scholarly journals Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam

2021 ◽  
Vol 19 (3) ◽  
pp. 66-70
Author(s):  
Muhklisani ◽  
Panca Dewi Manuhara Karti ◽  
Iwan Prihantoro
Keyword(s):  
Tissue Culture ◽  
Leucaena Leucocephala ◽  
Culture Techniques ◽  
Best Response ◽  
Randomized Design ◽  
Culture Production ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Mutant Lines ◽  
Tissue Culture Techniques

Acclimatization is the final stage of plant propagation in tissue culture techniques that can determine the success of the nursery process. This study aimed to observe the growth response during the acclimatization stage of the acid-adapted of Leucaena leucocephala Tarramba variety, which developed from tissue culture techniques. The design used in this study was a completely randomized design (CRD) with the cultivation of 11 mutant lines from tissue culture, namely M1-M11 (an acid-adapted mutant from tissue culture addition of 1 ppm IBA) and 2 controls (lamtoro broodstock without gamma irradiation) namely K0 (lamtoro broodstock resulting from tissue culture addition of 0 ppm IBA), K1 (breeding lamtoro from tissue culture addition of 1 ppm IBA). The variables observed were the level of plant viability, plant height, and number of leaves. The results showed that the acclimatization of the plant Leucaena leucocephala to tissue culture production on the M3 and M9 mutant lines gave the best response to plant morphological growth up to 5 WAP (weeks after planting). Key words:        acclimatization, IBA hormone, Leucaena leucocephala, tissue culture

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

2018 ◽  
Vol 4 (2) ◽  
pp. 60
Author(s):  
Asgar Taiyeb ◽  
Baharuddin Baharuddi
Keyword(s):  
Tissue Culture ◽  
Culture Techniques ◽  
In Vitro Multiplication ◽  
Supportive Environment ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
The Difference ◽  
Tissue Culture Techniques

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of  Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to  in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.

scholarly journals Enhancing Micro-propagation of Some Cameroonian Plantain (Musa paradisiaca L) Cultivars/Landraces

2019 ◽  
pp. 1-10
Author(s):  
Dominic, K. Njualem ◽  
Tange Denis Achiri ◽  
Amanwi Climson Yuninwenkeh ◽  
Abdulai Assan Nkuh ◽  
Fornkwa Victorine Yaya
Keyword(s):  
Tissue Culture ◽  
Agricultural Research ◽  
Germplasm Conservation ◽  
Culture Techniques ◽  
Shoot Initiation ◽  
Randomized Design ◽  
Micro Propagation ◽  
Increase Productivity ◽  
Number Of Leaves ◽  
Tissue Culture Techniques

Aims: This study was conducted to enhance germplasm conservation of some indigenous plantain landraces through tissue culture techniques in Cameroon. Study Design: The experiment was laid out in a completely randomized design (CRD) with three treatments in four replications. Place and Duration of Study: The study was conducted in the tissue culture laboratory of the Institute of Agricultural Research for Development (IRAD), Bambui, Cameroon, in the first half of 2018. Methodology: Explants were gotten from three plantain land races (Kwah, Ngumbe and Sangmoh) gotten from Bambui. Shoot tips were excised and cultured on Murashige and Skoog (MS) medium supplemented with 30 g of sucrose, 5 ml of ascorbic acid, 4 ml of 6- benzylaminopurine (BAP 1 mg/l), 1 ml indole-3- acetic acid (IAA 1 mg/l) and 6 g of agar at pH of 5.8±0.1 for shoot initiation and proliferation. Data was collected over a period of 12 weeks every 4th week on the average number of buds, shoot length, number of leaves, number contaminated and number dead. Results: All the landraces responded positively to shoot tip culture, since they could adapt and regenerate by producing buds with Ngumbe giving the highest mean number (7.0) of buds and Sangmoh giving the lowest (3.0). Analysis of variance revealed significant differences (p = 0.05) in most of the parameters measured except for number contaminated. High dead rate was recorded in Kwah with dead mean of 3.0. Ngumbe was found to regenerate better than Kwah and Sangmoh. Conclusion: It is recommended that the three plantain landraces, germplasm be conserved. The ramification of this finding vis-à-vis germplasm conservation and increase productivity is explained.

scholarly journals The Effect of Kinetin Concentration on Eucalyptus pellita F. Muell Micro Cutting Growth (In Vitro)

2019 ◽  
Vol 1 (1) ◽  
pp. 34
Author(s):  
Ridho Dzikrana
Keyword(s):  
Leaf Color ◽  
Culture Techniques ◽  
Eucalyptus Pellita ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Single Factor Experiment ◽  
Tissue Culture Techniques

Propagation of quality, uniform, and large quantities of Eucalyptus pellita F. Muell seeds as one type of industrial forest plant can be done by tissue culture techniques.  The aim of the study was to determine: 1) the effect of  kinetin concentration on the growth of E. pellita micro cuttings; 2) kinetin concentration which gives the best effect on the growth of E. pellitamicro cuttings.  A single factor experiment, kinetin concentration, was arranged in a Completely Randomized Design (CRD), consisted of five treatments, namely 0; 1; 2; 3; and 4 mg of kinetin L-1 and each treatment was replicated five times.  The variables observed consisted of plant height, leaf color, and number of leaves.  Data were analyzed by analysis of variance (anova) and if the anova was significantly different, to compare between the two treatment averages, followed by a Least Significant Difference  (LSD) test at the level of 5%.  The results showed that different kinetin concentrations had different effects on the height of the culture and number of leaves, but the color of the leaves did not show significant different.  A concentration of  3 mg of kinetin L-1 gave the best effect on the height cuttings at all observation ages: 7; 14; 21; 28; 35; 42; and 49 days after inoculation (DAI) and number of leaves.

scholarly journals Multiplikasi Anggrek Hitam ( Coelogyne pandurata Lindl ) Pada Media Murashige Skoog (Ms) Dengan Penambahan Ekstrak Pisang Ambon dan Benzyl Amino Purin (BAP)

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Nurfadilah Mukarlina Elvi Rusmiyanto P.W
Keyword(s):  
Tissue Culture ◽  
Aloe Vera ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Murashige Skoog ◽  
Number Of Leaves ◽  
Habitat Damage ◽  
Over Exploitation ◽  
Tissue Culture Techniques

Black orchids (Coelogyne pandurata Lindl.) are epiphytic and endemic in Borneo. Habitat damage and over exploitation by the society can cause black orchids to be threatened with extinction. For this reason, alternative ways to increase black orchids are needed through tissue culture techniques with the addition of ambon banana extract and Benzyl Amino Purine BAP. The aim of this study was to determine the effect of ambon banana extract and BAP on the multiplication of black orchids. This research was conducted for 3 months from January to March 2018 at Pontianak Aloe Vera Center (AVC) Tissue Culture Laboratory. This study uses a Completely Randomized Design (CRD) with two factors. The first factor was ambon banana extract (0%; 2,5%; 5%; 7,5% and 10%) and the second factors BAP (0 M; 10-7M; 10-6M; 5x10-6M) with 3 replications, each of which obtained 60 trial unit. The results showed that the ambon banana extract had a significant effect on the time of budding, number of buds and number of leaves. The concentration of ambon banana extract 2,5% resulted in the fastest budding time appearing on the 11,33th day, the number of buds was 8,33 tillers, and the number of leaves was 17,67 strands.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽  
2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Ega Raisya ◽  
Denny Sobardini Sobarna ◽  
Anne Nuraini ◽  
Syariful Mubarok ◽  
Erni Suminar ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fast Time ◽  
Endogenous Auxin ◽  
Seed Technology ◽  
Randomized Design ◽  
Process Plants ◽  
Number Of Leaves ◽  
Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm  berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

scholarly journals Pengaruh Konsentrasi Jus Lidah Buaya (Aloe cbinensis Baker) Terhadap Pertumbuhan Planlet Anggrek Hasil Silangan Dendrobium Morning Sun X Dendrobium Samarai

OrchidAgro ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Reo Sempana ◽  
Lia Amalia ◽  
Wahyono Widodo ◽  
Elly Roosma Ria ◽  
Noertjahyani Noertjahyani
Keyword(s):  
Tissue Culture ◽  
Experimental Design ◽  
Root Length ◽  
Aloe Vera ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
C 50 ◽  
Better Than ◽  
Number Of Shoots

This experiment aims was to study the effect of the concentration of aloe vera juice and to get the concentration of aloe vera juice which shows better growth of the number of leaves and the number of roots on the Dendrobium orchid plantlet in Hybrid-vitro results. This experiment was carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Winaya Mukti University, which began in May to August 2019. The experimental design was used a Completely Randomized Design with 5 treatments in 5 replications and the treatments namely: A (0 g L-1 solution), B (25 g L-1 solution), C (50 g L-1 solution), D (75 g   L-1 solution), E (100 g L-1 solution). The results showed that consentration of B (25 g L-1 solution), C (50 g L-1 solution), D (75 g   L-1 solution) gave plantlet height, number of shoots, number of roots, and root length better than another treatments.

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