scholarly journals Effects of Different Concentrations of L-Carnitine in Lecithin-Based Semen Extender on Semen Quality of Ghezel Ram after Freeze Thawing Process

2018 ◽  
Vol 9 (19) ◽  
pp. 48-53
Author(s):  
Hossein Daghigh Kia ◽  
Sepehr Jafari ◽  
Gholamali Moghaddam ◽  
Marziyeh Ebrahimi ◽  
Abozar Najafi ◽  
...  
Keyword(s):  
Semen Quality ◽  
Thawing Process ◽  
Semen Extender

scholarly journals Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein

2019 ◽  
Vol 20 (1) ◽  
pp. 140
Author(s):  
Muttaqinullah Rabusin ◽  
Andriani Andriani ◽  
Raden Iis Arifiantini ◽  
Ni Wayan Kurniani Karja
Keyword(s):  
Semen Quality ◽  
Bacterial Species ◽  
Enterobacter Cloacae ◽  
Bovine Semen ◽  
Frozen Semen ◽  
Bacterial Content ◽  
Pseudomonas Diminuta ◽  
Semen Extender ◽  
Fresh Semen

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen.  For this purpose, three experiments were conducted.  In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates.  In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen.  In the result, some of bacterial species were isolated from the bovine semen.  The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa.  Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp..  Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica.  Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica.  In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen. 

PSV-9 Effects of Natural Honey Inclusion in Dilution and Freezing Extenders on Frozen-thawed Semen Quality in Boars

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 211-211
Author(s):  
Kayode B Balogun ◽  
Griffin Nicholls ◽  
Olujide Sokunbi ◽  
Kara R Stewart
Keyword(s):  
Semen Quality ◽  
Swine Industry ◽  
Normal Cells ◽  
Progressive Motility ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Boar Semen ◽  
Semen Extender ◽  
Natural Honey

Abstract Improvements in the post-thaw quality of frozen semen could allow increased utilization of this technology in the swine industry. The objective of this study was to investigate the effects of natural honey inclusion in semen extender and freezing media on motility, mobility, and morphology of cryopreserved boar sperm. Ejaculates from 6 terminal cross-bred boars were collected using the gloved-hand technique for 3 weeks and used in a 2 x 3 factorial study design. Following collection, semen samples were incubated overnight in dilution extender with and without natural honey (D0: Androhep Plus; D1: Androhep Plus + 0.25% honey). The following day, the semen samples were cooled to 4 C in LEY cooling extender then frozen in freezing media containing 93% cooling extender + 6% glycerol + 1% Equex-STM Paste (F1), or freezing media with natural honey replacing 50% of the glycerol (F2) or 50% of the Equex-STM paste (F3). Semen samples were frozen using a controlled-rate freezer and stored in liquid nitrogen. Two straws per treatment for each boar were thawed and semen quality assessed. The inclusion of natural honey in dilution extender had no effect on post-thaw motility (P=0.733), progressive motility (P=0.562), or other mobility parameters (0.995≤P≥0.081). However, D1 had a higher percentage of normal acrosomes (P=0.001) and morphologically normal cells (P< 0.001) resulting from lower tail abnormalities compared to D0 (P=0.006). Post-thaw motility (P< 0.001) and progressive motility (P< 0.001) were increased in F3 compared to both F2 and F1. F1 had reduced normal acrosomes (P=0.009) and morphologically normal cells (P< 0.001) resulting from higher tail abnormalities (P< 0.001). In conclusion, the inclusion of natural honey, at 0.25%, in dilution extender helps maintain normal sperm and acrosome morphology, and replacing 50% Equex-STM Paste with honey in freezing extender improves post-thaw sperm motility and progressive motility of frozen-thawed boar semen.

Reduction of dissolved oxygen in semen extender with nitrogen gassing reduces oxidative stress and improves post-thaw semen quality of bulls

2018 ◽  
Vol 197 ◽  
pp. 162-169 ◽  
Author(s):  
Abhishek Kumar ◽  
J.K. Prasad ◽  
A.R. Mustapha ◽  
Beigh Yaqoob Amin ◽  
Omer Din ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dissolved Oxygen ◽  
Semen Quality ◽  
Semen Extender

scholarly journals Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality

2020 ◽  
Vol 142 ◽  
pp. 02002
Author(s):  
Fariz Zharfan Haris ◽  
Yon Soepri Ondho ◽  
Daud Samsudewa
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
Freezing Process ◽  
Bull Semen ◽  
Sperm Abnormality ◽  
Thawing Process ◽  
T1 And T2 ◽  
Semen Extender ◽  
Mortality Percentage

The purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. The material used for research were semen from two selected Simmental bulls. Vitamin E as treatment in addition to the extender consisted of T0 (no addition of vitamin E), T1 (0,134 gram/100 ml extender), T2 (0,268 gram/100 ml extender) and T3 (0,402 gram/100 ml extender). Post-thawing evaluation conducted 24 hours after the freezing process and observed after the thawing process in 37°C water bath for 30 seconds. Parameters observed in this research were the post-thawing quality of frozen-thawed Simmental bull semen based on motility, mortality and abnormality percentage. Sperm motility evaluated using a microscope with 100x and 400x magnifying, sperm mortality observed using 400x magnifying and counted based on 0,2% eosin-negrosin staining, sperm abnormality observed using 400x magnifying and counted based on the number of morphologically abnormal sperm cells. Semen post-thawing motility was not significantly affected by vitamin E addition of T1, T2 and T3 (P<0,05). T1 and T2 were able to significantly lower the mortality percentage compared to T0 and T3 (P<0,05). T1 and T2 were also very significantly affecting the decreased of abnormality percentage compared to T0 and T3 (P<0,01). T1 generally gave the best result on the improvement of post-thawing motility and decreased the percentage of sperm mortality and abnormality.

scholarly journals  Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 377-381 ◽  
Author(s):  
M. Ziaullah ◽  
A. Ijaz ◽  
M. Aleem ◽  
A.K. Mahmood ◽  
H. Rahman ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Butylated Hydroxytoluene ◽  
Osmotic Swelling ◽  
Inclusion Level ◽  
Semen Extender ◽  
Supravital Staining

The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at &minus;196&deg;C using 200 &times; 10<sup>6 </sup>spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P &gt; 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects. &nbsp;

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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