scholarly journals Evaluación del perfil de nutrientes de bagazo de agave como alternativa de alimento para rumiantes

2018 ◽  
pp. 2099
Author(s):  
Lucia Delgadillo Ruíz ◽  
Rómulo Bañuelos Valenzuela ◽  
Edgar León Esparza Ibarra ◽  
Héctor Gutiérrez Bañuelos ◽  
Francisco Javier Cabral Arellano ◽  
...  
Keyword(s):  
Agave Salmiana

El objetivo de este trabajo fue evaluar el perfil in vitro de nutrientes de bagazo de Agave salmiana (AS) y Agave weberi cela (AWC) como alternativa para la nutrición de los rumiantes. ElbagazoserecogióenJalpa,Zacatecasduranteelaño2010.A partir de las muestras recogidas, se evaluó: materia seca (MS), cenizas, fibra cruda (FC), extracto etéreo (EE), proteína cruda (PC), carbono orgánico total (TOC), detergente neutro de f ibra (DNF), y azúcares reductores totales (ART). Macro y micro- minerales se determinaron por espectroscopia de absorción atómica.Además, se utilizó una técnica de producción de gas in vitro para evaluar la cinética de fermentación, y después de 48 hdeincubacióndelasmuestrasseutilizaronparacuantificarla producción de AGV. La proteína cruda (%) (2.25 vs 2.73), EE (%) (0.274 vs 0.272), CF (%) (22.15 vs 16,58) y cenizas (%) (2.14 vs 1.81) no fueron diferentes estadísticamente (p> 0.05) entre AS y tratamiento AWC. Los valores de DNF (% MS) fueron estadísticamente diferentes (p< 0.05) entre elAS (45.45) y los tratamientosAWC (52.95). No se observaron diferencias enAGVentrebagazodeagaveydefrijol.Losvaloresdecalcio (%) promedio de 3.46 y 1.95 paraAS yAWC, respectivamente. Se concluye que, el bagazo de agave representa una alternativa viable para la alimentación de rumiantes, especialmente en la estación seca.

Effects of saline elicitors on saponin production in Agave salmiana plants grown in vitro

2021 ◽  
Vol 162 ◽  
pp. 476-482
Author(s):  
César A. Puente-Garza ◽  
Claudia A. Espinosa-Leal ◽  
Silverio García-Lara
Keyword(s):  
Agave Salmiana

In vitro assessment of agave fructans (Agave salmiana) as prebiotics and immune system activators

2014 ◽  
Vol 63 ◽  
pp. 181-187 ◽  
Author(s):  
L. Moreno-Vilet ◽  
M.H. Garcia-Hernandez ◽  
R.E. Delgado-Portales ◽  
N.E. Corral-Fernandez ◽  
N. Cortez-Espinosa ◽  
...  
Keyword(s):  
Immune System ◽  
Agave Salmiana ◽  
In Vitro Assessment

scholarly journals Prebiotic Effects of Agave salmiana Fructans in Lactobacillus acidophilus and Bifidobacterium lactis Cultures

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Adriana Castro-Zavala ◽  
Bertha I. Juárez-Flores ◽  
Juan M. Pinos-Rodríguez ◽  
Rosa E. Delgado-Portales ◽  
Juan R. Aguirre-Rivera ◽  
...  
Keyword(s):  
Optical Density ◽  
Dose Response ◽  
Lactobacillus Acidophilus ◽  
In Vitro Cultures ◽  
Bifidobacterium Lactis ◽  
Significant Difference ◽  
Agave Salmiana ◽  
Dose Response Effect ◽  
Density And Biomass

Agave salmiana is a fructan rich species that is widely distributed in Mexico. The aim of this investigation was to extract the fructans of A. salmiana and evaluate their prebiotic effect in 48 hours in vitro cultures of Bifidobacterium lactis and Lactobacillus acidophilus and to compare this effect with other available fructan sources. A significant difference in pH, optical density and biomass was found in the cultures depending on the source of fructans and the type of bacteria. It was possible to determine a dose-response effect of the A. salmiana fructans and the growth of the studied strains.

Effect of in vitro drought stress on phenolic acids, flavonols, saponins, and antioxidant activity in Agave salmiana

2017 ◽  
Vol 115 ◽  
pp. 400-407 ◽  
Author(s):  
César A. Puente-Garza ◽  
Cristina Meza-Miranda ◽  
Desiree Ochoa-Martínez ◽  
Silverio García-Lara
Keyword(s):  
Antioxidant Activity ◽  
Drought Stress ◽  
Phenolic Acids ◽  
Agave Salmiana

scholarly journals (277) Behavior of Glomus intraradices in Agave Roots Transformed with Agrobacterium rhizogenes

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1021C-1021
Author(s):  
Guillermo Rodríguez ◽  
Sergio Aguilar-Espinosa ◽  
Eugenio Perez-Molphe Balch ◽  
María del Rocío Flores-Bello ◽  
Javier Farias-Larios ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Petri Dish ◽  
Histochemical Staining ◽  
Root Induction ◽  
Transformed Roots ◽  
First Report ◽  
Agave Salmiana

The present work is the first report in vitro on root induction of Agave salmiana Otto, using Agrobacterium rhizogenes. Several concentrations of bacteria and acetosyringone were used, and different inoculation sites were tested, such as leaves, shaft, and root. Incubation time in darkness was 6 days. The transformed adventitious roots appeared 25 days after inoculation. The best treatment was when the shaft was inoculated with: 1 × 108 bacteria/mL and 100 μm acetosyringone; in this treatment, induction of transformed roots was 57.5% in the inoculated sites. The activity and presence of the foreign genes in the transformed roots of A. salmianawere verified as follows: 1) histochemical staining for GUS activity was determined in 80% of the tested root; and 2) molecular analysis via PCR was made to verify the presence of nptII gene and rol B gene (both were present in 60% of the tested root). This is the first report of the arbuscular mycorrhizal colonization on wild roots and transformed roots of Agavewith Glomus intraradicesSchenck and Smith. The result of the monoxenic culture was as follows: mother spore germinated 5 days; the colonization of the transformed roots was 70%. Then we proceeded to the recovery of daughter spores, in which we obtained an average 300 daughter spores per petri dish, 6 months after inoculation.

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

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