EFFECT OF ETIOLATION AND MEDIUM COMPOSITION ON RHYZOGENESIS OF APPLE ROOTSTOCK MICROPLANTS IN IN VITRO CULTURE

It was established that for in vitro rhyzogenesis of apple rootstock 106-13 it is better to use medium with 1/2 MS macro- and microsalts supplemented with IBA in concentration of 0.5 mg/l without use of etiolation (the yield of rooted microplants was 90.0 % with a coefficient of root system development – 1.14 ± 0.18). For in vitro rhyzogenesis of apple rootstock 54-118, it is better to use medium with 1/3 MS macro and microsalts, supplemented with IBA in concentration of 1.0 mg/l without etiolation (the yield of rooted microplants was 85.35 % with a coefficient of root system development – 0.53 ± 0.12). The positive effect of etiolation on root system growth depended on cultivar. For rootstock 54-118, the use of etiolation stimulated the growth of roots on medium with 1/3 MS macro- and microsalts combined with IBA in concentration of 1.0 mg/l (until the end of subculture). Use of etiolation inhibited the root system growth of rootstock 106-13 microplants until the end of subculture both on medium with 1/2 MS macro- and microsalts and on medium with 1/3 MS macro- and microsalts, but it stimulated root formation of rootstock 106-13. However, use of 7 days etiolation at stage of in vitro rhyzogenesis of rootstocks 106-13 and 54-118 is not expedient, because it does not stimulate the increase in the number of rooted microplants at the end of subculture.

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Optimization of microclonal propagation in vitro of oregano (Origanum vulgare)

Biosystems Diversity ◽

10.15421/011815 ◽

2018 ◽

Vol 26 (2) ◽

pp. 98-102 ◽

Cited By ~ 2

Author(s):

A. V. Fokina ◽

T. M. Satarova ◽

V. T. Smetanin ◽

N. I. Kucenko

Keyword(s):

Medicinal Plants ◽

Root System ◽

Root Formation ◽

System Development ◽

Practical Experience ◽

Shoot Length ◽

Herbaceous Plant ◽

Human Immune System ◽

Origanum Vulgare

Medicinal plants are important objects for botany, systematics and plant geography research, as well as physiology, pharmacology, and biotechnology. Medicinal plants from the Lamiaceae family are being intensively studied for medical and pharmacological reasons. This family also includes the medicinal herbaceous plant oregano (Origanum vulgare L.), known from ancient times for its antimicrobial and antifungal properties, the ability to strengthen the human immune system, and to improve the general state of an organism. At present, the study of its antimicrobial, antifungal, insecticidal, anticoagulant, antitumor, therapeutic and many other properties is being actively continued. Due to the relevance of the development of the principles of O. vulgare micropropagation in vitro and the undeveloped conditions and methods of its cultivation, the aim of this work was to optimize microclonation in vitro of oregano via the activation of auxiliary buds. The research tasks were to test the ability of auxiliary buds to be activated depending on the localization on the donor shoot internodes and to intensify the root formation of cuttings through medium content optimization. The influence of the location of the auxiliary buds on donor shoots on their activation in vitro was studied on such indicators as the length of newly formed shoots, the number of nodules per one newly formed shoot, and the number of newly formed shoots per one bud. In plant microclonal propagation, the stage of root formation is very important for further adaptation in soil. Practical experience has shown that for the effective adaptation of oregano in soil, the length of the root system for cuttings should be 1.5–2.0 cm, the degree of root system development – 4–5 points under shoot length of 3–5 cm. The study of peculiarities of oregano microclonal propagation via activation of auxiliary buds has allowed us to optimize the stage of explant selection for cutting and the formation of cuttings’ roots. It has been established that for optimal length, the number of nodules of the newly formed shoots and the number of newly formed shoots, the first internode, located on the top of a parent shoot, as well as the third to fifth ones are more suitable. For rooting oregano cuttings, the optimal medium on the ratio of length and density of root system and on shoot length is the nutrient one containing half of the macro-, microsalts and vitamins on Murashige-Skoog, 20 g/l sucrose and 0.75 mg/l kinetin.

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Timecourse Changes in Two Different Topological Indices with Seminal Root System Development of Rice.

Japanese Journal of Crop Science ◽

10.1626/jcs.65.303 ◽

1996 ◽

Vol 65 (2) ◽

pp. 303-308 ◽

Cited By ~ 3

Author(s):

Yasuhiro IZUMI ◽

Yasuhiro KONO ◽

Akira YAMAUCHI ◽

Morio IIJIMA

Keyword(s):

Root System ◽

System Development ◽

Topological Indices ◽

Seminal Root ◽

Root System Development

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The Effect of Salinity on Root Architecture in Forage Pea (Pisum sativum ssp. arvense L.)

Legume Research - An International Journal ◽

10.18805/lr-608 ◽

2021 ◽

Author(s):

S. Acikbas ◽

M.A. Ozyazici ◽

H. Bektas

Keyword(s):

Root System ◽

Root Architecture ◽

System Development ◽

Shoot Biomass ◽

Growth And Survival ◽

Legume Crop ◽

Root System Development ◽

Randomized Design ◽

Salinity Levels ◽

Root And Shoot Biomass

Background: Plants face different abiotic stresses such as salinity that affect their normal development, growth and survival. Forage pea is an important legume crop for herbage production in ruminants. Its agronomy requires high levels of irrigation and fertilization. This study aimed to evaluate the effect of salinity on seedling root system development in forage pea under semi-hydroponics conditions.Methods: Different treatment of NaCl doses (0, 50, 100, 150, 200, 250 and 300 mM) on root architecture was investigated in two different forage pea cultivars (Livioletta and Ulubatlý) with contrasting root structures under controlled conditions. The experimental design was completely randomized design with three replications and nine plants per replication.Result: Salinity affects root and shoot development differently on these cultivars. Despite the salinity, Livioletta produced more shoot (0.71 g) and root biomass (0.30 g) compared to Ulubatlý (0.52 g and 0.25 g for Root and Shoot biomass, respectively) at 150 mM and all other salinity levels. Livioletta developed a better root system and tolerated salt to a higher dose than Ulubatlý. Understanding root system responses of forage pea cultivars may allow breeding and selecting salinity tolerant cultivars with better rooting potential.

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Growth and development of Hippophae rhamnoides L. introduced in Absheron

NATURE AND SCIENCE ◽

10.36719/2707-1146/03/35-38 ◽

2020 ◽

Vol 02 (03) ◽

pp. 35-38

Author(s):

Kamala Arastun Sadigov ◽

Keyword(s):

Root System ◽

System Development ◽

Growth Dynamics ◽

Climatic Conditions ◽

Hippophae Rhamnoides ◽

Seedling Morphology ◽

Hippophaë Rhamnoides ◽

Root System Development ◽

Hippophae Rhamnoïdes L ◽

Seed Propagation

The presented article provides seed propagation, seedling morphology and growth dynamics, root system development in connection with the introduction of Hippophae rhamnoides L. species found in our natural flora in Absheron. The study found that the species Hippophae rhamnoides L. is well adapted to the soil and climatic conditions of Absheron and can be grown in cultural conditions. Key words: Hippophae rhamnoides L., introdiction, seed, repoduction, morphology, dewelopment, root system

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Root System Development of Juvenile Ponderosa Pine as Influenced by Soil Type and Nutritional Augmentation

Forest Research Open Access ◽

10.4172/2168-9776.1000187 ◽

2016 ◽

Vol 05 (04) ◽

Author(s):

Walker RF ◽

Susfalk RB ◽

Johnson DW

Keyword(s):

Root System ◽

Ponderosa Pine ◽

Soil Type ◽

System Development ◽

Root System Development

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Reaction of potential guava rootstocks to Meloidogyne enterolobii

Revista CERES ◽

10.1590/0034-737x201865030010 ◽

2018 ◽

Vol 65 (3) ◽

pp. 291-295 ◽

Cited By ~ 2

Author(s):

Fernando Marcelo Chiamolera ◽

Antonio Baldo Geraldo Martins ◽

Pedro Luiz Martins Soares ◽

Tatiana Pagan Loeiro da Cunha-Chiamolera

Keyword(s):

Root System ◽

Fusarium Solani ◽

Root Rot ◽

System Development ◽

Control Treatment ◽

Root Knot Nematode ◽

Second Stage ◽

Root System Development ◽

Meloidogyne Enterolobii ◽

Reproduction Factor

ABSTRACT Root-knot nematode Meloidogyne enterolobii is the main phytosanitary problem of guava cultivation in Brazil. Among the strategies to manage the problem, the best prospects are in identifying or developing cultivars or rootstocks that are resistant to this nematode. To identify plants with potential as rootstocks for guava, the reaction of araçá (wild guava) to M. enterolobii was assessed in a greenhouse experiment. Seven araçá species were evaluated (Eugenia stipitata, Psidium acutangulum, P. cattleyanum ‘yellow’, P. friedrichsthalianum, P. guajava var. minor, P. guineense, and Psidium sp.). The plants were inoculated with a suspension of 3,000 eggs of M. enterolobii, using eggplant as control treatment. The parameters fresh root mass, number of eggs and second stage juveniles (J2) per root system, the reproduction factor (RF = Pf/Pi), and araçá reaction were determined during the experiment. RF of the araçá species E. stipitata, P. cattleyanum ‘yellow’, and P. friedrichsthalianum was less than one (RP < 1), therefore resistant to M. enterolobii. The araçá trees had good root system development and the susceptible plants showed many root galls, high number of eggs and J2, and Fusarium solani and Rhizoctonia solani root rot. The araçá species, P. cattleyanum ‘yellow’, P. friedrichsthalianum, and E. stipitata are resistant to M. enterolobii and can be tested as potential guava rootstocks.

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Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v22.951 ◽

2018 ◽

Vol 22 ◽

pp. 216-221

Author(s):

O. V. Bulko ◽

L. G. Lioshina

Keyword(s):

In Vitro Culture ◽

Root Culture ◽

Medium Composition ◽

Cultivation Medium ◽

Plant Cultivation ◽

Regenerated Plants ◽

Composition Modification ◽

Jacob's Ladder ◽

Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

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