Investigation on Phytochemical Constituents and Antimicrobial Activity of Methanolic Extract of Sonneratia apetala Buch-Ham. Areal Parts

2021 ◽  
pp. 121-127
Author(s):  
Sangram Keshari Panda ◽  
Debasis Pati ◽  
S. K. Mishra ◽  
S. Sahu ◽  
B. Tripathy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Sonneratia Apetala ◽  
Phytochemical Constituents

scholarly journals ANTIMICROBIAL ACTIVITY OF LEMON PEEL (CITRUS LIMON) EXTRACT

2017 ◽  
Vol 9 (4) ◽  
pp. 79 ◽  
Author(s):  
Junab Ali ◽  
Biswajit Das ◽  
Trideep Saikia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Citrus Limon ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Standard Methods ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) in conjugation with phytochemical analysis.Methods: The methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) was separated from fruits, shade dried, powdered and extracted using methanol, analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is Gram-positive (Staphylococcus aureus) and other is Gram-negative (Escherichia coli) and 1 fungal strains (Candida albicans) using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the methanolic extract of the fruits peel Citrus limon. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides and citric acids and tannins. The antimicrobial activity of the methanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that methanolic extract of the peel of Citrus Limon contains the high presence of phytochemicals. The methanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards. 

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DRIED FLOWERS OF ADENIUM OBESUM

2019 ◽  
pp. 34-36
Author(s):  
SIREESHA KALVA ◽  
RAGHUNANDAN N.
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Inhibition Concentration ◽  
Test Organisms ◽  
Phytochemical Constituents

Objective: To estimate the antimicrobial activity of methanolic extract of Adenium obesum (family Apocyanaceae) in association with phytochemical analysis. Methods: The methanolic extract of the flowers of Adenium obesum was prepared and investigated for phytochemical constituents using standard methods. Antimicrobial activity of methanolic extract Adenium obesum has been carried out against one gram-positive bacteria Staphylococcus aureus and one gram-negative bacteria Escherichia coli and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of methanolic extract was compared with that of standard drug i.e., Ciprofloxacin for antibacterial activity and Fluconazole for antifungal activity. The minimum inhibition concentration was determined by micro dilution method (cup-plate method). Results: The present investigation shows the phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenols, proteins and amino acids, cardiac glycosides, steroids, tannin, and phenolic compounds. The anti-microbial activity of the methanolic extract of the plant showed significant results against all the three of the test organisms in compare to the standard drugs. Conclusion: The present study concluded that methanolic extract of the flowers of adenium obesum proved the existence of phytochemical constituents. The methanolic extract of the was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of antibiotics.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DRIED FLOWERS OF ADENIUM OBESUM

2019 ◽  
pp. 34-36
Author(s):  
SIREESHA KALVA ◽  
RAGHUNANDAN N.
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Inhibition Concentration ◽  
Test Organisms ◽  
Phytochemical Constituents

Objective: To estimate the antimicrobial activity of methanolic extract of Adenium obesum (family Apocyanaceae) in association with phytochemical analysis. Methods: The methanolic extract of the flowers of Adenium obesum was prepared and investigated for phytochemical constituents using standard methods. Antimicrobial activity of methanolic extract Adenium obesum has been carried out against one gram-positive bacteria Staphylococcus aureus and one gram-negative bacteria Escherichia coli and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of methanolic extract was compared with that of standard drug i.e., Ciprofloxacin for antibacterial activity and Fluconazole for antifungal activity. The minimum inhibition concentration was determined by micro dilution method (cup-plate method). Results: The present investigation shows the phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenols, proteins and amino acids, cardiac glycosides, steroids, tannin, and phenolic compounds. The anti-microbial activity of the methanolic extract of the plant showed significant results against all the three of the test organisms in compare to the standard drugs. Conclusion: The present study concluded that methanolic extract of the flowers of adenium obesum proved the existence of phytochemical constituents. The methanolic extract of the was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of antibiotics.

Phytochemical Profiling, Pharmacological activities, Isolation and Identification of Phytochemicals from Sterculia urens Roxb. leaves

2021 ◽  
Vol 25 (11) ◽  
pp. 11-19
Author(s):  
Chaitanya Darapureddy ◽  
K.R.S. Prasad ◽  
R.S. Ch Phani
Keyword(s):  
Phenolic Compounds ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Clot Lysis ◽  
Inhibition Activity ◽  
Pharmacological Activities ◽  
Sterculia Urens ◽  
Phytochemical Constituents

The study is intended to evaluate the pharmacological activities, isolation and characterization of the bioactive phytochemical constituents from the crude leaf extract of Sterculia urens Roxb. The extraction of the phytochemicals from the leaves was carried on Soxhlet extraction apparatus using solvents like hexane, ethyl acetate, methanol and water. Pharmacological activities such as DPPH free radical scavenging assay (antioxidant), α-amylase inhibition assay (antidiabetic), albumin denaturation assay (antiinflammatory), blood clot lysis method (thrombolytic) and well diffusion method (antibacterial) of the crude extracts were evaluated and then the semi-preparative HPLC analysis followed by spectral studies was carried for the purification and identification of bioactive compounds. The methanolic extract showed high DPPH radical scavenging activity, α-amylase inhibition activity and albumin denaturation inhibition activity with IC50 values of 29.51±0.11, 146.85±0.18 and 149.91±0.19 μg/mL respectively. The % clot lysis of the methanolic extract was found higher than the other extracts and all the extracts have potential inhibition on the growth of the bacterial studied. From the leaf methanolic extract, 4 phenolic compounds (2,4-dihydroxybenzoic acid, methyl 4- hydroxycinnamate, p-coumaric acid and stercurensin) and 3 phenolic compounds (gossypetin, farrerol and quercetin 5,7,3',4'-tetramethyl ether) were isolated and characterised. Based on the results observed, it can be concluded that the leaf extracts of Sterculia urens Roxb are having rich phytochemical constituents with prominent pharmacological activities.

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 64-67
Author(s):  
Maruthi T. Ekbote ◽  
◽  
Rajashekar K. V ◽  
Shankarappa L ◽  
Bharathi D. R. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Acorus Calamus ◽  
Aqueous Extracts ◽  
Piper Longum ◽  
Polyherbal Formulation ◽  
Methanolic Extracts ◽  
Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

scholarly journals Comparative Study on the Antimicrobial Efficacy of Methanolic Extract of Root, Callus and Fruit Extracts of Myxopyrum smilacifolium Blume

2014 ◽  
Vol 2 (4) ◽  
pp. 521-524
Author(s):  
RP Praveen ◽  
Ashalantha Nair
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Fungal Strain ◽  
Antimicrobial Efficacy ◽  
Bacterial Strains ◽  
E Coli ◽  
Root Extracts ◽  
Fruit Extracts ◽  
Root Callus ◽  
And Staphylococcus Aureus

The aim of the present study was to compare the antimicrobial efficacy of methanolic extract of root, callus and fruit of Myxopyrum smilacifolium Blume. Antimicrobial activity was tested using agar well diffusion with four bacterial strains viz: Escherechia coli, Enterococcus faecalis, Bacillus subtilis and Staphylococcus aureus of which E. coli alone was gram negative. The fungal strain employed was Candida albicans. Root extracts shown to be effective only against B. subtilis. Fruit extracts showed the maximum antimicrobial activity against all the microbial species considered for the current study except against S. aureus. Highlight of the present study was the antimicrobial activity of callus extracts. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11362  Int J Appl Sci Biotechnol, Vol. 2(4): 521-524 

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

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