scholarly journals IMMUNOHISTOCHEMICAL ANALYSIS OF CATHEPSIN K AND OSTEOPONTIN EXPRESSION BY CELLULAR ELEMENTS OF REGENERATED BONE TISSUE UNDER THE INFLUENCE OF ANTITUMOR CHEMOTHERAPEUTICS

2021 ◽  
Vol 2 (1) ◽  
pp. 236
Author(s):  
T. V. Riabenko ◽  
O. V. Korenkov ◽  
A. A. Ponyrko ◽  
T. P. Teslyk ◽  
V. I. Hula
Keyword(s):  
Bone Tissue ◽  
Cathepsin K ◽  
Immunohistochemical Analysis ◽  
Osteopontin Expression

scholarly journals Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats

2018 ◽  
Vol 19 (11) ◽  
pp. 3488 ◽  
Author(s):  
Aline Pereira ◽  
Gerly Brito ◽  
Maria Lima ◽  
Arnóbio Silva Júnior ◽  
Emanuell Silva ◽  
...  
Keyword(s):  
Bone Loss ◽  
Cathepsin K ◽  
Immunohistochemical Analysis ◽  
Entrapment Efficiency ◽  
Inflammatory Cells ◽  
Plga Nanoparticles ◽  
Diabetic Rats ◽  
Metformin Hydrochloride ◽  
Tnf Α ◽  
Mean Diameter

Evidence shows that metformin is an antidiabetic drug, which can exert favorable anti-inflammatory effects and decreased bone loss. The development of nanoparticles for metformin might be useful for increased therapeutic efficacy. The aim of this study was to evaluate the effect of metformin hydrochloride-loaded Poly (d,l-Lactide-co-glycolide) (PLGA)/(MET-loaded PLGA) on a ligature-induced periodontitis model in diabetic rats. MET-loaded PLGA were characterized by mean diameter, particle size, polydispensity index, and entrapment efficiency. Maxillae were scanned using Microcomputed Tomography (µCT) and histopathological and immunohistochemical analysis. IL-1β and TNF-α levels were analyzed by ELISA immunoassay. Quantitative RT-PCR was used (AMPK, NF-κB p65, HMGB1, and TAK-1). The mean diameter of MET-loaded PLGA nanoparticles was in a range of 457.1 ± 48.9 nm (p < 0.05) with a polydispersity index of 0.285 (p < 0.05), Z potential of 8.16 ± 1.1 mV (p < 0.01), and entrapment efficiency (EE) of 66.7 ± 3.73. Treatment with MET-loaded PLGA 10 mg/kg showed low inflammatory cells, weak staining by RANKL, cathepsin K, OPG, and osteocalcin, and levels of IL-1β and TNF-α (p < 0.05), increased AMPK expression gene (p < 0.05) and decreased NF-κB p65, HMGB1, and TAK-1 (p < 0.05). It is concluded that MET-loaded PLGA decreased inflammation and bone loss in periodontitis in diabetic rats.

Cathepsin K and matrix metalloprotease activities in bone tissue of the OXYS rats during the development of osteoporosis

2009 ◽  
Vol 3 (4) ◽  
pp. 393-398
Author(s):  
A. A. Venediktova ◽  
O. V. Falameeva ◽  
N. G. Kolosova ◽  
M. A. Sadovoj ◽  
T. A. Korolenko
Keyword(s):  
Bone Tissue ◽  
Cathepsin K ◽  
Oxys Rats ◽  
Matrix Metalloprotease

scholarly journals Modulation of Osteonectin and Osteopontin Expression during Fracture Healing of Mouse Bone Tissue.

1998 ◽  
Vol 31 (6) ◽  
pp. 501-508 ◽  
Author(s):  
Hideyoshi Kashimoto ◽  
Riko Kitazawa ◽  
Sakan Maeda ◽  
Kosaku Mizuno ◽  
Sohei Kitazawa
Keyword(s):  
Fracture Healing ◽  
Bone Tissue ◽  
Osteopontin Expression

scholarly journals Immunohistochemical Analysis Revealed the Expression of Bone Morphogenetic Proteins-4, 6, 7, and 9 in Human Induced Membrane Samples Treated With the Masquelet Technique

2021 ◽  
Author(s):  
Takahiro Niikura ◽  
Takahiro Oda ◽  
Naoe Jimbo ◽  
Masato Komatsu ◽  
Keisuke Oe ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Morphogenetic Proteins ◽  
Immunohistochemical Analysis ◽  
Bone Defects ◽  
Positive Control ◽  
Cement Spacer ◽  
Induced Membrane ◽  
Masquelet Technique ◽  
Human Bone Tissue ◽  
Large Bone

Abstract BackgroundInduced membrane (IM) is the key component of Masquelet reconstruction surgery for the treatment of bone defects. It is formed around the cement spacer and is known to secrete growth factors and osteoinductive factors. However, information on the presence of osteoinductive factors in IM is not enough in the literature. The purpose of this study was to investigate the existence of bone morphogenetic proteins (BMPs) in the IM harvested from patients during the treatment of bone defects using the Masquelet technique.MethodsWe included six patients whose bone defects were treated using the Masquelet technique. The affected bone was the femur in three patients and the tibia in three patients. During the second-stage surgery, 1-cm2 pieces of IM were harvested. Histological sections of IM were immunostained with anti-BMP-4, 6, 7, and 9 antibodies. Human bone tissue served as the positive control.ResultsThe existence of BMP-4, 6, 7, and 9 was observed in all IM samples. Further, immunolocalization of BMP-4, 6, 7, and 9 was observed in blood vessels and fibroblasts of all IM samples. Immunolocalization of BMP-4, 6, 7, and 9 was also observed in bone tissue within the IM in one sample, in which osteogenesis inside the IM was observed.ConclusionsThis study revealed that osteoinductive factors BMP-4, 6, 7, and 9 were present in the IM harvested from patients. This helps explain how the Masquelet technique effectively contributes to the healing of large bone defects. It may thus be possible for surgeons to omit the addition of BMPs to bone grafts give the endogenous secretion of BMPs from the IM.Trial registrationNot applicable.

scholarly journals Cathepsin K and matrix metalloproteases activity in bone tissue of OXYS rats with osteoporosis development

2010 ◽  
Vol 56 (2) ◽  
pp. 274-282 ◽  
Author(s):  
A.A. Venediktova ◽  
O.V. Falameeva ◽  
N.G. Kolosova ◽  
M.A. Sadovoj ◽  
T.A. Korolenko
Keyword(s):  
Bone Tissue ◽  
Wistar Rats ◽  
Cathepsin K ◽  
Typical Feature ◽  
Matrix Metalloproteases ◽  
Oxys Rats ◽  
Age Related ◽  
Α2 Macroglobulin ◽  
The Comparative Study ◽  
K Activity

The comparative study of аctivity of cysteine protease cathepsin K and matrix metalloproteases (MMPs) in bone tissue of accelerated senescent OXYS rats with early ageing comparatively to Wistar rats of the same age was performed. Early development osteoporosis is a typical feature of OXYS rats. In bone tissue of 3 month old OXYS rats, before appearance of osteoporosis manifestation cathepsin K activity was higher, whereas MMPs activity was lower than in Wistar rats. In Wistar rats (3 and 14 months old) cathepsin K activity of spine was shown to increase, and MMPs activity to decrease. In OXYS rats age-related change of cathepsin K and MMPs activity in bone tissue had the opposite direction. As a result of this there were no differences between Wistar and OXYS rats 14 months old despite the marked osteoporosis in OXYS rats as revealed our early researches. Serum α2-macroglobulin activity was higher in 14 months old OXYS rats. The role of activation of cathepsin K in bone resorption in the development of osteoporosis in early ageing OXYS rats is discussed.

scholarly journals Immunohistochemical analysis revealed the expression of bone morphogenetic proteins-4, 6, 7, and 9 in human induced membrane samples treated with the Masquelet technique

2022 ◽  
Vol 17 (1) ◽  
Author(s):  
Takahiro Niikura ◽  
Takahiro Oda ◽  
Naoe Jimbo ◽  
Masato Komatsu ◽  
Keisuke Oe ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Morphogenetic Proteins ◽  
Immunohistochemical Analysis ◽  
Bone Defects ◽  
Positive Control ◽  
Cement Spacer ◽  
Induced Membrane ◽  
Masquelet Technique ◽  
Human Bone Tissue ◽  
Large Bone

Abstract Background Induced membrane (IM) is the key component of Masquelet reconstruction surgery for the treatment of bone defects. IM is formed around the cement spacer and is known to secrete growth factors and osteoinductive factors. However, there is limited evidence available concerning the presence of osteoinductive factors in IM. This study aimed to investigate the existence of bone morphogenetic proteins (BMPs) in IM harvested from patients during the treatment of bone defects using the Masquelet technique. Methods This study involved six patients whose bone defects had been treated using the Masquelet technique. The affected sites were the femur (n = 3) and the tibia (n = 3). During the second-stage surgery, 1 cm2 pieces of IM were harvested. Histological sections of IM were immunostained with anti-BMP-4, 6, 7, and 9 antibodies. Human bone tissue served as the positive control. Results The presence of BMP-4, 6, 7, and 9 was observed in all IM samples. Further, immunolocalization of BMP-4, 6, 7, and 9 was observed in blood vessels and fibroblasts in all IM samples. Immunolocalization of BMP-4, 6, 7, and 9 was also observed in bone tissue within the IM in one sample, in which osteogenesis inside the IM was observed. Conclusions This study showed that osteoinductive factors BMP-4, 6, 7, and 9 were present in the IM harvested from patients, providing evidence indicating that the Masquelet technique effectively contributes to healing large bone defects. Therefore, it may be possible for surgeons to omit the addition of BMPs to bone grafts, given the endogenous secretion of BMPs from the IM.

scholarly journals The impact of harmful occupational risk factors on the markers of bone metabolism in industrial workers

2021 ◽  
Vol 100 (9) ◽  
pp. 964-968
Author(s):  
Kristina V. Klimkina ◽  
Inna V. Lapko
Keyword(s):  
Bone Metabolism ◽  
Bone Tissue ◽  
Cathepsin K ◽  
Phosphorus Metabolism ◽  
Industrial Workers ◽  
Mineral Density ◽  
Occupational Risk Factors ◽  
Industrial Enterprises ◽  
Calcium Phosphorus ◽  
The Impact

Introduction. Unfavourable factors of the working environment affect bone tissue, increasing the risk of developing osteoporosis in workers of harmful industries, which is an urgent social and hygienic problem due to the high prevalence and severity of medical and social consequences. The aim of the study was to investigate the impact of industrial vibration and physical overload on the markers of bone metabolism in industrial workers. Materials and methods. One hundred fifty-two employees of mining and mechanical engineering enterprises in contact with industrial vibration and physical overload were examined. The assessment of bone tissue metabolism in the workers was carried out by the method of solid-phase enzyme immunoassay to determine biochemical markers of bone metabolism: bone alkaline phosphatase, osteocalcin, C-terminal serum telopeptide, cathepsin K, as well as indicators of calcium-phosphorus metabolism. X-ray densitometry was used to assess bone mineral density. Results. There was a significant decrease in the markers of osteogenesis: the concentration of CSF (14.26 ± 4.13 and 18.69±5.28, respectively, p< 0.05) and osteocalcin (12.23±4.15 and 17.84±5.17, respectively, p < 0.05) and an increase in the markers of bone resorption: the level of beta-CrossLaps (0.629±0.162 and 0.361±0.095, respectively, p = 0.002) and the level of cathepsin K (12.49±5.28 and 0.59±0.02, respectively, p < 0.001) in workers of industrial enterprises exposed to physical factors of production. The correlation between the markers of bone metabolism and the experience of exposure to adverse working conditions was established. Conclusion. Combined exposure to physical labour factors is a predictor of calcium-phosphorus metabolism and bone metabolism disorders, increasing the risk of metabolic osteopathy in industrial workers.

Expression of cathepsin K is regulated by shear stress in cultured endothelial cells and is increased in endothelium in human atherosclerosis

2007 ◽  
Vol 292 (3) ◽  
pp. H1479-H1486 ◽  
Author(s):  
Manu O. Platt ◽  
Randall F. Ankeny ◽  
Guo-Ping Shi ◽  
Daiana Weiss ◽  
J. D. Vega ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Molecular Mechanisms ◽  
Cathepsin K ◽  
Immunohistochemical Analysis ◽  
Cysteine Proteases ◽  
Laminar Shear Stress ◽  
Gelatinase Activity ◽  
Aortic Endothelial Cells ◽  
Expression And Activity

Cathepsins, the lysosomal cysteine proteases, are involved in vascular remodeling and atherosclerosis. Genetic knockout of cathepsins S and K in mice has shown to reduce atherosclerosis, although the molecular mechanisms remain unclear. Because atherosclerosis preferentially occurs in arteries exposed to disturbed flow conditions, we hypothesized that shear stress would regulate cathepsin K expression and activity in endothelial cells. Mouse aortic endothelial cells (MAEC) exposed to proatherogenic oscillatory shear (OS, ± 5 dyn/cm2 for 1 day) showed significantly higher cathepsin K expression and activity than that of atheroprotective, unidirectional laminar shear stress (LS, 15 dyn/cm2 for 1 day). Western blot and active-site labeling studies showed an active, mature form of cathepsin K in the conditioned medium of MAEC exposed to OS but not in that of LS. Functionally, MAEC exposed to OS significantly increased elastase and gelatinase activity above that of LS. The OS-dependent elastase and gelatinase activities were significantly reduced by knocking down cathepsin K with small-interfering (si) RNA, but not by a nonsilencing siRNA control, suggesting that cathepsin K is a shear-sensitive protease. In addition, immunohistochemical analysis of atherosclerotic human coronary arteries showed a positive correlation between the cathepsin K expression levels in endothelium and elastic lamina integrity. These findings suggest that cathepsin K is a mechanosensitive, extracellular matrix protease that, in turn, may be involved in arterial wall remodeling and atherosclerosis.

An Intrinsic Angiogenesis Approach and Varying Bioceramic Scaffold Architecture Affect Blood Vessel Formation in Bone Tissue Engineering In Vivo

2016 ◽  
Vol 720 ◽  
pp. 58-64 ◽  
Author(s):  
Doaa Adel-Khattab ◽  
Marian Kampsculte ◽  
Barbara Peleska ◽  
Renate Gildenhaar ◽  
Georg Berger ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Immunohistochemical Analysis ◽  
Dynamic Cell ◽  
Volume Blood ◽  
Av Bundle

Early establishment of angiogenesis is critical for bone tissue engineering. Recently, a technique was introduced, which is based on the idea of using axial vascularization of the host tissues in engineered grafts, namely the “intrinsic angiogenesis chamber” technique, which utilizes an artery and a vein to construct an AV-Bundle. The aim of this study was to evaluate the effect of varying scaffold architecture of calcium alkali orthophosphate scaffolds (CAOP), resulting from two different fabrication procedures, namely 3D printing (RP) or a Schwarzwalder-Somers replica technique (SSM), on angiogenesis in vivo when combining a microvascular technique with bioceramic scaffolds colonized with stem cells for bone tissue engineering. 32 adult female Wistar rats, in which critical size segmental discontinuity defects 6 mm in length were created in the left femur, were divided into 4 groups, group 1 received a RP scaffold colonized with rat stem cells after 7d of dynamic cell culture and an AV-Bundle (AVB), group 2 a SSM scaffold with rat stem cells after 7d of dynamic cell culture and an AVB, group 3 a RP control scaffold (without cells and AVB), group 4 a SSM control scaffold (without cells and AVB). After 3 and 6 months, angiomicro-CT after perfusion with a contrast agent, image reconstruction, histomorphometric and immunohistochemical analysis utilizing antibodies to collagen IV, vWF and CD-31 were performed. At 6 months, a statistically significant higher blood vessel volume%, blood vessel surface/volume, blood vessel thickness, blood vessel density and blood vessel linear density was observed with RP scaffolds with cells and AVB than with the other groups. At 6 mths, RP with cells and AVB displayed the highest expression of collagen IV (score 2.75), CD31 (score 2.75) and vWF (score 2.6), which is indicative of highly dense blood vessels. Both angio-CT and immunohistochemical analysis demonstrated that AVB is an efficient technique for achieving scaffold vascularization in critical size segmental defects after 3 and 6 months of implantation.

Sign in / Sign up

Export Citation Format

Share Document