scholarly journals The Nutritional Composition, Antioxidant Activity and Common Phytochemicals of Selected BARI Mango Varieties and Commercial Cultivar Langra

Author(s):  
Mohammad Mainuddin Molla ◽  
Ashfak Ahmed Sabuz ◽  
Md. Golam Ferdous Chowdhury ◽  
Md. Hafizul Haque Khan ◽  
Mahfujul Alam ◽  
...  

The present study sought to explore the nutritional composition, bioactive phytochemicals and antioxidant activity of BARI mango-4, BARI mango-6 and Langra cultivar. The total phenolic (TPH), vitamin C, total carotene, ß-carotene content and antioxidant activity of the mangos were determined by 1,1- diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays (RPA). Phenolic compounds were assessed using high-performance liquid chromatography coupled with a photodiode array detector and auto sampler. Results revealed that moisture, TSS, pH, total acidity, reducing, total sugar and energy of the BARI mango-4 and BARI mango-6 were 76.54 and 75.24 %, 17.10°B and 21.20°B, 4.90 and 5.01, 0.49 and 0.50 %, 3.90 and 4.54 %, 11.20 and 13.46 % and 4028.06 and 3950.27 cal/g respectively whereas the Langra cultivar remained 76.33 %, 17.63°B, 4.25, 0.63 %, 2.79 %, 9.79 % % and 3871.28 cal/g respectively. Phytochemicals especially TPH, ascorbic acid, total flavonoid (TF), total carotenoid (TC), ß-carotene and total anthocyanin content (TAC) of the BARI mango-4 and BARI mango-6 were 20.53 and 20.67 mg GAE/g, 39.98 and 26.26 mg/100 g, 3.14 mg and 2.87 QE/g, 76.38 and 81.33 mg/100 g, 28.17 and 65.84 µg/100 g and 1.67 and 11.69 mg/100 respectively whereas the Langra contained 19.90 mg GAE/g, 25.53 mg/100g, 1.38 mg QE/g, 4.21 mg/100 g, 31.00 µg/100 g and18.22 mg/100 g respectively. In case of antioxidant activities total antioxidant capacity, DPPH radical scavenging activity, reducing power capacity (RPC), metal chelating capacity (MCC), Nitric oxide (NO) free radical scavenging activity and IC50 of the BARI mango-4 and BARI mango-6 were 229.00 and 309.00 µg of ascorbic acid/mg of extract, 96.84 and 94.73 %, 12.20 and 9.71 µg/mL, 157.36 and 132.89 %, 61.74 and 72.65 µg/mL and 0.59 and 0.71 µg/mL respectively whereas the Langra cultivar contained 194.25 µg of ascorbic acid/mg of extract, 87.94 %, 2.54 µg/mL, 177.80 %, 53.74 µg/mL and 25.11 µg/mL respectively. The results indicate that BARI mango-4 and BARI mango-6 exhibited rich source of TPH, TC, ß-carotene, ascorbic acid, TA, TAC and NO free radical scavenging activity whereas the Langra is the rich source of MCC and anthocyanin content. Phenolic acids were leading agent in BARI mango-4 and BARI mango-6. Moreover, BARI mango-4 and BARI mango-6 extract had a great potential to fight free radical chain reactions and for usage in therapeutic applications.

2011 ◽  
Vol 396-398 ◽  
pp. 1645-1651 ◽  
Author(s):  
Qiu Ping Zhong ◽  
Yong Cheng Li

In this study, total polyphenols content (TPC) and ascorbic acid content (AAC) in CAW were quantified. Total antioxidant activity, reducing capacity, and free radical scavenging activity of CAW were assayed. The TPC and AAC were 160 mg GAE /100ml and 132 mg/100 ml,respectivity. CAW exhibited effective antioxidant activities in the employed in vitro experiments, including total antioxidant activity, reducing capacity, and free radical scavenging activity, which indicates that phenolic compounds and ascorbic acid may play a key role in the antioxidant function of CAW.


2021 ◽  
Vol 5 (1) ◽  
pp. 001-008
Author(s):  
Faheem I P ◽  
B. Gopalakrishna ◽  
Mohsina FP ◽  
Sarah Priya

Recently, a substantial body of literature provides strong evidence to support the potentials of Crataeva magna (CM), plant and its extracts in various pathological conditions. Thus, the CM plant extract at various concentrations were prepared and antioxidant activity was evaluated. The fresh leaves and bark of CM were collected and various extracts such as n-Hexane, Chloroform, Ethanol and aqueous extract were prepared. The preliminary phytochemical investigation showed the presence of various phytochemical such as total phenolic, flavonoid compounds and tannins. The ethanolic leaves extract of CM (ELECM) and water leaves extract of CM (WLECM), showed dose dependent increased in free radical scavenging activity in DPPH assay and reducing power assay in present study. Moreover, ethanolic barks extract of CM (EBECM) and water barks extract of CM (WBECM), also showed increased in free radical scavenging activity in DPPH assay and reducing power assay. However, the effects of bark extracts were lower than leaves extracts. In the light of the above consideration, the results of the study revealed that ELECM showed promising antioxidant activity in a dose dependent manner.


Author(s):  
Makhlouf Chaalal ◽  
iham Ydjedd ◽  
Sana Mansouri

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.


Author(s):  
Makhlouf Chaalal ◽  
Siham Ydjedd ◽  
Sana Mansouri

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel sources of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with a value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels revealed a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, the Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.


2011 ◽  
pp. 145-155 ◽  
Author(s):  
Sladjana Savatovic ◽  
Dejan Dimitrijevic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Gordana Cetkovic ◽  
...  

In this study, three Serbian honey samples (Acacia, Linden and "Homoljski med") were analyzed to determine their total phenolic, flavonoid and antioxidant content, as well as their in vitro antioxidant activity. The antioxidant activity of honeys was examined by different tests, including the reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. In addition, correlations between the antioxidant activity and total phenolic, as well as flavonoid content were also sought. The highest content of total phenolics (27.44 mg/100 g), flavonoids (9.78 mg/100 g), reducing power and DPPH free radical scavenging activity were obtained in the case of Linden honey. The EC50 values of the Linden honey, determined based on the reducing power and DPPH radical scavenging activity, were 24.17 mg/ml and 51.34 mg/ml, respectively. Also, the antioxidant content was highest for Linden honey, and it valued 5.45 mg QEAC/100 g (expressed as mg of quercetin equivalent antioxidant content - QEAC per 100 g of honey) and 7.82 mg AEAC/100 g (expressed as mg of ascorbic acid equivalent antioxidant content - AEAC per 100 g of honey). Also, a linear correlation was observed between the antioxidant activity and total phenolics, as well as total flavonoids.


2021 ◽  
Vol 8 (1) ◽  
pp. 69-72
Author(s):  
Betty T ◽  
Sumathi P ◽  
Indhumathi T ◽  
Prabavathi B ◽  
Devadharshini B

The aim of present study was to investigate the in vitro antioxidant potential and total extractive yield of Mussaenda luteola Delile leaves. Antioxidant activity was assessed by using 2,2- diphenyl-1-picryl-hydrazyl (DPPH• ) assay, reducing power activity and [2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)] ABTS•+ assay. Here ascorbic acid (ASA) and rutin were used as standard antioxidants. The results of the study indicates that the chloroform extracts of the leaf of Mussaenda luteola possesses significant scavenging activity against DPPH• (17.56) and reducing power activity (0.759) at 700nm absorbance. The ethanolic leaf extracts holds high free radical scavenging activity (ABTS•+) at 735nm (94.59). The free radical scavenging and antioxidant activities may be attributed to the presence of adequate phenolic and flavonoid compounds. The ethanolic leaf extract of M. luteola yields maximum extractive yield percentage (37.08%). This study revealed that the leaf extracts of Mussaenda luteola has demonstrated significant antioxidant activity.


Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents


2014 ◽  
Vol 68 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Jelica Stojanovic ◽  
Slavica Solujic ◽  
Miroslav Vrvic

The aim of this study was to investigate the biological and chemical activity on two species of fungi of the genus Penicillium isolated from wastewater. On the selected species of fungi the different antioxidant activity assays were carried out: DPPH free-radical scavenging activity, total antioxidant activity, Fe2+- chelating ability and Fe3+- reducing power. Total phenol content was also determinate for ethanolic extract of mycelia. Penicillium chrysogenum ethanolic extract contained higher total phenolic content and better total antioxidant capacity as well as ferrous ion chelating ability. Penicillium fumiculosum ethanolic extract showed higher DPPH free-radical scavenging activity, as well as reducing power. Based on the obtained results it can be concluded that two types of fungi are potential new sources of natural antioxidants.


2018 ◽  
Vol 38 (2) ◽  
pp. 200
Author(s):  
Paini Sri Widyawati ◽  
Tarsisius Dwi Wibawa Budianta ◽  
Yesiana Dwi Wahyu Werdani ◽  
Maria Olivia Halim

The research was conducted to explore the potency of pluchea leaves-black tea drink as antioxidant at various proportions. The research used a single factor randomized block design of pluchea leaves and black tea proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w). Each of it was repeated five times. The parameters observed in this study were secondary metabolites, total phenolic, total flavonoids, free radical DPPH scavenging activity, and iron reducing power. The data were statistically analyzed using Analysis of Variance (ANOVA) at α = 5%, if the analysis showed a significant effect then it was continued with Duncan's Multiple Range Test (DMRT). Data was stated as mean ± standard deviation. The results showed that the secondary metabolites containing in drink from pluchea leaves and black tea at various proportions were alkaloids, flavonoids, phenolics, saponins, tannins, and cardiac glycosides. The increasing of black tea proportion in samples added the intensity of alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides compounds detected, but the tannins were decreased. These secondary metabolites were correlated with total phenolic content (TPC) and total flavonoid content (TFC). The increasing of black tea proportion in drink significantly decreased DPPH free radical scavenging activity and iron ion reducing power, except for 100% black tea proportion. Tannin compounds seems determining antioxidant activity.  Based on coefficient correlation between TPC or TFC and DPPH free radical scavenging activity or iron ion reducing power, the result showed that DPPH free radical scavenging activity was dominantly contributed by TPC and iron ion reducing power was determined by TPC and TFC. ABSTRAKPenelitian ini dilakukan untuk mengetahui potensi minuman daun beluntas teh hitam sebagai antioksidan pada berbagai proporsi. Penelitian ini menggunakan rancangan acak kelompok satu faktor, yaitu proporsi daun beluntas teh hitam meliputi 100:0; 75:25; 50:50; 25:75; dan 0:100% (b/b). Tiap faktor diulang sebanyak 5 kali. Parameter yang diamati pada penelitian ini meliputi kandungan metabolit sekunder, total fenolik (TPC), total flavonoid (TFC), kemampuan menangkal radikal bebas DPPH, dan kemampuan mereduksi ion besi. Data dianalisis secara statistik dengan Analysis of Variance (ANOVA) pada α = 5%, jika terdapat beda signifikan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Data dinyatakan dengan rata-rata ± standar deviasi.  Hasil menunjukkan bahwa metabolit sekunder yang terkandung dalam minuman daun beluntas teh hitam pada berbagai proporsi adalah alkaloid, flavonoid, fenolik, saponin, tannin, dan kardiak glikosida. Peningkatan proporsi teh hitam menambah intensitas senyawa alkaloid, fenolik, flavonoid, saponin, dan kardiak glikosida yang terdeteksi, tetapi kandungan senyawa tannin berkurang. Kandungan metabolit sekunder ini berkorelasi dengan total fenolik (TPC) dan total flavonoid (TFC). Peningkatan proporsi teh hitam dalam minuman menurunkan kemampuan menangkal radikal bebas DPPH dan kemampuan mereduksi ion besi, kecuali pada proporsi teh hitam 100%. Kandungan senyawa tannin dalam minuman menentukan aktivitas antioksidan. Berdasarkan koefisien korelasi antara TPC atau TFC dan kemampuan menangkal radikal bebas DPPH atau kemampuan mereduksi ion besi, hasil menunjukkan bahwa kemampuan menangkal radikal bebas DPPH dominan dikontribusi oleh TPC dan kemampuan mereduksi ion besi ditentukan oleh TPC dan TFC.


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