scholarly journals Growth of tobacco hairy roots with constitutive expression of the NtEXPA5 expansin gene

2020 ◽  
Author(s):  
B. R. Kuluev ◽  
H. G. Musin ◽  
G. R. Gumerova ◽  
A. V. Chemeris
Keyword(s):  
Hairy Roots ◽  
Growth Rates ◽  
Constitutive Expression ◽  
Stress Factors ◽  
Expansin Gene ◽  
Tobacco Hairy Roots

Tobacco hairy roots with constitutive expression of the NtEXPA5 expansin gene were obtained and analyzed, which were characterized by higher growth rates and increased productivity under normal conditions, as well as the action of stress factors.

Root growth of transgenic tobacco plants with constitutive expression of the expansin gene PnEXPA3 under the action of stress factors.

Biomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 545-551
Author(s):  
Z.A. Berezhneva ◽  
B.R. Kuluev
Keyword(s):  
Heavy Metals ◽  
Transgenic Tobacco ◽  
Constitutive Expression ◽  
Stress Factors ◽  
Cultivated Plants ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants ◽  
Expansin Gene ◽  
Agricultural Plants

Plant α-expansin genes are of great interest for genetic engineering, since they can be used to improve the growth of the roots of agricultural plants. Previously, we obtained transgenic tobacco plants Nicotiana tabacum overexpressing the PnEXPA3 expansin gene from black poplar Populus nigra. In this work, the growth of roots of transgenic plants was studied under normal conditions and under the influence of stress factors such as hypothermia, salinity and heavy metals. Constitutive expression of the PnEXPA3 gene improved the growth of roots of transgenic tobacco plants as compared to the wild type under normal conditions and under hypothermia (+10°C), salinity (50 and 100 mM NaCl) and the action of heavy metals (200 and 400 μM acetate cadmium). The totality of the obtained data suggests that the constitutive expression of the PnEXPA3 gene can be used to improve the growth of roots of cultivated plants both under normal conditions and the action of stress factors.

Features of growth of hairy roots of tobacco with constitutive expression of NtEXPA5 expansin gene

Biomics ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 378-385
Author(s):  
K.G. Musin ◽  
E.R. Serbaeva ◽  
V.V. Fedyaev ◽  
G.R. Gumerova ◽  
B.R. Kuluev
Keyword(s):  
Hairy Roots ◽  
Constitutive Expression ◽  
Expansin Gene

Obtaining of hairy roots cultures of kok-saghyz and analysis of the content of natural rubber

Biomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 449-454
Author(s):  
B.R. Kuluev ◽  
G.R. Gumerova ◽  
A.V. Knyazev ◽  
Kh.G. Musin ◽  
A.V. Chemeris
Keyword(s):  
Natural Rubber ◽  
Hairy Roots ◽  
Industrial Production ◽  
Hevea Brasiliensis ◽  
Dry Weight ◽  
Hexane Extract ◽  
Raw Material ◽  
Stress Factors ◽  
Special Equipment ◽  
High Quality

Kok-saghyz (Taraxacum kok-saghyz Rodin) is an alternative to Hevea brasiliensis source of high quality natural rubber that is able to grow in temperate climates. However, the field cultivation of kok-saghyz is associated with a number of problems, such as the need for seed stratification, seedlings that are very sensitive to stress factors, laboriousness in the fight against weeds and pests, uneven maturation of seeds, the lack of special equipment for sowing, collecting roots and seeds. In this regard, the use of hairy roots of kok-saghyz as a raw material for obtaining natural rubber seems to be promising. The aim of our work was to obtain cultures of hairy roots of kok-saghyz and to determine the content of natural rubber in them by the hexane method. In the course of the work, 10 lines of hairy roots of kok-saghyz were obtained, capable of growing in isolated cultures. It has been shown that these hairy roots accumulate an average of 7.5% hexane extract (rubber-like substances) per dry weight, which is about 1.5 times more than the roots of field kok-saghyz. Our data indicate that the use of the hairy roots of kok-saghyz is promising for the industrial production of natural rubber.

Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody

2013 ◽  
Vol 111 (2) ◽  
pp. 336-346 ◽  
Author(s):  
Suvi T. Häkkinen ◽  
Nicole Raven ◽  
Maurice Henquet ◽  
Marja-Leena Laukkanen ◽  
Tibor Anderlei ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Molecular Farming ◽  
Tobacco Hairy Roots

Root-preferential expression of Newcastle virus glycoproteins driven by NtREL1 promoter in tobacco hairy roots and evaluation of oral delivery in mice

2022 ◽  
Author(s):  
Narges Arkian Boroujeni ◽  
Somayeh Behjat Khatouni ◽  
Mohammad Javad Motamedi ◽  
Shaghayegh Afraz ◽  
Mahyat Jafari ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Oral Delivery ◽  
Preferential Expression ◽  
Tobacco Hairy Roots

Production of monoclonal antibodies by tobacco hairy roots

1997 ◽  
Vol 54 (5) ◽  
pp. 401-415 ◽  
Author(s):  
Raviwan Wongsamuth ◽  
Pauline M. Doran
Keyword(s):  
Monoclonal Antibodies ◽  
Hairy Roots ◽  
Tobacco Hairy Roots

scholarly journals Revisiting anabasine biosynthesis in tobacco hairy roots expressing plant lysine decarboxylase gene by using 15N-labeled lysine

2014 ◽  
Vol 31 (5) ◽  
pp. 511-518 ◽  
Author(s):  
Somnuk Bunsupa ◽  
Kana Komastsu ◽  
Ryo Nakabayashi ◽  
Kazuki Saito ◽  
Mami Yamazaki
Keyword(s):  
Hairy Roots ◽  
Lysine Decarboxylase ◽  
Tobacco Hairy Roots
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