scholarly journals Pharmacognostical Study, Phytochemical and Physicochemical Evaluation and Establishment of Quality Standards for Certain Traditional Antidiabetic Medicinal Plants

2021 ◽  
pp. 288-299
Author(s):  
Sushilkumar Shinde ◽  
Sarin Chavhan ◽  
Saurabh Jain ◽  
Karunakar Shukla
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescence Analysis ◽  
Quality Standards ◽  
Piper Nigrum ◽  
Phyllanthus Amarus ◽  
Qualitative And Quantitative ◽  
Microscopic Features ◽  
Layer Chromatography ◽  
Powdered Drug

Aim: The present work was aimed at evolving pharmacognostical and phytochemical quality standards for certain traditional herbs like Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum. These three plants are reported to possess antidiabetic activity. Study Design: The plants were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) Place and Duration of Study: The study was carried out at College of Pharmacy, Dr. APJ Abdul Kalam University, Indore, during 2019-20 Methodology: The material were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis,  phytochemical properties, and thin layer chromatography (TLC) profile of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum were determined using standard methods. Results: The macroscopical and microscopical studies revealed useful diagnostic features. Phytochemical screening reveals the presence of secondary metabolites, physicochemical including fluorescence analysis of powdered drug proved useful to differentiate the powdered drug material. Thin layer chromatography analysis showed the presence of important phytoconstituents such Phyllanthin, hypophyllanthin, glycerrizin and piperine Conclusion: The data generated from this study would serve as useful gauge for determining the quality of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum thereby correct identification and authentification of these plants. It would also help scientists to utilize such needful information regarding the plants identity and characteristics in building new polyherbal formulations.

scholarly journals High-Performance Thin-Layer Chromatography Densitometric Method for Simultaneous Quantitation of Phyllanthin, Hypophyllanthin, Gallic Acid, and Ellagic Acid in Phyllanthus amarus

2006 ◽  
Vol 89 (3) ◽  
pp. 619-623 ◽  
Author(s):  
Kamlesh Dhalwal ◽  
Yogesh S Biradar ◽  
Mandapati Rajani
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
Ellagic Acid ◽  
High Performance ◽  
Raw Material ◽  
Phyllanthus Amarus ◽  
Whole Plant ◽  
Recovery Study ◽  
Layer Chromatography

Abstract Whole plant of Phyllanthus amarus Linn. is a reputed drug of the Indian systems of medicine that is used as hepatoprotective agent. A simple high-performance thin-layer chromatography (HPTLC) densitometric method has been developed for the simultaneous quantitation of phyllanthin, hypophyllanthin, gallic acid, and ellagic acid in the whole plant of P. amarus. They were found at levels of 0.37, 1.16, 0.36, and 0.17% (w/w), respectively. The method was validated for precision, repeatability, and accuracy. Instrumental precision was found to be 0.54, 0.93, 0.08, and 0.78% (coefficient of variation, CV); repeatability of the method was 1.01, 0.79, 0.98, and 1.06% (CV) for phyllanthin, hypophyllanthin, gallic acid, and ellagic acid, respectively. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was found to be 99.09% for phyllanthin, 99.27% for hypophyllanthin, 98.69% for gallic acid, and 100.49% for ellagic acid. The proposed HPTLC method was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control of raw material of P. amarus and formulations containing P. amarus. It also has the applicability in quantitating any of these marker compounds in other drugs.

Urinary Homogentisic Acid: Determination by Thin-Layer Chromatography

1973 ◽  
Vol 19 (5) ◽  
pp. 459-462 ◽  
Author(s):  
Jerome M Feldman ◽  
June Bowman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Laboratory ◽  
Homogentisic Acid ◽  
Qualitative And Quantitative ◽  
Color Development ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Acid Determination

Abstract A new method is described for qualitative and quantitative determination of urinary homogentisic acid. The method involves extraction of urine with ethyl acetate, thin-layer chromatography of the extract on Silica Gel G, elution of the homogentisic acid into water, and color development with Folin’s phenol reagent. Absorbance is maximum at 750 nm and linear to a concentration of at least 5 mg of homogentisic acid per milliliter of urine. The method is highly specific for homogentisic acid; added gentisic acid, 3,4-dihydroxyphenylacetic acid, ascorbic acid, or L-3,4 dihydroxyphenylalanine do not interfere. The coefficient of variation "in-run" is 5.3%, "between-run" 6.8%. Using this method we have demonstrated a marked variation in day-to-day homogentisic acid excretion in a patient with alcaptonuria. This method, which offers some advantages over existing techniques, should be suitable for use in a clinical laboratory.

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