Phytochemical Constituents and Antimicrobial Activity of Elaeocarpus sphaericus Schum Seed Extract

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

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Antidiabetic Activity and Phytochemical Constituents of Syzygium cumini Seeds in Puducherry Region, South India

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i07.11168 ◽

2018 ◽

Vol 9 (07) ◽

Cited By ~ 1

Author(s):

Kandan Prabakaran ◽

Govindan Shanmugave

Keyword(s):

Inhibitory Effect ◽

Seed Extract ◽

Antidiabetic Activity ◽

Appreciable Amount ◽

Syzygium Cumini ◽

Characteristic Functional ◽

Infrared Spectral ◽

Phytochemical Constituents ◽

Dose Dependent Increase ◽

Dose Dependent

Syzygium cumini is widely used in traditional medicine to treat diabetes in India. The present study was carried out to evaluate the phytochemical bioactive compounds from Syzygium cumini seed extract and its invitro anti-diabetic activity. The phytochemical screening showed appreciable amount of flavonoid and steroid in the seed extract. The infrared spectral data obtained revealed the presence of characteristic functional groups of alcohol, hydroxyl, aldehydes, alkanes, alkenes, nitro compound and aliphatic amines etc. The extract exhibits the dose-dependent increase in the inhibitory effect on alphaamylase enzyme upto 95.4%. The result suggested that significant amount of flavonoid in Syzygium cumini seed is responsible for antidiabetic properties and it is further confirmed by higher intensity of alpha amylase inhibitory effect.

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Effectiveness of grape seed extract-based intracanal dressings against Enterococcus Faecalis and its influence on dentin microhardness and bond strength of filling material

Bioscience Journal ◽

10.14393/bj-v36n6a2020-54066 ◽

2020 ◽

Vol 36 (6) ◽

Author(s):

Matheus Albino Souza ◽

Daniel De Lima Dalla Lana ◽

Amália Pletsch ◽

Huriel Scartazzini Palhano ◽

Juliane Bervian ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bond Strength ◽

Enterococcus Faecalis ◽

Storage Period ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Filling Material ◽

Bacterial Reduction ◽

Significant Difference

The aim of this study was to evaluate the effectiveness of grape seed extract (GSE)-based intracanal dressings against Enterococcus faecalis (E. faecalis) and its influence on dentin microhardness and bond strength of the filling material. The root canals of 126 human teeth were distributed into three test groups: antimicrobial activity (60 teeth), dentin microhardness (30 teeth) and bond strength (36 teeth). In all three groups, specimens were subdivided into six groups, according to intracanal dressing protocols: G1 – distilled water (DW); G2 – 2% chlorhexidine gel (CHX); G3 – calcium hydroxide (Ca[OH]2)+DW; G4 – GSE+DW; G5 – Ca(OH)2+CHX; G6 – GSE+CHX. The counting of colony-forming units (CFUs), the Vickers microhardness tester and the push-out test were performed to evaluate the antimicrobial activity, dentin microhardness and bond strength, respectively. Specific statistical analysis was performed for each evaluation (α=5%). The greatest bacterial reduction was observed in G5 (Ca[OH]2+CHX) and G6 (GSE+CHX) (p<0.05). There was no statistically significant difference among groups in the dentin microhardness evaluation (p<0.05). The highest bond strength in the immediate evaluation was observed in G4 (GSE+DW) and G6 (GSE+CHX), whereas the highest bond strength after 12 months of storage was observed in G2 (CHX), G3 (Ca[OH]2+DW), G4 (GSE+DW), and G6 (GSE+CHX) (p<0.05). After the storage period, bond strength was increased in G2 (CHX) and G3 (Ca[OH]2+DW), and remained unchanged in G4 (GSE+DW) and G6 (GSE+CHX) (p<0.05). GSE-based intracanal dressings have antimicrobial potential against E. faecalis, have no influence in dentin microhardness and preserve the high bond strength of filling materials for root dentin over time.

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