scholarly journals Comparative Mass Spectrometric Study of Biologically Active Substances in the Roots of Far Eastern Panax Ginseng C.A. Meyer Obtained by Supercritical Extraction

Food Industry ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 65-72
Author(s):  
Maya Razgonova ◽  
Tatiana Kalenik ◽  
Aleksander Zakharenko ◽  
Giancarlo Cravotto ◽  
Kirill Golokhvas
Keyword(s):  
Panax Ginseng ◽  
Mass Spectrometric Study ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Supercritical Extraction ◽  
Spectrometric Study ◽  
Far Eastern ◽  
Active Substances

scholarly journals Brown algae of the orders Laminariales and Fucales from the Sakhalin-Kuril region: stocks, extraction, use

Trudy VNIRO ◽  
2020 ◽  
Vol 181 ◽  
pp. 235-256
Author(s):  
A.V. Podkorytova ◽  
◽  
A.N. Roshchina ◽  
N.V. Evseeva ◽  
A.I. Usov ◽  
...  
Keyword(s):  
Brown Algae ◽  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Components ◽  
Industrial Processing ◽  
Forced Air ◽  
Far Eastern ◽  
Air Circulation ◽  
Active Substances

Data on distribution, stocks of commercial and potentially commercial brown algae in the Sakhalin-Kuril region, their extraction, industrial processing, and chemical composition are presented. It is shown that for the preservation of biologically active substances and technically valuable components of laminaria, the use of an IR dryer for its preservation at LLC FIG «BINOM», Aniva, Island Sakhalin was an innovative technological solution. The products that are produced at this enterprise, in particular, dried shredded laminaria (IR drying), dried thalloms in heat dryers equipped with gas air heaters with forced air circulation are high — quality products containing all the biologically active components of natural brown alga. Expanded quality indicators of dried products from the laminaria of Sakhalin-Kuril region are presented. Based on the obtained data, recommendations were developed for the integrated use of algae as an annually renewable aquatic biological resource, traditionally extracted and used as raw materials in the production of food products and biologically active substances. It is shown that, despite the significant reserves of kelp in the far Eastern seas of Russia, they are currently poorly developed by fishing, with the exception of the coastal zone of Western Sakhalin.

scholarly journals Panax ginseng callus, suspension, and root cultures: extraction and qualitative analysis

2020 ◽  
Vol 8 (2) ◽  
pp. 369-376
Author(s):  
Lyubov Dyshlyuk ◽  
Anastasia Dmitrieva ◽  
Svetlana Ivanova ◽  
Yuliya Golubtsova ◽  
Lev Ostroumov
Keyword(s):  
Qualitative Analysis ◽  
Organic Compounds ◽  
Panax Ginseng ◽  
Callus Cultures ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Root Cultures ◽  
Optimal Parameters ◽  
Promising Source ◽  
Active Substances

Introduction. In recent years, scientists have been actively searching for medicinal plants containing biologically active substances with geroprotective properties to treat diseases of old age, in particular cancer, diabetes, cardiovascular diseases, and others. Ginseng (Panax ginseng L.) is a promising source of geroprotective compounds. We aimed to select optimal parameters for extracting organic compounds from ginseng callus, suspension, and root cultures and analyze their qualitative composition. Study objects and methods. We studied ginseng callus, suspension, and root cultures, as well as their extracts. Biologically active substances were extracted with 30 to 70% ethanol. Organic compounds were determined by thin-layer chromatography. The results for each plant were archived and analyzed for the presence of quercetin, mangiferin, luteolin, rutin, quercetin-2-D-glucoside, malvidin, as well as caffeic, cinnamic, ferulic, and sinapinic acids. Results and discussion. We developed a procedure for screening solvents and performed a fractional qualitative analysis of biologically active substances extracted from ginseng. As a result, we established the optimal parameters for extracting biologically active substances from the dried biomass of ginseng cultures. In all cases, temperature and the ratio of solvent to biomass were the same (50°C, 1:5). However, the extraction time and ethanol concentration differed, amounting to 60 min and 50% for callus cultures, 30 min and 60% for suspension cultures, and 60 min and 70% for root cultures. The qualitative analysis of organic compounds showed the presence of rutin (0.25), quercetin (0.75), and mangiferin (0.57), as well as caffeic and sinapinic acids in the extracts. Conclusion. Our set of experiments to isolate biologically active substances from ginseng callus, suspension, and root cultures resulted in selecting the optimal extraction parameters and analyzing the extracts for the presence of organic compounds.

scholarly journals ZOOHYGIENIC CHARACTERISTICS OF BIOTECHNOLOGICAL METHODS OF METABOLISM REGULATING OF CHICKENS EMBRYO IN THE PROCESS OF INCUBATION

2020 ◽  
pp. 100-105
Author(s):  
Olga Bordunova ◽  
Evgeniya Samokhina ◽  
Leontiі Khmelnychyi ◽  
Mykola Povod ◽  
Victoria Vechorka
Keyword(s):  
Mass Spectrometer ◽  
Gas Permeability ◽  
Experimental Studies ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Hatching Eggs ◽  
Active Substances

The research presents the results of experimental studies of the influence of various physicochemical factors (phonophoresis, electrophoresis, electrospray, diffusion, etc.) on the rate of transport of biologically active substances through the protective layer of hatchig eggs shell of crossbred Haysex Brown. The aim of the study was to compare the effectiveness of the use of physicochemical methods of transportation of biologically active substances through the shell of hatching eggs of chickens. For the experiment, three batches of eggs were formed, which were obtained from layer hens Haysex Brown, 144 pieces in each experimental group. The kinetic parameters of BAS transport through bioceramic protective barriers of eggs were calculated based on the determination of BAS concentration on the surface and inside the egg by mass spectrometric method (mass spectrometer with ionization of 252Cf fission fragments "MSBH", (Ltd "SELMI", Sumy, Ukraine). The degree of permeability of bioceramic layers of the shell relative to the model gas mixture which is identical to the atmospheric air was studied by the method of V. Breslavets et other and the mass spectrometric method (gas mass spectrometer "MX 7304A"). Electron microscopic studies were performed on a scanning electron microscope REMMA-102; Visilog 6.11 (Noesis, Belgium) was used to process the obtained digital images to determine the number of shell microdefects per unit area of the digital image. It has been experimentally proved that to increase the efficiency of transport of biologically active substances (BAS) through the bioceramic layers of the shell of hatching eggs of Loman Brown hens, it is advisable to use ultrasonic treatment (phonophoresis, sonophoresis), substances - enhancers, including plant terpenes (L-menthol), DMSO and cyclodextrin. Phonophoretic treatment of hatching eggs increases the hatchability of eggs by 7.6%. At the same time, the gas permeability of the bioceramic layer increases significantly (by 0.43. 10-4 m3 / m2 s).

SUPERCRITICAL EXTRACTION FOR THE ISOLATION OF BIOLOGICALLY ACTIVE SUBSTANCES

2021 ◽  
Vol 1 (19) ◽  
pp. 185-187
Author(s):  
I.V. Kazeev ◽  
I.I. Khudeev ◽  
O.A. Bocharova
Keyword(s):  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Supercritical Extraction ◽  
Plant Raw Materials ◽  
Materials Research ◽  
Active Substances

The paper presents the developing direction of supercritical extraction (SCE), which opens up new prospects for the extraction of biologically active substances from plant raw materials. Research is underway to obtain active biological substances for the treatment of socially significant diseases.

scholarly journals Study on the composition of fatty and organic acids of the feverfew herb (Tanacetum Parthenium (L.) Schultz Bip.)

2020 ◽  
pp. 61-67
Author(s):  
К. Р. Гордєй ◽  
Т. М. Гонтова
Keyword(s):  
Organic Acids ◽  
Carboxylic Acids ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Mass Spectrometric Method ◽  
Qualitative Composition ◽  
Tanacetum Parthenium ◽  
Quantitative Content ◽  
Active Substances

In recent years, herbal medicines have become increasingly popular for the treatment of many diseases because they have a complex and multifactorial influence on the body. Fatty and organic acids are important groups of biologically active substances for the improvement of many pathological human’s conditions. Feverfew (Tanacetum parthenium (L.) Schultz Bip.) is a promising source of biologically active substances with many years of success in medicine and insufficiently studied chemical composition. The analysis of fatty and organic acids is one of the directions of our comprehensive study of the feverfew herb grown in Ukraine. The aim of the work was to conduct a study of the qualitative composition and quantitative content of fatty and organic acids in the feverfew herb grown in Ukraine. For the study, we used the feverfew herb, which was grown in the scientific fields of the Botanical Garden of the National University of Ukraine (2019). The studies were carried out by a gas chromatography-mass spectrometric method on an Agilent Technologies 6890N chromatograph with a 5973 mass spectrometric detector. 35 carboxylic acids were found and identified in the samples. Among them 21 acids are saturated, 5 are unsaturated and 9 are aromatic. The total content of carboxylic acids in feverfew herb was 26.54 mg/g. Among the identified fatty and organic acids in the feverfew herb the greatest amount of the following acids was observed: succinic (4.89 mg/g), levulinic (4.34 mg/g), citric (2.73 mg/g), palmitic (2.15 mg/g), linoleic (1.71 mg/g), oxalic (1.53 mg/g) and linolenic (1.07 mg/g) acids. Among the aromatic acids in the feverfew herb the dominant compounds were p-coumaric (2.4%), benzoic (1.89%) and ferulic (1.40%). For the first time, a chromatography-mass spectrometric determination of the qualitative composition and quantitative content of organic and fatty acids in feverfew herb collected in Ukraine was carried out. The data obtained indicate a varied composition and rich content of acids in domestic raw materials and will be used to create medicines based on the feverfew herb.

scholarly journals ZOOHYGIENIC CHARACTERISTICS OF BIOTECHNOLOGICAL METHODS OF METABOLISM REGULATING OF CHICKENS EMBRYO IN THE PROCESS OF INCUBATION

2021 ◽  
pp. 36-41
Author(s):  
Olga Bordunova ◽  
Evgeniya Samokhina ◽  
Leontiy Khmelnychyi ◽  
Mykola Povod ◽  
Victoria Vechorka
Keyword(s):  
Mass Spectrometer ◽  
Gas Permeability ◽  
Experimental Studies ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Hatching Eggs ◽  
Active Substances

  The research presents the results of experimental studies of the influence of various physicochemical factors (phonophoresis, electrophoresis, electrospray, diffusion, etc.) on the rate of transport of biologically active substances through the protective layer of hatching eggs shell of crossbred Haysex Brown. The aim of the study was to compare the effectiveness of the use of physicochemical methods of transportation of biologically active substances through the shell of hatching eggs of chickens. For the experiment, three batches of eggs were formed, which were obtained from layer hens Haysex Brown, 144 pieces in each experimental group. The kinetic parameters of BAS transport through bioceramic protective barriers of eggs were calculated based on the determination of BAS concentration on the surface and inside the egg by mass spectrometric method (mass spectrometer with ionization of 252Cf fission fragments "MSBH", (Ltd "SELMI", Sumy, Ukraine). The degree of permeability of bioceramic layers of the shell relative to the model gas mixture which is identical to the atmospheric air was studied by the method of V. Breslavets et other and the mass spectrometric method (gas mass spectrometer "MX 7304A"). Electron microscopic studies were performed on a scanning electron microscope REMMA-102; Visilog 6.11 (Noesis, Belgium) was used to process the obtained digital images to determine the number of shell microdefects per unit area of the digital image. It has been experimentally proved that to increase the efficiency of transport of biologically active substances (BAS) through the bioceramic layers of the shell of hatching eggs of Loman Brown hens, it is advisable to use ultrasonic treatment (phonophoresis, sonophoresis), substances - enhancers, including plant terpenes (L-menthol), DMSO and cyclodextrin. Phonophoretic treatment of hatching eggs increases the hatchability of eggs by 7.6%. At the same time, the gas permeability of the bioceramic layer increases significantly (by 0.43. 10-4 m3 / m2 s).

Microscopic fungi of lithobiont communities of Argentine Islands Region: Data from the 22nd Ukrainian Antarctic Expedition

2020 ◽  
Vol 62 (1-2) ◽  
pp. 49-68
Author(s):  
T. O. Kondratiuk ◽  
T. V. Beregova ◽  
I. Yu. Parnikoza ◽  
S. Y. Kondratyuk ◽  
A. Thell
Keyword(s):  
Filamentous Fungi ◽  
Mucor Circinelloides ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Microscopic Fungi ◽  
Rhodotorula Rubra ◽  
Yeast Fungi ◽  
Antarctic Expedition ◽  
Geomyces Pannorum ◽  
Active Substances

The identification of the diversity of microscopic fungi of lithobiont communities of the Argentine Islands in specimens collected during the 22nd Ukrainian Antarctic Expedition was the purpose of this work. Samples of rock, soil, mosses and lichens of rock micro-habitats of “Crustose lichen sub-formation and fruticose lichen and moss cushion sub-formation” were used in the work. These samples were used for extracting and cultivation of filamentous fungi on dense nutrient media. Determination of physiological and biochemical characteristics and identification of yeast-like fungi were performed using a microbiological analyser ‘Vitek-2’ (‘Bio Merieux’, France). Cultivation of microorganisms was carried out at temperatures from +2 to +37 °C. In results cultures of microscopic fungi of Zygomycota (Mucor circinelloides), Ascomycota (species of the genera cf. Tlielebolus, Talaromyces), representatives of the Anamorphic fungi group (Geomyces pannorum, species of the genera Alternaria, Acremonium, Aspergillus, Penicillium, and Cladosporium) were isolated from Antarctic samples. Microscopic fungi Penicillium spp. were dominated after the frequency in the studied samples (54.5%). Rhodotorula rubra and Candida sp. among isolated yeast fungi, and dark pigmented fungi represented by Aureobasidium pulhdans and Exophiala spp. were identified. The biological properties of a number of isolated fungi (the potential ability to synthesise important biologically active substances: melanins, carotenoids, lipids) are characterised. Mycobiota of rock communities of Argentine Islands is rich on filamentous and yeast fungi similarly to other regions of Antarctica. A number of fungi investigated are potentially able to synthesise biologically active substances. The dark pigmented species of the genera Cladosporium, Exophiala, Aureobasidium pulhdans, capable of melanin synthesis; ‘red’ yeast Rhodotorula rubra (carotenoid producers and resistant to toxic metals); Mucor circinelloides and Geomyces pannorum, lipid producers, are among these fungi. Yeast-like fungi assimilated a wide range of carbohydrates, which will allow them to be further used for cultivation in laboratory and process conditions. The collection of technologically promising strains of microorganisms, part of the Culture Collection of Fungi at Taras Shevchenko National University of Kyiv (Ukraine), is updated with isolated species (strains) of filamentous fungi and yeast – potential producers of biologically active substances, obtained within this study.

Sign in / Sign up

Export Citation Format

Share Document