scholarly journals Molecular detection and hematological changes in dogs naturally infected by Babesia vogeli in metropolitan region of Rio de Janeiro

2020 ◽  
Vol 42 ◽  
Author(s):  
Renata Quintela Assad ◽  
Eloy da Silva Seabra ◽  
Monique Moraes Lambert ◽  
Carmen Beatriz Seti Corrêa ◽  
Tatiana Didonet Lemos ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Molecular Detection ◽  
Metropolitan Region ◽  
Babesia Vogeli ◽  
Hematological Changes

scholarly journals Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niterói, Rio de Janeiro

2020 ◽  
Vol 29 (3) ◽  
Author(s):  
Fernanda Barbosa dos Santos ◽  
Gilberto Salles Gazeta ◽  
Laís Lisboa Correa ◽  
Lucas Fernandes Lobão ◽  
João Pedro Palmer ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Clinical Laboratory ◽  
18S Rrna Gene ◽  
Metropolitan Region ◽  
Rrna Gene ◽  
Babesia Vogeli ◽  
Hematological Changes ◽  
Pcr Targeting ◽  
Canine Piroplasmosis

Abstract Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.4%) were positive and show adequate nucleotide sequences for interpretation with identity between 93%-100% with Babesia vogeli in compared to the sequences isolated of infected dogs from other states in Brazil deposited on GenBank. Most of dogs infected with B. vogeli had anemia (62.5%) and thrombocytopenia (95.8%). The findings of this study are compatible with previous reports in the literature and highlight B. vogeli as the most incriminated species in canine piroplasmosis in Brazil, and thrombocytopenia the hematological alteration most frequently identified in this infection. It is important to note that this is the first study involving the molecular characterization of piroplasms in the metropolitan region of Rio de Janeiro, based on PCR followed by sequencing.

scholarly journals Ehrlichia spp. infection in domestic cats from Rio de Janeiro State, southeast Brazil

2019 ◽  
Vol 28 (1) ◽  
pp. 180-185 ◽  
Author(s):  
Andresa Guimarães ◽  
Juliana Macedo Raimundo ◽  
Raisa Braul Rodrigues ◽  
Maristela Peckle Peixoto ◽  
Huarrisson Azevedo Santos ◽  
...  
Keyword(s):  
16S Rrna ◽  
Rio De Janeiro ◽  
Molecular Detection ◽  
Rrna Gene ◽  
Igg Antibodies ◽  
Domestic Cats ◽  
Rickettsial Infection ◽  
Hematological Changes ◽  
Rrna Sequences ◽  
Janeiro State

Abstract Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p<0.05). This study showed that Ehrlichia spp. occur in domestic cats from Greater Rio de Janeiro. Further studies involving culture isolation are much needed to more precisely characterize these organisms.

scholarly journals Bartonella spp. and hematological changes in privately owned domestic cats from Rio de Janeiro, Brazil

2017 ◽  
Vol 11 (08) ◽  
pp. 591-596 ◽  
Author(s):  
Aline Moreira Souza ◽  
Nadia Regina Pereira Almosny ◽  
Alexsandra Rodrigues Mendonça Favacho ◽  
Daniele Nunes Pereira Almeida ◽  
Renata Fernandes Ferreira ◽  
...  
Keyword(s):  
Metropolitan Area ◽  
Rio De Janeiro ◽  
Citrate Synthase ◽  
Metropolitan Region ◽  
Domestic Cats ◽  
Normal Limits ◽  
Complete Blood Counts ◽  
Hematological Changes ◽  
Privately Owned ◽  
Janeiro State

Introduction: Bartonella infection in cats can represent a risk to owners, particularly today when considering the increase in cat populations and their role in human bartonellosis epidemiology. In the present study, we aimed to detect Bartonella spp. in blood samples from 163 asymptomatic privately-owned cats from the metropolitan area of Rio de Janeiro State, Brazil by using a conventional PCR test and also to evaluate the association between Bartonella spp. and hematological changes in positive cats. Methodology: PCR assays were performed targeting the Bartonella spp heat shock protein (htrA) gene and complete blood counts were also performed in all samples. Positive PCR samples were confirmed by the presence of two genes, citrate synthase (gltA) and RNA polymerase beta-subunit-encoding (rpoB). Results: A total of 74.85% (122/163) of the tested cats were positive for Bartonella spp and partial sequencing confirmed to be B. henselae. All hematological findings from the 163 cats tested (PCR-positive and negative), presented normal limits. Conclusions: This study demonstrates that B. henselae is present in almost 75% asymptomatic privately-owned domestic cats in the metropolitan region of Rio de Janeiro State, Brazil. Our results also show that hematological findings in Bartonella spp. infected cats are uncommon. In this scenario, the use of PCR as a diagnostic tool in feline Bartonella infections should be considered. Finally, these results also demonstrate the potential risk of Bartonella spp. infection in the human population of the metropolitan area of Rio de Janeiro State, Brazil.

scholarly journals Detection of Chikungunya Virus in Saliva and Urine Samples of Patients from Rio de Janeiro, Brazil. A Minimally Invasive Tool for Surveillance

2021 ◽  
Author(s):  
Tiago Souza Salles ◽  
Thayane Encarnação Sá-Guimarães ◽  
Victor Guimarães-Ribeiro ◽  
Ana Claudia Amaral Melo ◽  
Davis Fernandes Ferreira ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Rio De Janeiro ◽  
Chikungunya Virus ◽  
Virus Genome ◽  
Urine Samples ◽  
Metropolitan Region

Abstract In this study, we collected saliva and urine samples from individuals in the metropolitan region of Rio de Janeiro, Brazil, during the years of 2017 through 2019 and we were able to detect the presence of Chikungunya virus genome in these samples. Our findings reinforce the possibility to monitor Chikungunya virus circulation by analyzing saliva and urine from individuals during inter-epidemic periods.

Geodesign Applied to Propositional Scenarios of Medium and Long-Term Sustainable Projects for Rio de Janeiro Metropolitan Region, Brazil

2021 ◽  
pp. 437-447
Author(s):  
Tiago Badre Marino ◽  
Cézar Augusto Barra Rocha ◽  
Ashiley Adelaide Rosa ◽  
Tiago Augusto Gonçalves Mello
Keyword(s):  
Rio De Janeiro ◽  
Metropolitan Region ◽  
Sustainable Projects

Accountants' IT responsibilities and competencies from a student perspective

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Raphael Junger da Silva ◽  
Roberto Tommasetti ◽  
Monica Zaidan Gomes ◽  
Marcelo Alvaro da Silva Macedo
Keyword(s):  
Rio De Janeiro ◽  
Research Question ◽  
Metropolitan Region ◽  
Accounting Students ◽  
Student Perspective ◽  
Content Type ◽  
Strategic Role ◽  
Work Integrated Learning ◽  
Strongly Agree ◽  
Interdisciplinary Courses

PurposeResearch question is about the perception of stricto sensu undergraduate and graduate accounting students, from the Metropolitan Region of Rio de Janeiro (Brazil) of the accountants' responsibilities and competencies required to them in assisting IT/IS strategy definition and implementation.Design/methodology/approachThis is a descriptive research, using a qualitative paper-and-pencil approach. Designed based on United Nations Accounting Model Curriculum, a questionnaire was self-applied to 361 accounting students of eight higher education institutions (HEIs) in Rio de Janeiro, whose responses are quantitatively analysed.FindingsAccounting students strongly agree on the need to acquire strategic skills that enable them to assist organizations in IT/IS issues but are quite divided about the related accountants' responsibilities, underestimating their future role in this respect.Research limitations/implicationsAlthough quantitatively adequate, the sample is non-probabilistic and, thus, results cannot be generalized. The authors expect that this paper can encourage HEIs to engage the path of multidisciplinary education and rethink their silo-based pedagogical models which have to prepare students for their forthcoming professional challenges, by introducing interdisciplinary courses, soft skills disciplines and work-integrated learning activities.Practical implicationsFindings can be useful for teachers and coordinators of the IT/IS area in accounting courses, stimulating brainstorming on the accountant's role in assisting organizations in IT/IS strategies and practices implementation. The study has relevant implications for educators through the identification and analysis of difficulties faced by students.Originality/valueThis study contributes to the research base of accountants' strategic role since the responsibility awareness identified gap can be elaborated for stimulating the discussion about the centrality of the accountant in IT processes within organizations.

scholarly journals Molecular Analysis of Canine Filaria and Its Wolbachia Endosymbionts in Domestic Dogs Collected from Two Animal University Hospitals in Bangkok Metropolitan Region, Thailand

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 114 ◽  
Author(s):  
Hathaithip Satjawongvanit ◽  
Atchara Phumee ◽  
Sonthaya Tiawsirisup ◽  
Sivapong Sungpradit ◽  
Narisa Brownell ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Neglected Tropical Diseases ◽  
Control Strategies ◽  
Domestic Dogs ◽  
Metropolitan Region ◽  
World Health ◽  
University Hospitals ◽  
Effective Prevention ◽  
Blood Samples ◽  
Filarial Nematodes

Canine filariasis is caused by several nematode species, such as Dirofilaria immitis, Dirofilaria repens, Brugia pahangi, Brugia malayi, and Acanthocheilonema reconditum. Zoonotic filariasis is one of the world’s neglected tropical diseases. Since 2000, the World Health Organization (WHO) has promoted a global filarial eradication program to eliminate filariasis by 2020. Apart from vector control strategies, the infection control of reservoir hosts is necessary for more effective filariasis control. In addition, many studies have reported that Wolbachia is necessary for the development, reproduction, and survival of the filarial nematode. Consequently, the use of antibiotics to kill Wolbachia in nematodes has now become an alternative strategy to control filariasis. Previously, a case of subconjunctival dirofilariasis caused by Dirofilaria spp. has been reported in a woman who resides in the center of Bangkok, Thailand. Therefore, our study aimed to principally demonstrate the presence of filarial nematodes and Wolbachia bacteria in blood collected from domestic dogs from the Bangkok Metropolitan Region, Thailand. A total of 57 blood samples from dogs with suspected dirofilariasis who had visited veterinary clinics in Bangkok were collected. The investigations for the presence of microfilaria were carried out by using both microscopic and molecular examinations. PCR was used as the molecular detection method for the filarial nematodes based on the COI and ITS1 regions. The demonstration of Wolbachia was performed using PCR to amplify the FtsZ gene. All positive samples by PCR were then cloned and sequenced. The results showed that the filarial nematodes were detected in 16 samples (28.07%) using microscopic examinations. The molecular detection of filarial species using COI-PCR revealed that 50 samples (87.72%) were positive; these consisted of 33 (57.89%), 13 (22.81%), and 4 (7.02%) samples for D. immitis, B. pahangi, and B. malayi, respectively. While the ITS1-PCR showed that 41 samples (71.93%) were positive—30 samples (52.63%) were identified as containing D. immitis and 11 samples (19.30%) were identified to have B. pahangi, whereas B. malayi was not detected. Forty-seven samples (82.45%) were positive for Wolbachia DNA and the phylogenetic tree of all positive Wolbachia was classified into the supergroup C clade. This study has established fundamental data on filariasis associated with Wolbachia infection in domestic dogs in the Bangkok Metropolitan Region. An extensive survey of dog blood samples would provide valuable epidemiologic data on potential zoonotic filariasis in Thailand. In addition, this information could be used for the future development of more effective prevention and control strategies for canine filariasis in Thailand.

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