In Vitro Effect of Biofield Energy Treated DMEM On Mitochondrial Biogenesis Using Myoblasts Cell Line, C2C12

There is evidence in rodents to suggest that theacrine-based supplements modulate tissue sirtuin activity as well as other biological processes associated with aging. Herein, we examined if a theacrine-based supplement (termed NAD3) altered sirtuin activity in vitro while also affecting markers of mitochondrial biogenesis. The murine C2C12 myoblast cell line was used for experimentation. Following 7 days of differentiation, myotubes were treated with 0.45 mg/mL of NAD3 (containing ~2 mM theacrine) for 3 and 24 h (n = 6 treatment wells per time point). Relative to control (CTL)-treated cells, NAD3 treatments increased (p < 0.05) Sirt1 mRNA levels at 3 h, as well as global sirtuin activity at 3 and 24 h. Follow-up experiments comparing 24 h NAD3 or CTL treatments indicated that NAD3 increased nicotinamide phosphoribosyltransferase (NAMPT) and SIRT1 protein levels (p < 0.05). Cellular nicotinamide adenine dinucleotide (NAD+) levels were also elevated nearly two-fold after 24 h of NAD3 versus CTL treatments (p < 0.001). Markers of mitochondrial biogenesis were minimally affected. Although these data are limited to select biomarkers in vitro, these preliminary findings suggest that a theacrine-based supplement can modulate select biomarkers related to NAD+ biogenesis and sirtuin activity. However, these changes did not drive increases in mitochondrial biogenesis. While promising, these data are limited to a rodent cell line and human muscle biopsy studies are needed to validate and elucidate the significance of these findings.

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In Vitro Effect Of VPA Together With ATRA and Ara-C On Proliferation and Viability Of Pediatric AML Cell Line THP-1

Blood ◽

10.1182/blood.v122.21.4924.4924 ◽

2013 ◽

Vol 122 (21) ◽

pp. 4924-4924

Author(s):

Sema Anak ◽

Ayca Gul Kanbay ◽

Cagri Gulec

Keyword(s):

Cell Line ◽

Animal Studies ◽

Conflicts Of Interest ◽

Bipolar Disorders ◽

Mood Stabilizer ◽

Leukemic Cells ◽

Pcr Method ◽

Pediatric Aml ◽

Vitro Effect

Abstract In addition to its HDAC inhibitory property, Valproic acid is also known as anticonvulsant agent and mood stabilizer in the treatment of bipolar disorders. Due to its HDAC inhibitory activity and its safety in long-term usage, VPA is presumed to be a good candidate for cancer treatment. It is known that VPA induces apoptosis in leukemic cells, while not in normal cells. VPA is reported as an effective agent in treatment of pediatric AML in clinical studies and is also well tolerated in children. In this study, the in vitro effect of the combination of HDAC inhibitor VPA with Ara-C and ATRA which are used in AML therapy, is investigated on AML cells. For this purpose, the effect of VPA, Ara-C and ATRA on proliferation of AML cell line THP-1 is tested in cell culture condition. To assess the effect on cell proliferation, p21 expression was measured by RT-PCR method. The use of VPA alone, did not affect the cell viability, while increasing the expression of the p21 gene. VPA in combination with Ara-C, increased the expression of p21 gene more than the other combinations. Thus it is determined that the p21 gene expression is higher as a result of known cell cycle stops. In this study, the understanding of how effective is VPA together with ATRA and/or Ara-C on AML cells, might be a good start for animal studies and clinical trials as a remarkable data for the development of new chemotherapeutic protocols. Disclosures: No relevant conflicts of interest to declare.

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Synthesis, Characterization and In Vitro Effect of Doxorubicin-Loaded Folate-PEG-PNIPAAm on Human Hepatocellular Cell Line

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2018.2792 ◽

2018 ◽

Vol 10 (10) ◽

pp. 1398-1404

Author(s):

Zhulin Wu ◽

Chensheng Ouyang ◽

Li He ◽

Anan Wangli ◽

Shasha Pan ◽

...

Keyword(s):

Cell Line ◽

Vitro Effect

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Ghrelin and cortistatin in lung cancer: Expression of peptides and related receptors in human primary tumors and in vitro effect on the H345 small cell carcinoma cell line

Journal of Endocrinological Investigation ◽

10.1007/bf03347371 ◽

2006 ◽

Vol 29 (9) ◽

pp. 781-790 ◽

Cited By ~ 36

Author(s):

P. Cassoni ◽

E. Allia ◽

T. Marrocco ◽

C. Ghè ◽

E. Ghigo ◽

...

Keyword(s):

Lung Cancer ◽

Cell Carcinoma ◽

Cell Line ◽

Small Cell Carcinoma ◽

Carcinoma Cell ◽

Small Cell ◽

Carcinoma Cell Line ◽

Primary Tumors ◽

Vitro Effect

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Suppressive Effect of Fig (Ficus Carica) Latex on Esophageal Cancer Cell Proliferation

Acta Facultatis Medicae Naissensis ◽

10.2478/v10283-012-0042-2 ◽

2013 ◽

Vol 30 (2) ◽

pp. 93-96 ◽

Cited By ~ 4

Author(s):

Seyyed Abbas Hashemi ◽

Saeid Abediankenari

Keyword(s):

Esophageal Cancer ◽

Cell Line ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Line ◽

Ficus Carica ◽

Esophageal Cancer Cell ◽

Fig Tree ◽

Vitro Effect

SUMMARY Fig (Ficus carica) tree latex was a source of treatment of different diseases in the Iranian traditional medicine reported by Avicenna in his 10th century book Canon of Medicine. The aim of this investigation was to establish the anticancer effect of fig tree latex on human cancer cells. The in vitro effect of different doses of fig tree latex including 2.5 mg/ml, 5 mg/ml, and 10 mg/ml on esophageal cancer cell line was evaluated after 72 hours by MTT assay. There was a significant change in 10 mg/ml treatment of latex after 72 hours on esophageal cancer line (P; 0.025). Ten mg/ml was the optimum concentration in the inhibition of cell line growth. Fig (Ficus carica) tree latex could be a candidate as a potential agent for the inhibition of cancerous cells production and development.

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Synthesis of isoxazoline-linked chlorins and their in vitro cell viabilities

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424610002690 ◽

2010 ◽

Vol 14 (10) ◽

pp. 859-865 ◽

Cited By ~ 5

Author(s):

Jin-Jun Wang ◽

Jia-Zhu Li ◽

Yun-Wei Li ◽

Judit Jakus ◽

Young Key Shim

Keyword(s):

Photodynamic Therapy ◽

Cell Line ◽

Starting Material ◽

Nitrile Oxide ◽

Dipolar Cycloaddition ◽

Mouse Sarcoma ◽

Pyropheophorbide A ◽

Vinyl Group ◽

Vitro Effect

A concise synthesis of isoxazoline-linked chlorins is described. This approach is carried out from methyl pyropheophorbide-a as the starting material via 1,3-dipolar cycloaddition of a vinyl group on the periphery with nitrile oxide to give regioselective products with excellent yields. This method represents an extensive and efficient entry into the functionalization of chlorins with a chlorophyll-α skeleton. Moreover, we have examined a preliminary in vitro effect of these new derivatives on mouse sarcoma S-180 cell line in photodynamic therapy.

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In vitro effect of oligo-hydroxyalkanoates on the growth of mouse fibroblast cell line L929

Biomaterials ◽

10.1016/j.biomaterials.2007.05.011 ◽

2007 ◽

Vol 28 (27) ◽

pp. 3896-3903 ◽

Cited By ~ 79

Author(s):

J SUN ◽

Z DAI ◽

Y ZHAO ◽

G CHEN

Keyword(s):

Cell Line ◽

Fibroblast Cell ◽

Mouse Fibroblast ◽

Fibroblast Cell Line ◽

Mouse Fibroblast Cell ◽

Mouse Fibroblast Cell Line ◽

Vitro Effect

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Synthesis, Characterization, In Vitro Anticancer Potentiality, and Antimicrobial Activities of Novel Peptide–Glycyrrhetinic-Acid-Based Derivatives

Molecules ◽

10.3390/molecules26154573 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4573

Author(s):

Gaber O. Moustafa ◽

Ahmed Shalaby ◽

Ahmed M. Naglah ◽

Marwa M. Mounier ◽

Heba El-Sayed ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Line ◽

Cancer Cell ◽

Micrococcus Luteus ◽

Normal Skin ◽

Cancer Cell Line ◽

Glycyrrhetinic Acid ◽

Pentacyclic Triterpenoids ◽

Vitro Effect

Glycyrrhetinic acid (GA) is one of many interesting pentacyclic triterpenoids showing significant anticancer activity by triggering apoptosis in tumor cell lines. This study deals with the design and synthesis of new glycyrrhetinic acid (GA)–amino acid peptides and peptide ester derivatives. The structures of the new derivatives were established through various spectral and microanalytical data. The novel compounds were screened for their in vitro cytotoxic activity. The evaluation results showed that the new peptides produced promising cytotoxic activity against the human breast MCF-7 cancer cell line while comparing to doxorubicin. On the other hand, only compounds 3, 5, and 7 produced potent activity against human colon HCT-116 cancer cell line. The human liver cancer (HepG-2) cell line represented a higher sensitivity to peptide 7 (IC50; 3.30 μg/mL), while it appeared insensitive to the rest of the tested peptides. Furthermore, compounds 1, 3, and 5 exhibited a promising safety profile against human normal skin fibroblasts cell line BJ-1. In order to investigate the mode of action, compound 5 was selected as a representative example to study its in vitro effect against the apoptotic parameters and Bax/BCL-2/p53/caspase-7/caspase-3/tubulin, and DNA fragmentation to investigate beta (TUBb). Additionally, all the new analogues were subjected to antimicrobial assay against a panel of Gram-positive and Gram-negative bacteria and the yeast candida Albicans. All the tested GA analogues 1–8 exhibited more antibacterial effect against Micrococcus Luteus than gentamicin, but they exhibited moderate antimicrobial activity against the tested bacterial and yeast strains. Molecular docking studies were also simulated for compound 5 to give better rationalization and put insight to the features of its structure.

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