scholarly journals Tridimenzionalno dokumentiranje stečkov srednjeveške župe Dabar v Bosni in Hercegovini / Three-dimensional documentation of the tombstones of the medieval parish of Dabar in Bosnia and Herzegovina

2021 ◽  
Vol 9 (1) ◽  
pp. 109-123
Author(s):  
Špela Čaval ◽  
Maša Sakara ◽  
Tjaša Miklavčič
Keyword(s):  
Pilot Study ◽  
Material Culture ◽  
Bosnia And Herzegovina ◽  
Three Dimensional ◽  
3D Scanning ◽  
Archaeological Research ◽  
Western Balkans ◽  
Climate Influences

Stećci are medieval tombstones that can be found in situ in the landscapes of the Western Balkans. Only approx. 8% of stećci have various decorations in the form of low relief, while less than 400 tombstones also bear inscriptions. Climate influences often cause erosion of these engravings, which makes them increasingly difficult to detect and identify. As part of the archaeological research on the stećci phenomenon in the area of the medieval župa Dabar (modern municipalities of Berkovići and Bileća), a pilot study of 3D scanning of these monuments with a hand-held scanner was performed. Eight tombstones, two with an inscription and six with decorations, were thus scanned in their entirety, in order to evaluate the visibility and recognition of the engravings, and to assess the technique for detailed documentation of material culture itself. The article presents the process of scanning and analyzes the results.

scholarly journals Three-Dimensional Nuclear Telomere Profiling as a Biomarker for Recurrence in Oligodendrogliomas: A Pilot Study

2020 ◽  
Vol 21 (22) ◽  
pp. 8539
Author(s):  
Macoura Gadji ◽  
Shubha Mathur ◽  
Brigitte Bélanger ◽  
Jaganmohan Reddy Jangamreddy ◽  
Josée Lamoureux ◽  
...  
Keyword(s):  
Pilot Study ◽  
Fluorescent In Situ Hybridization ◽  
Three Dimensional ◽  
Telomere Dysfunction ◽  
Short Term ◽  
Time To Recurrence ◽  
Potential Link ◽  
Touch Preparation

Mechanisms of recurrence in oligodendrogliomas are poorly understood. Recurrence might be driven by telomere dysfunction-mediated genomic instability. In a pilot study, we investigated ten patients with oligodendrogliomas at the time of diagnosis (first surgery) and after recurrence (second surgery) using three-dimensional nuclear telomere analysis performed with quantitative software TeloView® (Telo Genomics Corp, Toronto, Ontario, Canada). 1p/19q deletion status of each patient was determined by fluorescent in situ hybridization on touch preparation slides. We found that a very specific 3D telomeric profile was associated with two pathways of recurrence in oligodendrogliomas independent of their 1p/19q status: a first group of 8 patients displayed significantly different 3D telomere profiles between both surgeries (p < 0.0001). Their recurrence happened at a mean of 231.375 ± 117.42 days and a median time to progression (TTP) of 239 days, a period defined as short-term recurrence; and a second group of three patients displayed identical 3D telomere profiles between both surgery samples (p > 0.05). Their recurrence happened at a mean of 960.666 ± 86.19 days and a median TTP of 930 days, a period defined as long-term recurrence. Our results suggest a potential link between nuclear telomere architecture and telomere dysfunction with time to recurrence in oligodendrogliomas, independently of the 1p/19q status.

scholarly journals Real-time in situ three-dimensional integral videography and surgical navigation using augmented reality: a pilot study

2013 ◽  
Vol 5 (2) ◽  
pp. 98-102 ◽  
Author(s):  
Hideyuki Suenaga ◽  
Huy Hoang Tran ◽  
Hongen Liao ◽  
Ken Masamune ◽  
Takeyoshi Dohi ◽  
...  
Keyword(s):  
Pilot Study ◽  
Augmented Reality ◽  
Real Time ◽  
Surgical Navigation ◽  
Three Dimensional ◽  
Dimensional Integral

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

In situ investigation of the primary recrystallization of alpha titanium in HVEM

1978 ◽  
Vol 36 (1) ◽  
pp. 634-635
Author(s):  
S. Naka ◽  
R. Penelle ◽  
R. Valle
Keyword(s):  
Dimensional Analysis ◽  
Texture Evolution ◽  
Cold Work ◽  
Three Dimensional ◽  
Primary Recrystallization ◽  
Thin Foils ◽  
In Situ Investigation ◽  
Grain Growth Model ◽  
Alpha Titanium

The in situ experimentation technique in HVEM seems to be particularly suitable to clarify the processes involved in recrystallization. The material under investigation was unidirectionally cold-rolled titanium of commercial purity. The problem was approached in two different ways. The three-dimensional analysis of textures was used to describe the texture evolution during the primary recrystallization. Observations of bulk-annealed specimens or thin foils annealed in the microscope were also made in order to provide information concerning the mechanisms involved in the formation of new grains. In contrast to the already published work on titanium, this investigation takes into consideration different values of the cold-work ratio, the temperature and the annealing time.Two different models are commonly used to explain the recrystallization textures i.e. the selective grain growth model (Beck) or the oriented nucleation model (Burgers). The three-dimensional analysis of both the rolling and recrystallization textures was performed to identify the mechanismsl involved in the recrystallization of titanium.

Detection and mapping of interactions between chromatin and the nuclear envelope in drosophila embryos

1995 ◽  
Vol 53 ◽  
pp. 764-765
Author(s):  
W.F. Marshall ◽  
A.F. Dernburg ◽  
B. Harmon ◽  
J.W. Sedat
Keyword(s):  
Nuclear Envelope ◽  
Chromatin Condensation ◽  
Three Dimensional ◽  
Physiological Role ◽  
Nuclear Surface ◽  
Intact Cells ◽  
Molecular Determinants ◽  
Surface Harmonic ◽  
Drosophila Embryos

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

Wątek składania ofiar w dekoracji budowli sakralnych Dura Europos

Vox Patrum ◽  
2015 ◽  
Vol 64 ◽  
pp. 393-402
Author(s):  
Ilona Skupińska-Løvset
Keyword(s):  
Roman Empire ◽  
Late Antiquity ◽  
Archaeological Research ◽  
Dominant Form ◽  
Xxth Century ◽  
Desert Sand ◽  
Military Camp ◽  
The Military

Dura Europos, or as proposed today Europos Dura, was a fortified settlement on the border between the Roman Empire and the East. The archeological dis­coveries reflected the character of the settlement – the fortified agglomeration grouped at the military camp. After its fall Europos Dura was covered by desert sand only to be discovered in the XXth century. Archaeological research has dis­closed documentation of its multicultural character. This paper points to the fact of coexistence of various religions in late antiquity Europos Dura. Paintings and sculptures discovered in situ indicate that scene of offering was a favorite subject in the sacral art of Europos Dura, independent of religion. The ceremony of in­cense burning constitutes the dominant form of offering regarding visualizations of this important ceremony.

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