Enhanced periodontal regeneration using collagen  stem cells or growth factors

Abstract Periodontal regeneration is considered to be biologically possible but clinically unpredictable. In periodontitis, inflammation manifests clinically as loss of supporting periodontal tissues and regeneration of damaged tissue is the main goal of treatment. For decades, periodontists have sought to repair the damage through a variety of surgical procedures, and use of grafting materials and growth factors, and of barrier membranes. Reports have emerged that demonstrate which populations of adult stem cells reside in the periodontal ligaments of humans and other animals. This opens the way for new cell-based therapies for perio-dontal regeneration. This review provides an overview of adult human stem cells and their potential use in perio-dontal regeneration.

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Preliminary Findings of α-Actinin-3 Gene Distribution in Elite Turkish Wind Surfers

Balkan Journal of Medical Genetics ◽

10.2478/bjmg-2013-0020 ◽

2013 ◽

Vol 16 (1) ◽

pp. 69-72 ◽

Cited By ~ 2

Author(s):

K Ulucan ◽

S Göle ◽

N Altindas ◽

A.I. Güney

Keyword(s):

Stem Cells ◽

Growth Factors ◽

Surgical Procedures ◽

Adult Stem Cells ◽

Periodontal Regeneration ◽

Human Stem Cells ◽

Barrier Membranes ◽

Periodontal Tissues ◽

Grafting Materials ◽

Potential Use

Abstract Periodontal regeneration is considered to be biologically possible but clinically unpredictable. In periodontitis, inflammation manifests clinically as loss of supporting periodontal tissues and regeneration of damaged tissue is the main goal of treatment. For decades, periodontists have sought to repair the damage through a variety of surgical procedures, and use of grafting materials and growth factors, and of barrier membranes. Reports have emerged that demonstrate which populations of adult stem cells reside in the periodontal ligaments of humans and other animals. This opens the way for new cell-based therapies for perio-dontal regeneration. This review provides an overview of adult human stem cells and their potential use in perio-dontal regeneration.

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Faculty Opinions recommendation of Temporal changes in the response of SVZ neural stem cells to intraventricular administration of growth factors.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726121643.793519960 ◽

2016 ◽

Author(s):

Michael Fehlings ◽

Svetlana Altamentova

Keyword(s):

Stem Cells ◽

Growth Factors ◽

Neural Stem Cells ◽

Temporal Changes ◽

Intraventricular Administration

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Biotechnology Applied to Cosmetics and Aesthetic Medicines

Cosmetics ◽

10.3390/cosmetics7020033 ◽

2020 ◽

Vol 7 (2) ◽

pp. 33

Author(s):

Cátia Gomes ◽

Ana Catarina Silva ◽

Ana Camila Marques ◽

José Sousa Lobo ◽

Maria Helena Amaral

Keyword(s):

Stem Cells ◽

Hyaluronic Acid ◽

Growth Factors ◽

Genetic Engineering ◽

Kojic Acid ◽

Active Ingredients ◽

High Quality ◽

Vast Number ◽

Biotechnological Processes

Biotechnology uses microorganisms and/or enzymes to obtain specific products through fermentative processes and/or genetic engineering techniques. Examples of these products are active ingredients, such as hyaluronic acid, kojic acid, resveratrol, and some enzymes, which are used in skin anti-aging products. In addition, certain growth factors, algae, stem cells, and peptides have been included in cosmetics and aesthetic medicines. Thus, biotechnology, cosmetics and aesthetic medicines are now closely linked, through the production of high-quality active ingredients, which are more effective and safer. This work describes the most used active ingredients that are produced from biotechnological processes. Although there are a vast number of active ingredients, the number of biotechnological active ingredients reported in the literature is not significantly high.

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Isolation, Characterization, and Differentiation of Stem Cells From Various Dental Sources: An In Vitro Study

Journal of Advanced Oral Research ◽

10.1177/23202068211010768 ◽

2021 ◽

pp. 232020682110107

Author(s):

Sandeep S. Katti ◽

Kishore Bhat ◽

Chetana Bogar

Keyword(s):

Stem Cells ◽

Growth Factors ◽

Statistical Analysis ◽

Specific Growth ◽

Culture Media ◽

In Vitro Study ◽

Central Research ◽

Cell Lineages ◽

Apical Papilla

Aim: The aim of the current study was to isolate stem cells from various dental sources such as dental pulp, periodontal ligament (PDL), and apical papilla, and to characterize stem cells by staining for the presence/absence of specific surface markers and also to differentiate stem cells into osteogenic, chondrogenic, and adipogenic cell lineages by exposing them to specific growth factors under the ideal conditions. Materials and Methods: A total of 117 samples were included in the study, consisting of 30 pulp, 50 gingival, 35 PDL, and 2 apical papilla samples. The pulp was extirpated and transported to the Central Research Laboratory. Gingival connective tissue was collected from the participants undergoing any crown lengthening procedure or any gingivectomy procedure from the Department of Periodontology. A similar procedure was also followed for apical papilla and PDL. Isolation was done followed by the identification of the cells by immunocytochemistry using different markers. Once the identity of cells was confirmed, these cells were treated with different culture media to attain 70% to 100% confluency. Then the medium was replaced with a conditioning medium containing specific growth factors for differentiation into osteogenic, chondrogenic, and adipogenic cell lineages. Result: In our study, the number of samples collected and processed was 117. The isolation rate of stem cells from the above-collected samples was 70%. Statistical analysis—no statistical analysis was done as there was no variability expected. Conclusion: Our study showed that stem cells could be isolated, differentiated, and characterized from different dental sources.

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Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Biology ◽

10.3390/biology10040317 ◽

2021 ◽

Vol 10 (4) ◽

pp. 317

Author(s):

Eitan Mijiritsky ◽

Haya Drora Assaf ◽

Oren Peleg ◽

Maayan Shacham ◽

Loredana Cerroni ◽

...

Keyword(s):

Growth Factors ◽

Platelet Rich Plasma ◽

Periodontal Regeneration ◽

Clinical Work ◽

Gingival Recession ◽

Vital Role ◽

Facial Rejuvenation ◽

Cochrane Library ◽

The Past ◽

Autologous Platelet

Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.

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Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽

10.3390/ma14102558 ◽

2021 ◽

Vol 14 (10) ◽

pp. 2558

Author(s):

Mihaela Olaru ◽

Liliana Sachelarie ◽

Gabriela Calin

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Growth Factors ◽

Present Review ◽

Modern Concept ◽

Dental Tissues ◽

Tissue Engineering Approach ◽

Hard Dental Tissues ◽

Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

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