scholarly journals Enhancing adipose stem cell chondrogenesis: A study on the roles of dexamethasone, transforming growth factor β3 and ascorbate supplements and their combination

2017 ◽  
Vol 1 (1) ◽  
pp. 028-051
Author(s):  
Bernard J Van Wie
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Adipose Stem Cell

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

2012 ◽  
Vol 24 (8) ◽  
pp. 1098 ◽  
Author(s):  
Ruchi Sharma ◽  
Aman George ◽  
Nitin M. Kamble ◽  
Manmohan S. Chauhan ◽  
Suresh Singla ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Embryonic Stem ◽  
Transforming Growth Factor Β1 ◽  
Activin A ◽  
Feeder Layer ◽  
Es Cell ◽  
Fetal Fibroblasts ◽  
Tgf Β1

The present study examined the expression profile of buffalo fetal fibroblasts (BFF) used as a feeder layer for embryonic stem (ES) cell-like cells. The expression of important growth factors was detected in cells at different passages. Mitomycin-C inactivation increased relative expression levels of ACTIVIN-A, TGF-β1, BMP-4 and GREMLIN but not of fibroblast growth factor-2 (FGF-2). The expression level of ACTIVIN-A, transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-4 (BMP-4) and FGF-2 was similar in buffalo fetal fibroblast (BFF) cultured in stem cell medium (SCM), SCM + 1000 IU mL–1 leukemia inhibitory factor (LIF), SCM + 5 ng mL–1 FGF-2 or SCM + LIF + FGF-2 for 24 h whereas GREMLIN expression was higher in FGF-2-supplemented groups. In spent medium, the concentration of ACTIVIN-A was higher in FGF-2-supplemented groups whereas that of TGF-β1 was similar in SCM and LIF + FGF-2, which was higher than when either LIF or FGF-2 was used alone. Following culture of ES cell-like cells on a feeder layer for 24 h, the TGF-β1 concentration was higher with LIF+FGF-2 than with LIF or FGF-2 alone which, in turn, was higher than that in SCM. In the LIF + FGF-2 group, the concentration of TGF-β1 was lower and that of ACTIVIN-A was higher in spent medium at 24 h than at 48 h of culture. These results suggest that BFF produce signalling molecules that may help in self-renewal of buffalo ES cell-like cells.

scholarly journals Transforming Growth Factor-β and Notch Signaling Mediate Stem Cell Differentiation into Smooth Muscle Cells

Stem Cells ◽  
2010 ◽  
Vol 28 (4) ◽  
pp. 734-742 ◽  
Author(s):  
Kyle Kurpinski ◽  
Hayley Lam ◽  
Julia Chu ◽  
Aijun Wang ◽  
Ahra Kim ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Notch Signaling ◽  
Transforming Growth Factor ◽  
Stem Cell Differentiation ◽  
Muscle Cells ◽  
Transforming Growth Factor Β
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