scholarly journals Human infections with verocytotoxin-producing Escherichia coli O157 – 10 years of E. coli O157 serodiagnosis

2008 ◽  
Vol 57 (11) ◽  
pp. 1389-1393 ◽  
Author(s):  
Henrik Chart ◽  
Thomas Cheasty
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Enteric Pathogens ◽  
Escherichia Coli O157 ◽  
Health Protection Agency ◽  
Protection Agency ◽  
E Coli ◽  
Males And Females ◽  
Human Infections ◽  
Somatic Antigens

From 1997 to 2007, the Laboratory of Enteric Pathogens (LEP), Health Protection Agency, UK, received sera from 2148 patients for testing for antibodies to the LPS of verocytotoxin-producing Escherichia coli (VTEC) O157. A total of 676 (31.5 %) sera had antibodies binding the LPS of E. coli O157 and the majority of patients were below the age of 10 years, a trend observed for both males and females. Antibody-positive patients had haemolytic uraemic syndrome (HUS) in 79.3 % of cases and most of these presented with the atypical (D−) form of HUS. Nine patients were shown to have antibodies to the LPS of E. coli belonging to serogroups O26 (4), O103 (2), O111 (1) and O145 (2) and one patient had antibodies to the somatic antigens of both E. coli O26 and O103. The serodiagnosis of infections with E. coli O157 and other VTEC continues to be an important adjunct to bacteriology. Where clinicians suspect the involvement of a VTEC in disease, patients' sera should be submitted to the LEP for analysis without delay.

scholarly journals Effect of Cattle Diet on Escherichia coli O157:H7 Acid Resistance

1999 ◽  
Vol 65 (7) ◽  
pp. 3233-3235 ◽  
Author(s):  
Carolyn J. Hovde ◽  
Paula R. Austin ◽  
Karen A. Cloud ◽  
Christopher J. Williams ◽  
Carl W. Hunt
Keyword(s):  
Escherichia Coli ◽  
Acid Resistance ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Human Infections

ABSTRACT The duration of shedding of Escherichia coli O157 isolates by hay-fed and grain-fed steers experimentally inoculated withE. coli O157:H7 was compared, as well as the acid resistance of the bacteria. The hay-fed animals shed E. coli O157 longer than the grain-fed animals, and irrespective of diet, these bacteria were equally acid resistant. Feeding cattle hay may increase human infections with E. coli O157:H7.

scholarly journals Fecal Metagenome Sequences from Lactating Dairy Cows Shedding Escherichia coli O157:H7

2018 ◽  
Vol 7 (18) ◽  
Author(s):  
Serajus Salaheen ◽  
Seon Woo Kim ◽  
Jeffrey S. Karns ◽  
Bradd J. Haley ◽  
Jo Ann S. Van Kessel
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Causative Agent ◽  
Public Domain ◽  
Escherichia Coli O157 ◽  
Content Type ◽  
The Public ◽  
E Coli ◽  
Lactating Dairy Cows ◽  
Human Infections

Cattle are primary reservoirs of Escherichia coli O157:H7, a causative agent of severe human infections. To facilitate analyses of the communities in which this pathogen is found, we sequenced the fecal metagenomes of 10 dairy cows shedding E. coli O157:H7 and added them to the public domain.

Specific Identification of Escherichia coli O157 by an Immunostick Method Using Commercially Available Antibodies

1996 ◽  
Vol 59 (6) ◽  
pp. 670-673 ◽  
Author(s):  
SHIU W. HUANG ◽  
TSUNG C. CHANG
Keyword(s):  
Escherichia Coli ◽  
Rapid Identification ◽  
Escherichia Coli O157 ◽  
Commercial Preparation ◽  
E Coli ◽  
Specificity And Sensitivity ◽  
Sandwich Enzyme Immunoassay ◽  
Antigen Antibody ◽  
Phosphate Buffered Saline ◽  
Somatic Antigens

A sandwich enzyme-immunoassay performed on plastic sticks was developed to specifically identify Escherichia coli O157. Colonies of test bacteria grown on tryptic soy agar were suspended in phosphate-buffered saline, heated in a 100°C water bath for 15 min, and incubated with the plastic sticks coated with a commercial preparation of anti-E. coli O157 antibodies at 37°C for 1.5 h. After incubation, the same antibodies labeled with peroxidase were used to produce the signal of antigen-antibody reaction. For 35 strains of E. coli O157 (among them 34 were E. coli O157:H7) tested, all produced strong reactions by the immunoassay. For 162 strains of E. coli with somatic antigens other than O157 and 38 strains of other genera tested, only one strain (Salmonella bietri) produced a false-positive reaction. The specificity and sensitivity of the immunostick assay were 100% (35/35) and 99.5% (199/200), respectively. The detection limit of the assay for E. coli O157:H7 (CCRC 15991) was about 105 CFU/ml. The method, which can be carried out within 3 h, is useful for rapid identification of suspect E. coli O157 isolated on selective media.

scholarly journals Predictors for the development of haemolytic uraemic syndrome with Escherichia coli O157[ratio ]H7 infections: with focus on the day of illness

2000 ◽  
Vol 124 (3) ◽  
pp. 343-349 ◽  
Author(s):  
K. IKEDA ◽  
O. IDA ◽  
K. KIMOTO ◽  
T. TAKATORIGE ◽  
N. NAKANISHI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Primary Schools ◽  
Haemolytic Uraemic Syndrome ◽  
Escherichia Coli O157 ◽  
School Lunches ◽  
E Coli ◽  
Reactive Protein ◽  
Large Outbreak ◽  
Using Data ◽  
Wbc Count

A large outbreak of Escherichia coli O157 infections via school lunches occurred at primary schools in 1996 in Sakai City, Japan. As many as 10000 patients suffered from diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome (HUS). Using data on 288 inpatient school children affected by this outbreak, of whom 36 presented complete HUS and the remaining 252 tested positive for E. coli O157 culture, we attempted to identify predictors for the progression to HUS. Within the first 5 days of illness, clinical features associated with inpatients who developed HUS compared with those without HUS included a C reactive protein (CRP) level higher than 1·2 mg/dl (OR 44·26; 95% CI 5·83–336·23), a white blood cell (WBC) count greater than 11·0 × 109/1 (OR 5·03; 95% CI 27·13–11·87) and a temperature higher than 38·0 °C (OR 5·00; 95% CI 2·25–117·08). It can be concluded that these three factors are predictive factors for the development of HUS in patients with E. coli O157 infection, and patients who have two or all of these factors should be observed closely.

scholarly journals Enhanced surveillance of haemolytic uraemic syndrome and other thrombotic microangiopathies in Scotland, 2003-2004

2005 ◽  
Vol 10 (20) ◽  
Author(s):  
Kevin Pollock
Keyword(s):  
Escherichia Coli ◽  
Renal Failure ◽  
Acute Renal Failure ◽  
Haemolytic Uraemic Syndrome ◽  
Escherichia Coli O157 ◽  
Thrombotic Microangiopathies ◽  
E Coli

Previous surveillance of childhood haemolytic uraemic syndrome (HUS) in Scotland has identified Escherichia coli O157 in over 90% of cases, and infection with E. coli O157 is now reported to be one of the major causes of acute renal failure in children

Escherichia coli O157:H7 as the Predominant Pathogen Associated With the Hemolytic Uremic Syndrome: A Prospective Study in the Pacific Northwest

PEDIATRICS ◽  
1987 ◽  
Vol 80 (1) ◽  
pp. 37-40
Author(s):  
Marguerite A. Neill ◽  
Phillip I. Tarr ◽  
Carla R. Clausen ◽  
Dennis L. Christie ◽  
Robert O. Hickman
Keyword(s):  
Escherichia Coli ◽  
Prospective Study ◽  
Hemolytic Uremic Syndrome ◽  
Pacific Northwest ◽  
Enteric Pathogens ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
The Pacific ◽  
Uremic Syndrome ◽  
A Prospective Study

During a 12-month period, 14 patients with the hemolytic uremic syndrome were identified in a prospective study of enteric pathogens associated with this disorder. Of the 12 patients with a diarrheal illness preceding the onset of hemolytic uremic syndrome, fecal Escherichia coli O157:H7 was detected in seven (58%), all of whom had bloody diarrhea. Half of the siblings of these patients had concurrent nonbloody diarrhea. No source for infection with this organism was identified. Enteric infection with E coli O157:H7 occurs in the majority of cases of hemolytic uremic syndrome following diarrheal illness in the Pacific Northwest and may represent a previously overlooked cause of hemolytic uremic syndrome in other locales. Evaluation of all cases of hemolytic uremic syndrome for enteric pathogens should routinely include cultures for E coli O157:H7 until results of additional studies clarify the distribution of agents associated with hemolytic uremic syndrome in different geographic regions. These findings may provide new opportunities for the design of therapeutic and preventive strategies in this disorder.

scholarly journals Vero cytotoxin-producingEscherichia coli, particularly serogroup O157, associated with human infections in England and Wales: 1992–4

1996 ◽  
Vol 117 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A. Thomas ◽  
T. Cheasty ◽  
J. A. Frost ◽  
H. Chart ◽  
H. R. Smith ◽  
...  
Keyword(s):  
Haemolytic Uraemic Syndrome ◽  
Enteric Pathogens ◽  
Bacterial Isolates ◽  
Average Annual Rate ◽  
England And Wales ◽  
E Coli ◽  
Phage Types ◽  
Human Infections ◽  
Gene Probing ◽  
Cytotoxin Gene

SummaryInvestigations were performed by the Laboratory of Enteric Pathogens on Vero cytotoxin-producingEscherichia coli(VTEC) in England and Wales from 1992–4. Bacterial isolates, faeces and sera obtained from patients with diarrhoea, bloody diarrhoea and haemolytic uraemic syndrome were examined. Using serotyping, Vero cytotoxin gene probing and serodiagnostic tests forE. coliO157, evidence of infection was detected in 543, 434 and 491 individuals in 1992, 1993 and 1994 respectively; VTEC of serogroup O157 were isolated from 470, 385 and 411 cases. The O157 VTEC strains belonged to at least 19 different phage types (PT) although 84% belonged to PT2, PT49, PT8, PT1 or PT4. Antibodies toE. coliO157 lipopolysaccharide were detected in 13% of the cases. The average annual rate of infection with O157 VTEC was 0·83/100000 and 12% of the 1458 individuals with evidence of infection with VTEC orE. coliO157 developed haemolytic uraemic syndrome. There were at least 18 general outbreaks and many family outbreaks.

scholarly journals Serum antibodies to secreted proteins in patients infected with Escherichia coli O157 and other VTEC

1998 ◽  
Vol 120 (3) ◽  
pp. 239-243 ◽  
Author(s):  
H. CHART ◽  
C. JENKINS ◽  
H. R. SMITH ◽  
B. ROWE
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
Bacterial Attachment ◽  
Secreted Proteins ◽  
Escherichia Coli O157 ◽  
Serum Antibodies ◽  
E Coli ◽  
Haemorrhagic Colitis ◽  
Additional Information ◽  
Gut Mucosa

Certain strains of verotoxigenic Escherichia coli (VTEC), and in particular those belonging to serogroup O157, cause attaching and effacing (AE) lesions of the host gut mucosa during pathogenesis. The mechanisms involved with bacterial attachment and the destruction of microvilli are determined by a cluster of genes within the LEE region, which also encode five secreted proteins. Sera from patients with antibodies to the lipopolysaccharide (LPS) of E. coli O157 and other VTEC were tested for antibodies to these secreted proteins. Twenty-one of 34 (62%) sera with antibodies to the lipopolysaccharide (LPS) of E. coli O157 also contained antibodies to one or more of the secreted proteins. Five of 12 sera containing antibodies to the LPS of a range of other VTEC serogroups also contained antibodies to 1 or more of the 5 secreted proteins, as did 16 of 70 (23%) sera from patients with haemolytic uraemic syndrome (HUS), haemorrhagic colitis (HC) or diarrhoea, but without bacteriological evidence of infection with VTEC and which did not contain antibodies to VTEC serogroups O5, O115, O145, O153 or O157. The detection of serum antibodies to secreted proteins may provide additional information for interpreting the results of established lipopolysaccharide-based VTEC serology.

scholarly journals Indirect Transmission of Escherichia coli O157:H7 Occurs Readily among Swine but Not among Sheep

2008 ◽  
Vol 74 (8) ◽  
pp. 2488-2491 ◽  
Author(s):  
Nancy A. Cornick ◽  
Hung VuKhac
Keyword(s):  
Escherichia Coli ◽  
Direct Contact ◽  
Infected Animal ◽  
Swine Manure ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Indirect Transmission ◽  
Pork Products ◽  
Infected Donor ◽  
Human Infections

ABSTRACT Transmission of Escherichia coli O157:H7 among reservoir animals is generally thought to occur either by direct contact between a naïve animal and an infected animal or by consumption of food or water containing the organism. Although ruminants are considered the major reservoir, there are two reports of human infections caused by E. coli O157:H7 linked to the consumption of pork products or to the contamination of fresh produce by swine manure. The objective of this study was to determine whether E. coli O157:H7 could be transmitted to naïve animals, both sheep and swine, that did not have any direct contact with an infected donor animal. We recovered E. coli O157:H7 from 10/10 pigs with nose-to-nose contact with the infected donor or animals adjacent to the donor and from 5/6 naïve pigs that were penned in the same room as the donor pig but 10 to 20 ft away. In contrast, when the experiment was repeated with sheep, E. coli O157:H7 was recovered from 4/6 animals that had nose-to-nose contact with the infected donor or adjacent animals and from 0/6 naïve animals penned 10 to 20 ft away from the donor. These results suggest that E. coli O157:H7 is readily transmitted among swine and that transmission can occur by the creation of contaminated aerosols.

scholarly journals Increase in cases of sorbitol-fermenting E. coli O157:H- in several German states

2002 ◽  
Vol 6 (17) ◽  
Author(s):  
A Ammon ◽  
P Zucs ◽  
C Haas ◽  
F Feil
Keyword(s):  
Escherichia Coli ◽  
Haemolytic Uraemic Syndrome ◽  
University Hospital ◽  
Escherichia Coli O157 ◽  
German State ◽  
E Coli ◽  
German States ◽  
The University

In the 10 days preceding Easter (19-28 March), four children with haemolytic uraemic syndrome (HUS) and a sibling with diarrhoea were treated at the university hospital of Münster in the German state of North Rhine Westphalia (1). Sorbitol-fermenting Escherichia coli O157:H- was found in faeces of all the cases by the Institute for Hygiene at the university.

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