scholarly journals Intact parasite-antigen ELISA test: a new dimension for serodiagnosis of Amoebiasis

2018 ◽  
Vol 10 (3) ◽  
pp. 976-980
Author(s):  
Archana Mishra ◽  
Ajay Kumar Sharma ◽  
Vandana Tewari
Keyword(s):  
Clinical Trials ◽  
Entamoeba Histolytica ◽  
Epidemiological Survey ◽  
Elisa Test ◽  
Parasite Antigen ◽  
Antigen Elisa ◽  
Circulating Antibodies

An Intact Parasite Antigen ELISA (IPA-ELISA) has been developed for detection of circulating antibodies against Entamoeba. histolytica. Axenically grown trophozoites of E.histolytica (NIH-200) after glutaraldehyde treatment, are used as Intact Parasite Antigen (IPA) as well biological active and imperishable base. Antigen over the surface of treated cells were allowed to interact with the antibody molecules of the test sera. The techniques of Plate, Dot-and IPA-ELISA are compared of their merits for clinical and epidemiological survey of amoebiasis. IPA-ELISA was found to be more sensitive (96.8%) and specific (92.3%) in detecting circulating antibodies compared to Plate-ELISA (sensitivity 90.5%, specificity 84.6%) and Do-ELISA (sensitivity 92.1%, specificity 86.1%). The entire IPA-ELISA test could be completed within 3 hours. Costwise IPA-ELISA is several times lesser than Dot-and Plate-ELISA. This test is most suitable for field and clinical trials for suspected cases of amoebiasis with no skilled hand required for diagnosis.

scholarly journals Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Abel Villa-Mancera ◽  
Pedro Molina-Mendoza ◽  
Karina Hernández-Guzmán ◽  
Jaime Olivares-Pérez ◽  
Jorge Sarracent-Pérez ◽  
...  
Keyword(s):  
Serological Test ◽  
Sandwich Elisa ◽  
Correlation Coefficients ◽  
Economic Loss ◽  
Elisa Test ◽  
Current Status ◽  
Antigen Elisa ◽  
Faecal Samples ◽  
Diagnostic Sensitivity And Specificity ◽  
Serum Elisa

The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P<0.01) for low seropositivity (r=0.93) and medium seropositivity (r=0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status ofF. hepaticainfection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

scholarly journals PO 8425 THE DIAGNOSTIC AND PROGNOSTIC VALUE OF NEW URINE-BASED LEISHMANIA ANTIGEN DETECTION TESTS

2019 ◽  
Vol 4 (Suppl 3) ◽  
pp. A36.1-A36
Author(s):  
Salah Boshara
Keyword(s):  
Visceral Leishmaniasis ◽  
Rapid Test ◽  
Elisa Test ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Serological Tests ◽  
Kala Azar ◽  
Antigen Elisa ◽  
Protozoan Infection ◽  
Monitoring Treatment

BackgroundVisceral leishmaniasis (VL) also known as kala-azar, is a protozoan infection caused by the L. donovani complex and transmitted by sandflies. Early detection of leishmaniasis is critical in management of patients and for successful control and elimination of the disease. Definitive diagnosis of visceral leishmaniasis is by parasitological demonstration of parasites in splenic, lymph node or bone marrow aspirates, which are collected using invasive methods that are unsuitable in the field. This study aimed to evaluate new less invasive urine-based ELISA and rapid diagnostic test (RDT) assays for diagnosis of VL.MethodsThe newly developed urine ELISA test was evaluated using archived and fresh urine samples collected from parasitologically confirmed VL patients and non-VL cases. Lateral flow assay (LFA) using the ELISA reagents were conducted for day0 samples. Serological tests (DAT, rk28 ICT) were conducted for every patient in the study.ResultsIn 198 patients with suspected VL, urine rapid test had a sensitivity of 72.2% and exhibited a specificity of 93.42%. Leishmania antigen ELISA had a sensitivity of 83.33% and a specificity of 95.05%. All VL-confirmed cases were followed up during the treatment period, the Leishmania antigen ELISA became negative 2 months after completion of treatment in most patients.ConclusionUrine lateral flow assay is a simple addition to the diagnostics of VL particularly at field level and as a complementary test for the diagnosis of VL in smear-negative cases. Further enhancement of the test will define its performance in monitoring treatment. Further studies are recommended to evaluate the performance of both tests in the diagnosis of HIV-co-infected cases.

scholarly journals Seroprevalence and risk factors associated to Neospora caninum in dairy cattle herds in the municipality of Pasto, Colombia

2013 ◽  
pp. 3311-3316 ◽  
Author(s):  
Darío Cedeño Q ◽  
Bibiana Benavides B
Keyword(s):  
Risk Factors ◽  
Dairy Cattle ◽  
Neospora Caninum ◽  
Binary Logistic Regression ◽  
Epidemiological Survey ◽  
Elisa Test ◽  
Effective Control ◽  
Serum Samples ◽  
Factors Associated ◽  
Cattle Herds

ABSTRACTObjective. To determine seroprevalence and risk factors associated to Neospora caninum in non-vaccinated dairy cattle against infectious agents of reproductive syndrome in the municipality of Pasto, Colombia. Materials and methods. Farms over 2527 meters over sea level were selected, a total of 238 serum samples of Holstein cows were collected and analyzed using the indirect ELISA test to determine N. caninum seropositivity. An epidemiological survey was realized in each herd which included variables related to health and management measures of cattle. A multivariate analysis of binary logistic regression was used with a confidence interval of 95% (p<0.05) using SPSS19® program. Results. The estimated prevalence of N. caninum was 76.9%. The risk factors associated to neosporosis infection in the analyzed farms are as follows: residues of abortions generally left outdoors and not buried (OR 3.81, 95% CI 1.5 - 9.6); dogs fed with leftovers (OR 15.44 IC 95% 1.94-123.22) and bulls allowed to mate with cows (OR 19.68, 95% CI 2.34 - 165.52). Conclusions. The high prevalence of N. caninum and the low abortion rate in dairy herds of the municipality of Pasto corroborated no existence of the disease in all animals serologically positive, but it did suggest that at some point in their lives they were exposed to N. caninum. From the identified risk factors in this study, recommendations can be provided for an effective control of reproductive diseases like Neosporosis present in this region.

scholarly journals Detection of SARS-CoV-2 by antigen ELISA test is highly swayed by viral load and sample storage condition

2021 ◽  
Author(s):  
Nihad Adnan ◽  
Shahad Saif Khandker ◽  
Ahsanul Haq ◽  
Mousumi Akter Chaity ◽  
Abdul Khalek ◽  
...  
Keyword(s):  
Viral Load ◽  
Storage Condition ◽  
Elisa Test ◽  
Sample Storage ◽  
Antigen Elisa

scholarly journals Epidemiological Survey on Porcine Cysticercosis in Nay Pyi Taw Area, Myanmar

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Tin Aye Khaing ◽  
Saw Bawm ◽  
Soe Soe Wai ◽  
Ye Htut ◽  
Lat Lat Htun
Keyword(s):  
Risk Factors ◽  
Taenia Solium ◽  
Epidemiological Survey ◽  
Elisa Test ◽  
Meat Inspection ◽  
Cross Sectional ◽  
Porcine Cysticercosis ◽  
Husbandry System ◽  
Questionnaire Surveys ◽  
Associated Risk Factors

Cross-sectional surveys were conducted to determine the prevalence and associated risk factors of Taenia solium cysticercosis in pigs within Nay Pyi Taw area, Myanmar. Meat inspection in three slaughterhouses, ELISA test, and questionnaire surveys were conducted in this study. Three hundred pigs were inspected in slaughterhouses and 364 pigs were randomly selected and examined from 203 households from three townships in Nay Pyi Taw area. The prevalence of porcine cysticercosis in meat inspection was 23.67% (71/300). Seroprevalence of T. solium cysticercosis in pigs in the study area was 15.93% (58/364). Significant associated risk factors with T. solium cysticercosis were gender (OR=3.0; 95% CI=1.7–5.4), increased age (OR=2.3; 95% CI=1.2–4.2), husbandry system (OR=5.1; 95% CI=2.4–11.2), feed type (OR=16.9; 95% CI=2.3–124.3), not using anthelmintics in pigs (OR=11.9; 95% CI=5.0–28.5), not using anthelmintics in owner (OR=2.5; 95% CI=1.4–4.4), no hand-washing before feeding (OR=31.5; 95% CI=4.3–230.9), and pork consumption of owner (OR=37.4; 95% CI=9.0–156.1) in the study area. This is the first report of porcine cysticercosis in Myanmar.

scholarly journals Sero-prevalence and Risk Factors for Hepatitis A Virus Infection among Pregnant Women at the Samandin Medical Center, Ouagadougou, Burkina Faso

2019 ◽  
pp. 1-9
Author(s):  
Rouamba Poda Somda Hortense ◽  
Ouoba Jean Bienvenue ◽  
Traoré Kuan Abdoulaye ◽  
Ouoba Bruno Lalidia ◽  
Kagambéga Asséta ◽  
...  
Keyword(s):  
Risk Factors ◽  
Burkina Faso ◽  
Pregnant Women ◽  
Hepatitis A Virus ◽  
Medical Center ◽  
Point Of Care ◽  
Epidemiological Survey ◽  
Elisa Test ◽  
Cross Sectional ◽  
Point Of Care Test

Aims: This study determined prevalence and possible risk factors associated with HAV infection and compared Immunochromatography (IgG) rapid point of care test with standard ELISA test for diagnosis. Study Design:  Cross sectional epidemiological survey. Place and duration of Study: The study was carried out at the Samandin Medical Center (Ouagadougou, Burkina Faso), from July 2017 to December 2017. Methodology: A total of 180 pregnant women were recruited at the Samandin Medical Center. Anti-HAV IgM and IgG detection tests were performed using AccuDiag™ HAV ELISA kit (Calabasas, CA, USA) and SD BIOLINE HAV IgG/IgM Rapid Diagnostic Test (Gyeonggi-do, Korea). Results: IgG anti-HAV antibodies (AccuDiag Elisa) were found with a prevalence of 88.9% (160/180) and Anti-HAV IgM were 1.1% (2/180). Of the 160 AccuDiag HAV IgG positive samples, 44.4% (80/180) tested positive using SD BIOLINE Diagnostics HAV IgG. Regarding risk factors, 86.9% (86/99) of the IgG positive women were illiterate; 90.7% (39/43) lived mostly in peripheral areas; 89.2% (74/83) were household workers and 88.6% (93/105) used fountain water. Conclusion: Our results demonstrated poor agreement between the assays tested, which are consistent with previous reports demonstrating significant variability between HAV ELISA and RDT HAV. Moreover, the detection of HAV specific IgM antibodies in two asymptomatic pregnant women signaled the current circulation of HAV in this area.

scholarly journals Epidemiological Survey of Rotaviruses Responsible for Infantile Diarrhea by the Immunomolecular Technique in Cotonou (Benin, West Africa)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Jijoho Mischaël Michel Agbla ◽  
Annick Capo-Chichi ◽  
Alidéhou Jerrold Agbankpé ◽  
Tamègnon Victorien Dougnon ◽  
Anges William M. Yadouleton ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Epidemiological Data ◽  
Epidemiological Survey ◽  
Elisa Test ◽  
University Hospital ◽  
Laboratory Manual ◽  
Infantile Diarrhea ◽  
Stool Samples ◽  
Rotavirus Diarrhea ◽  
Epidemiological Characteristics

Rotavirus remains the main causative agent of gastroenteritis in young children, in countries that have not yet introduced the vaccine. Benin, in order to implement the WHO recommendations, projects to introduce the rotavirus vaccine in 2018 as part of its Expanded Program on Immunization. But before the introduction of this vaccine, epidemiological data on rotavirus infections and rotavirus genotypes circulating in Benin should be available. The aim of this study is to generate epidemiological data on infantile rotavirus diarrhea in Benin. In order to determine the epidemiological characteristics and electrophoretypes of rotavirus responsible for gastroenteritis in diarrheic children aged 0 to 5 years, 186 stool samples were collected according to the WHO Rotavirus Laboratory Manual from March 2014 to February 2015 at Suru-Lere University Hospital Center. Detection of rotavirus antigen was performed by the ELISA test, followed by molecular characterization using polyacrylamide gel electrophoresis. 186 stool samples were analyzed for rotavirus, and seventy-three (39.2%) were found to be positive for rotavirus antigen by ELISA. Children aged 3 to 24 months were the most affected by rotavirus diarrhea in this study. Of the seventy-three children affected with rotavirus diarrhea, 27 (37%) had vomiting accompanied by dehydration and fever. Results based on electrophoresis showed that, among the 73 samples tested, 38 yielded typical rotavirus electrophoretic migration profiles.

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