Extenders with vitamins C and E applied to Rhamdia quelen sperm cryopreservation  / Extensores com vitaminas C e E aplicados à criopreservação de esperma de Rhamdia quelen

We performed this experiment to evaluate the effects of adding vitamins C and E on extenders for sperm cryopreservation of Rhamdia quelen over spermatic mobility after thawing. At cryopreservation, sperm samples were diluted in a proportion of 1:3 (v/v), following pre-freezing in nitrogen steam and subsequent immersion in liquid nitrogen. The diluents were composed by 5% milk powder, 5% glucose, 10% methanol and different levels of vitamin. Three sperm cryopreservation tests were carried out with (1) diluent containing 0.0; 4.0; 6.5; 9.0 and 11.5 mg of vitamin C mL-1, (2) diluent containing 0.0; 2.0; 4.0; 6.0 and 8.0 mg of vitamin E mL-1; (3) diluent containing 0.0; 4.0 + 2.0; 6.5 + 4.0; 9.0 + 6.0 and 11.5 + 8.0 mg of vitamin C mL-1 plus vitamin E mL-1, respectively. The spermatic motility rate, spermatic curvilinear velocity, average path and straight line velocities were measured in thawed semen by CASA. Data were submitted to ANOVA and Duncan´s test at 5% of significance. After thawing the effect (P0.05) of vitamin C was observed only for sperm motility, with higher values (38.2±20.7%) on solution containing 4.0 mg of vitamin C mL-1. The concomitant addition of both vitamins influenced (P0.05) only the curvilinear velocity, reducing the velocity at any concentration. In conclusion, diluents with 4.0 mg vitamin C mL-1 to cryopreservation of the silver catfish semen improve the sperm quality after thawing, and the use of diluents with vitamin E or both vitamins are not recommended because do not ensure the cells protection.

Download Full-text

The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality

Cell and Tissue Banking ◽

10.1007/s10561-015-9506-9 ◽

2015 ◽

Vol 16 (4) ◽

pp. 587-592 ◽

Cited By ~ 21

Author(s):

Mahmood Reza Amini ◽

Hamid Kohram ◽

Ahmad Zare Shahaneh ◽

Mahdi Zhandi ◽

Hossein Sharideh ◽

...

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Sperm Quality ◽

Different Levels

Download Full-text

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

Brazilian Journal of Biology ◽

10.1590/1519-6984.20613 ◽

2015 ◽

Vol 75 (3) ◽

pp. 662-669 ◽

Cited By ~ 2

Author(s):

EG Sanches ◽

IR Oliveira ◽

PCS Serralheiro ◽

VR Cerqueira

Keyword(s):

Sperm Motility ◽

Sperm Quality ◽

Equilibration Time ◽

Dilution Ratio ◽

Sperm Cryopreservation ◽

Ph Values ◽

Cryopreserved Sperm ◽

Time Density ◽

Motility Rate

AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.

Download Full-text

Effects of anesthetic tricaine on stress and reproductive aspects of South American silver catfish (Rhamdia quelen) male

10.1101/759340 ◽

2019 ◽

Author(s):

Nathalia dos Santos Teixeira ◽

Lis Santos Marques ◽

Rômulo Batista Rodrigues ◽

Darlan Gusso ◽

Ana Amélia Nunes Fossati ◽

...

Keyword(s):

Induction Time ◽

Plasma Cortisol ◽

Sperm Quality ◽

Reproductive Hormones ◽

Anesthesia Induction ◽

South American ◽

Silver Catfish ◽

Rhamdia Quelen ◽

Cortisol Levels ◽

Motility Rate

AbstractAnesthesia is a common practice used in fish research and aquaculture. For both applications, it is important to understand anesthetics effects on the animal and tissues of interest to ensure the validity of data and to improve animal welfare. Captive fish production is only possible with artificial reproduction, and it is known that manipulation is a stressor stimulus in fish. The most common method of determining fish stress responses is measuring the circulating level of cortisol. Therefore, the purpose of this study was to evaluate the effects of different concentrations (100, 200, and 300 mg L-1) of the anesthetic tricaine methanesulfonate (MS-222) on cortisol levels and their influence on the sperm quality maintenance in Rhamdia quelen. After hormonal induction, 28 sexually mature males (average weight = 363.00 ± 71.24 g) were randomly distributed among treatments, and their semen and blood samples were collected. Anesthesia induction time, motility rate, sperm concentration and morphology, plasma cortisol levels, and reproductive hormones concentrations (testosterone, 17-α-hydroxyprogesterone, and estradiol) were evaluated. Anesthesia with 100 mg L-1 MS-222 presented a longer induction time than that with 200 and 300 mg L-1 MS-222. Sperm motility rate was significantly higher in the control than in the 300 mg L-1 treatment but did not differ among the control, 100, and 200 mg L-1 treatments. Estradiol level was significantly higher in non-anesthetized than in anesthetized fish, but plasma cortisol levels did not differ significantly between treatments (182.50 ± 42.03 ng mL-1). MS-222 anesthetizes fish by blocking the sodium channels, preventing the development of nerve action potentials. However, MS222 at concentrations of 100, 200, and 300 mg L-1 did not prevent stress in South American silver catfish males. In addition, its use did not maintain sperm quality, as it impaired motility and decreased levels of plasma estradiol.

Download Full-text

Effect of Astaxanthin, Vitamin E, and Vitamin C in Combination with Calorie Restriction on Sperm Quality and Quantity in Male Rats

Journal of Dietary Supplements ◽

10.1080/19390211.2016.1211783 ◽

2016 ◽

Vol 14 (3) ◽

pp. 252-263 ◽

Cited By ~ 6

Author(s):

Aliasghar Vahidinia ◽

Ali Reza Rahbar ◽

Mohammad Mehdi Shakoori Mahmoodabadi

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Calorie Restriction ◽

Sperm Quality ◽

Male Rats

Download Full-text

Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm

Neotropical Ichthyology ◽

10.1590/1982-0224-20130207 ◽

2014 ◽

Vol 12 (3) ◽

pp. 643-648 ◽

Cited By ~ 7

Author(s):

Ana T. M. Viveiros ◽

Tais R. Taffarel ◽

Marcelo C. Leal

Keyword(s):

Cold Storage ◽

Sperm Quality ◽

The Other ◽

Coconut Water ◽

Prochilodus Lineatus ◽

Straight Line ◽

And Control ◽

Motility Rate

This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatussperm. Sperm was diluted in six extenders: two compositions (powdered coconut water(tm) = ACP; Beltsville Thawing Solution(tm) = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatussperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.

Download Full-text

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2017-0011 ◽

2017 ◽

Vol 20 (1) ◽

pp. 77-84 ◽

Cited By ~ 4

Author(s):

A. Dziekońska ◽

K. Świąder ◽

M. Koziorowska-Gilun ◽

K. Mietelska ◽

Ł. Zasiadczyk ◽

...

Keyword(s):

Sperm Quality ◽

Membrane Integrity ◽

Quality Parameters ◽

Anova Analysis ◽

Short Term ◽

Straight Line ◽

Boar Spermatozoa ◽

Velocity Average

AbstractThe aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17°C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0.05) were noted between F1 and F2 in progressive motility (PMOT), velocity average pathway (VAP) and velocity straight line (VCL). After the ejaculate was diluted, the type of fraction and type of extender significantly affected (p<0.05) PMOT, being markedly higher (p<0.05) for F1 extended in BTS. No marked changes (p<0.05) were observed between F1 and F2 extended in SCP for any of the analyzed sperm quality parameters during seven days of storage. Significantly higher (p<0.05) values of sperm quality parameters were noted in F1 compared with F2 for BTS on D7 of storage. The results of the four-way ANOVA analysis indicate that boar, fraction of ejaculate, extender type and day of storage had significant effects on the quality of boar stored spermatozoa. The F1 was characterised by higher quality of spermatozoa during storage in comparison with F2 in the short-term extender. Using the long-term extender containing the proteins allowed for a better application of F2, which could be important for the pig industry.

Download Full-text

Sperm dilution ratio affects post-thaw motility rate and velocity of Prochilodus lineatus (Characiformes) sperm

Zygote ◽

10.1017/s0967199415000635 ◽

2015 ◽

Vol 24 (5) ◽

pp. 662-667 ◽

Cited By ~ 4

Author(s):

Ana T.M. Viveiros ◽

Marcelo C. Leal

Keyword(s):

Sperm Quality ◽

Aquatic Organisms ◽

Computer Assisted ◽

Dilution Ratio ◽

Prochilodus Lineatus ◽

Freezing Medium ◽

Final Volume ◽

Straight Line ◽

Motility Rate

SummaryThere is a lack of standardization in sperm cryopreservation of aquatic organisms and, thus, a necessity of more accurate investigations in all steps of this process. In this study, the effects of sperm dilution ratio on post-thaw sperm quality of Prochilodus lineatus were evaluated. Sperm was diluted in a standard freezing medium (glucose and methyl glycol) at four different ratios (sperm to final volume = 1:5, 1:10, 1:50 or 1:100), frozen in a nitrogen vapour vessel at –170°C and then stored in liquid nitrogen vessel at –196°C. Post-thaw motility rate and velocities (curvilinear = VCL; average path = VAP; straight line = VSL) were determined using a Computer-Assisted Sperm Analyzer (CASA) at 10 and 40 s post-activation. The highest motility rates were observed when sperm was frozen at a ratio of 1:5 (76%) and 1:10 (75%). The highest VCL (225 μm/s) and VAP (203 μm/s) were observed at a ratio of 1:10, while VSL was similar among samples frozen at 1:5, 1:10 and 1:50 (97–124 μm/s). When those parameters were evaluated again 30 s later, motility decreased significantly in samples frozen at a ratio of 1:5 (57%) and 1:10 (61%), while velocities decreased significantly in all samples regardless of dilution ratio (75–85 μm/s of VCL, 38–53 μm/s of VAP and 25–39 μm/s of VSL). P. lineatus sperm should be frozen at a ratio of 1:10, where both the number of loaded sperm per straw and the post-thaw quality are maximized.

Download Full-text

Modulatory activity of antioxidants against the toxicity of Rifampicin in vivo

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652010000100007 ◽

2010 ◽

Vol 52 (1) ◽

pp. 43-46 ◽

Cited By ~ 26

Author(s):

Olufunsho Awodele ◽

Alade Akintonwa ◽

Vincent O. Osunkalu ◽

Herbert A.B. Coker

Keyword(s):

Vitamin E ◽

Liver Function ◽

Vitamin C ◽

Histopathological Examination ◽

Sperm Quality ◽

Clinical Chemistry ◽

Treated Group ◽

World Health ◽

Albino Rats ◽

Therapeutic Doses

The World Health Organization (WHO) has shown concern about the burden of tuberculosis in the developing countries. Even though rifampicin is an effective drug in the management of tuberculosis, it has been documented to have some toxic effects in humans. Therefore, this study intends to investigate the modulatory effect of vitamins C and E on the hepatotoxicity, sperm quality and brain toxicity of Rifampicin. Forty Wistar albino rats were used, 10 animals per group. Group 1 animals received 0.3 mL of distilled water, the Group 2 animals received the therapeutic dose of rifampicin, Group 3 animals received therapeutic doses of rifampicin plus vitamin E, while Group 4 received therapeutic doses of rifampicin and vitamin C. The administration was performed orally during three months; the animals were sacrificed by cervical dislocation at the end of that period. Blood samples were collected and liver function and lipid profile was analyzed using fully automated clinical chemistry device. The liver, brain and reproductive organs underwent histopathological examination. Sperm samples were collected from the epididymis to achieve count and motility and morphological analysis. Results showed rifampicin alone to raise (p < 0.05) liver function enzymes (Aspartate amino transferase [AST], Serum alanine amino transferase [ALT] and Total Bilirubin) when compared with controls. While the vitamin E treated group showed remarkable protection, the vitamin C treated group showed questionable protection against the rifampicin induced liver damage. Sperm count results showed an important (p < 0.05) increase in the sperm quality in vitamin E and C treated groups. However, the vitamin E plus Rifampicin treated group showed increased lipid peroxidation. The histopathological findings revealed structural damages by rifampicin in liver, brain and epididymis while some remarkable architectural integrity was observed in the antioxidant-treated groups. It can be concluded that vitamin E or C improved sperm quality and protected against the brain damage caused by rifampicin. Moreover, vitamin E demonstrated remarkable hepatoprotection against rifampicin induced damage while vitamin C shows a questionable hepatoprotection.

Download Full-text