scholarly journals Epidemiologia e Avaliação de Métodos Diagnósticos em Micoses Superficiais em Serviço de Dermatologia de Hospital Público em Santos, Brasil

2021 ◽  
Vol 79 (4) ◽  
pp. 345-350
Author(s):  
Fernanda J. Bauer ◽  
Letícia Logullo ◽  
Elizabeth M. Heins ◽  
Sandra L. M. Dinato
Keyword(s):  

Introdução: Micoses superficiais são infecções fúngicas causadas principalmente por dermatófitos, leveduras e fungos filamentosos não dermatófitos, que acometem as camadas mais superficiais da pele e seus anexos. Apresentam alta prevalência em todo o mundo.O objectivo deste estudo é avaliar a epidemiologia das micoses superficiais, assim como o índice de concordância entre o exame micológico direto e a cultura para fungos. Métodos: Trata-se de estudo retrospectivo realizado no ambulatório de Dermatologia de hospital terciário, num intervalo de 6 anos. Para a elu- cidação diagnóstica, foi realizada colheita de material através de raspagem ou curetagem, para posterior análise por exame micológico direto e cultura para fungos. Resultados: Foram incluídas 439 amostras de lesões suspeitas de micoses superficiais de 420 pacientes, 268 do sexo feminino (63,8% doentes) com a média de idade de 45,7 anos (dos 3 meses aos 95 anos), com o maior número de casos das unhas (43,4%) e pele glabra (24,1%). Em geral, o fungo mais encontrado na cultura foi o Trichophyton rubrum; no entanto, não houve esta concordância em todos os locais da pele estudados. O exame micológico direto apresentou associação significativamente estatística com a cultura (K=0,955), se eliminadas os casos em que houve contaminação da cultura. Conclusão: O uso do exame micológico direto e da cultura, como métodos diagnósticos na Dermatologia, é uma opção que fornece resultados satisfatórios e de baixo custo, favorecendo doentes e sistema de saúde. Este estudo permitiu descrever o perfil epidemiológico dos pacientes de um centro de Dermatologia de referência, com dados relevantes em relação ao nosso objetivo. A concordância entre o exame micológico direto e a cultura mostrou a confiabilidade dos métodos.

2017 ◽  
Vol 5 (2) ◽  
pp. 79-84
Author(s):  
Anand Pandey ◽  
◽  
Awadhesh Kumar ◽  
Anupam Dikshit ◽  
◽  
...  

Lichens are demarcated as an association of two distinct and dissimilar components; the mycobiont (a fungus) and the phycobionts (an alga) forming a composite organism. The recent advancement in science has paid much attention on the biological roles of lichen secondary substances; which are attributed with a lot of biological activities, such as antibacterial, antifungal, antiviral, anti-inflammatory, anti-tumor, and antioxidant activities. The current research aims to unravel the fungicidal activity of lichen Heterodermia diademata, ethanolic extract (HDE) against prevalent superficial infection causing dermatophytes viz., Trichophyton rubrum, T. mentagrophytes, Microsporum gypseum and M. canis. The HDE was found active against M. gypseum and T. mentagrophytes with MIC; 1.68 and 1.98 mg/ml and mg/ml respectively. The dermatophytes were also analyzed by molecular phylogeny of chs-1 gene for variability in MIC and prediction of susceptible pathogens.


2021 ◽  
Vol 40 (4) ◽  
pp. S482
Author(s):  
E.R. Woolcock ◽  
F. Ara ◽  
S. Bhagra ◽  
C. Lewis ◽  
S. Pettit ◽  
...  

Author(s):  
Yurie Kitauchi ◽  
Yoshiko Kumagai ◽  
Yoko Inoue‐Masuda ◽  
Makoto Sugiura ◽  
Tomotaka Sato ◽  
...  

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 194
Author(s):  
Annabel Guttentag ◽  
Krishothman Krishnakumar ◽  
Nural Cokcetin ◽  
Steven Hainsworth ◽  
Elizabeth Harry ◽  
...  

Superficial dermatophyte infections, commonly known as tineas, are the most prevalent fungal ailment and are increasing in incidence, leading to an interest in alternative treatments. Many floral honeys possess antimicrobial activity due to high sugar, low pH, and the production of hydrogen peroxide (H2O2) from the activity of the bee-derived enzyme glucose oxidase. Australian jarrah (Eucalyptus marginata) honey produces particularly high levels of H2O2 and has been found to be potently antifungal. This study characterized the activity of jarrah honey on fungal dermatophyte species. Jarrah honey inhibited dermatophytes with minimum inhibitory concentrations (MICs) of 1.5–3.5% (w/v), which increased to ≥25% (w/v) when catalase was added. Microscopic analysis found jarrah honey inhibited the germination of Trichophyton rubrum conidia and scanning electron microscopy of mature T. rubrum hyphae after honey treatment revealed bulging and collapsed regions. When treated hyphae were stained using REDOX fluorophores these did not detect any internal oxidative stress, suggesting jarrah honey acts largely on the hyphal surface. Although H2O2 appears critical for the antifungal activity of jarrah honey and its action on fungal cells, these effects persisted when H2O2 was eliminated and could not be replicated using synthetic honey spiked with H2O2, indicating jarrah honey contains agents that augment antifungal activity.


1982 ◽  
Vol 118 (6) ◽  
pp. 446 ◽  
Author(s):  
Robert Michael Cavanaugh
Keyword(s):  

1965 ◽  
Vol 43 (1) ◽  
pp. 30-39 ◽  
Author(s):  
C. T. Bishop ◽  
M. B. Perry ◽  
F. Blank ◽  
F. P. Cooper

A group of polysaccharides, called galactomannans I, were precipitated as their insoluble copper complexes from aqueous solutions of the crude polysaccharides obtained from each of the organisms designated in the title. The five galactomannans I were homogeneous under conditions of electrophoresis and ultracentrifugation and had high positive specific rotations. The major constituent monosaccharide was D-mannose; amounts of D-galactose ranged from nil for the polysaccharide from T. rubrum to 13% for that from T. schönleinii. Methylation and hydrolysis of the five galactomannans I yielded varying amounts of the following: 2,3,5,6-tetra-O-methyl-D-galactose (not present in the products from T. rubrum), 2,3,4,6-tetra-O-methyl-D-mannose, 2,3,4-tri-O-methyl-D-mannose, 2,4,6-tri-O-methyl-D-mannose, 3,4-di-O-methyl-D-mannose, and 3,5-di-O-methyl-D-mannose. Periodate oxidation results agreed with the methylation studies. The gross structural features of each galactomannan I appear to be the same, namely, a basic chain of 1 → 6 linked α-D-mannopyranose units for approximately every 22 of which there is a 1 → 3 linked α-D-mannopyranose residue. Branch points occur along the 1 → 6 linked chain at the C2 positions of the D-mannopyranose units and once in every 45 units at the C2 position of a 1 → 6 linked D-mannofuranose residue. The D-galactose in the polysaccharides is present exclusively as non-reducing terminal furanose units; non-reducing terminal units of D-mannopyranose are also present. The variations in the identities and relative amounts of the non-reducing terminal units were the only apparent differences in the gross structural features within this group of polysaccharides.


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