KUALITAS INDUK UDANG WINDU (Penaeus monodon) DI PERAIRAN PANTAI PANGANDARAN, SELATAN JAWA BARAT

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Suhendar I Sachoemar ◽  
Ratu Siti Aliah

An evaluation of the quality of the Black Tiger Broodstock Shrimp (Penaeus monodon) in the southern coastal area of Pangandaran, West Java was conducted during period of Juny-October 2005. The evaluation was focused on the waterquality identification and the quality of the broodstock collected from the farmer sorround the Pangandaran Area. The result of the evaluation showed that the water quality of the coastal area of Pangandaran was good compare to the northern coastal area of West Java in which the organic and inorganic pollutantwas . This situation was affected on the quality of the  broodstock. The identification result on the quality of broodstock using polymerase chain reaction (PCR) method shows that the broodstock shrimp of the Black Tiger Shrimp(Penaeus monodon) in the coastal area of Pangandaran is free from the White Spot Syndrome Virus (WSSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). From 99 broodstock identified, only 4 indicated by very light contaminated WSSV and 2 by IHHNV. This condition shows that in general the quality of the broodstock shrimp of the Black Tiger Shrimp (Penaeus monodon) in the coastal area of Pangandaran was excellent and these sources are potentialto be applied as broodstock sources for developing the Black Tiger Shrimp (Penaeus monodon) culture in the national level.

2015 ◽  
Vol 12 (2) ◽  
pp. 101
Author(s):  
Irma Suriyani ◽  
Ince Ayu Khairana Kadriah ◽  
Ilmiah Kuruseng

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph"> </p><p class="BasicParagraph">This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic <em>Vibrio</em> on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with <em>Vibrio harveyi</em>. The PL of tiger shrimp were infected with 10<sup>6</sup> cfu/mL of <em>V. harveyi</em> by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA <em>Vibrio</em> were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of <em>Vibrio</em> was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on <em>Vibrio</em> pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of <em>V. harveyi</em>.</p><p class="BasicParagraph"> </p><p class="BasicParagraph">Keywords: specific primer, luminous <em>Vibrio</em> bacteria, pathogenic, PCR method, hemolysin gene</p><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"> </p><p class="BasicParagraph">Penelitian ini bertujuan untuk mengetahui kemampuan atau sensitivitas primer hemolisin dalam mendeteksi <em>Vibrio</em> patogen dengan lama pemaparan berbeda. Penelitian ini dilakukan dengan menginfeksikan <em>Vibrio harveyi</em> pada benur udang dengan metode perendaman pada konsentrasi 10<sup>6</sup> cfu/mL. Pengambilan sampel dilakukan pada waktu tiga, enam, 12, 24, 48, dan 72 jam pascainfeksi. Keberadaan gen hemolisin pada bakteri <em>V. harveyi</em> dideteksi menggunakan teknik <em>polymerase chain reaction </em>(PCR). Hasil elektroforesis memperlihatkan bahwa pada pemaparan tiga dan enam jam keberadaan gen hemolisin dari bakteri <em>Vibrio</em> patogen yang diinfeksikan sudah dapat terdeteksi pada benur walaupun masih terlihat tipis. Pada pemaparan 12 jam terlihat sangat jelas pita-pita DNA dari bakteri patogen. Sedangkan pada pemaparan 24, 48, dan 72 jam sudah tidak terdeteksi lagi gen hemolisin dari bakteri <em>Vibrio</em>. Hal ini diduga disebabkan terjadinya penurunan populasi bakteri <em>Vibrio</em> yang hidup dalam tubuh dan media pemeliharaan udang. Pentingnya deteksi cepat diawal udang terinfeksi bakteri (3–12 jam) karena setelah 12 jam infeksi sudah sulit untuk mendeteksi keberadaan bakteri patogen di dalam tubuh udang.</p><p class="BasicParagraph"> </p><p>Kata kunci: primer spesifik, bakteri <em>Vibrio</em> berpendar, patogenik, metode PCR, gen hemolisin</p><p> </p>


2020 ◽  
Vol 51 (6) ◽  
pp. 2197-2204
Author(s):  
Song Jiang ◽  
Dongliang Liu ◽  
Falin Zhou ◽  
Xianbin Mo ◽  
Qibin Yang ◽  
...  

Author(s):  
Le Nhat Tam ◽  
Nguyen Nam Giao ◽  
Tran Thi Ngoc Nhung ◽  
Đao Bich Duyen ◽  
Le Thi Hong Nhung ◽  
...  

<p>Black tiger shrimps (<em>Penaeus monodon</em>) wereharvested andstored at 0<sup>0</sup>Cfor study. The quality index method (QIM) was developed to evaluate the freshness of black tiger shrimp, and was presented in this study. The terms used to describe all the changes related to the texture, color, and odor are carefully chosen. The development of a QIM scheme included three main steps:A preliminary scheme to evaluate all the changes of attributes related to quality – Final QIM scheme and training – Validation of QIM scheme and comparison estimate the remaining shelf life and actual shelf life. The results show that shelf life of black tiger shrimp in the ice storage is 8 days. Quality score involving attributes decreases during the storage timeand have a correlation close to the linear regression equation. The scheme validation quality of black tiger shrimp by QIM method allows the estimation of the remaining shelf life.</p>


Aquaculture ◽  
2019 ◽  
Vol 499 ◽  
pp. 160-166 ◽  
Author(s):  
M.J. Sellars ◽  
J.A. Cowley ◽  
D. Musson ◽  
M. Rao ◽  
M.L. Menzies ◽  
...  

2017 ◽  
Vol 126 (1B) ◽  
pp. 19 ◽  
Author(s):  
Le Nhat Tam ◽  
Nguyen Nam Giao ◽  
Tran Thi Ngoc Nhung ◽  
Đao Bich Duyen ◽  
Le Thi Hong Nhung ◽  
...  

<p>Black tiger shrimps (<em>Penaeus monodon</em>) wereharvested andstored at 0<sup>0</sup>Cfor study. The quality index method (QIM) was developed to evaluate the freshness of black tiger shrimp, and was presented in this study. The terms used to describe all the changes related to the texture, color, and odor are carefully chosen. The development of a QIM scheme included three main steps:A preliminary scheme to evaluate all the changes of attributes related to quality – Final QIM scheme and training – Validation of QIM scheme and comparison estimate the remaining shelf life and actual shelf life. The results show that shelf life of black tiger shrimp in the ice storage is 8 days. Quality score involving attributes decreases during the storage timeand have a correlation close to the linear regression equation. The scheme validation quality of black tiger shrimp by QIM method allows the estimation of the remaining shelf life.</p>


2018 ◽  
Vol 56 (6) ◽  
pp. 773-777
Author(s):  
Le Nhat Tam ◽  
Phan Thuy Xuan Uyen ◽  
Truong Huynh Anh Vu

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