Experimental and morphological reasoning of glutinous fixation of polypropylene mesh implant to the muscles of the abdominal wall

PURPOSE: To verify if the composit poli (2-hydroxyethyl methacrylate)-PolyHEMA/polypropylene mesh implanted in the female rat's abdominal wall could be suitable for the prevention of peritoneal adhesions, and for the evaluation of the tecidual response produced by this biomaterial. METHODS: Polypropylene meshes (Group PP, n=20) and polypropylene meshes coated with a layer of poli (2-hydroxyethyl methacrylate)-PolyHEMA (Group PH, n=20) were implanted on the abdominal wall of Wistar female rats. Ten animals from each group were submitted to euthanasia at 15 and 30 days of the postoperative period. RESULTS: The animals from the group PP presented visceral adhesions on the mesh surface, which was not observed in the ones from group PH. At the histopathological examination foreign body response was observed in both groups, whilst there was a greater intensity of inflammatory response in group PH on both moments. CONCLUSION: The poli (2-hydroxyethyl methacrylate) polyHEMA hydrogel associated to polypropylene mesh reduces visceral adhesion formation in rats, although it may be associated to greater inflammatory reaction.

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ESTIMATION OF THE EFFECT OF PLATELET RICH PLASMA PRODUCTS IN THE INTEGRATION OF POLYPROPYLENE MESH IMPLANT IN BIOLOGICAL TISSUES. EXPERIMENTAL MODEL IN RATS

EUREKA Health Sciences ◽

10.21303/2504-5679.2018.00736 ◽

2018 ◽

Vol 5 ◽

pp. 11-19

Author(s):

Dmitro Atanasov

Keyword(s):

Stem Cells ◽

Adipose Tissue ◽

Polypropylene Mesh ◽

Morphological Changes ◽

Platelet Rich Plasma ◽

Biological Tissues ◽

Tissue Reaction ◽

Plasma Cytokines ◽

Mesh Implant ◽

Positive Effect

The aim of the research is to determine morphological changes in the area of implantation of the polypropylene mesh implant and to determine the effect on the integration of the prosthesis of locally introduced adipose tissue and platelet rich plasma. Materials and methods. The experiment was performed on 36 sexually mature males of the Wistar line rats. The experiment simulated, studied and quantified local morphological responses and changes in developing in biological tissues that are in contact with implanted highly porous lightweight (80 g/m2) mesh implant in isolation and also in conditions of local administration of fatty graft and platelet rich plasma. Results: Assuming introduction of adipose tissue and platelet rich plasma in the zone of integration of mesh alloprosthesis under the influence of introduced regenerative cytokines as well as stromal stem cells activated by them there is an earlier activation of regenerative processes, enhanced angiogenesis which determines the optimal nature of the integration of the prosthesis with the formation of thin collagen fibers in more early terms minimizing excess peri-prosthetic fibrosis. Isolated introduction into the implantation zone of fatty suspension determines similar changes that have a slightly less pronounced character. These changes are quantitatively studied and the results obtained are statistically significant. Conclusions: Applying a fatty graft together with platelet rich plasma in the area of implantation of the lung polypropylene prosthesis, there was an accelerated tissue reaction from the integration of the prosthesis. Mesenchymal stem cells of adipose tissue that is a target for plasma cytokines enriched with thrombocytes have a more pronounced effect in stimulating reparative processes provided that they are simultaneously administered with PRP compared with isolated administration without PRP. The use of platelet rich plasma and adipose tissue design has a significant positive effect on local angiogenesis. Under conditions of improved angiogenesis and other stimulating factors in the conditions of introduction of adipose tissue and PRP, the integration of the prosthesis occurs with significantly lower peri-prosthetic fibrosis.

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Functional and morphological evaluation of different polypropylene-mesh modifications for abdominal wall repair

Biomaterials ◽

10.1016/s0142-9612(98)00115-x ◽

1998 ◽

Vol 19 (24) ◽

pp. 2235-2246 ◽

Cited By ~ 126

Author(s):

B Klosterhalfen

Keyword(s):

Abdominal Wall ◽

Polypropylene Mesh ◽

Abdominal Wall Repair ◽

Morphological Evaluation

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Invited Commentary on The Analysis of Infection after Polypropylene Mesh Repair of Abdominal Wall Hernia

World Journal of Surgery ◽

10.1007/s00268-006-0478-6 ◽

2006 ◽

Vol 30 (12) ◽

pp. 2279-2280

Author(s):

Roger Saadia

Keyword(s):

Abdominal Wall ◽

Polypropylene Mesh ◽

Mesh Repair ◽

Abdominal Wall Hernia

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The optical analysis of the abdominal wall using the biospeckle after implants of polypropylene mesh in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502009000600004 ◽

2009 ◽

Vol 24 (6) ◽

pp. 442-448

Author(s):

Tâmara Maria Nieri ◽

Marco Antonio de Oliveira Peres ◽

Emerson Rodrigo da Silva ◽

Inácio Maria Dal Fabbro ◽

Mikiya Muramatsu ◽

...

Keyword(s):

Animal Model ◽

Abdominal Wall ◽

Polypropylene Mesh ◽

Male Rats ◽

Mesh Placement ◽

Group 4 ◽

Mesh Prosthesis ◽

Group 3 ◽

Group 2 ◽

Group 1

PURPOSE: To evaluate an experimental animal model to study the abdominal tissue activity considering its interaction with a polypropylene mesh, through the use of one of the optical phenomena of light Laser, the biospeckle. METHODS: Fifty Wistar male rats were divided into four groups: Group 1: ten animals not submitted to surgery; Group 2: ten animals submitted to surgery without polypropylene mesh; Group 3: 20 animals submitted to surgery followed by the mesh placement; Group 4: (sham) with ten animals. None of the animals presented post surgical complications being submitted to the optical tests at the 20th postoperative day. RESULTS: The analysis from the biospeckle tests, comparing the medians and standard deviations with T Student test, indicated that no significative difference was observed on the abdominal wall tissue activity in the four groups considered, with and without polypropylene mesh prosthesis implantation. CONCLUSION: The animal model is viable and the biospeckle open ways for a great number of experiments to be developed in evaluating tissue activity.

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