scholarly journals Evaluation of the physico-chemical state of the soil contaminant isolate indigenous bacterial species

2021 ◽  
Vol 17 (2) ◽  
pp. 139-151
Author(s):  
Nwankwo CC ◽  
John Godson N ◽  
Daodu Bamidele T

The aim of the study is to isolate indigenous bacterial species which have the ability to degrade crude oil. Samples were collected from an oil polluted site in Ejama-Ebubu Eleme Rivers state. The samples were taken from five spot (A-E), of depth 0-15 (A1– E1) and 15-30 (A2– E2) in sterile polyethylene bags, using appropriate equipment, then taken to the laboratory for analysis, Physicochemical parameters such as pH, nitrate, sulphate, phosphate, total petroleum hydrocarbon (TPH), poly aromatic hydrocarbon (PAH), salinity, temperature, conductivity and heavy metals (iron, zinc, nikel, lead, chromium) were determined, The THB count was determined using the spread plate method on nutrient agar. Soil, sediment and water physicochemical parameters determined indicated that the samples had been exposed to hydrocarbon contamination. The Gram negative bacteria belonging to the genus pseudomonas is the most frequent. Other genera isolated were Nocardia, Micrococcus,Chromobacterium, Burkholdia, Corynebacterium. The study revealed the presence of petroleum hydrocarbons in the Ejama_Ebubu site as well as known genera of hydrocarbon utilizing bacteria. The biases associated with culture-dependent microbial enumeration techniques may limit the full description of the bacterial diversity in Ejama-Ebubu site. From the study, it is concluded that microorganisms that can degrade hydrocarbons are found in oil contaminated soil and can easily be isolated from these contaminated sites, although it is very difficult to work with aromatic hydrocarbons due to their volatility and toxic effects.

Fine Focus ◽  
2016 ◽  
Vol 2 (1) ◽  
pp. 7-14
Author(s):  
Chioma Blaise Chikere ◽  
Chinyere Augusta Ajuzieogu ◽  
Michael Chukwugoziem Miller

Hydrocarbon utilizers are expected to be indigenous in crude-oil polluted environments. The isolation and characterization of hydrocarbon utilizers is often a key strategy in bioremediation of hydrocarbon-polluted environments. In this study, crude-oil polluted soil samples from Obagi town, Onelga, Rivers state were enumerated and characterized for putative hydrocarbon utilizing bacterial populations. Biochemical characterization identified five bacterial species representative of five genera: Bacillus, Pseudomonas, Acinetobacter, Micrococcus and Staphylococcus. Amongst the genera of bacteria isolated, Bacillus had the highest frequency of occurrence (40%). The mean count of total heterotrophic bacteria was 1.7 X 107 cfu/g, while hydrocarbon utilizing bacteria (HUB) count mean density was 1.0 X 107 cfu/g for the three soil samples. Statistical analyses revealed no significant difference at p>0.05 between Total Heterotrophic Bacterial (THB) and Hydrocarbon Utilizing Bacterial (HUB) counts, suggesting that most of the bacteria present in the sampled sites were hydrocarbon utilizers. Findings from this study suggest the presence of indigenous putative hydrocarbon utilizing bacteria in the crude-oil polluted soil of Obagi town. Hence, a promising potential exists for future bioremediation studies on the site.


2020 ◽  
Vol 6 (2) ◽  
pp. 23-28
Author(s):  
O. A. F Wokoma ◽  
◽  
O. S Edori ◽  

Wastewater samples were collected from an oil industry at the point of discharge for a period of two years, from January 2018 – December 2019. The wastewater samples were analyzed for different physicochemical parameters such as temperature, turbidity, total dissolved solids (TDS), total suspended solids (TSS), conductivity, pH, alkalinity, salinity, total hydrocarbon content (THC), biochemical oxygen demand (BOD) and chemical oxygen demand (COD) to examine their conformity to fulfill requirements as recommended by World Health Organization (WHO), Federal Ministry of Environment (FME) and Drinking Water Association (DWA). The results indicated that all the parameters in the discharged wastewater were within acceptable limits of the regulatory bodies. The field data showed that the investigated firm conformed to the law by carrying out proper procedures before discharging the effluents into the public drain and river. Therefore, the release of wastewater from the industry doesn't constitute a danger to the environment as well as aquatic organisms. Keywords: Physicochemical parameter, wastewater discharge, oil industry, environment, contaminants


Author(s):  
Jean-Daniel Hecq ◽  
Benoit Bihin ◽  
Jacques Jamart ◽  
Laurence Galanti

AbstractIn hospitals, a major part of the drugs is administered via the intravenous route. When one wants to evaluate or know the stability of a drug in solution, it is necessary to know several physico-chemical parameters. Several reference works are available to help the hospital pharmacist with this research. However, reading these different sources can make you discover conflicting data. It is therefore necessary at this time to obtain the publications with contradictory results and to read them again. Seven criteria have been identified for judging the quality of a publication on physicochemical stability: full description of equipment, methods and analytical conditions of molecules studied; complete description of the procedures used to validate the analytical method; full indication of time testing and measurement bases or control; documentation on the analytical reproducibility; adequate statistical analysis; appropriate conclusions; appropriate references. In conclusion, everything in a compatibility study is important.


2017 ◽  
Vol 7 (1) ◽  
pp. 47-57
Author(s):  
Ganesh Bahadur Thapa ◽  
Joydeb Pal

Tarahara Fish Farm includes 37 large ponds as a fish super zone. Physicochemical parameters viz., air and water temperature, pH, DO, BOD, CO2, Cl-, TA and TH of these ponds were studied for two years (Nov. 2008 to Oct. 2010). The air temperature had positive and significant correlation with water temperature (r=0.893, P<0.01) but inverse and significant correlation with DO (r = - 0.669 P<0.05) and TH (r = - 0.673, P<0.05). Both air and water temperature showed a decreasing trend during the winter months of November to January and again September to October in both years. Similarly, positive and significant correlation showed by pH with DO (r =0.660, P<0.05) and BOD (r =0.846, P<0.05); by free CO2 with DO (r=0.854, P<0.01), chloride (r=0.648, P<0.05) and TA (r=0.616, P<0.05); by DO with TA (r =0.715, P<0.01) and chloride (r =0.625, P<0.05) and by TH with TA (r= 0.592, P<0.05) but inverse and significant correlation showed by pH with water temperature (r =-0.671, P<0.05); by free CO2 with BOD (r=-0.627, P<0.05) and by DO with BOD (r = -0.810, P <0.01). The water quality parameters were within suitable range for the fish growth and breeding with small fluctuations in different months during the first and second years. Physico-chemical parameters significantly (P<0.01; 0.05) differed between different months. However, no mass mortality of fish was recorded from this site. So, the area selected as fish super zone is fruitful to meet the expected production level of fishes and fish seeds in peripheral areas.


2021 ◽  
Vol 9 (3) ◽  
pp. 039-047
Author(s):  
Edori ES ◽  
Iyama WA ◽  
Awari JO

The levels of physicochemical properties of Mini Whuo Stream in Port Harcourt, Rivers State, Nigeria was evaluated. The physicochemical parameters were analyzed using standard conventional procedures. The average values recorded for the physicochemical parameters during the months of investigation were in the range; temperature; 30.00±0.42-30.26±0.83oC, with all-round mean of 30.14±0.47oC, pH; 6.22±0.33-6.42±0.25 (6.29±0.29), electrical conductivity; 146.01±74.04-147.26±75.13µS/cm (146.58±74.69 µS/cm), total alkalinity; 61.63±6.37-62.20±6.41mg/L (61.91±6.41 mg/L), sulphates; 109.67±51.34-110.67±51.88mg/L (110.22±51.81 mg/L), nitrates; 12.20±2.38-12.60±2.86mg/L (12.31±2.65 mg/L), turbidity; 49.32±1.49-50.18±2.02 NTU(49.83±1.55 NTU), TDS; 74.20±36.43-75.61±37.50 mg/L (75.00±36.81 mg/L), TSS; 27.14±2.12-28.37±0.78mg/L (27.64±1.89 mg/L), chlorides; 15.17±2.90-15.66±2.70mg/L (15.34±2.65 mg/L) and salinity; 24.36±4.83-24.82±4.97mg/L (24.66±4.78 mg/L). The evaluation of the physicochemical parameters showed that the Mini Whuo Stream has been contaminated due to human activities within the stream and therefore adequate measures should be taken to forestall the present situation of the stream in order to mitigate any possible increase in deterioration of the studied physicochemical parameters in the stream beyond acceptable limit.


2017 ◽  
Vol 10 (1) ◽  
pp. 9-14
Author(s):  
ME Huda ◽  
MR Nabi

Appropriate fertilizer and their impact on physico-chemical parameters of water and productivity is very important for aquaculture and ecology. Optimum fertilizer dose can help in fish farmer as well as aquaculture sector. From the study it was found that the total physicochemical parameters of water were suitable for aquaculture, drinking water, irrigation and domestic use. The average water temperature was 26.45±2.75oC; 26.50±3.24oC; 25.83±4.08oC; 26.57±3.02oC and 26.53±2.93oC for MCRT-1 to 5 gradually. Water pH in an average was 7.37±0.61; 7.44±0.55; 7.25±0.58; 7.33±0.54 and 7.47±0.49 for Minature Circular Research Tank (MCRT)-1 to 5 respectively. Average water DO were 6.98±1.05 mgl-1; 6.75±1.53 mgl-1; 6.90±1.64 mgl-1; 6.59±1.19mgl-1 and 6.77±1.60mgl-1 for MCRT-1 to 5 respectively. Average water hardness were 71.88 ± 20.47 mgl-1; 60.5 ±2 1.25 mgl-1; 83.38 ± 23.39 mgl-1; 59.13 ± 25.57 mgl-1 and 52.63 ± 7.92 mgl-1 for MCRT-1 to 5 gradually. Average water total phosphorus were 0.77 ± 0.18 mgl-1; 0.83 ± 0.19 mgl-1; 0.78 ± 0.21 mgl-1; 0.84 ± 0.17 mgl-1 and 0.84 ± 0.16 mgl-1 for MCRT-1 to 5 gradually. From planktonic study it was found that the highest phytoplankton and Zooplankton were in MCRT-3. Phytoplanktons were under 27 no. of genera. Their groups were Cyanophyceae, Chlorophyceae, Bacillariophyceae, Euglenophyceae and Hepatecae.Zooplankton were five major taxa and they were Protozoa, Rotifera, Cladocera, Copepod and Ostracoda respectively.J. Environ. Sci. & Natural Resources, 10(1): 9-14 2017


Biofouling ◽  
1991 ◽  
Vol 4 (1-3) ◽  
pp. 141-150 ◽  
Author(s):  
J J Ten Bosch ◽  
H C Van Der Mei ◽  
H J Busscher

2018 ◽  
Vol 37 ◽  
pp. 01008 ◽  
Author(s):  
Karim Arifi ◽  
Latifa Tahri ◽  
Abdallah El Abid ◽  
Fatima Zahra Hefiane ◽  
Souad Elblidi ◽  
...  

The Grou River is one of the main rivers that feed the reservoir of the Sidi Mohammed Ben Abdellah (SMBA) dam in Morocco. However, this river is particularly threatened by the pollution problem. It is in this context that this study aims to study the effect of wastewater from the rural commune of Jmaa Moulblad on the physicochemical quality of the waters of this river. We analyzed 11 physicochemical parameters (T° of area, T° of water, pH, EC, mV, Sal, TDS, BOD, COD, SS and TOC), with a monthly frequency since the month of December 2014 until November 2015. The results obtained show a worrying situation of the state of this watercourse. To remedy this problem, we recommend pre-treatment of wastewater before discharge and the implementation of a management plan and sustainable management of the Grou River watershed.


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