scholarly journals Towards universal detection of Luteoviridae

2009 ◽  
Vol 62 ◽  
pp. 407-407
Author(s):  
A. Chomic ◽  
M.N. Pearson ◽  
K.F. Armstrong
Keyword(s):  
New Zealand ◽  
Plant Virus ◽  
Universal Primers ◽  
High Homology ◽  
Yield Losses ◽  
Virus Family ◽  
Low Concentration ◽  
Conserved Regions ◽  
Universal Detection ◽  
Pcr Conditions

Luteoviridae is a plant virus family of 26 species which causes yield losses in cereals potatoes and other economically important crops worldwide Accurate detection is an important component of controlling the spread of luteoviruses and reducing yield losses PCRbased detection is often the method of choice for Luteoviridae as it is more sensitive than serological methods which frequently fail to detect infection due to the low concentration of luteoviruses in plants Since the currently available luteovirus primers are mostly speciesspecific and work under different PCR conditions universal primers are desirable Seven primers were tested that were designed to target the most conserved regions of the Luteoviridae genomes and that possess high homology to over 75 of Luteoviridae species Thirty luteovirus isolates representing 15 species were obtained from New Zealand and overseas these included all five species from the Luteovirus genus eight of the nine species from the Polerovirus genus (except CYDV RPS) PEMV1 (the only species from the Enamovirus genus) and CtRLV (which is not assigned to a genus) Between them the three combinations of primers detected all 13 of the Luteovirus and Polerovirus species tested

Fertiliser practice and yield losses in process potato crops grown in Canterbury, New Zealand

2016 ◽  
Vol 44 (1) ◽  
pp. 41-57 ◽  
Author(s):  
JB Reid ◽  
BP Searle ◽  
SM Sinton ◽  
A Michel ◽  
ED Meenken ◽  
...  
Keyword(s):  
New Zealand ◽  
Yield Losses ◽  
Potato Crops

A review of the plant virus, viroid and mollicute records for New Zealand

2006 ◽  
Vol 35 (2) ◽  
pp. 217 ◽  
Author(s):  
M. N. Pearson ◽  
G. R. G. Clover ◽  
P. L. Guy ◽  
J. D. Fletcher ◽  
R. E. Beever
Keyword(s):  
New Zealand ◽  
Plant Virus

scholarly journals Survey of blueberry farms for Botryosphaeria dieback and crown rot pathogens

2009 ◽  
Vol 62 ◽  
pp. 238-242 ◽  
Author(s):  
J. Sammonds ◽  
R. Billones ◽  
H.J. Ridgway ◽  
M. Walter ◽  
M.V. Jaspers
Keyword(s):  
New Zealand ◽  
Molecular Identification ◽  
Crown Rot ◽  
Yield Losses ◽  
Morphological Examination ◽  
Stem Lesions ◽  
Production Areas

Dieback and crown rot affect about 18 of blueberry plants in the main New Zealand production areas costing about 500000 annually due to yield losses and replanting costs Samples of symptomatic plants and stems were collected from six blueberry farms in the central North Island to determine which pathogens were responsible From the 70 plants collected numerous isolations from branch bases and tips stem lesions and crowns revealed the apparent presence of Botryosphaeria species in 69 645 543 and 70 of samples respectively These Botryosphaeria spp were also found in a few roots and leaves but not fruit from symptomatic plants nor in healthy asymptomatic stems Morphological examination of conidia from 40 cultures identified B lutea B parva B lutea/australis and B obtusa with one unidentified Botryosphaeria species apparently of the Neofusicoccum type Molecular identification of 14 representative isolates from these 40 confirmed the presence of B lutea B parva and B australis

A review of the plant virus, viroid, liberibacter and phytoplasma records for New Zealand

2015 ◽  
Vol 44 (5) ◽  
pp. 463-514 ◽  
Author(s):  
S. Veerakone ◽  
J. Z. Tang ◽  
L. I. Ward ◽  
L. W. Liefting ◽  
Z. Perez-Egusquiza ◽  
...  
Keyword(s):  
New Zealand ◽  
Plant Virus

Molecular Evolution of the Plant Virus Family Bromoviridae Based on RNA3-Encoded Proteins

2005 ◽  
Vol 61 (5) ◽  
pp. 697-705 ◽  
Author(s):  
Francisco M. Codoñer ◽  
José M. Cuevas ◽  
Jesús A. Sánchez-Navarro ◽  
Vicente Pallás ◽  
Santiago F. Elena
Keyword(s):  
Molecular Evolution ◽  
Plant Virus ◽  
Virus Family ◽  
Encoded Proteins

scholarly journals New plant virus disease records in New Zealand update 20042005

2006 ◽  
Vol 59 ◽  
pp. 374-374
Author(s):  
B.S.M. Lebas ◽  
F.M. Ochoa-Corona ◽  
J.Z. Tang ◽  
D.R. Elliott ◽  
B.J.R. Alexander
Keyword(s):  
New Zealand ◽  
Plant Virus ◽  
Virus Disease ◽  
Plant Virus Disease

Virology Division News: The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation

2004 ◽  
Vol 149 (5) ◽  
pp. 1045-1060 ◽  
Author(s):  
M. J. Adams ◽  
J. F. Antoniw ◽  
M. Bar-Joseph ◽  
A. A. Brunt ◽  
T. Candresse ◽  
...  
Keyword(s):  
Plant Virus ◽  
Species Demarcation ◽  
Virology Division ◽  
Virus Family ◽  
Virology Division News

scholarly journals Widespread Torix Rickettsia in New Zealand amphipods and the use of blocking primers to rescue host COI sequences

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Eunji Park ◽  
Robert Poulin
Keyword(s):  
New Zealand ◽  
Dna Barcoding ◽  
Universal Primers ◽  
Amphipod Species ◽  
Molecular Screening ◽  
Conventional Pcr ◽  
Intracellular Parasites ◽  
Wide Range ◽  
Freshwater Amphipods ◽  
Coi Sequences

Abstract Endosymbionts and intracellular parasites are common in arthropod hosts. As a consequence, (co)amplification of untargeted bacterial sequences has been occasionally reported as a common problem in DNA barcoding. While identifying amphipod species with universal COI primers, we unexpectedly detected rickettsial endosymbionts belonging to the Torix group. To map the distribution and diversity of Rickettsia species among amphipod hosts, we conducted a nationwide molecular screening of seven families of New Zealand freshwater amphipods. In addition to uncovering a diversity of Torix Rickettsia species across multiple amphipod populations from three different families, our research indicates that: (1) detecting Torix Rickettsia with universal primers is not uncommon, (2) obtaining ‘Rickettsia COI sequences’ from many host individuals is highly likely when a population is infected, and (3) obtaining ‘host COI’ may not be possible with a conventional PCR if an individual is infected. Because Rickettsia COI is highly conserved across diverse host taxa, we were able to design blocking primers that can be used in a wide range of host species infected with Torix Rickettsia. We propose the use of blocking primers to circumvent problems caused by unwanted amplification of Rickettsia and to obtain targeted host COI sequences for DNA barcoding, population genetics, and phylogeographic studies.

scholarly journals Pelacakan Gen Sitokrom Oksidase Sub Unit I (COI) DNA Mitokondria Itik Tegal (Anas domesticus) Menggunakan Primer Universal

2013 ◽  
Vol 15 (1) ◽  
pp. 20 ◽  
Author(s):  
Sarwo Edi Wibowo ◽  
Muhammad Anwar Djaelani ◽  
Hermin Pancasakti Kusumaningrum
Keyword(s):  
Mitochondrial Dna ◽  
Native Species ◽  
Egg Production ◽  
Genetic Characterization ◽  
Coi Gene ◽  
Universal Primers ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Gene Data ◽  
Pcr Conditions

Currently local ducks are generally quite difficult to find in a big farm in Inonesia, including Tegal ducks. Tegal ducks is one of the genetic resources native to Indonesia with it’s advantages in terms of high and large egg production. Conservation and development of local ducks have strived to maintain our existence of Indonesian livestock germplasm. If such information is not superior to native species exist, the opportunity to increase his lead further also getting smaller. Tracking the mitochondrial COI gene DNA of Tegal ducks may underlie the process of an organism's genetic characterization. Information about Tegal duck mitochondrial DNA has not been done. The information obtained can be used for optimization of duck products native to Indonesia both in physiological aspects, phylogeny and genetic engineering. The research method used in this research is tracking COI gene data from Gen Bank with the programs Clustal X and Genedoc. Tracking then continued using universal primers HCO and LCO. The results of the data followed up with the isolation and amplification of COI gene mitochondrial DNA as well as the optimization of PCR conditions. The results showed mitochondrial DNA COI gene Tegal ducks were amplified with primer LCO obtain DNA fragments of length less than 250 bp. Kata kunci: duck’s from Tegal, COI gene, mitochondrial DNA

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