Fermentation of Oil Palm Trunk Acid Hydrolysate  to Ethanol

Studies on the feasibility of using oil palm trunk acid hyd10lysates as a substrate for ethanol p10duction using the yeast Saccha10myces cerevisiae were carried out in shake flask culture. The effect of pH and fermentation time on the rate of ethanol p10duction were investigated. Results showed that with 3 hours fermentation period, the highest ethanol yield was obtained at pH 4.75. This yield was about 14% of the dry weight of the sample used; thus giving a fermentation efficiency of 94.7%.

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Growth and Oil Production of Apiotrichum curvatum in Tomato Juice‡

Journal of Food Protection ◽

10.4315/0362-028x-61.11.1515 ◽

1998 ◽

Vol 61 (11) ◽

pp. 1515-1517 ◽

Cited By ~ 11

Author(s):

FOLU AKINDUMILA ◽

BONITA A. GLATZ

Keyword(s):

Oil Production ◽

Dry Weight ◽

Tomato Juice ◽

Shake Flask Culture ◽

Flask Culture ◽

Tomato Pulp ◽

Shake Flasks ◽

Casamino Acids ◽

Basal Salts ◽

Apiotrichum Curvatum

The lipid-accumulating yeast Apiotrichum curvatum ATCC 20509 (formerly Candida curvata D) grew in shake flask culture in freshly prepared tomato juice or tomato pulp; growth was improved when the medium was supplemented with sucrose and a basal salts mixture. Under controlled conditions in the fermentor, biomass and oil production reached a maximum at 96 h, compared with 130 h in shake flasks. Biomass production increased with the addition of 0.3% (wt/vol) urea to the juice, but decreased with the addition of 0.5% (wt/vol) yeast extract or Casamino Acids. The total amount of lipid produced and the percent of intracellular oil per cell dry weight were greatest in juice supplemented with 0.3% urea.

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The Production and Optimization of Bioethanol from Oil Palm Fronds: A Source of Renewable Energy

Chemical Science International Journal ◽

10.9734/csji/2021/v30i530232 ◽

2021 ◽

pp. 32-43

Author(s):

I. Eiroboyi ◽

Y. Yerima

Keyword(s):

Aspergillus Niger ◽

Oil Palm ◽

Large Scale ◽

Ethanol Yield ◽

Limiting Factor ◽

Scale Production ◽

Alternative Source ◽

Fermentation Time ◽

Oil Palm Fronds ◽

Palm Fronds

Environmental issues and the desire to be less dependent on fossil fuel have intensified research efforts towards the production of biofuels since they are a safe and clean alternatives to fossil fuels. However, the cost of carbohydrate raw materials has become a limiting factor for large-scale production, hence the need to source for low cost feedstock. This study analyzed the processes and optimization involved in the production of bioethanol from oil palm fronds from Okada, Edo State, Nigeria, as an alternative source of energy. In this study, solid-state fermentation was carried out for the production of fermentable sugars from oil palm fronds inoculated with local isolate Aspergillus niger, the results from this analysis show that the pretreatment of oil palm frond substrate using Aspergillus niger was effective, The process was effectively optimized within the confines of the following parameters; temperature X3 (36-42oC), pH, X2 (5.1-5.7), inoculum, X4(4-22) and fermentation time X1 (0-36hr), an optimum Ethanol yield Y, of 110% was obtained.

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Co-culture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) for bioethanol production using concentrated Prosopis juliflora acid hydrolysate

10.1101/601278 ◽

2019 ◽

Author(s):

Shaik Naseeruddin ◽

Suseelendra Desai ◽

L Venkateswar Rao

Keyword(s):

Saccharomyces Cerevisiae ◽

Ethanol Yield ◽

Culture Method ◽

Pichia Stipitis ◽

Prosopis Juliflora ◽

Bioethanol Production ◽

Dilute Acid Hydrolysis ◽

Total Carbohydrate ◽

Fermentation Efficiency ◽

Acid Hydrolysate

AbstractBioethanol production from lignocellulosic biomass is a viable option for improving energy security and reducing green house emissions. In the present study Prosopis juliflora, an invasive tree found through out India, with total carbohydrate content of 67.4 +/- 6% was selected as lignocellulosic feedstock for bioethanol production. The hydrolysate obtained after biphasic dilute acid hydrolysis contained initial sugar concentration of 18.70 +/- 0.16 g/L and hence to increase the ethanol yield it was concentrated to 33.59 +/- 0.52 g/L (about two-folds) by vacuum distillation. The concentration of sugars, phenols and furans was analyzed before and after concentration process. The concentrated hydrolysate was further detoxified by over liming, neutralization and charcoal treatment and later used for ethanol fermentation by mono- and co culture method. Monoculture of Saccharomyces cerevisiae (VS3) and Pichia stipitis (NCIM 3498) produced 8.52 +/- 0.43 and 4.52 +/- 0.23 g/L of ethanol with 66.21 +/- 3.26% and 60.46 +/- 3.02% of fermentation efficiency, 0.33 +/- 0.02 and 0.31 +/- 0.02 g/g of ethanol yield and 0.24 +/- 0.01 and 0.13 +/- 0.01 g/L/h of productivity, respectively. The co-culture of S. cerevisiae (VS3) and P. stipitis (NCIM 3498) helped to improve all parameters i.e. 10.94 +/- 0.53 g/L of ethanol with fermentation efficiency, ethanol yield and productivity of 83.11 +/- 0.42%, 0.420 +/- 0.01 g/g and 0.30 +/- 0.01 g/L/h, respectively.

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