scholarly journals ELISA r-p24: optimization and validation of an enzyme immunoassay for the diagnosis of Feline Immunodeficiency Virus infections

2017 ◽  
Vol 39 (2) ◽  
pp. 83-89
Author(s):  
Marli Sidoni ◽  
Hilton J. Nascimento ◽  
Mariana Miguez ◽  
José G. da Silva ◽  
Carlos Mazur ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Feline Immunodeficiency Virus ◽  
Virus Infections ◽  
Immunodeficiency Virus

scholarly journals Histologic Classification and Immunophenotype of Lymphosarcomas in Cats with Naturally and Experimentally Acquired Feline Immunodeficiency Virus Infections

1996 ◽  
Vol 33 (3) ◽  
pp. 264-272 ◽  
Author(s):  
J. J. Callanan ◽  
B. A. Jones ◽  
J. Irvine ◽  
B. J. Willett ◽  
I. A. P. McCandlish ◽  
...  
Keyword(s):  
B Cell ◽  
Feline Immunodeficiency Virus ◽  
Cell Activation ◽  
Polyclonal Antibodies ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
B Cell Activation ◽  
Virus Infections ◽  
Histologic Classification ◽  
Immunodeficiency Virus

Lymphosarcoma (malignant lymphoma) is the commonest hematopoietic tumor in the cat. Many cases are associated with feline leukemia virus (FeLV) infection, but epidemiologic and experimental data suggest that feline immunodeficiency virus (FIV) may also have a role in lymphomagenesis. In this paper, we describe the clinical presentation, histologic classification, and immunophenotype of lymphosarcoma in eight domestic cats with natural or experimental FIV infections. The tumors were often of unusual distribution, with the majority of cases conforming to the least common anatomic classification of “miscellaneous.” Histopathologic and immunophenotypic analysis using a panel of anti-cat and cross-reactive anti-human monoclonal and polyclonal antibodies identified seven of these tumors as high-grade B cell lymphomas of the centroblastic or immunoblastic subtypes. The remaining case was a T-cell tumor associated with a concurrent FeLV infection. Our findings, together with the results of an analysis of FIV proviral DNA in these tumors, indicate that the B-cell lymphosarcomas were comparable to those observed in human and simian immunodeficiency virus infections and that the role of FIV in lymphomagenesis is indirect and related to the potential for malignant transformation during polyclonal B cell activation.

Prevalences of feline leukaemia virus and feline immunodeficiency virus infections in cats in Sydney

1997 ◽  
Vol 75 (5) ◽  
pp. 323-327 ◽  
Author(s):  
R. MALIK ◽  
K. KENDALL ◽  
J. CRIDLAND ◽  
S. COULSTON ◽  
AJ STUART ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Virus Infections ◽  
Leukaemia Virus ◽  
Immunodeficiency Virus ◽  
Feline Leukaemia Virus

scholarly journals Feline immunodeficiency virus dendritic cell infection and transfer

2008 ◽  
Vol 89 (3) ◽  
pp. 709-715 ◽  
Author(s):  
Wendy S. Sprague ◽  
Melissa Robbiani ◽  
Paul R. Avery ◽  
Kevin P. O'Halloran ◽  
Edward A. Hoover
Keyword(s):  
T Cells ◽  
T Cell ◽  
Viral Replication ◽  
Feline Immunodeficiency Virus ◽  
De Novo ◽  
Cell Interactions ◽  
Virus Infections ◽  
Cell Infection ◽  
Immunodeficiency Virus ◽  
Viral Transfer

Feline immunodeficiency virus (FIV) interacts with dendritic cells (DC) during initiation of infection, but whether DC support or transfer FIV infection remains unclear. To address this issue, we studied the susceptibility of feline myeloid DC to FIV infection and assessed potential transfer of infection from DC to CD4+ T cells. FIV was detected in membrane-bound vesicles of DC within 2 h of inoculation, although only low concentrations of FIV DNA were found in virus-exposed isolated DC. Addition of resting CD4+ T cells increased viral DNA levels; however, addition of activated CD4+ T cells resulted in a burst of viral replication manifested by FIV p27 capsid antigen generation. To determine whether transfer of FIV infection required productively infected DC (vs virus bound to DC but not internalized), virus-exposed DC were cultured for 2 days to allow for degradation of uninternalized virus and initiation of infection in the DC, then CD4+ T blasts were added. Infection of T cells remained robust, indicating that T-cell infection is likely to be mediated by de novo viral infection of DC followed by viral transfer during normal DC/T-cell interactions. We conclude that feline DC support restricted FIV infection, which nevertheless is sufficient to efficiently transfer infection to susceptible T cells and trigger the major burst of viral replication. Feline DC/FIV/T-cell interactions (similar to those believed to occur in human immunodeficiency virus and simian immunodeficiency virus infections) highlight the means by which immunodeficiency-inducing lentiviruses exploit normal DC/T-cell interactions to transfer and amplify virus infection.

scholarly journals Epidemiology of Feline Foamy Virus and Feline Immunodeficiency Virus Infections in Domestic and Feral Cats: a Seroepidemiological Study

1999 ◽  
Vol 37 (9) ◽  
pp. 2848-2851 ◽  
Author(s):  
I. G. Winkler ◽  
M. Löchelt ◽  
R. L. P. Flower
Keyword(s):  
Aggressive Behavior ◽  
Feline Immunodeficiency Virus ◽  
Foamy Virus ◽  
Social Contact ◽  
Virus Infections ◽  
Domestic Cats ◽  
Feral Cats ◽  
Immunodeficiency Virus ◽  
Foamy Viruses ◽  
Wild Cats

Although foamy viruses (Spumaviruses) have repeatedly been isolated from both healthy and diseased cats, cattle, and primates, the primary mode of transmission of those common viruses remains undefined. A database of the feline foamy virus (FeFV) and feline immunodeficiency virus (FIV) antibody status, age, and sex of 389 domestic cats presented to veterinarians was assembled. A similar database for 66 feral (wild) cats was also assembled. That FeFV antibody status reflects infection was validated by PCR. Both FeFV and FIV infection rates were found to gradually increase with age, and over 70% of cats older than 9 years were seropositive for FeFV. In domestic cats, the prevalence of FeFV infection was similar in both sexes. In feral cats, FeFV infection was more prevalent in female cats than in male cats. Although both FeFV and FIV have been reported to be transmitted by biting, the patterns of infection observed are more consistent with an interpretation that transmission of these two retroviruses is not the same. The prevalence of FIV infection is highest in nondesexed male cats, the animals most likely to display aggressive behavior. The gradual increase in the proportion of FeFV-infected animals is consistent with transmission of foamy viruses by intimate social contact between animals and less commonly by aggressive behavior.

Sign in / Sign up

Export Citation Format

Share Document