scholarly journals Increase in cases of sorbitol-fermenting E. coli O157:H- in several German states

2002 ◽  
Vol 6 (17) ◽  
Author(s):  
A Ammon ◽  
P Zucs ◽  
C Haas ◽  
F Feil
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
University Hospital ◽  
Escherichia Coli O157 ◽  
German State ◽  
E Coli ◽  
German States ◽  
The University

In the 10 days preceding Easter (19-28 March), four children with haemolytic uraemic syndrome (HUS) and a sibling with diarrhoea were treated at the university hospital of Münster in the German state of North Rhine Westphalia (1). Sorbitol-fermenting Escherichia coli O157:H- was found in faeces of all the cases by the Institute for Hygiene at the university.

scholarly journals Predictors for the development of haemolytic uraemic syndrome with Escherichia coli O157[ratio ]H7 infections: with focus on the day of illness

2000 ◽  
Vol 124 (3) ◽  
pp. 343-349 ◽  
Author(s):  
K. IKEDA ◽  
O. IDA ◽  
K. KIMOTO ◽  
T. TAKATORIGE ◽  
N. NAKANISHI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Primary Schools ◽  
Haemolytic Uraemic Syndrome ◽  
Escherichia Coli O157 ◽  
School Lunches ◽  
E Coli ◽  
Reactive Protein ◽  
Large Outbreak ◽  
Using Data ◽  
Wbc Count

A large outbreak of Escherichia coli O157 infections via school lunches occurred at primary schools in 1996 in Sakai City, Japan. As many as 10000 patients suffered from diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome (HUS). Using data on 288 inpatient school children affected by this outbreak, of whom 36 presented complete HUS and the remaining 252 tested positive for E. coli O157 culture, we attempted to identify predictors for the progression to HUS. Within the first 5 days of illness, clinical features associated with inpatients who developed HUS compared with those without HUS included a C reactive protein (CRP) level higher than 1·2 mg/dl (OR 44·26; 95% CI 5·83–336·23), a white blood cell (WBC) count greater than 11·0 × 109/1 (OR 5·03; 95% CI 27·13–11·87) and a temperature higher than 38·0 °C (OR 5·00; 95% CI 2·25–117·08). It can be concluded that these three factors are predictive factors for the development of HUS in patients with E. coli O157 infection, and patients who have two or all of these factors should be observed closely.

scholarly journals Enhanced surveillance of haemolytic uraemic syndrome and other thrombotic microangiopathies in Scotland, 2003-2004

2005 ◽  
Vol 10 (20) ◽  
Author(s):  
Kevin Pollock
Keyword(s):  
Escherichia Coli ◽  
Renal Failure ◽  
Acute Renal Failure ◽  
Haemolytic Uraemic Syndrome ◽  
Escherichia Coli O157 ◽  
Thrombotic Microangiopathies ◽  
E Coli

Previous surveillance of childhood haemolytic uraemic syndrome (HUS) in Scotland has identified Escherichia coli O157 in over 90% of cases, and infection with E. coli O157 is now reported to be one of the major causes of acute renal failure in children

scholarly journals Serum antibodies to secreted proteins in patients infected with Escherichia coli O157 and other VTEC

1998 ◽  
Vol 120 (3) ◽  
pp. 239-243 ◽  
Author(s):  
H. CHART ◽  
C. JENKINS ◽  
H. R. SMITH ◽  
B. ROWE
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Bacterial Attachment ◽  
Secreted Proteins ◽  
Escherichia Coli O157 ◽  
Serum Antibodies ◽  
E Coli ◽  
Haemorrhagic Colitis ◽  
Additional Information ◽  
Gut Mucosa

Certain strains of verotoxigenic Escherichia coli (VTEC), and in particular those belonging to serogroup O157, cause attaching and effacing (AE) lesions of the host gut mucosa during pathogenesis. The mechanisms involved with bacterial attachment and the destruction of microvilli are determined by a cluster of genes within the LEE region, which also encode five secreted proteins. Sera from patients with antibodies to the lipopolysaccharide (LPS) of E. coli O157 and other VTEC were tested for antibodies to these secreted proteins. Twenty-one of 34 (62%) sera with antibodies to the lipopolysaccharide (LPS) of E. coli O157 also contained antibodies to one or more of the secreted proteins. Five of 12 sera containing antibodies to the LPS of a range of other VTEC serogroups also contained antibodies to 1 or more of the 5 secreted proteins, as did 16 of 70 (23%) sera from patients with haemolytic uraemic syndrome (HUS), haemorrhagic colitis (HC) or diarrhoea, but without bacteriological evidence of infection with VTEC and which did not contain antibodies to VTEC serogroups O5, O115, O145, O153 or O157. The detection of serum antibodies to secreted proteins may provide additional information for interpreting the results of established lipopolysaccharide-based VTEC serology.

scholarly journals Human infections with verocytotoxin-producing Escherichia coli O157 – 10 years of E. coli O157 serodiagnosis

2008 ◽  
Vol 57 (11) ◽  
pp. 1389-1393 ◽  
Author(s):  
Henrik Chart ◽  
Thomas Cheasty
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Enteric Pathogens ◽  
Escherichia Coli O157 ◽  
Health Protection Agency ◽  
Protection Agency ◽  
E Coli ◽  
Males And Females ◽  
Human Infections ◽  
Somatic Antigens

From 1997 to 2007, the Laboratory of Enteric Pathogens (LEP), Health Protection Agency, UK, received sera from 2148 patients for testing for antibodies to the LPS of verocytotoxin-producing Escherichia coli (VTEC) O157. A total of 676 (31.5 %) sera had antibodies binding the LPS of E. coli O157 and the majority of patients were below the age of 10 years, a trend observed for both males and females. Antibody-positive patients had haemolytic uraemic syndrome (HUS) in 79.3 % of cases and most of these presented with the atypical (D−) form of HUS. Nine patients were shown to have antibodies to the LPS of E. coli belonging to serogroups O26 (4), O103 (2), O111 (1) and O145 (2) and one patient had antibodies to the somatic antigens of both E. coli O26 and O103. The serodiagnosis of infections with E. coli O157 and other VTEC continues to be an important adjunct to bacteriology. Where clinicians suspect the involvement of a VTEC in disease, patients' sera should be submitted to the LEP for analysis without delay.

Antibiotic resistance and plasmid pattern of enterotoxigenic ST-a strains of Escherichia coli isolated in Puebla, México

1987 ◽  
Vol 33 (9) ◽  
pp. 816-819 ◽  
Author(s):  
L. Y. Martínez ◽  
M. M. P. Arenas ◽  
M. Y. R. Montes ◽  
L. J. Martínez ◽  
B. E. Baca
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Selective Pressure ◽  
University Hospital ◽  
Gene Encoding ◽  
E Coli ◽  
Heat Stable ◽  
Antibiotic Susceptibilities ◽  
Almost All ◽  
The University

Antibiotic susceptibilities of 22 strains of Escherichia coli isolated from children from 0 to 3 years old at the University Hospital of Puebla were determined. Almost all strains were resistant to ampicillin, tetracycline, streptomycin, and kanamycin. Gel electrophoresis of DNA from 10 clinical strains of E. coli revealed a heterogeneous plasmid population. Plasmid DNA, ranging in molecular mass from 1.8 to 120 megadaltons, was demonstrated in 10 strains. Moreover, the frequency of antibiotic transfer ranged from 1.6/108 to 2/10, and the simultaneous transfer of the gene encoding heat-stable enterotoxin was also determined. Six out of 10 strains tested were able to cotransfer ST-a as demonstrated by the suckling mouse test. It is possible that antibiotic selective pressure may increase the isolation of enterotoxigenic E. coli strains.

scholarly journals Epidemiology of infection due to Escherichia coli O157: a 3-year prospective study

1996 ◽  
Vol 116 (3) ◽  
pp. 279-284 ◽  
Author(s):  
I. A. R. MacDonald ◽  
I. M. Gould ◽  
J. Curnow
Keyword(s):  
Escherichia Coli ◽  
Water Supply ◽  
Prospective Study ◽  
Haemolytic Uraemic Syndrome ◽  
Acute Diarrhoea ◽  
Risk Groups ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Agricultural Community ◽  
Case Transmission

SUMMARYA 3-year study of Escherichia coli infections in Grampian Region was conducted to ascertain the incidence, document clinical sequelae and identify at-risk groups. Approximately 30000 stools from patients with acute diarrhoea were screened for E. coli O157, and an epidemiological questionnaire filled in for each patient whose stool was positive. Eighty-three patients were studied. The annual incidence was 6 per 100000. Proportionately more infections occurred in people involved in agriculture. Evidence was seen of case-to-case transmission, and contamination of a water supply. Eight cases developed haemolytic uraemic syndrome (HUS). There were 2 deaths due to HUS and 2 due to haemorrhagic colitis (HC). Symptomatic E. coli infection is relatively common in the Grampian Region, more common in the agricultural community, and is the main cause of HUS in this Region.

scholarly journals HUS due to a sorbitol-fermenting verotoxigenic E. coli O157 in Scotland

2002 ◽  
Vol 6 (44) ◽  
Author(s):  
L Allison
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Reference Laboratory ◽  
Escherichia Coli O157 ◽  
O157 Strain ◽  
E Coli ◽  
Source Of Infection

The Scottish Escherichia coli O157 Reference Laboratory (SERL) has reported the isolation of a sorbitol-fermenting verotoxigenic E. coli (VTEC) O157 strain from a 5 year old child with haemolytic uraemic syndrome (HUS) (1). No source of infection has yet been identified.

Antimicrobial Effects of Zataria multiflora and Ocimum basilicum on Escherichia coli O157:H7 During Ripening of Traditional Lighvan Cheese

2020 ◽  
Vol 16 (3) ◽  
pp. 373-380
Author(s):  
Mohammad B. Zendeh ◽  
Vadood Razavilar ◽  
Hamid Mirzaei ◽  
Khosrow Mohammadi
Keyword(s):  
Escherichia Coli ◽  
Essential Oils ◽  
Dairy Products ◽  
Antimicrobial Agents ◽  
Escherichia Coli O157 ◽  
Zataria Multiflora ◽  
Antimicrobial Effects ◽  
E Coli ◽  
Common Causes ◽  
Lighvan Cheese

Background: Escherichia coli O157:H7 is one of the most common causes of contamination in Lighvan cheese processing. Using from natural antimicrobial essential oils is applied method to decrease the rate of microbial contamination of dairy products. The present investigation was done to study the antimicrobial effects of Z. multiflora and O. basilicum essential oils on survival of E. coli O157:H7 during ripening of traditional Lighvan cheese. Methods: Leaves of the Z. multiflora and O. basilicum plants were subjected to the Clevenger apparatus. Concentrations of 0, 100 and 200 ppm of the Z. multiflora and 0, 50 and 100 ppm of O. basilicum essential oils and also 103 and 105 cfu/ml numbers of E. coli O157:H7 were used. The numbers of the E. coli O157:H7 bacteria were analyzed during the days 0, 30, 60 and 90 of the ripening period. Results: Z. multiflora and O. basilicum essential oils had considerable antimicrobial effects against E. coli O157:H7. Using the essential oils caused decrease in the numbers of E. coli O157:H7 bacteria in 90th days of ripening (P <0.05). Using from Z. multiflora at concentration of 200 ppm can reduce the survival of E. coli O157:H7 in Lighvan cheese. Conclusion: Using Z. multiflora and O. basilicum essential oils as good antimicrobial agents can reduce the risk of foodborne bacteria and especially E. coli O157:H7 in food products.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep

2009 ◽  
Vol 89 (2) ◽  
pp. 285-293 ◽  
Author(s):  
S J Bach ◽  
R P Johnson ◽  
K. Stanford ◽  
T A McAllister
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Total Coliforms ◽  
E Coli ◽  
Oral Inoculation ◽  
Experimental Treatments ◽  
And Control

Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages (phage), and control (CON). Oral inoculation of the rams with 109 CFU of a mixture of four nalidixic acid-resistant strains of E. coli O157:H7 was performed on day 0. A mixture of 1010 PFU of bacteriophages P5, P8 and P11 was administered on days -2, -1, 0, 6 and 7. Fecal samples collected on 14 occasions over 21 d were analyzed for E. coli O157:H7, total E. coli, total coliforms and bacteriophages. Sheep in treatment O157+phage shed fewer (P < 0.05) E. coli O157:H7 than did sheep in treatment O157. Populations of total coliforms and total E. coli were similar (P < 0.05) among treatments, implying that bacteriophage lysis of non-target E. coli and coliform bacteria in the gastrointestinal tract did not occur. Bacteriophage numbers declined rapidly over 21 d, which likely reduced the chance of collision between bacteria and bacteriophage. Oral administration of bacteriophages reduced shedding of E. coli O157:H7 by sheep, but a delivery system that would protect bacteriophages during passage through the intestine may increase the effectiveness of this strategy as well as allow phage to be administered in the feed.Key words: Escherichia coli O157:H7, bacteriophage, sheep, environment, coliforms

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