using two methods invivo using albino rats. The extracts showed a better movement, but when compared to the standard, they are less potent. But in consideration of the side effects and toxicity of standard synthetic drug the anti-inflammatory action can be compromised a bit to give a safe and potent activity

Pharmacognostical Standardization, Isolation of Phytoconstituents (β-sitosterol), HPTLC analysis of extracts of Operculina turpethum (Linn.) roots and evaluation of cytotoxic, in vitro & in vivo anti-inflammatory activities

2021 ◽  
Vol 18 ◽  
Author(s):  
Akash Ved ◽  
Shweta Gupta ◽  
Namrata Singh ◽  
Karuna S. Shukla ◽  
Om Prakash ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Inflammatory Activity ◽  
Control Group ◽  
Albino Rats ◽  
Cell Viability Assay ◽  
Edema Formation ◽  
Egg Albumin ◽  
Anti Inflammatory

Background: Operculina turpethum (Linn.) Silva Manso, family- convolvulaceae, is an important plant in Indian conventional system of medicine which is extensively employed by different tribes in many countries to cure edema and painful conditions like arthritis, back pain; hyperlipidemia, diabetes mellitus, liver disorders, skin disorders and to regulate bowel functions. Objective: The roots of O. turpethum (Linn.) was subjected to physicochemical, phytochemical standardization, the chromatographic separation which was accomplished by column chromatography, TLC, and HPTLC, further, the acute toxicity, cytotoxic and anti-inflammatory activities of Operculina turpethum roots were estimated by in vivo and in vitro models. Materials and Methods: This study includes percentage yield of extraction, organoleptic evaluation along with the analysis of its physicochemical investigations & preliminary phytochemical estimation. The isolation of active phytoconstituents was done by column chromatography, and the isolated compound was then exposed to TLC and HPTLC analysis. Cytotoxic activity was tested by WST-1 based cell viability assay on HepG2 cells. Anti-inflammatory activity of methanol extract (ME) was evaluated against inflammation occur by both in vitro and in vivo method. Results: The methanolic extract exhibited the presence of most of the phytoconstituents out of all the extracts, the phytoconstituent phytosterol, i.e., β-sitosterol was isolated by column chromatography, identified and quantified by TLC and HPTLC, which is liable for anti-inflammatory activity. The amount of β-sitosterol was estimated to be 14.09 µg in 10.00 mg fraction of MEOT. MEOT is devoid of toxicity up to 2000 mg/kg in Wistar albino rats. It was analysed that in vitro anti-inflammatory activity of MEOT by egg albumin denaturation method exhibited a incredible decrement in turbidity and increasing the percentage inhibition of albumin denaturation (60.52%) in MEOT treated group as compared with control group. In egg albumin-induced edema in rats, MEOT at the dose of 400 mg/kg reduced the edema formation (3.03 ± 0.02) induced by egg albumin at 4th h. In cotton pellet-induced granuloma in rats, MEOT at the dose of 400 mg/kg displayed maximum granuloma inhibition (51.06%) which is similar to that of indomethacin. Conclusion: From the obtained findings it is confirmed that O. turpethum contains β-sitosterol which is responsible for potent anti-inflammatory activity without causing cytotoxicity to the plant. The results suggested that ME of O. turpethum roots had high potential for application as an anti-inflammatory agent. The recognization and confirmation of the plant can be obtaineded from the study and will present data which is aidful in determining the quality and purity of a crude drug which further helps in preventing its adulteration.

scholarly journals In vitro and In vivo Models for Anti-inflammation: An Evaluative Review

2019 ◽  
pp. 3-15 ◽  
Author(s):  
Fabian Ifeanyi Eze ◽  
Philip F. Uzor ◽  
Peter Ikechukwu ◽  
Bonaventure C. Obi ◽  
Patience O. Osadebe
Keyword(s):  
Inflammatory Activity ◽  
Assay Method ◽  
In Vivo Models ◽  
Synthetic Drugs ◽  
Mediators Of Inflammation ◽  
In Vivo Animal Models ◽  
Anti Inflammatory ◽  
Anti Inflammation

Anti-inflammatory activity study involves developing a model that mimics, or provokes the production or release of, the biochemical mediators of inflammation, and monitoring the response of these biochemicals to the test drugs. This report constitutes an updated review of the in vitro and in vivo study models for assessing anti-inflammatory activity in plant extracts and synthetic drugs. The materials, instrumentation, and methods involved, as well as the mechanism of anti-inflammatory activity tested in each model, are extensively described. The merits and limitations of each method have also been discussed. A comparative assessment of the in vivo animal models vis-à-vis, the in vitro enzyme models have been made to assist scientists and researchers in the choice of assay method in terms of sensitivity, reliability, duration of test, ethical, and cost considerations.

scholarly journals Synthesis, characterization and biological evaluation of some novel N-Mannich bases of heterocyclic 1,3,4-thiadiazole.

2019 ◽  
Vol 9 (4-A) ◽  
pp. 220-228
Author(s):  
Piush Sharma ◽  
Charanjeet Singh
Keyword(s):  
Mannich Reaction ◽  
Mannich Bases ◽  
Biological Evaluation ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Plate Method ◽  
Standard Drug ◽  
Excellent Activity ◽  
Anti Inflammatory

A series of some novel N-Mannich bases of heterocyclic 1,3,4-thiadiazole were synthesized through the condensation reaction of 1,3,4-thiadiazole containing a aromatic secondary amine, aromatic aldehydes and cyclic compounds employing Mannich reaction and using conventional synthesis. All the synthesized compounds were obtained in the range of 57.41-83.3 % yield. The structures of synthesized compounds were confirmed by UV, IR, 1H NMR spectroscopy. the essential structural features responsible for interaction with receptor site are established within a suggested pharmacophore. The in vitro antibacterial activity of the synthesized compounds was determined, against two Gram-positive bacteria, viz. S. aureus & B. subtilis and Gram-negative, viz. E. coli and K. pneumoniae, by cup-plate method using the standard drug ciprofloxacin. Minimum inhibitory concentrations (MIC) changed in the range of 1.56_ _ 200 mg mL_1. Compound 3b exhibited excellent activity against both bacteria. The in vitro antifungal activity of the synthesized compound was also evaluated by cup-plate method against the fungi A. niger and C. albicans compared with the standard drug Fluconazole. Compound 4a, 8a exhibited excellent activity against both fungi. The result has shown that the compounds are quite active against pathogens under study and were nontoxic. The anti-inflammatory activity of the compound was evaluated, on albino rats, by carageenan induced rat paw oedema method using the standard drug diclofenac sodium. Compound 7b and 8c exhibited excellent anti-inflammatory and analgesic pharmacological activities. Structurally the compound 7b has a greater number of unsaturated hydrocarbons in schiff base, which shows good lipophilic properties within electron rich morpholine ring in Mannich base. Statistical significance of differences between group was determined by one-way analysis of variance (ANOVA). Among the synthesized compounds 3a, 4b, 5c, 7b, 8a and 8c were found be the most active. All the synthesized compounds were found to be low lethal as ascertained by LD50 test. Keywords: N- Mannich bases of heterocyclic 1,3,4-thiadiazole derivatives; Mannich reaction; antimicrobial agents; anti-inflammatory activity;

Anti-inflammatory Activity of Tuber Extracts of Solanum tuberosum in Male Albino Rats

2016 ◽  
Vol 39 (6) ◽  
pp. 421-425
Author(s):  
Mohd Fasih Ahmad ◽  
Syed Mahboob Ahmad ◽  
Raj K. Keservani ◽  
Anil K. Sharma
Keyword(s):  
Solanum Tuberosum ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Anti Inflammatory

scholarly journals Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

Molecules ◽  
2017 ◽  
Vol 22 (1) ◽  
pp. 125 ◽  
Author(s):  
Yu Jing ◽  
Yi-Fan Zhang ◽  
Ming-Ying Shang ◽  
Guang-Xue Liu ◽  
Yao-Li Li ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
Asarum Heterotropoides

scholarly journals Chemical Constituents of the Folk Medicinal Plant Argyreia acuta Lour. and Their Anti-Inflammatory Activity

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110445
Author(s):  
Yunqing Li ◽  
Xiaoyong Zhu ◽  
Liuyan Mo ◽  
Cailan Liu ◽  
Jun Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nuclear Magnetic Resonance ◽  
Medicinal Plant ◽  
Chemical Constituents ◽  
Inflammatory Activity ◽  
Two Dimensional ◽  
One Dimensional ◽  
Phytochemical Investigation ◽  
Anti Inflammatory

Phytochemical investigation of the folk medicinal plant Argyreia acuta Lour. has led to the isolation of a pair of new 3-alkylated coumarin enantiomers, (±)-acutamarin [(±)-1] and 3 known structurally related coumarins (2-4), along with 4 known flavonoids (5-8). The structures of these compounds were elucidated on the basis of extensive spectroscopic (including one-dimensional and two-dimensional nuclear magnetic resonance) analyses and by comparison of their spectral data with those reported in the literature. The absolute configurations of ( − )-1 and ( + )-1 were proposed by comparison of experimental and calculated electronic circular dichroism data. Compounds 5 and 7 exhibited in vitro anti-inflammatory activity by inhibiting the production of nitric oxide with IC50 values of 24.54 ± 0.36 μM and 10.60 ± 0.15 μM, respectively.

scholarly journals In-vitro Anti-Inflammatory activity of Digera muricata Extracts

2020 ◽  
Vol 11 (4) ◽  
pp. 5748-5751
Author(s):  
Balaji Subramaniyan R ◽  
Jaikumar S ◽  
Sekkizhar M
Keyword(s):  
In Vitro Models ◽  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Ethical Problems ◽  
Synthetic Drugs ◽  
Tissue Degeneration ◽  
Alternative Means ◽  
Anti Inflammatory ◽  
Work Done

Inflammation is a process that is generally termed for pain and redness, which includes swelling. But it is defined as a heterogeneous group of processes which result in the pain and increase in vascular circulation and denaturation of protein that results in tissue degeneration. There are many synthetic drugs available to treat and relieve the inflammation. They are potent and have many side effects too. So there is an increased search for alternative means of treatment option. Thereby there was a high focus on the medicinal plants and herbs. Digera muricate, which is used to treat many diseases like analgesic activity, antidiabetic activity and anticancer activity too, this plant has proven to possess the antioxidant potential also. So there is no enough work done to verify the inflammatory potential of the plant. This was the primary consideration for this research to select the plant to prove it has anti-inflammatory potential. Having in the account that the ethical problems in the usage of lab animals, the anti-inflammatory potential of the plant was evaluated in-vitro using enzyme inhibition assays. The in-vitro models of the inflammatory activity are used for testing the anti-inflammatory activity, and the results were not different from invivo models. The extracts showed a dose based rise in the action, and the plant process a better anti-inflammatory activity than the standard drugs.

scholarly journals In vitro and In vivo Anti-inflammatory Activity of the Extracts of Whole Plant Argyreia imbricata (Roth) Sant. & Patel

2020 ◽  
Vol 11 (4) ◽  
pp. 7317-7322
Author(s):  
Sebastin V ◽  
Gopalakrishnan G ◽  
Sreejith M ◽  
Anoob Kumar K I
Keyword(s):  
Ethyl Acetate ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Significant Activity ◽  
In Vivo Evaluation ◽  
Whole Plant ◽  
Anti Inflammatory

Plants of the genus Argyreia have ethnomedicinal importance, and several pharmacological activities are also reported. In this study, the anti-inflammatory activity of different extracts of Argyreia imbricata was evaluated by in vitro and in vivo methods. In both evaluations, standard, Diclofenac sodium was used for comparative evaluation. In this study, extraction of powdered whole plant material was done with different solvents viz., petroleum ether, chloroform, ethyl acetate and methanol by soxhelation. In vitro anti-inflammatory activity of all the prepared extracts was evaluated by stabilization of human red blood cell (HRBC) membrane in different temperature and tonicity conditions. Among the six different concentrations of four tested extracts, the ethyl acetate and methanol extracts (1000μg/ml) showed significant activity in the in vitro evaluation. They were selected for the in vivo evaluation on the paw oedema induced by carrageenan on Wistar albino rats. Two doses, 200mg.kg-1 and 400mg.kg-1 of the test extracts were subjected to evaluation. Both the tested extracts showed the activity, particularly, the methanol extract in the dose of 400mg.kg-1 showed significant activity. Results of this study strongly supported the anti-inflammatory activity of the tested extracts. Further, studies on toxicity, identification, isolation of the active constituents may give useful results.

scholarly journals Study of anti-inflammatory activity of Ficus racemosa linn stem bark extract in albino rats

2021 ◽  
Vol 10 (3) ◽  
pp. 231
Author(s):  
Priya Mohan B. N. ◽  
Suma Jayaram ◽  
Sanitha Kuriachan
Keyword(s):  
Side Effects ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Control Group ◽  
Albino Rats ◽  
Standard Drug ◽  
Paw Oedema ◽  
Cotton Pellet ◽  
Anti Inflammatory

Background: Inflammation is part of the complex biological response of vascular tissues to harmful stimuli. Though there are standard anti-inflammatory drugs like aspirin, diclofenac, etc., these are not free of side effects. This has led to an increase in demand for natural products with anti-inflammatory activity having less side effects. Hence the study was conducted to evaluate the anti-inflammatory activity of ethanolic extract of Ficus racemose (EEFR) in albino rats.Methods: Healthy albino rats of either sex were divided into 4 groups of 6 animals each. Group1-control, group 2-diclofenac sodium 2 mg/kg and group 3 and 4 EEFR (200 and 400 mg/kg respectively), anti-inflammatory activity was evaluated by Carrageenan induced paw oedema: formalin induced-peritonitis and cotton pellet induced granuloma model for in vivo activity and protein denaturation test for in vitro activity.Results: EEFR exhibited significant in vitro (p<0.001) anti-inflammatory effect at the dose of 200 and 400 mg/kg. EEFR produced 61.37% inhibition at the dose of 400 mg/kg and diclofenac (standard drug) produced 62.95% of inhibition after 3 hours of drug treatment in carrageenan induced paw oedema. The exudate volume was decreased in formalin induced peritonitis by EEFR and diclofenac significantly (p<0.001). In cotton pellet induced granuloma EEFR (400 mg/kg) and diclofenac showed decreased formation of granuloma by 28.36% and 28.00% (p<0.001) respectively.Conclusions: EEFR has significant anti-inflammatory activity in both acute and chronic model in a dose dependant manner in comparison with standard drug.

Comparative anti-inflammatory evaluation of five plants of Solanaceae members

2020 ◽  
Vol 5 (1) ◽  
pp. 12-15
Author(s):  
Hemalatha S ◽  
Shaheedha SM
Keyword(s):  
Human Body ◽  
Chemical Constituents ◽  
Chemical Constituent ◽  
Solanum Nigrum ◽  
The Body ◽  
The Other ◽  
Inflammatory Activity ◽  
Tissue Temperature ◽  
Surrounding Tissue ◽  
Anti Inflammatory

Inflammation is processed by which the body responds to the external stimulus that causes any harm or damage to the human body. Inflammation is good with some respect and if it grows and builds up in the body will lead to many other complications and manifests itself in the form of pain, redness, swelling and spike in surrounding tissue temperature. Solanaceae members are known to have rich chemical constituents and a variety of them being alkaloids. Whatever may be the chemical constituent, most of the Solanaceae members have been already investigated and proven for their anti-inflammatory activity in many available methods. In research, all five species of Solanum had been compared for their anti-inflammatory activity in two models to determine which has a better activity in comparison to the other. Overall it can be said that Solanum nigrum showed a better activity compared to other extracts and the activity was comparably significant and similar to that of the standard drugs dexamethasone and indomethacin too.

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