scholarly journals Associations of clinical features of functional bowel disorders with gut microbiota characteristics in adolescents: A pilot study

2021 ◽  
Vol 6 (6-2) ◽  
pp. 73-81
Author(s):  
A. I. Romanitsa ◽  
U. M. Nemchenko ◽  
A. V. Pogodina ◽  
E. V. Grigorova ◽  
N. L. Belkova ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Clinical Manifestations ◽  
Enzymatic Properties ◽  
Functional Bowel Disorders ◽  
Obese Adolescents ◽  
E Coli ◽  
Bowel Movements ◽  
Bowel Disorders ◽  
The Relationship ◽  
Clostridium Spp

Background. Obesity is a multifactorial disease that is one of the important public health problems in the 21st century. Functional bowel disorders in obese children and adolescents are one of the most common pathologies of the digestive tract. The aim: to characterize the bacteriological composition of the colon microbiota in obese adolescents and identify its associations with the clinical manifestations of functional bowel disorders.Materials and methods. The study included 20 adolescents aged 11–17 years. Stool consistency was assessed using the Bristol Stool Scale. The study of biological material was carried out using standard bacteriological methods. To assess the strength of the relationship between the nominal variables, Cramer’s V test was used.Results. It has been shown that in adolescents with obesity and functional bowel disorders, there is a decrease in the number of representatives of bifidoflora, an increase in the microbial density of Escherichia coli with altered enzymatic properties and an excessive growth of potentially pathogenic enterobacteria. In obese adolescents with functional bowel disorders, abdominal pain associated with the act of defecation is associated with the presence of enterobacteria and E. coli with altered enzymatic properties. Rare bowel movements are associated with an increased abundance of Enterococcus spp. and the presence of opportunistic Enterobacteriaceae microorganisms. Frequent bowel movements are associated with the presence of E. coli with altered enzymatic properties and Clostridium spp. In the presence of loose stool, Clostridium spp. is present; unstable stool (alternation of hard and liquid stools in one patient) is associated with a decrease in the number of lactobacilli and E. coli with normal enzymatic properties, the appearance of E. coli with altered enzymatic properties, opportunistic microorganisms of Enterobacteriaceae family and Clostridium spp. Conclusion. The relationship between the clinical symptoms of functional bowel disorders and changes in the composition of the gut microbiota in obese adolescents may be a justification for conducting in-depth studies aimed at finding early predictors of functional bowel diseases associated with obesity in the adult cohort.

scholarly journals Probiotics and Colon Cancer

2019 ◽  
Vol 7 (3) ◽  
pp. 66 ◽  
Author(s):  
Lorenzo Drago
Keyword(s):  
Colon Cancer ◽  
Radiation Therapy ◽  
Gastrointestinal Tract ◽  
Gut Microbiota ◽  
Immune Modulation ◽  
Average Frequency ◽  
Scientific Interest ◽  
Positive Action ◽  
Bowel Movements ◽  
The Relationship

Literature has recently highlighted the enormous scientific interest on the relationship between the gut microbiota and colon cancer, and how the use of some selected probiotics can have a future impact on the adverse events which occur during this disease. Although there is no clear evidence to claim that probiotics are effective in people with cancer, recent reviews have found that probiotics can significantly reduce the incidence of diarrhea and the average frequency of daily bowel movements. However, most of this evidence needs to be more clinically convincing and further discussed. Undoubtedly, some probiotics, when properly dosed and administered, can have a strong rebalance effect on the gut microbiota and as a consequence a possible positive action on immune modulation of the gastrointestinal tract and on inflammation of the intestinal mucosa. Many recent findings indeed support the hypothesis that the daily use of some selected probiotics can be a feasible approach to effectively protect patients against the risk of some severe consequences due to radiation therapy or chemotherapy. This paper aims to review the most recent articles in order to consider a possible adjuvant approach for the use of certain well-balanced probiotics to help prevent colon cancer and the adverse effects caused by related therapies.

scholarly journals Effect of Combined Live Probiotics Alleviating the Gastrointestinal Symptoms of Functional Bowel Disorders

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Jin Shi ◽  
Feng Gao ◽  
Jie Zhang
Keyword(s):  
Gut Microbiota ◽  
Bowel Preparation ◽  
Gastrointestinal Symptoms ◽  
Functional Bowel Disorders ◽  
Control Groups ◽  
Probiotic Supplementation ◽  
Positive Rate ◽  
Bowel Disorders ◽  
Relative Abundances ◽  
And Control

Objective. Changes of the gut microbiota are related to the pathogenesis of functional bowel disorders (FBDs), and probiotic supplementation may be an effective treatment option. Therefore, we aimed to investigate the effect of combined live probiotics on the gastrointestinal symptoms of FBDs via altering the gut microbiota. Methods. Patients with the gastrointestinal symptoms of FBDs attending the Outpatient Department, from July to November 2019, were recruited. After the bowel preparation with polyethylene glycol electrolyte powder and colonoscopy, patients with normal result of colonoscopy were randomly divided into the probiotics group and control group. Patients in the probiotics group were prescribed with combined live Bacillus subtilis and Enterococcus faecium enteric-coated capsules for 4 weeks. Small intestinal bacteria overgrowth (SIBO) was measured by lactulose hydrogen breath test, and the microbial DNA was extracted from the fecal samples and the bacteria were classified by 16S rDNA gene amplicon sequencing. Results. Twenty-five patients of each group were recruited, and there was no significant difference between the probiotics and control groups on baseline gastrointestinal symptom rating scale (GSRS), positive rate of SIBO, and relative abundances of the gut microbiota at the phylum level. After 4 weeks of treatment, the values of the probiotics and control groups were as follows: GSRS 1.4±1.4 and 3.6±1.6 and positive rate of SIBO 28.0% and 56.0%, respectively. The median relative abundances of the gut microbiota were 1.01% and 5.03% Actinobacteria and 43.80% and 35.17% Bacteroidetes at the phylum level; 0.76% and 3.29% Bifidobacterium, 0.13% and 0.89% Cillinsella, 0.03% and 0.01% Enterococcus, 0.18% and 0.36% Lachnospiraceae, 0.10% and 0.16% Ruminococcus torques group, 1.31% and 2.44% Blautia, and 0.83% and 2.02% Fusicatenibacter at the genus level (P<0.05), respectively. Conclusion. Combined live probiotic supplementation after the bowel preparation can alter the gut microbiota, decontaminate SIBO, and alleviate the gastrointestinal symptoms of FBDs. This trial is registered with ChiCTR1900026472.

011 Shiftwork, Functional Bowel Symptoms and the Microbiome

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A6-A6
Author(s):  
Ann Rogers ◽  
Yijuan Hu ◽  
Ye Yue ◽  
Emily Wessel ◽  
Jennifer Christie ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Beta Diversity ◽  
Time Of Day ◽  
Full Time ◽  
Functional Bowel Disorders ◽  
Shift Workers ◽  
Intestinal Microbes ◽  
Two Samples ◽  
Bowel Disorders ◽  
Night Shifts

Abstract Introduction There are about 15 million Americans working full-time on evening, night, or rotating shifts. Between 48% and 81.9% of those working rotating or night shifts report abdominal pain, constipation, diarrhea and other symptoms of functional bowel disorders. The basis for this high prevalence of functional bowel disorders, including irritable bowel syndrome (IBS), among shift workers is unknown. Animal studies, however, suggest that circadian disruption, similar to that in shift workers, may contribute to the development of GI complaints among shift workers by altering the composition and normal diurnal rhythmicity of the resident intestinal microbes. Methods Fifty-one full time staff nurses who worked either 12-hour day or night shifts completed demographic information, the Rome III IBS module, daily symptoms diaries for 14 days. They also collected two samples of gut microbiota before the beginning and at the end of their last work shift on day 14, using validated field-tested methods consistent with the Human Microbiome Project. After DNA extraction, 16S rRNA sequencing and assignment to the genus level was completed, samples were then compared to determine if there were 1) differences in the diversity and profile of the microbiome by shift type; 2) if there were differences in the microbiome by time of day for collection; and 3) whether there were differences in the diversity and profile of the microbiome of nurses with IBS and those without IBS. Results There were no differences in alpha or beta diversity of gut microbiota when specimens from day and night shift nurses were compared. There were however marginal differences in beta diversity when specimens collected at the beginning and end of the shifts were compared, with seven OTUs being differentially abundant when collected from day shift workers in the evening. There were also three OTUs to be differentially abundant in participants reporting IBS symptoms. Conclusion These data did not reveal strong effects of circadian alterations in gut microbiota related to shiftwork. Other factors, such as insufficient sleep and dietary intake during the data-gathering period, need to be explored Support (if any) Emory University Synergy Grant Program

scholarly journals Shiftwork, functional bowel symptoms, and the microbiome

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11406
Author(s):  
Ann E. Rogers ◽  
Yi-Juan Hu ◽  
Ye Yue ◽  
Emily F. Wissel ◽  
Robert A. Petit III ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Beta Diversity ◽  
Night Shift ◽  
Time Of Day ◽  
Full Time ◽  
Functional Bowel Disorders ◽  
Shift Workers ◽  
Bowel Symptoms ◽  
Bowel Disorders ◽  
Night Shifts

Background There are about 15 million Americans working full-time on evening, night, or rotating shifts. Between 48% and 81.9% of those working rotating or night shifts report abdominal pain, constipation, diarrhea and other symptoms of functional bowel disorders. The basis for this high prevalence of functional bowel disorders, including irritable bowel syndrome (IBS), among shift workers is unknown. Animal studies, however, suggest that circadian disruption, similar to that in shift workers, may contribute to the development of GI complaints among shift workers by altering the composition and normal diurnal rhythmicity of the resident intestinal microbes. Therefore, the present study was designed to determine if there were differences in (1) composition and diversity of the microbiome of night shift workers compared to day shift workers; and (2) the composition and diversity of the microbiome among shift workers experiencing functional bowel symptoms compared to shift workers who did not experience functional bowel symptoms. Methods Fifty-one full time staff nurses who worked either 12-hour day or night shifts completed demographic information, and the Rome III IBS module. They also collected two samples of gut microbiota before the beginning and at the end of their last work shift on day 14, using validated field-tested methods consistent with the Human Microbiome Project. After DNA extraction, 16S rRNA sequencing and assignment to the genus level was completed, samples were then compared to determine if there were (1) differences in the diversity and profile of the microbiome by shift type; (2) if there were differences in the microbiome by time of day for collection; and (3) whether there were differences in the diversity and profile of the microbiome of nurses with IBS and those without IBS. Results There were no differences in alpha or beta diversity of gut microbiota when specimens from day and night shift nurses were compared. There were however marginal differences in beta diversity when specimens collected at the beginning and end of the shifts were compared, with seven OTUs being differentially abundant when collected from day shift workers in the evening. There were also three OTUs to be differentially abundant in participants reporting IBS symptoms.

scholarly journals Obesity and functional bowel disorders in children

2021 ◽  
Vol 12 (1) ◽  
pp. 59-69
Author(s):  
Anna V. Pogodina ◽  
Anastasia I. Romanitsa ◽  
Lyubov V. Rychkova
Keyword(s):  
Psychological Factors ◽  
Eating Habits ◽  
Low Grade ◽  
Functional Bowel Disorders ◽  
Associated Conditions ◽  
Bowel Disorders ◽  
Common Risk Factors ◽  
Irritable Bowel ◽  
Low Grade Inflammation ◽  
The Relationship

Obesity and functional bowel disorders (FBDs) are often observed in children and share common risk factors. However, the possible relationship between these conditions has begun to be studied recently and the results obtained are ambiguous. Therefore, it is important to summarize the literature on the relationship between obesity and FBDs in children and to discuss the possible pathophysiological mechanisms mediating this. The literature suggests that obesity and FBDs are likely associated conditions. A significant link between obesity and irritable bowel syndrome in children has been confirmed by all studies on this problem. There is also a many evidence that obesity in the pediatric cohort is associated with constipation. However, it should also be noted that there are few studies of this issue, they are heterogeneous in the composition of participants and the diagnostic criteria used, in most cases they are unadjusted for potential confounders. The link between obesity and FBDs may be mediated by diet, eating habits, and psychological factors. But the most promising direction in studying the association between these conditions may be studies of the gut microbiota, changes in which can contribute to impaired intestinal immune function, the development of chronic low-grade inflammation, increased intestinal permeability, impaired motility and visceral hypersensitivity. To date, there is insufficient data to confidently confirm the existence and nature of the relationship between obesity and FBDs in children, but research in this direction can provide important information for the development of approaches to treatment, prevention and early diagnosis of both groups of diseases.

scholarly journals Dysbiosis in Functional Bowel Disorders

2018 ◽  
Vol 72 (4) ◽  
pp. 296-306 ◽  
Author(s):  
Paul Enck ◽  
Nazar Mazurak
Keyword(s):  
Gut Microbiota ◽  
Fecal Microbiota Transplantation ◽  
Healthy Person ◽  
Fecal Microbiota ◽  
Functional Bowel Disorders ◽  
Microbial Composition ◽  
Bowel Disorders ◽  
Irritable Bowel ◽  
Benign Disorders

Functional bowel disorders (FBD) resemble a group of diseases of the gastrointestinal (GI) tract that are without a clear pathogenesis; the best known is probably the “irritable bowel syndrome” (IBS). Only recently we have been able to explore the role of the gut microbiota in FBD due to progress in microbiological analytic techniques. There are different ways to explore the role of the gut microbiota and its dysbiosis in FBD. Comparison of the microbial composition in a group of patients with FBD, for example, with IBS to a group of healthy volunteers is one way. Studies have shown that the microbiota in FBD is different from that of healthy controls, but the recorded differences are not necessarily specific for FBD, they may also occur in other diseases. Another approach to explore the role of the gut microbiota in FBD is to challenge the existing “flora” with novel bacteria (probiotics) or with nutritional substrates that stimulate bacterial growth (prebiotics). More than 60 such trials including several thousand patients have been performed in IBS. These studies have produced mixed outcome: some probiotics appear to be better than others, and some appear to work only for a part of the IBS symptoms and not for all. An extreme form of this approach is the transfer of an entire microbiota from 1 healthy person to another, called fecal microbiota transplantation. This has rarely been tested in FBD but is not without risk in benign disorders.

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