scholarly journals Pengaruh Pemberian Naungan Terhadap Aklimatisasi Planlet Strowberi Varietas Dorit dan Varietas Lokal Berastagi

2021 ◽  
Vol 2 ◽  
pp. 228-233
Author(s):  
Hasim Ashari ◽  
Zainuri Hanif
Keyword(s):  
Tissue Culture ◽  
Vegetative Growth ◽  
Climate Data ◽  
Strawberry Plant ◽  
Local Varieties ◽  
Culture Techniques ◽  
Number Of Leaves ◽  
Growing Environment ◽  
Housing Needs ◽  
Tissue Culture Techniques

Strawberry plant tissue culture techniques have been successful in plantlet propagation. In order to produce strawberry seeds that are ready for planting, plantlets must go through the acclimatization stage. A modified growing environment with a plastic hood or housing needs to be considered to provide optimal environmental conditions for the acclimatization of strawberry plantlets. This study aimed to describe the effect of shading and type of variety on plantlet acclimatization of the Dorit variety and the local Berastagi variety. This research was experimental with RAK using 2 factors, namely shade with the plastic thickness of 0.17 mm and 0.12 mm and types of local varieties of Berastagi and Dorit varieties. Parameters observed were climate data, vegetative growth (percentage of plantlets growing, number of stolons, diameter of stolons, number of leaves). The data were analyzed with Anova 2 factors; if the results were significant, it would be continued with Duncan's test. The results showed that the shade had a significant effect on the growth of strawberry plantlets. In contrast, the variety had a significant effect on the vegetative growth of strawberries, and the variety with the best growth was the Berastagi variety.

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals Enhancing Micro-propagation of Some Cameroonian Plantain (Musa paradisiaca L) Cultivars/Landraces

2019 ◽  
pp. 1-10
Author(s):  
Dominic, K. Njualem ◽  
Tange Denis Achiri ◽  
Amanwi Climson Yuninwenkeh ◽  
Abdulai Assan Nkuh ◽  
Fornkwa Victorine Yaya
Keyword(s):  
Tissue Culture ◽  
Agricultural Research ◽  
Germplasm Conservation ◽  
Culture Techniques ◽  
Shoot Initiation ◽  
Randomized Design ◽  
Micro Propagation ◽  
Increase Productivity ◽  
Number Of Leaves ◽  
Tissue Culture Techniques

Aims: This study was conducted to enhance germplasm conservation of some indigenous plantain landraces through tissue culture techniques in Cameroon. Study Design: The experiment was laid out in a completely randomized design (CRD) with three treatments in four replications. Place and Duration of Study: The study was conducted in the tissue culture laboratory of the Institute of Agricultural Research for Development (IRAD), Bambui, Cameroon, in the first half of 2018. Methodology: Explants were gotten from three plantain land races (Kwah, Ngumbe and Sangmoh) gotten from Bambui. Shoot tips were excised and cultured on Murashige and Skoog (MS) medium supplemented with 30 g of sucrose, 5 ml of ascorbic acid, 4 ml of 6- benzylaminopurine (BAP 1 mg/l), 1 ml indole-3- acetic acid (IAA 1 mg/l) and 6 g of agar at pH of 5.8±0.1 for shoot initiation and proliferation. Data was collected over a period of 12 weeks every 4th week on the average number of buds, shoot length, number of leaves, number contaminated and number dead. Results: All the landraces responded positively to shoot tip culture, since they could adapt and regenerate by producing buds with Ngumbe giving the highest mean number (7.0) of buds and Sangmoh giving the lowest (3.0). Analysis of variance revealed significant differences (p = 0.05) in most of the parameters measured except for number contaminated. High dead rate was recorded in Kwah with dead mean of 3.0. Ngumbe was found to regenerate better than Kwah and Sangmoh. Conclusion: It is recommended that the three plantain landraces, germplasm be conserved. The ramification of this finding vis-à-vis germplasm conservation and increase productivity is explained.

scholarly journals Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam

2021 ◽  
Vol 19 (3) ◽  
pp. 66-70
Author(s):  
Muhklisani ◽  
Panca Dewi Manuhara Karti ◽  
Iwan Prihantoro
Keyword(s):  
Tissue Culture ◽  
Leucaena Leucocephala ◽  
Culture Techniques ◽  
Best Response ◽  
Randomized Design ◽  
Culture Production ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Mutant Lines ◽  
Tissue Culture Techniques

Acclimatization is the final stage of plant propagation in tissue culture techniques that can determine the success of the nursery process. This study aimed to observe the growth response during the acclimatization stage of the acid-adapted of Leucaena leucocephala Tarramba variety, which developed from tissue culture techniques. The design used in this study was a completely randomized design (CRD) with the cultivation of 11 mutant lines from tissue culture, namely M1-M11 (an acid-adapted mutant from tissue culture addition of 1 ppm IBA) and 2 controls (lamtoro broodstock without gamma irradiation) namely K0 (lamtoro broodstock resulting from tissue culture addition of 0 ppm IBA), K1 (breeding lamtoro from tissue culture addition of 1 ppm IBA). The variables observed were the level of plant viability, plant height, and number of leaves. The results showed that the acclimatization of the plant Leucaena leucocephala to tissue culture production on the M3 and M9 mutant lines gave the best response to plant morphological growth up to 5 WAP (weeks after planting). Key words:        acclimatization, IBA hormone, Leucaena leucocephala, tissue culture

scholarly journals Multiplikasi Anggrek Hitam ( Coelogyne pandurata Lindl ) Pada Media Murashige Skoog (Ms) Dengan Penambahan Ekstrak Pisang Ambon dan Benzyl Amino Purin (BAP)

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Nurfadilah Mukarlina Elvi Rusmiyanto P.W
Keyword(s):  
Tissue Culture ◽  
Aloe Vera ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Murashige Skoog ◽  
Number Of Leaves ◽  
Habitat Damage ◽  
Over Exploitation ◽  
Tissue Culture Techniques

Black orchids (Coelogyne pandurata Lindl.) are epiphytic and endemic in Borneo. Habitat damage and over exploitation by the society can cause black orchids to be threatened with extinction. For this reason, alternative ways to increase black orchids are needed through tissue culture techniques with the addition of ambon banana extract and Benzyl Amino Purine BAP. The aim of this study was to determine the effect of ambon banana extract and BAP on the multiplication of black orchids. This research was conducted for 3 months from January to March 2018 at Pontianak Aloe Vera Center (AVC) Tissue Culture Laboratory. This study uses a Completely Randomized Design (CRD) with two factors. The first factor was ambon banana extract (0%; 2,5%; 5%; 7,5% and 10%) and the second factors BAP (0 M; 10-7M; 10-6M; 5x10-6M) with 3 replications, each of which obtained 60 trial unit. The results showed that the ambon banana extract had a significant effect on the time of budding, number of buds and number of leaves. The concentration of ambon banana extract 2,5% resulted in the fastest budding time appearing on the 11,33th day, the number of buds was 8,33 tillers, and the number of leaves was 17,67 strands.

Cytogenetic Analysis of 39 Prostate Carcinomas and Evaluation of Short Term Tissue Culture Techniques

1998 ◽  
Vol 101 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Josée J König ◽  
Wilma Teubel ◽  
Eric Kamst ◽  
Johannes C Romijn ◽  
Fritz H Schröder ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cytogenetic Analysis ◽  
Short Term ◽  
Culture Techniques ◽  
Prostate Carcinomas ◽  
Tissue Culture Techniques

DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

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