Microscopic Features and Preliminary Thin Layer Chromatography of “Thanh ngam” (Picria fel-terrae Lour. ex Wall) Collected in Viet Nam

2017 ◽  
Vol 1 (6) ◽  
pp. 241-243
Author(s):  
Nguyen Tung ◽  
◽  
Nguyen Than
Author(s):  
Sushilkumar Shinde ◽  
Sarin Chavhan ◽  
Saurabh Jain ◽  
Karunakar Shukla

Aim: The present work was aimed at evolving pharmacognostical and phytochemical quality standards for certain traditional herbs like Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum. These three plants are reported to possess antidiabetic activity. Study Design: The plants were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) Place and Duration of Study: The study was carried out at College of Pharmacy, Dr. APJ Abdul Kalam University, Indore, during 2019-20 Methodology: The material were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis,  phytochemical properties, and thin layer chromatography (TLC) profile of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum were determined using standard methods. Results: The macroscopical and microscopical studies revealed useful diagnostic features. Phytochemical screening reveals the presence of secondary metabolites, physicochemical including fluorescence analysis of powdered drug proved useful to differentiate the powdered drug material. Thin layer chromatography analysis showed the presence of important phytoconstituents such Phyllanthin, hypophyllanthin, glycerrizin and piperine Conclusion: The data generated from this study would serve as useful gauge for determining the quality of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum thereby correct identification and authentification of these plants. It would also help scientists to utilize such needful information regarding the plants identity and characteristics in building new polyherbal formulations.


2018 ◽  
Vol 4 (2) ◽  
pp. 60-65
Author(s):  
Nguyen Thanh Tung ◽  
◽  
Nong Phuong Anh ◽  
Ho Thu Trang ◽  
Nguyen Van Quan ◽  
...  

The objective of this study is to determine the morphological characteristics of flowers and pollen, preliminary phytochemical evaluation and thin layer chromatography of five species of genus Balanophora J.R. & G. Forst collected in Viet Nam: Balanophora fungosa J.R. & G. Forst ssp. indica (Arn.) Hansen var. indica, Balanophora fungosa J.R. & G. Forst ssp. indica (Arn.) Hansen var. globosa (Jungh.) Hansen, Balanophora latisepala (Tiegh) Lecome, Balanophora laxiflora Hemsl, Balanophora subcupularis P.C. Tam. The morphological characteristics of male flowers, female flowers and spadicles, pollen grain of five species were described. Results of preliminary phytochemical evaluation of ethanol extracts of five species showed that all of five species have tannins, flavonoids, coumarins, sterols. Thin layer chromatography of ethanol extracts of five species were developed and base on TLC chromatogram, it is easy to differentiate these species.


Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.


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