scholarly journals Study on the composition of fatty and organic acids of the feverfew herb (Tanacetum Parthenium (L.) Schultz Bip.)

2020 ◽  
pp. 61-67
Author(s):  
К. Р. Гордєй ◽  
Т. М. Гонтова
Keyword(s):  
Organic Acids ◽  
Carboxylic Acids ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Mass Spectrometric Method ◽  
Qualitative Composition ◽  
Tanacetum Parthenium ◽  
Quantitative Content ◽  
Active Substances

In recent years, herbal medicines have become increasingly popular for the treatment of many diseases because they have a complex and multifactorial influence on the body. Fatty and organic acids are important groups of biologically active substances for the improvement of many pathological human’s conditions. Feverfew (Tanacetum parthenium (L.) Schultz Bip.) is a promising source of biologically active substances with many years of success in medicine and insufficiently studied chemical composition. The analysis of fatty and organic acids is one of the directions of our comprehensive study of the feverfew herb grown in Ukraine. The aim of the work was to conduct a study of the qualitative composition and quantitative content of fatty and organic acids in the feverfew herb grown in Ukraine. For the study, we used the feverfew herb, which was grown in the scientific fields of the Botanical Garden of the National University of Ukraine (2019). The studies were carried out by a gas chromatography-mass spectrometric method on an Agilent Technologies 6890N chromatograph with a 5973 mass spectrometric detector. 35 carboxylic acids were found and identified in the samples. Among them 21 acids are saturated, 5 are unsaturated and 9 are aromatic. The total content of carboxylic acids in feverfew herb was 26.54 mg/g. Among the identified fatty and organic acids in the feverfew herb the greatest amount of the following acids was observed: succinic (4.89 mg/g), levulinic (4.34 mg/g), citric (2.73 mg/g), palmitic (2.15 mg/g), linoleic (1.71 mg/g), oxalic (1.53 mg/g) and linolenic (1.07 mg/g) acids. Among the aromatic acids in the feverfew herb the dominant compounds were p-coumaric (2.4%), benzoic (1.89%) and ferulic (1.40%). For the first time, a chromatography-mass spectrometric determination of the qualitative composition and quantitative content of organic and fatty acids in feverfew herb collected in Ukraine was carried out. The data obtained indicate a varied composition and rich content of acids in domestic raw materials and will be used to create medicines based on the feverfew herb.

scholarly journals ZOOHYGIENIC CHARACTERISTICS OF BIOTECHNOLOGICAL METHODS OF METABOLISM REGULATING OF CHICKENS EMBRYO IN THE PROCESS OF INCUBATION

2020 ◽  
pp. 100-105
Author(s):  
Olga Bordunova ◽  
Evgeniya Samokhina ◽  
Leontiі Khmelnychyi ◽  
Mykola Povod ◽  
Victoria Vechorka
Keyword(s):  
Mass Spectrometer ◽  
Gas Permeability ◽  
Experimental Studies ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Hatching Eggs ◽  
Active Substances

The research presents the results of experimental studies of the influence of various physicochemical factors (phonophoresis, electrophoresis, electrospray, diffusion, etc.) on the rate of transport of biologically active substances through the protective layer of hatchig eggs shell of crossbred Haysex Brown. The aim of the study was to compare the effectiveness of the use of physicochemical methods of transportation of biologically active substances through the shell of hatching eggs of chickens. For the experiment, three batches of eggs were formed, which were obtained from layer hens Haysex Brown, 144 pieces in each experimental group. The kinetic parameters of BAS transport through bioceramic protective barriers of eggs were calculated based on the determination of BAS concentration on the surface and inside the egg by mass spectrometric method (mass spectrometer with ionization of 252Cf fission fragments "MSBH", (Ltd "SELMI", Sumy, Ukraine). The degree of permeability of bioceramic layers of the shell relative to the model gas mixture which is identical to the atmospheric air was studied by the method of V. Breslavets et other and the mass spectrometric method (gas mass spectrometer "MX 7304A"). Electron microscopic studies were performed on a scanning electron microscope REMMA-102; Visilog 6.11 (Noesis, Belgium) was used to process the obtained digital images to determine the number of shell microdefects per unit area of the digital image. It has been experimentally proved that to increase the efficiency of transport of biologically active substances (BAS) through the bioceramic layers of the shell of hatching eggs of Loman Brown hens, it is advisable to use ultrasonic treatment (phonophoresis, sonophoresis), substances - enhancers, including plant terpenes (L-menthol), DMSO and cyclodextrin. Phonophoretic treatment of hatching eggs increases the hatchability of eggs by 7.6%. At the same time, the gas permeability of the bioceramic layer increases significantly (by 0.43. 10-4 m3 / m2 s).

scholarly journals ZOOHYGIENIC CHARACTERISTICS OF BIOTECHNOLOGICAL METHODS OF METABOLISM REGULATING OF CHICKENS EMBRYO IN THE PROCESS OF INCUBATION

2021 ◽  
pp. 36-41
Author(s):  
Olga Bordunova ◽  
Evgeniya Samokhina ◽  
Leontiy Khmelnychyi ◽  
Mykola Povod ◽  
Victoria Vechorka
Keyword(s):  
Mass Spectrometer ◽  
Gas Permeability ◽  
Experimental Studies ◽  
Mass Spectrometric ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Hatching Eggs ◽  
Active Substances

  The research presents the results of experimental studies of the influence of various physicochemical factors (phonophoresis, electrophoresis, electrospray, diffusion, etc.) on the rate of transport of biologically active substances through the protective layer of hatching eggs shell of crossbred Haysex Brown. The aim of the study was to compare the effectiveness of the use of physicochemical methods of transportation of biologically active substances through the shell of hatching eggs of chickens. For the experiment, three batches of eggs were formed, which were obtained from layer hens Haysex Brown, 144 pieces in each experimental group. The kinetic parameters of BAS transport through bioceramic protective barriers of eggs were calculated based on the determination of BAS concentration on the surface and inside the egg by mass spectrometric method (mass spectrometer with ionization of 252Cf fission fragments "MSBH", (Ltd "SELMI", Sumy, Ukraine). The degree of permeability of bioceramic layers of the shell relative to the model gas mixture which is identical to the atmospheric air was studied by the method of V. Breslavets et other and the mass spectrometric method (gas mass spectrometer "MX 7304A"). Electron microscopic studies were performed on a scanning electron microscope REMMA-102; Visilog 6.11 (Noesis, Belgium) was used to process the obtained digital images to determine the number of shell microdefects per unit area of the digital image. It has been experimentally proved that to increase the efficiency of transport of biologically active substances (BAS) through the bioceramic layers of the shell of hatching eggs of Loman Brown hens, it is advisable to use ultrasonic treatment (phonophoresis, sonophoresis), substances - enhancers, including plant terpenes (L-menthol), DMSO and cyclodextrin. Phonophoretic treatment of hatching eggs increases the hatchability of eggs by 7.6%. At the same time, the gas permeability of the bioceramic layer increases significantly (by 0.43. 10-4 m3 / m2 s).

scholarly journals Determination of the Qualitative Composition of Biologically Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhaponticum carthamoides

2021 ◽  
Vol 11 (6) ◽  
pp. 2555
Author(s):  
Lyudmila Asyakina ◽  
Svetlana Ivanova ◽  
Alexander Prosekov ◽  
Lyubov Dyshlyuk ◽  
Evgeny Chupakhin ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cell Suspension ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Root Cultures ◽  
Qualitative Composition ◽  
Callus Cell ◽  
Rhaponticum Carthamoides ◽  
Active Substances

This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitative and quantitative analysis of BAS. The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension and root cultures showed that the main biologically active substances in the medicinal plant Rhaponticum carthamoides are 2-deoxy-5,20,26-trihydroxyecdyson (7 mg, yield 0.12%), 5,20,26-trihydroxyecdyson 20,22-acetonide (15 mg, yield 0.25%), 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide (6 mg, yield 0.10%), 20,26-dihydroxyecdyson 20,22-acetonidecdyson 20,22-acetonide (5 mg, yield 0.09%), and ecdyson 20,22-acetonide (6 mg, yield 0.10%). In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides, for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects.

scholarly journals INFLUENCE OF CONSERVATION METHOD (FREEZING AND DRYING) ON COMPOSITION AND CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN ROSACEAE FRUITS (CRUDE HERBAL DRUGS)

2019 ◽  
Vol 12 (1) ◽  
pp. 542
Author(s):  
Ekaterina Vyacheslavovna Sergunova ◽  
Dmitry Olegovich Bokov
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Oven Drying ◽  
Total Anthocyanins ◽  
Active Substances ◽  
Conservation Method

Objective: Now, five crude herbal drugs (CHD) of fruit morphological group (fruits) related to Rosaceae family included in the Russian State Pharmacopoeia XI and XIII editions: Fructus Rosae (Rosa cinnamomea L. and other species), Fructus Crataegi (Crataegus sanguinea Pall. and other species), Fructus Sorbi (Sorbus aucuparia L.), Fructus Rubi (Rubus idaeus L.), and Fructus Aroniae (Aronia melanocarpa [Michx.] Elliott).The aim of this study was to investigate the effect of freezing and oven drying on the composition and content of individual groups of biologically active substances (BAS) in Rosaceae fruits.Methods: The groups of BAS were investigated by UV spectrophotometry (flavonoids and anthocyanins), galvanostatic coulometry titration (organic acids), gravimetry (polysaccharides), and titration with potassium permanganate (tannins).Results: The relation between content of BAS (total free organic acids, total flavonoids, total anthocyanins, total tannins, and total polysaccharides) and fruits conservation method was determined. In frozen fruits total polysaccharides, total free organic acids, ascorbic acid content decreases by 5–7%, total flavonoids and total tannins content - 10–15%, total anthocyanins content - 25–30%. Fruits oven drying at 60–80°C leads to more significant losses of total anthocyanins, ascorbic acid, and total free organic acids.Conclusion: The study of BAS stability in frozen and dried fruits was lasting for 12 months. The duration of shelf life for frozen fruits is recommended. Freezing as a conservation method allows to preserve more BAS in Rosaceae fruits. Frozen CHD can be proposed as an alternative to fresh and dried СРВ for the preparation of allopathic and homeopathic medicines.

scholarly journals INFLUENCE OF CONSERVATION METHOD (FREEZING AND DRYING) ON COMPOSITION AND CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN ROSACEAE FRUITS (CRUDE HERBAL DRUGS)

2019 ◽  
Vol 12 (1) ◽  
pp. 542
Author(s):  
Ekaterina Vyacheslavovna Sergunova ◽  
Dmitry Olegovich Bokov
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Herbal Drugs ◽  
Oven Drying ◽  
Total Anthocyanins ◽  
Active Substances ◽  
Conservation Method

Objective: Now, five crude herbal drugs (CHD) of fruit morphological group (fruits) related to Rosaceae family included in the Russian State Pharmacopoeia XI and XIII editions: Fructus Rosae (Rosa cinnamomea L. and other species), Fructus Crataegi (Crataegus sanguinea Pall. and other species), Fructus Sorbi (Sorbus aucuparia L.), Fructus Rubi (Rubus idaeus L.), and Fructus Aroniae (Aronia melanocarpa [Michx.] Elliott).The aim of this study was to investigate the effect of freezing and oven drying on the composition and content of individual groups of biologically active substances (BAS) in Rosaceae fruits.Methods: The groups of BAS were investigated by UV spectrophotometry (flavonoids and anthocyanins), galvanostatic coulometry titration (organic acids), gravimetry (polysaccharides), and titration with potassium permanganate (tannins).Results: The relation between content of BAS (total free organic acids, total flavonoids, total anthocyanins, total tannins, and total polysaccharides) and fruits conservation method was determined. In frozen fruits total polysaccharides, total free organic acids, ascorbic acid content decreases by 5–7%, total flavonoids and total tannins content - 10–15%, total anthocyanins content - 25–30%. Fruits oven drying at 60–80°C leads to more significant losses of total anthocyanins, ascorbic acid, and total free organic acids.Conclusion: The study of BAS stability in frozen and dried fruits was lasting for 12 months. The duration of shelf life for frozen fruits is recommended. Freezing as a conservation method allows to preserve more BAS in Rosaceae fruits. Frozen CHD can be proposed as an alternative to fresh and dried СРВ for the preparation of allopathic and homeopathic medicines.

QUALITATIVE AND QUANTITATIVE ANALYSIS OF FLAVONOIDS DURING DEVELOPMENT OF ANTIDIABETIC EXTRACT

2021 ◽  
pp. 45-50
Author(s):  
D. Aleksandrova ◽  
A. Legosteva
Keyword(s):  
Biologically Active Substances ◽  
Biologically Active ◽  
Qualitative And Quantitative Analysis ◽  
Flavonoid Content ◽  
Qualitative And Quantitative ◽  
Quantitative Content ◽  
Vaccinium Myrtillus L ◽  
Active Substances ◽  
Water Alcohol ◽  
Alcohol Extraction

During development of antidiabetic extract in blueberry shoots (Vaccinium myrtillus L.), ginseng leaves (Panax ginseng C.A.Mey.) and water-alcohol extraction from this plant material the flavonoid content is studied because this group of biologically active substances has hypoglycemic effect. Qualitative reactions for different groups of flavonoids has confirmed their presence in blueberry shoots and ginseng leaves. In addition, qualitative analysis of water-alcohol extraction on their base using spectrophotometry has also showed presence of this group of biologically active substances. Along with that, quantitative content of flavonoids has been detected using differential spectrophotometry in blueberry shoots, ginseng leaves and water-alcohol extraction on their base.

scholarly journals Determination of the Qualitative Composition of Biologically-Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhodiola rosea

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 365 ◽  
Author(s):  
Lyudmila Asyakina ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Alexander Prosekov ◽  
Elena Ulrikh ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cell Suspension ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Root Cultures ◽  
Qualitative Composition ◽  
Callus Cell ◽  
Active Substances

The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension, and root cultures show that the main biologically active substances (BAS) in the medicinal plant, Rhodiola rosea, are 6-C-(1-(4-hydroxyphenyl)ethyl)aromadendrin (25 mg, yield 0.21%), 2-(3,7-dihydroxy-2-(2-hydroxypropan-2-yl)-2,3-dihydrobenzofuran-5-yl)-6,7-dihydroxychroman-4-one (23 mg, yield 0.2%), 2-(3,4-dimethoxyphenyl)-5,7-dimethoxychroman-4-one (175 mg, yield 1.5%), 5,7-dihydroxy-2-(4-hydroxy-3-(2-(4-hydroxyphenyl)-4-oxo-4H-chromen-6-yl)phenyl)-4H-chromen-4-one (45 mg, yield 0.5%), 5,6,7,8-tetrahydroxy-4-methoxyflavone (0.35 mg, 0.5%). BAS from the dried biomass extracts of in vitro callus, cell suspension, and root cultures of Rhodiola rosea will be used for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial, and antioxidant effects.

scholarly journals Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract

2021 ◽  
Vol 23 (1) ◽  
pp. 132-137
Author(s):  
O. Yu. Maslov ◽  
S. V. Kolisnyk ◽  
O. V. Hrechana ◽  
A. H. Serbin
Keyword(s):  
Dietary Supplements ◽  
Green Tea ◽  
Leaf Extract ◽  
Green Tea Extract ◽  
Biologically Active ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
Tea Extract ◽  
Tea Leaf ◽  
Active Substances

Recently, there has been a high demand from the population for dietary supplements that have antioxidant activity. First of all, these are dietary supplements with green tea leaf extract, because green tea contains catechins in a fairly high amount. Nowadays, according to the current legislation of Ukraine the qualitative composition and quantitative content of biologically active substances in dietary supplements are not established, which to a certain extent affects the quality and safety of these products. The aim of the study was to determine the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Materials and methods. Three dietary supplements from different manufacturers “Green Tea Extract”, “Green Tea Extract”, “Green Tea” were chosen for the study. Qualitative analysis was performed by thin layer chromatography, for quantification spectrophotometry in the UV-region was used. Results. The presence of catechins was found in the studied dietary supplements, caffeine was found only in trace amounts. The total content of catechins is 146.80 ± 1.36 mg, 79.00 ± 0.88 mg, 28.00 ± 0.75 mg, and in terms of green tea extract, which is stated in dietary supplements for dietary supplements “Green Tea Extract”, “Green Tea Extract”, “Green Tea”, respectively. Conclusions. Qualitative and quantitative analysis of some groups of BAS of three dietary supplements with green tea leaf extract was performed. It was found that dietary supplements “Green Tea Extract” and “Green Tea” meet the requirements of the US Pharmacopoeia 38 in terms of catechins. Based on the study, it can be concluded that the problem of compliance with dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

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