Development of Seed Vigour Testing Method Using Single Count of Radicle Emergence for True Seed of Shallot (Allium ascalonicum B.)

The effect of two priming techniques: hardening (prehydration treatment) and solid matrix priming (SMP) was studied on the seeds of two parsley varieties (Cukrowa and Berlińska) in 3-year laboratory experiments. On the images obtained from scanning electron microscope (SEM) there is a parsley embryo developing during germination up to the moment of radicle emergence. On the surface of primed seeds, in particular using the hardening method, lateral cracks are visible. The respiratory activity of primed seeds was similar to that of non-primed in the period of initial 24 h of germination, but significantly higher after 48 h. As compared to non primed seeds solid matrix priming significantly increased the percentage and the speed of germination. Nevertheless, pelleting reduced the positive effect of priming on the germination ability, without affecting the germination speed. After 18 months of storage, the vigour of primed seeds, particularly through hardening, had significantly decreased.

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Seed Vigour Tests to Estimate Seedling Emergence in Cress (Lepidium sativum L.) Seed Lots

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47311453 ◽

2019 ◽

Vol 47 (3) ◽

Author(s):

Ibrahim DEMIR ◽

Burcu B. KENANOGLU ◽

Eren ÖZDEN

Keyword(s):

Electrical Conductivity ◽

Seedling Emergence ◽

Accelerated Aging ◽

Lepidium Sativum ◽

Accelerated Ageing ◽

Digital Object Identifier ◽

Seed Vigour ◽

Significance Levels ◽

Radicle Emergence ◽

Lepidium Sativum L

This work was carried out to estimate field and controlled room seedling emergence potential through seed vigour tests in cress (Lepidium sativum L.) seeds. Early radicle emergence percentages after 12 (RE12h), 24 (RE24h) and 36 (RE36h) hours of germination test, mean germination time, accelerated aging (45 °C, 100% RH, 24 h), electrical conductivity (EC) of soaking water (40 ml, 50 seeds, 20 °C), after 16 hours (EC16h) and 24 hours (EC24h), and EC16h and EC24h after accelerated ageing (AA, 45 °C, 100% RH, 24h) were tested as vigour tests in ten commercial seed lots of cress. Standard laboratory germination ranged between 88 and 93%. Seeds were sown on two occasions in field and controlled room conditions, and seedling emergence percentages were determined after 30 days in the soil and 14 days in the controlled room. Seedling emergence ranged between 67 and 85% and 59 and 83% in the first and second sowings in the field. These values were 75 and 92% in controlled room sowing. Vigour tests were correlated to seedling emergence potential at various significance levels but RE24h and EC16h showed the highest correlation coefficient values (p < 0.001) in all three sowing conditions as r = 0.879-0.988 in RE24h, and r = 0.902-0.962 in EC16h. Results indicated that early radicle emergence percentages after 24 hours (RE24h) and electrical conductivity value after 16 hours (EC16h) can be successfully used to estimate the seedling emergence potential of cress seeds in field and controlled room conditions. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Single counts of radicle emergence can be used as a fast method to test seed vigour of indica rice

Seed Science and Technology ◽

10.15258/sst.2017.45.1.19 ◽

2017 ◽

Vol 45 (1) ◽

pp. 222-229 ◽

Cited By ~ 1

Author(s):

Y. Luo ◽

C. Lin ◽

Y.T. Huang ◽

Y.J. Guan ◽

J. Hu

Keyword(s):

Indica Rice ◽

Fast Method ◽

Seed Vigour ◽

Radicle Emergence

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Study of ethylene kinetics during and after germination of sugar beet (Beta vulgarisL.) seeds and fruits

Seed Science Research ◽

10.1017/s0960258513000147 ◽

2013 ◽

Vol 23 (3) ◽

pp. 205-210 ◽

Cited By ~ 5

Author(s):

Willem Abts ◽

Carolien Vissers ◽

Bert Vandenbussche ◽

Maurice M.P. De Proft

Keyword(s):

Sugar Beet ◽

Ethylene Production ◽

Isobutyric Acid ◽

Time Span ◽

Aminooxyacetic Acid ◽

Silver Thiosulphate ◽

True Seed ◽

Radicle Emergence ◽

Amino Isobutyric Acid ◽

Seeds And Fruits

AbstractThe interaction between ethylene production and seed germination of sugar beet (Beta vulgarisL.) was studied. For intact fruits, deoperculated fruits and true seeds, ethylene was only produced after the start of radicle emergence. Removal of the operculum or the whole pericarp, likely allowing better water uptake and gas exchange by the true seed, actually increased the time span between the start of radicle emergence and the beginning of ethylene production compared to intact fruits. ACC (1-aminocyclopropane-1-carboxylic acid), AOA (aminooxyacetic acid), AIB (2-amino isobutyric acid) and STS (silver thiosulphate) in the imbibition medium did not influence the germination pattern. Based on these findings, the function of ethylene during the germination of sugar beet is uncertain.

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Seed quality, cotyledon elongation at suboptimal temperatures, and the yield of onion

Seed Science Research ◽

10.1017/s0960258500000647 ◽

1991 ◽

Vol 1 (1) ◽

pp. 57-67 ◽

Cited By ~ 12

Author(s):

T. R. Wheeler ◽

R. H. Ellis

Keyword(s):

Allium Cepa ◽

Crop Yield ◽

Seedling Growth ◽

Seed Quality ◽

Plant Size ◽

Base Temperature ◽

Seed Vigour ◽

Plant Weight ◽

Radicle Emergence ◽

The Mean

AbstractCotyledon length in onion (Allium cepa, cv. White Lisbon) was linearly related to time from radicle emergence until the formation of the cotyledon hook, but thereafter increased exponentially with time. Within each phase the rate of cotyledon elongation was linearly related to temperature, and these relations were similar among seeds from several percentiles of the germinating populations of three contrasting seed lots. The mean base temperature was 1.4°C. These thermal time relations closely predicted the elongation of cotyledons of seedlings from cohorts of early- and lategerminators from a further lot of this cultivar at two suboptimal temperatures. Eleven cohorts of seedlings from seeds of differing quality were transplanted to the field on both the same date and at the same time from visible germination. The plants were harvested 59 days later. No effect of seed quality on mean plant weight, mean bulb diameter, or interplant variation in bulb diameter was detected. It is concluded that the rate of pre- and post-emergence seedling growth in onion is independent of environmentally induced differences in seed quality (seed vigour). Thus, any effect of this factor on plant size or crop yield, at a given density, is solely a function of time to germinate.

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Evaluation of Radicle Emergence Test to Predict Seed Vigour and Field Emergence in Okra (Abelmoschus Esculentus L.) Seeds

International Journal of Agricultural Science and Research ◽

10.24247/ijasrjun201937 ◽

2019 ◽

Vol 9 (3) ◽

pp. 247-258

Author(s):

G. P. Chinnasamy et al., G. P. Chinnasamy et al., ◽

Keyword(s):

Abelmoschus Esculentus ◽

Seed Vigour ◽

Radicle Emergence ◽

Field Emergence

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Seed vigour evaluation in petunia seed lots to predict seedling emergence and longevity

Seed Science and Technology ◽

10.15258/sst.2020.48.3.07 ◽

2020 ◽

Vol 48 (3) ◽

pp. 391-400

Author(s):

Ibrahim Demir ◽

Nihal Erturk ◽

Zeynep Gokdas

Keyword(s):

Electrical Conductivity ◽

Seedling Emergence ◽

Accelerated Ageing ◽

Seed Vigour ◽

Germination Time ◽

Ageing Test ◽

Radicle Emergence ◽

Mean Germination Time ◽

Storage Potential ◽

And Storage

This study examined the potential of different vigour tests to reveal differences in performance of nine hybrid petunia seed lots. Saturated salt accelerated ageing test (SSAA) and 48-hour radicle emergence (RE48h) tests were able to predict seedling emergence and longevity potential. The results of accelerated ageing (AA) (at 41/43°C, 48/72 hours, 100% RH), saturated salt accelerated ageing (SSAA) (at 41/43/45°C, 72 hours, 75% RH), mean germination time (MGT), 48-hour radicle emergence (RE48h) and electrical conductivity (EC) tests were compared with seedling emergence in modules and longevity (at 5°C for 24 months and 25°C for 12 months). Initial standard germination (SG) percentages were above 83% in the lots. Seedling emergence ranged between 18 and 89%, germination after storage was between 16 and 88% at 5°C, and 13 and 96% at 25°C. The SSAA test at 43°C and 45°C for 72 hours was correlated with both seedling emergence and longevity. RE48h was correlated with emergence and germination after storage at 5°C for 24 months; MGT and SG was correlated with seedling emergence. Neither AA nor the EC test was correlated with emergence and longevity. Thus vigour, expressed as both emergence and storage potential, was successfully predicted by the SSAA test and RE48h.

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Early counts of radicle emergence, counted manually and by image analysis, can reveal differences in the production of normal seedlings and the vigour of seed lots of cauliflower

Seed Science and Technology ◽

10.15258/sst.2021.49.3.04 ◽

2021 ◽

Author(s):

Takashi Shinohara ◽

Sylvie Ducournau ◽

Stan Matthews ◽

Marie-Helene Wagner ◽

Alison A. Powell

Keyword(s):

Image Analysis ◽

Seed Quality ◽

Automated Image Analysis ◽

Genotypic Differences ◽

Germination Test ◽

Seed Vigour ◽

Seed Ageing ◽

Radicle Emergence ◽

Controlled Deterioration ◽

Initial Seed

The ability of a single early count of radicle emergence (RE) to predict differences in the production of normal seedlings (NG) and seed vigour was examined in four seed lots from each of three varieties of cauliflower (Brassica oleracea var botrytis). Initial seed quality was assessed in germination and controlled deterioration (CD) vigour tests. RE (first appearance of the radicle) was counted manually after 48 hours in the germination test (RE 48h 20/30°C) and every two hours for 144 hours at 20°C using automated image analysis. Seed vigour was assessed in field and glasshouse trials. A large proportion of the variance in NG was explained by the RE count. The same RE counts at 20/30°C and 20°C predicted seed vigour in the field and glasshouse and revealed the same vigour differences as the validated CD test. Differences in vigour (field and glasshouse emergence) observed between varieties were related to the extent of previously sustained seed ageing revealed by the CD test, although genotypic differences in vigour could not be excluded. We propose that single counts of RE taken manually or by image analysis can be used to reveal differences in the NG and vigour of seed lots of cauliflower.

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Germination-related proteins in wheat revealed by differences in seed vigour

Seed Science Research ◽

10.1017/s0960258500003998 ◽

1998 ◽

Vol 8 (2) ◽

pp. 99-112 ◽

Cited By ~ 3

Author(s):

P. A. Guy ◽

M. Black

Keyword(s):

Accelerated Aging ◽

Aging Treatment ◽

Seed Vigour ◽

Normal Completion ◽

Gradual Loss ◽

Or Groups ◽

Radicle Emergence ◽

Viable Seeds ◽

Related Proteins

AbstractArtificial aging of wheat grains (Triticum aestivum cv. Mercia) for 3 d resulted in the gradual loss of seed vigour without loss of viability, but the latter decreased rapidly over the next 24 h. Loss of vigour was expressed as a delay in germination i.e. an increasing ‘lag’ period with aging before the onset of radicle emergence, but the subsequent rate of seedling growth was not affected. There was an overall decrease in the incorporation of 35S-methionine into protein in aged embryos, and associated with the aging-induced delay in the completion of germination (i.e. start of radicle emergence) there was a delay in the production of certain polypeptides. In embryos of both non-aged and aged seeds these polypeptides appeared just prior to radicle emergence but several of these were never synthesized in embryos from non-viable seeds. The synthesis of mRNA coding for in vitro translated polypeptides, or groups of polypeptides, was similarly delayed in lowvigour embryos. Furthermore, some polypeptides were identified which were induced by the accelerated aging treatment. It is concluded that a reduced level of protein and mRNA synthesis in the embryos of low-vigour seeds may result in the delay in the accumulation of specific of mRNAs and polypeptides which are needed for the normal completion of germination, i.e. radicle emergence.

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Pengujian Mutu Benih Cabai (Capsicum annuum) Dengan Metode Uji Pemunculan Radikula [Seed Quality Test in Pepper (Capsicum annuum) Seeds Using Radicle Emergence]

Jurnal Hortikultura ◽

10.21082/jhort.v29n1.2019.p9-16 ◽

2019 ◽

Vol 29 (1) ◽

pp. 9

Author(s):

Aditya Kusumawardana ◽

Bambang Pujiasmanto ◽

NFN Pardono

Keyword(s):

Capsicum Annuum ◽

Seed Quality ◽

Seed Vigour ◽

Seed Deterioration ◽

Randomized Design ◽

Radicle Emergence ◽

Vigour Index ◽

Completely Randomized Design ◽

The Relationship ◽

Pepper Seed

Kecepatan berkecambah yang rendah merupakan indikator kemunduran benih. Pengujian vigor dengan metode uji pemunculan akar pada benih cabai dilakukan untuk menduga pertumbuhan tanaman di lapangan. Makin tinggi nilai uji pemunculan akar maka vigor benih makin tinggi. Jika laju pemunculan radikula pada benih berjalan lambat, vigor benih tersebut dinyatakan rendah. Tujuan penelitian ini adalah membandingkan jumlah kemunculan radikula pada empat lot benih cabai pada suhu berganti 2030°C selama 168 jam. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor, yaitu lot benih berupa empat varietas cabai (Sret, Laskar, Serambi, dan Madun) dengan delapan ulangan. Perhitungan koefisien korelasi dilakukan untuk mengetahui keeratan hubungan antara nilai uji pemunculan radikula dengan tolok ukur pengujian yang lain. Hasil penelitian menunjukkan bahwa kemunculan radikula tertinggi terjadi pada 120 jam. Jumlah pemunculan radikula berkorelasi positif dengan daya berkecambah (r=0,907), indeks vigor (r=0,864), kecepatan tumbuh (r=0,727), dan daya tumbuh (r=0,935). Dari penelitian ini diperoleh kesimpulan bahwa uji vigor pemunculan radikula benih cabai yang dilakukan pada suhu 2030°C selama 120 jam (5 hari) dapat digunakan untuk menilai mutu benih cabai.KeywordsBenih cabai; Daya tumbuh; Mutu benih; Pemunculan radikulaAbstractLow germination is an indicator of seed deterioration. Vigour testing using radicle emergence on pepper seeds was done to predict plant growth in field. The higher radicle emergence found, the higher the seed vigour occurred. If the rate of radicle was slow, the seed vigour was also low. The objective of this study was to compare the number of radicles emergence on four pepper seed lots at 2030°C for 168 hours. This study used a completely randomized design with one factor, seed lot four variety of pepper (Sret, Laskar, Serambi, and Madun) with eight replication. Calculation of coefficients correlation was done to calculate the relationship between radicle emergence and on other testing. The highest of radicles emergence occurred at 120 hours. The number of radicle emergence had positive correlation with germination (r = 0.907), vigour index (r = 0.864), speed of growth (r = 0.727), and field emergence (r = 0.935). From this research, it can be concluded that the vigour test in pepper seeds using radicle emergence was performed at 2030°C for 120 hours (5 days).

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