scholarly journals Detection of Usutu virus infection in a healthy blood donor from south-west Germany, 2012

2012 ◽  
Vol 17 (50) ◽  
Author(s):  
L Allering ◽  
H Jöst ◽  
P Emmerich ◽  
S Günther ◽  
E Lattwein ◽  
...  
Keyword(s):  
Virus Infection ◽  
Blood Donor ◽  
Blood Donors ◽  
Healthy Blood Donor ◽  
West Germany ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Usutu Virus ◽  
South West

From September 2011 until November 2012, 31 serum samples from German patients with clinically suspected acute Usutu virus (USUV) infections were tested for USUV-specific antibodies. All samples tested negative. In addition, 4,200 serum samples from healthy blood donors from south-west Germany were collected in January 2012 and also analysed for the presence of specific antibodies. One sample tested positive for USUV-IgG and -IgM. Thus, the seroprevalence of USUV antibodies in healthy blood donors from south-west Germany was low in January 2012.

Usutu virus infection in Dutch blood donors

Transfusion ◽  
2019 ◽  
Vol 59 (9) ◽  
pp. 2931-2937 ◽  
Author(s):  
Hans L. Zaaijer ◽  
Ed Slot ◽  
Michel Molier ◽  
Chantal B.E.M. Reusken ◽  
Marco H.G.M. Koppelman
Keyword(s):  
Virus Infection ◽  
Blood Donors ◽  
Usutu Virus

scholarly journals Detection of Specific Antibodies to an Antigenic Mannoprotein for Diagnosis of Penicillium marneffeiPenicilliosis

1998 ◽  
Vol 36 (10) ◽  
pp. 3028-3031 ◽  
Author(s):  
Liang Cao ◽  
Da-Liang Chen ◽  
Cindy Lee ◽  
Che-Man Chan ◽  
King-Man Chan ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Blood Donors ◽  
Antibody Test ◽  
High Specificity ◽  
Pathogenic Fungi ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Immunodeficiency Virus ◽  
Positive Results

The disseminated and progressive fungal disease Penicillium marneffei penicilliosis is one of the most common infectious diseases in AIDS patients in Southeast Asia. To diagnose systemic penicilliosis, we developed an enzyme-linked immunosorbent assay (ELISA)-based antibody test with Mp1p, a purified recombinant antigenic mannoprotein of P. marneffei. Evaluation of the test with guinea pig sera against P. marneffei and other pathogenic fungi indicated that this assay was specific for P. marneffei. Clinical evaluation revealed that high levels of specific antibody were detected in two immunocompetent penicilliosis patients. Furthermore, approximately 80% (14 of 17) of the documented penicilliosis patients with human immunodeficiency virus tested positive for the specific antibody. No false-positive results were found for serum samples from 90 healthy blood donors, 20 patients with typhoid fever, and 55 patients with tuberculosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Mp1p antibody can be of significant value as a diagnostic for penicilliosis.

Serological Evidence of Zika Virus Infection in Febrile Patients and Healthy Blood Donors in Sabah, Malaysian Borneo, 2017–2018

2021 ◽  
Author(s):  
Mya Myat Ngwe Tun ◽  
Daisuke Mori ◽  
Shahnaz Binti Sabri ◽  
Omar Kugan ◽  
Saliz Binti Shaharom ◽  
...  
Keyword(s):  
Zika Virus ◽  
Blood Donors ◽  
Neutralization Test ◽  
Febrile Illness ◽  
Healthy Blood Donor ◽  
Serological Evidence ◽  
Serum Samples ◽  
Zikv Infection ◽  
The Caribbean ◽  
Malaysian Borneo

Several Zika virus (ZIKV) seroprevalence studies have been conducted in Africa, Asia, Oceania, the Americas, and the Caribbean. However, studies on ZIKV seroprevalence are limited in Malaysia though several studies have shown that the disease is endemic in the Malaysian state of Sabah. To evaluate the seroprevalence of ZIKV infection, 818 serum samples were collected from febrile patients and healthy blood donors from the Kudat and Kota Kinabalu districts in Sabah from 2017 to 2018. They were screened for ZIKV infection by IgM and IgG ELISA, and positive ZIKV IgM samples were subjected to a 90% neutralization test for confirmation. Twenty-four (6% [95% CI 4 to 8]) confirmed and two (0.5% [95% CI 0.13 to 1.8]) probable ZIKV infections were detected among 400 febrile illness patients. Of 418 healthy blood donor samples, six (1.4% [95% CI 0.65 to 3]) were determined as confirmed ZIKV infections and six (1.4% [95% CI 0.65 to 3]) indicated probable ZIKV infection. This is the first study on the seroprevalence of ZIKV infections among patients and healthy blood donors in Sabah. Compared with previous studies in Malaysia, this study shows that the incidence of ZIKV infection has increased. It also suggests that a sero-surveillance system is essential to determine the circulation of ZIKV in Sabah, Malaysia.

scholarly journals Prevalence of Iron Deficiency, Iron Deficiency Anaemia and General Anaemia in Male Gambian Blood Donors Residing in Greater Banjul Region

2021 ◽  
Author(s):  
Mustapha Dibbasey ◽  
Bolarinde Lawal ◽  
Solomon Umukoro ◽  
Peter Mitchel
Keyword(s):  
Iron Deficiency ◽  
Blood Donor ◽  
Iron Deficiency Anaemia ◽  
Blood Count ◽  
Blood Donors ◽  
Blood Donation ◽  
Full Blood Count ◽  
Serum Samples ◽  
Deficiency Anaemia ◽  
Donor Population

AbstractObjectiveThe objective of this study is to determine the prevalence of iron deficiency (ID) and iron deficiency anaemia (IDA) as well as general anaemia in male blood donors and their association with ageing process.Methodology and ResultsA total of two hundred and one (201) serum samples were analysed for ferritin in male Gambian blood donors. The ferritin measurement was achieved with COBAS® INTEGRA 400 plus. At the same time, haemoglobin values were retrospectively obtained from the archived haematological full blood count result in the GARIS database. IDA was defined as (Haemoglobin <13.0g/dL+ Ferritin<15ng/ml) whilst ID was defined as (Haemoglobin ≥13.0g/dL+ Ferritin<15ng/ml) and general anaemia was defined as haemoglobin <13.0g/dL in males. The prevalence of anaemia (20%, n=41), ID (22%, n=44) and IDA (10%, n=21), were recorded in male donors. The results show no relationship between ferritin and haemoglobin among the blood donors (collection coefficient (r) = 0.04). Besides, no linear association of having anaemia and ID with ageing was reported among the blood donor population.Conclusion and potential application of findingsID and IDA as well as general anaemia are highly prevalent among blood donors in the Gambia. Besides, no predisposition to ID and anaemia was observed in term of age, thus all blood donors from 18-60 should be considered for blood donation without any age preference.

Investigation on the frequency and clinical course of delta virus infection in south-west Germany

1989 ◽  
Vol 9 ◽  
pp. S115
Author(s):  
H. Berthold ◽  
H.H. Peter ◽  
E. Walter ◽  
C. Heisch ◽  
D. Neumann-Haefelin
Keyword(s):  
Virus Infection ◽  
Clinical Course ◽  
West Germany ◽  
South West ◽  
Delta Virus

scholarly journals IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein

2021 ◽  
Vol 22 (9) ◽  
pp. 4951
Author(s):  
Zorana Lopandić ◽  
Isidora Protić-Rosić ◽  
Aleksandra Todorović ◽  
Sofija Glamočlija ◽  
Marija Gnjatović ◽  
...  
Keyword(s):  
Immune Response ◽  
Virus Infection ◽  
Western Blot ◽  
Host Cells ◽  
M Protein ◽  
Terminal Part ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Antigenic Properties ◽  
Specific Igg

Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.

Absence of the V617F JAK2 Mutation in 181 Healthy Donors.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4664-4664
Author(s):  
Courtney K. Hopkins ◽  
Christian Riley ◽  
Samuel Pepkowitz ◽  
Jean R. Lopategui
Keyword(s):  
Blood Donor ◽  
Mutant Allele ◽  
Blood Donors ◽  
Blood Donation ◽  
Limit Of Detection ◽  
Healthy Blood Donor ◽  
Wild Type Allele ◽  
Wild Type ◽  
Jak2 Mutation ◽  
Donor Population

Abstract INTRODUCTION: Janus kinase 2 gene (JAK2) encodes for a cytoplasmic tyrosine kinase involved in normal hematopoietic growth factor signaling. Point mutations of the JAK2 gene on chromosome 9, specifically V617F, a point mutation at amino acid 617, are associated with myeloproliferative disorders (MPD). The V617F JAK2 mutation has been found in 90% of patients with polycythemia vera, 50–60% of patients with essential thrombocythemia or idiopathic myelofibrosis and 1–5% of patients with other MPD. To our knowledge, previous studies involving the V617F JAK2 mutation were not performed on a control population of normal individuals. Therefore, the prevalence of this mutation has not been established. In this study, we tested volunteer blood donors from a hospital-based blood donation center for the presence of the V617F JAK2 mutation. METHODS: Citrated whole blood was obtained from volunteer blood donors, age 17 and older, who presented to donate whole blood at a hospital-based blood donation center. The donors met all qualifications to donate blood as defined by FDA regulations. DNA was extracted using the QIAagen and QIAamp DNA extraction columns, quantified and diluted to 100ng/ul. DNA was simultaneously amplified and detected using allele specific minor groove binder probes and primers for the V617F JAK2 mutation. The resultant amplification was recorded by real-time, quantitative PCR using an ABI 7500 (Applied Biosystems, Foster City, CA). A 1% limit of detection, determined from sensitivity and specificity studies using a known cell line control, was set as the technically reproducible threshold sensitivity of the test. Samples were defined as negative for the V617F JAK2 mutation if only the wild type allele was detected. Samples that had a mutant allele detected above the 1% limit of detection were defined as positive for the V617F JAK2 mutation. Samples that had a mutant allele detected below the 1% limit of detection were defined as negative for the V617F JAK2 mutation. RESULTS: A total of 181 DNA samples from volunteer blood donors were tested for the V617F JAK2 mutation. The test group consisted of 104 males (mean age 44, range 17–77) and 77 females (mean age 42, range 18–71). Of the 181 donors tested, 171 had only wild type allele detected and were considered negative. Ten donors had high background of the mutant allele detected below the 1% limit of detection and were considered negative. DISCUSSION: To our knowledge, this is the first report documenting the prevalence of the V617F JAK2 mutation in a healthy blood donor population. In this study of 181 volunteer blood donors none had the V617F JAK2 mutation. Although 10 of the 181 donors were found to have mutant allele detected, they were below the 1% technically reproducible sensitivity threshold of the test and were considered negative. We recommend that mutations detected below the technical threshold of 1% of our assay be considered false positives. The results of this study suggest that the V617F JAK2 mutation is not present in a healthy blood donor population and is significant when detected by our method.

scholarly journals Changes in the Whole Blood Donor Population in South-West Germany: 2010 versus 2016

2017 ◽  
Vol 44 (4) ◽  
pp. 217-223 ◽  
Author(s):  
Michael Müller-Steinhardt ◽  
Christian Weidmann ◽  
Harald Klüter
Keyword(s):  
Blood Donor ◽  
Whole Blood ◽  
West Germany ◽  
South West ◽  
Donor Population

scholarly journals A screening for serum markers of arbovirus infections in healthy blood donors from the Volgograd Region

2020 ◽  
Vol 9 (5-6) ◽  
pp. 743-749
Author(s):  
A. О. Negodenkoa ◽  
D. N. Luchinin ◽  
P. Sh. Konovalov ◽  
O. A. Pavlyukova ◽  
E. A. Skrynnikova ◽  
...  
Keyword(s):  
Blood Serum ◽  
Blood Donors ◽  
Clinical Signs ◽  
Serum Samples ◽  
West Nile ◽  
Specific Antibodies ◽  
Age Related ◽  
Tick Borne Encephalitis ◽  
Volgograd Region ◽  
California Serogroup

Volgograd region is considered to be endemic West Nile fever (WNF) area due to the established circulation of the West Nile virus (WNV) therein. Some previous independent studies examining samples collected on the territory of the Volgograd region revealed markers related to the California serogroup (CSG), Sindbis and Ukuniemi viruses. WNF, CSG, Sindbis and Ukuniemi fever mainly being asymptomatic posing thereby a threat of virus spread due to transfusiological manipulations along with vector-borne transmission are manifested by diverse clinical signs. The study was aimed at detection of antibodies specific to West Nile, tick-borne encephalitis, California serogroup (Tyaginya, Inko), Sidbis, and Ukuniemi viruses in blood donors from the Volgograd region. For this, 404 blood sera samples collected from blood donors residing in the Volgograd as well as the Volgograd region were examined by ELISA. It was found that percentage of blood serum samples positive for arbovirus-specific antibodies was 18.32%. Among the, 67 out of 404 (16.58%) samples contained anti-WNV antibodies, 3 out of 279 (1.08%) samples — to tick-borne encephalitis virus, 1 out of 92 (1.09%) — to California serogroup fever and Ukuniemi viruses, and 2 out of 92 (2.17%) — to Sindbis fever virus. Importantly, the peak number of IgG and IgM WNV-positive samples was found among residents of Volgograd (29 out of 110, 26%) and Oktyabrsky district (7 out of 25, 28%). In addition, anti-Sindbis, Ukuniemi and CSG virus antibodies were detected in blood serum samples from blood donors residing in the Kalachevsky region and the city of Volgograd. Analyzing age-related distribution and percentage of seropositive subjects in each age group showed as follows: the lowest percentage (14.5%) was positive for anti-WNV, Sindbis, Ukuniemi and CSG virus antibodies in blood donors aged 32–41 years, whereas the peak percentage (25%) — in the subjects aged 52–61 years. Thus, virus specific antibodies detected in healthy individuals in the aforementioned region evidence about potential recovery after asymptomatic infections. In this regard, further research is required to determine a role of the such arboviruses in the structure of regional infectious diseases. The data obtained suggest a need to continue serological arbovirus monitoring in the Volgograd region.

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