Effect of Different Levels of Vitamin C and Zinc on Sperm Quality Characteristics of Arabi Ram

We performed this experiment to evaluate the effects of adding vitamins C and E on extenders for sperm cryopreservation of Rhamdia quelen over spermatic mobility after thawing. At cryopreservation, sperm samples were diluted in a proportion of 1:3 (v/v), following pre-freezing in nitrogen steam and subsequent immersion in liquid nitrogen. The diluents were composed by 5% milk powder, 5% glucose, 10% methanol and different levels of vitamin. Three sperm cryopreservation tests were carried out with (1) diluent containing 0.0; 4.0; 6.5; 9.0 and 11.5 mg of vitamin C mL-1, (2) diluent containing 0.0; 2.0; 4.0; 6.0 and 8.0 mg of vitamin E mL-1; (3) diluent containing 0.0; 4.0 + 2.0; 6.5 + 4.0; 9.0 + 6.0 and 11.5 + 8.0 mg of vitamin C mL-1 plus vitamin E mL-1, respectively. The spermatic motility rate, spermatic curvilinear velocity, average path and straight line velocities were measured in thawed semen by CASA. Data were submitted to ANOVA and Duncan´s test at 5% of significance. After thawing the effect (P0.05) of vitamin C was observed only for sperm motility, with higher values (38.2±20.7%) on solution containing 4.0 mg of vitamin C mL-1. The concomitant addition of both vitamins influenced (P0.05) only the curvilinear velocity, reducing the velocity at any concentration. In conclusion, diluents with 4.0 mg vitamin C mL-1 to cryopreservation of the silver catfish semen improve the sperm quality after thawing, and the use of diluents with vitamin E or both vitamins are not recommended because do not ensure the cells protection.

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Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

Acta Veterinaria Scandinavica ◽

10.1186/s13028-021-00590-2 ◽

2021 ◽

Vol 63 (1) ◽

Author(s):

Maja Zakošek Pipan ◽

Petra Zrimšek ◽

Breda Jakovac Strajn ◽

Katarina Pavšič Vrtač ◽

Tanja Knific ◽

...

Keyword(s):

Seminal Plasma ◽

Semen Quality ◽

Sperm Quality ◽

Membrane Integrity ◽

Quality Characteristics ◽

Freezing And Thawing ◽

Additional Tool ◽

Bull Semen ◽

Bull Sperm ◽

Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

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Reproductive efficiency and egg and larvae quality of Nile tilapia fed different levels of vitamin C

Aquaculture ◽

10.1016/j.aquaculture.2017.08.035 ◽

2018 ◽

Vol 482 ◽

pp. 96-102 ◽

Cited By ~ 6

Author(s):

Nilda L.A.F. Sarmento ◽

Edenilce F.F. Martins ◽

Deliane C. Costa ◽

Cristiano C. Mattioli ◽

Gustavo S. da Costa Julio ◽

...

Keyword(s):

Vitamin C ◽

Nile Tilapia ◽

Reproductive Efficiency ◽

Different Levels

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The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

World Nutrition Journal ◽

10.25220/wnj.v01i1.0007 ◽

2017 ◽

Vol 1 (1) ◽

pp. 28

Author(s):

Eviana Budiartanti Sutanto ◽

Taufiq R Nasihun ◽

Israhnanto Isradji ◽

Luciana Budiati Sutanto

Keyword(s):

Vitamin C ◽

Sperm Quality ◽

Sperm Concentration ◽

Control Group ◽

Control Group Design ◽

Normal Sperm ◽

Post Test ◽

Male Wistar Rats ◽

Post Hoc ◽

Per Oral

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in K2 (K2 32.59 million/mL, K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

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