scholarly journals Determination of Total Phenolic, Flavonoid Content and Antioxidant Activity of Campolay (Pouteria campechiana (Kunth) Baehni) Extract

Author(s):  
Nurul Hidayah ◽  
Sani Nurlaela Fitriansyah ◽  
Diah Lia Aulifa ◽  
Siska Dewi ◽  
Windania Barkah
2020 ◽  
Vol 19 (1) ◽  
pp. 61-70
Author(s):  
Erdal Ağlar ◽  
Ahmet Sümbül ◽  
Orhan Karakaya ◽  
Burhan Ozturk

The study was conducted in 2017 in the district of Sivas. In the study, 20 genotypes, which are considered to be different from each other, taking into account the fruit characteristics such as color and size and shape, were determined from the hawthorns that were naturally grown in the flora of Suşheri. At harvest time, the fruit, which would be adequate for pomological and biochemical measurements and analyzes, was harvested. According to the results of the measurements and analyzes in the study, fruit weight was found to vary between 0.68 g and 6.35 g, fruit width was between 10.52 and 29.48 mm and fruit length was between 11.40 and 20.67 mm. The highest firmness values were recorded with the genotype (G) 20 genotype, while the G4 had the lowest values in terms of the firmness values of the fruit flesh. While there are no significant differences between the pH values of the genotypes, the differences between the genotypes in terms of SSC, TA and vitamin C contents are quite significant. It has been found that there are significant differences between the genotypes in terms of total phenolic, total flavonoid and antioxidant activity. The total phenolic content ranged from 218.8 (G17) to 605.8 (G5 and G8) mg GAE kg–1 f.w., while the lowest total flavonoid content was 21.58 (G 17) and the highest total flavonoid content was 67.75 (G9) mg GAE kg–1 f.w. When the antioxidant activity was evaluated, the DPPH values were 1.08 (G17) – 15.43 (14) mmol TE kg–1 f.w., the FRAP values were 15.43 (G16) – 47.23 (G8) mmol TE kg–1 f.w. respectively.


2021 ◽  
Vol 15 (1) ◽  
Author(s):  
João Vitor Souza Ramada ◽  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinicius Abadia Ventura ◽  
Hellen Regina Fernandes Batista-Ventura ◽  
Celiana Maria Ferrarini Triches ◽  
...  

Himatanthus obovatus is a tree species that has laticifers in its aerial organs, where it is used in folk medicine. The aim of this work was to evaluate the latex of H. obovatus regarding its phytochemical composition and biological activities. The latex was collected and lyophilized, yield (%) was carried out, phytochemical prospection for several groups, determination of the content of flavonoids, phenolics and total tannins, antioxidant activity in reducing DPPH, antifungal activity on the Candida genus, antibacterial for Escherichia coli, Staphylococcus aureus, Salmonella serovar Enteritidis and Thyphymurium and Enterococcus faecalis, hemolytic activity on human erythrocytes and cytotoxicity bioassay on Artemia salina. The latex yield was 19.05%, positive presence of several groups of phytocompounds was observed, total flavonoid content of 78.80 mg QE 100 g-1, total phenolic compounds of 166.51 mg AGE g 100-1, total tannins of 14.81 mg TAE 100 g-1, antioxidant activity IC50 = 178.87 µg mL-1, antifungal activity for all Candida strains and antibacterial only for E. coli and E. faecalis, hemolytic activity between 68-18% and cytotoxicity with LC50 = 433.97 µg mL-1 on A. salina. The latex of Himatanthus obovatus demonstrated in this study, important phytochemical data and biological activities.


2015 ◽  
Vol 9 (5) ◽  
pp. 122-125
Author(s):  
Nahid Rastakhiz ◽  
Fereshteh Khosravi ◽  
Seyyed Sina Seyyed Jafari Olia ◽  
Behzad Iranmanesh

This study describes the determination of some phenolic compounds in four different vegetables of Kerman in Iran including Carrots, Celery, Lettuce and red cabbage .The  phenolic compounds analyzed were (Ascorbate, Ferulic acid, Naringin) using reverse phase high performance liquid chromatography (RPUPLC). The results of analysis showed in vegetables ranged between 90 and 1080mg /kg. A high and significant correlation between antioxidant activity and total phenolic content was determined in vegetables (r2 = 0.9461 P < 0.06). However, flavonoid content was not significantly correlated with antioxidant activity in vegetables. It was observed that total phenolic content is the major contributor to the antioxidant activity of vegetables.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12712


2021 ◽  
Vol 11 (11) ◽  
pp. 4745
Author(s):  
Toncho Dinev ◽  
Milena Tzanova ◽  
Katya Velichkova ◽  
Diyana Dermendzhieva ◽  
Georgi Beev

Plant extracts are an important alternative to antibiotics, which are ever more restricted because of their developing microbial resistance and some adverse effects that have been observed following frequent application. The aim of the present study was to determine the antifungal and antioxidant activity of the methanolic extracts of Acorus calamus, Chlorella vulgaris, Lemna minuta and Scenedesmus dimorphus. The antifungal activity of the extracts against strains of Aspergillus flavus, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus niger, Aspergillus carbonarius, Fusarium graminearum, Fusarium oxysporum, Penicillium chrysogenum and Alternaria alternata was evaluated via the agar well diffusion method. The antioxidant activity of the extracts was measured through the determination of three parameters—total phenolic content, total flavonoid content and radical scavenging potential (determined through UV/Vis analysis). A. calamus extracts had the highest antimicrobial activity against eight fungal strains, followed by the C. vulgaris, L. minuta and S. dimorphus extracts, which were inhibitory against two to three strains. Among the extracts from the species studied, the extract from S. dimorphus showed the highest antioxidant potential, as determined via the DPPH (1,1’-diphenyl-2-picrylhydrazil-radical) method. This correlated to its high total phenolic and flavonoid content. From A. calamus and L. minuta, methanolic extracts were obtained that exhibited similar values of the aforementioned parameters, followed by C. vulgaris extracts, which showed the lowest antioxidant activity. Based on the Pearson correlation coefficients, the impacts of the total phenolic content and the total flavonoid content on radical scavenging capacity are similar, and flavonoids were a significant part of the total phenolic compounds extracted from the plant materials studied.


2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Aslı Özkök ◽  
Merve Keskin ◽  
Aslı Elif Tanuğur Samancı ◽  
Elif Yorulmaz Önder ◽  
Çiğdem Takma

AbstractThis study aimed to determine the standard amount of antioxidant content and compounds of the propolis for the standardization of propolis. For this purpose, the total flavonoids, total phenolic, CUPRAC antioxidant capacity content and the diversity of phenolic and flavonoid components of these propolis samples were found by HPLC determined at the 23 propolis samples which were collected different regions of Turkey. Beside that, the similarities and differences of these 23 provinces to each other according to their antioxidant capacities were investigated by multidimensional scaling analysis. The total flavonoid content in the propolis samples were determined between 21.28 and 152.56 mg CE/g. The total phenolic content in the propolis samples was found between 34.53 mg and 259.4 mg GAE/g. CUPRAC antioxidant capacity of the propolis samples and antioxidant range was found from 95.35 to 710.43 mg TE/g. Also, 4 flavonoid [Quercetin (min.1.12–max.4.14 mg/g), Galangin (min.0.72–max.40.79 mg/g), Apigenin (min.1.07–max.17.35 mg/g), Pinocembrin (min.1.32–max.39.92 mg/g] and 6 phenolic acid [Caffeic acid (min.1.20–max.7.6 mg/g), p-Coumaric acid (min.1.26–max.4.47 mg/g), trans-Ferulic acid (min.1.28–max.4.92 mg/g), Protocatechuic acid (1.78 mg/g), trans-Cinnamic acid (min.1.05–max.3.83 mg/g), Caffeic Acid Phenethyl Ester (CAPE) (min.1.41–max.30.15 mg/g)] components were detected as mg/g, in different ratios in propolis samples collected from different regions. The feature of this study, so far, is to have the maximum number of samples representing the Turkish propolis, and so is thought to help to national and international propolis standard workings.


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