scholarly journals Antioxidant activity of leaves and bark extracts of Crataeva magna plant

2021 ◽  
Vol 5 (1) ◽  
pp. 001-008
Author(s):  
Faheem I P ◽  
B. Gopalakrishna ◽  
Mohsina FP ◽  
Sarah Priya
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Reducing Power Assay ◽  
Dose Dependent

Recently, a substantial body of literature provides strong evidence to support the potentials of Crataeva magna (CM), plant and its extracts in various pathological conditions. Thus, the CM plant extract at various concentrations were prepared and antioxidant activity was evaluated. The fresh leaves and bark of CM were collected and various extracts such as n-Hexane, Chloroform, Ethanol and aqueous extract were prepared. The preliminary phytochemical investigation showed the presence of various phytochemical such as total phenolic, flavonoid compounds and tannins. The ethanolic leaves extract of CM (ELECM) and water leaves extract of CM (WLECM), showed dose dependent increased in free radical scavenging activity in DPPH assay and reducing power assay in present study. Moreover, ethanolic barks extract of CM (EBECM) and water barks extract of CM (WBECM), also showed increased in free radical scavenging activity in DPPH assay and reducing power assay. However, the effects of bark extracts were lower than leaves extracts. In the light of the above consideration, the results of the study revealed that ELECM showed promising antioxidant activity in a dose dependent manner.

scholarly journals Antioxidant Activity of Moringa oleifera Extracts

2018 ◽  
Vol 16 (3) ◽  
pp. 297 ◽  
Author(s):  
Wiwit Denny Fitriana ◽  
Taslim Ersam ◽  
Kuniyoshi Shimizu ◽  
Sri Fatmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Abts Assay

Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.

scholarly journals Evaluation of antioxidant activity of ormeloxifene: in vitro study

2017 ◽  
Vol 6 (10) ◽  
pp. 2507
Author(s):  
Vinay M. ◽  
Seethalakshmi S. ◽  
Vijay Kumar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
No Synthase ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay

Background: Ormeloxifene (Centchroman) is a Selective Estrogen Receptor Modulator (SERM) which acts as estrogen antagonist and having anti progestogenic activity also. It is being used in the management of dysfunctional uterine bleeding and as nonhormonal oral contraceptive. It is also being investigated for the indications such as osteoporosis, breast and endometrial carcinoma. In this study, we have evaluated the Antioxidant potential of drug by using DPPH and NO synthase Assay. It was found that ormeloxifene has significant antioxidant activity which could be cause for its use in various gynaecological and other conditions.Methods: In this study, we have demonstrated in vitro antioxidant activity of ormeloxifene. DPPH and NO synthase assay tests were done for different concentrations of ormeloxifene.Results: In our study, it showed that the free radical scavenging activity of ormeloxifene was less in lower concentration and increased in the higher concentration in DPPH assay. The free radical scavenging activity of drug ormeloxifene was 22% at 100µg/ml and 27% for the concentrations of 1000µg/ml in DPPH assay. No scavenging activity was 3% at 100µg/ml and 11% at 1000µg/ml.Conclusions: The invitro antioxidant analysis of ormeloxifene, was proved to be a potent antioxidant.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (8) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
iham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (08) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
Siham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel sources of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with a value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels revealed a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, the Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

scholarly journals Antioxidant activity of three different Serbian floral honeys

2011 ◽  
pp. 145-155 ◽  
Author(s):  
Sladjana Savatovic ◽  
Dejan Dimitrijevic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Gordana Cetkovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Content

In this study, three Serbian honey samples (Acacia, Linden and "Homoljski med") were analyzed to determine their total phenolic, flavonoid and antioxidant content, as well as their in vitro antioxidant activity. The antioxidant activity of honeys was examined by different tests, including the reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. In addition, correlations between the antioxidant activity and total phenolic, as well as flavonoid content were also sought. The highest content of total phenolics (27.44 mg/100 g), flavonoids (9.78 mg/100 g), reducing power and DPPH free radical scavenging activity were obtained in the case of Linden honey. The EC50 values of the Linden honey, determined based on the reducing power and DPPH radical scavenging activity, were 24.17 mg/ml and 51.34 mg/ml, respectively. Also, the antioxidant content was highest for Linden honey, and it valued 5.45 mg QEAC/100 g (expressed as mg of quercetin equivalent antioxidant content - QEAC per 100 g of honey) and 7.82 mg AEAC/100 g (expressed as mg of ascorbic acid equivalent antioxidant content - AEAC per 100 g of honey). Also, a linear correlation was observed between the antioxidant activity and total phenolics, as well as total flavonoids.

scholarly journals The Nutritional Composition, Antioxidant Activity and Common Phytochemicals of Selected BARI Mango Varieties and Commercial Cultivar Langra

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
Mohammad Mainuddin Molla ◽  
Ashfak Ahmed Sabuz ◽  
Md. Golam Ferdous Chowdhury ◽  
Md. Hafizul Haque Khan ◽  
Mahfujul Alam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Nutritional Composition ◽  
Free Radical Scavenging ◽  
Anthocyanin Content ◽  
Scavenging Activity

The present study sought to explore the nutritional composition, bioactive phytochemicals and antioxidant activity of BARI mango-4, BARI mango-6 and Langra cultivar. The total phenolic (TPH), vitamin C, total carotene, ß-carotene content and antioxidant activity of the mangos were determined by 1,1- diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays (RPA). Phenolic compounds were assessed using high-performance liquid chromatography coupled with a photodiode array detector and auto sampler. Results revealed that moisture, TSS, pH, total acidity, reducing, total sugar and energy of the BARI mango-4 and BARI mango-6 were 76.54 and 75.24 %, 17.10°B and 21.20°B, 4.90 and 5.01, 0.49 and 0.50 %, 3.90 and 4.54 %, 11.20 and 13.46 % and 4028.06 and 3950.27 cal/g respectively whereas the Langra cultivar remained 76.33 %, 17.63°B, 4.25, 0.63 %, 2.79 %, 9.79 % % and 3871.28 cal/g respectively. Phytochemicals especially TPH, ascorbic acid, total flavonoid (TF), total carotenoid (TC), ß-carotene and total anthocyanin content (TAC) of the BARI mango-4 and BARI mango-6 were 20.53 and 20.67 mg GAE/g, 39.98 and 26.26 mg/100 g, 3.14 mg and 2.87 QE/g, 76.38 and 81.33 mg/100 g, 28.17 and 65.84 µg/100 g and 1.67 and 11.69 mg/100 respectively whereas the Langra contained 19.90 mg GAE/g, 25.53 mg/100g, 1.38 mg QE/g, 4.21 mg/100 g, 31.00 µg/100 g and18.22 mg/100 g respectively. In case of antioxidant activities total antioxidant capacity, DPPH radical scavenging activity, reducing power capacity (RPC), metal chelating capacity (MCC), Nitric oxide (NO) free radical scavenging activity and IC50 of the BARI mango-4 and BARI mango-6 were 229.00 and 309.00 µg of ascorbic acid/mg of extract, 96.84 and 94.73 %, 12.20 and 9.71 µg/mL, 157.36 and 132.89 %, 61.74 and 72.65 µg/mL and 0.59 and 0.71 µg/mL respectively whereas the Langra cultivar contained 194.25 µg of ascorbic acid/mg of extract, 87.94 %, 2.54 µg/mL, 177.80 %, 53.74 µg/mL and 25.11 µg/mL respectively. The results indicate that BARI mango-4 and BARI mango-6 exhibited rich source of TPH, TC, ß-carotene, ascorbic acid, TA, TAC and NO free radical scavenging activity whereas the Langra is the rich source of MCC and anthocyanin content. Phenolic acids were leading agent in BARI mango-4 and BARI mango-6. Moreover, BARI mango-4 and BARI mango-6 extract had a great potential to fight free radical chain reactions and for usage in therapeutic applications.

scholarly journals Free radical scavenging ability of a potent therapeutic plant mussaenda luteola delile (rubiaceae)

2021 ◽  
Vol 8 (1) ◽  
pp. 69-72
Author(s):  
Betty T ◽  
Sumathi P ◽  
Indhumathi T ◽  
Prabavathi B ◽  
Devadharshini B
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Leaf Extracts

The aim of present study was to investigate the in vitro antioxidant potential and total extractive yield of Mussaenda luteola Delile leaves. Antioxidant activity was assessed by using 2,2- diphenyl-1-picryl-hydrazyl (DPPH• ) assay, reducing power activity and [2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)] ABTS•+ assay. Here ascorbic acid (ASA) and rutin were used as standard antioxidants. The results of the study indicates that the chloroform extracts of the leaf of Mussaenda luteola possesses significant scavenging activity against DPPH• (17.56) and reducing power activity (0.759) at 700nm absorbance. The ethanolic leaf extracts holds high free radical scavenging activity (ABTS•+) at 735nm (94.59). The free radical scavenging and antioxidant activities may be attributed to the presence of adequate phenolic and flavonoid compounds. The ethanolic leaf extract of M. luteola yields maximum extractive yield percentage (37.08%). This study revealed that the leaf extracts of Mussaenda luteola has demonstrated significant antioxidant activity.

scholarly journals QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ELYTRARIA ACAULIS LINDAU (ACANTHACEAE) Research Article

2016 ◽  
Vol 9 (9) ◽  
Author(s):  
Manigandan Murugan ◽  
Kolanjinathan K
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Reducing Power Assay

ABSTRACTObjectives: To analyze the phytochemicals quantitatively and screening the antioxidant efficacy of methanol extracts of Elytraria acaulis plant extractby in vitro.Methods: The total phenols, flavonoids, and tannin contents analyzed by Folin-Ciocalteu method, aluminum chloride colorimetric and calculated asgallic acid equivalents/g (GAE/g) of dry weight. Free radical scavenging activity was screened using 1,1-diphenyl-2-picrylhydrazyl (DPPH), radicalscavenging activities, and ferric reducing power assay was also analyzed.Results: The total phenolic content of the aqueous leaf extract was 46.84 µg GAE/g of extract powder. Significantly similar levels of total flavonoid andtannin contents of the plant were 41.72 and 39.50 µg GAE/g, respectively. The plant extracts showed appreciable free radical scavenging activities atthe highest concentration of 400 µg/mL superoxide anion radical with IC values (107.7±1.081 µg/mL), and for DPPH and considerably high amountof radical scavenging activity was found with very low IC5050 values (4.3±0.88 µg/mL) compared with quercetin.Conclusion: The phenolic and flavonoid compounds provide substantial antioxidant properties which could be effectively used for pharmaceutical,nutraceutical as well as anti-inflammatory applications.Keywords: Phenols, Flavonoids, 1,1-diphenyl-2-picrylhydrazyl, Pharmaceuticals.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

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