CHROMOSOME DAMAGE INDUCED BY ARTIFICIAL SEED AGING IN BARLEY. I. GERMINABILITY AND FREQUENCY OF ABERRANT ANAPHASES AT FIRST MITOSIS

1981 ◽  
Vol 23 (2) ◽  
pp. 267-280 ◽  
Author(s):  
Minoru Murata ◽  
Eric E. Roos ◽  
Takumi Tsuchiya
Keyword(s):  
Moisture Content ◽  
Seed Storage ◽  
Germination Percentage ◽  
Mitotic Division ◽  
Root Tips ◽  
Hordeum Vulgare L ◽  
Seed Moisture Content ◽  
Seed Aging ◽  
Seed Moisture ◽  
Higher Temperature

In order to study the genetic changes which occur during seed storage, barley (Hordeum vulgare L. 'Himalaya') seeds were subjected to artificial aging using six combinations of temperature (21 °C, 32 °C, and 38 °C) and seed moisture content (12% and 18%). With increasing time in storage, germination of the seeds was delayed and reduced. Abnormal seedlings without roots also occurred with increased storage. Higher temperature and seed moisture content induced rapid loss of germinability. At the first mitotic division in the root tips, the frequency of aberrant anaphases and of roots with aberrations increased with increased storage time. The frequencies of aberrant anaphases and of roots with aberrations were also increased by higher temperature and seed moisture content. Frequencies of aberrant anaphases and of roots with aberrations were negatively correlated with germination percentage. This indicated that the frequency of chromosomal aberrations induced by seed aging might be estimated from the germination percentages.

scholarly journals Drying and physiological quality of palheteira seeds (Clitoria fairchildiana R.A. Howard)

2020 ◽  
Vol 9 (9) ◽  
pp. e858998157
Author(s):  
Érica Coutinho David ◽  
Bressa Karolina Dias Cardoso ◽  
Josiene Amanda dos Santos Viana ◽  
Eniel David Cruz
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Seed Storage ◽  
Germination Percentage ◽  
Hot Water ◽  
Degraded Land ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Physiological Quality

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.

scholarly journals Germination of Nonstratified Japanese Tree Lilac Seeds as Influenced by Seed Capsule Maturity and Moisture Content

2014 ◽  
Vol 24 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Todd P. West ◽  
Samuel L. DeMarais ◽  
Chiwon W. Lee
Keyword(s):  
Moisture Content ◽  
Germination Percentage ◽  
Seedling Production ◽  
Fresh Weight ◽  
Seed Moisture Content ◽  
Seed Collection ◽  
The North ◽  
Seed Moisture ◽  
Embryo Dormancy ◽  
Seed Stratification

Temperate-zone woody plant species generally require seed stratification to overcome embryo dormancy. Embryo dormancy is variable in japanese tree lilac (Syringa reticulata) with a recommendation of 30- to 90-days stratification at 1–5 °C. Cultivar propagation generally is done by grafting onto seedling rootstocks. It would be advantageous to rootstock seedling production to be able to reduce or eliminate the need for seed stratification to increase germination numbers as well as having production moved from field beds to greenhouses for quicker production of high-quality rootstock plants suitable for grafting. Research objective of this study was to determine if “green” seed could be used without the need of stratification for japanese tree lilac seedling production. Seed capsule fresh weight and seed moisture content were evaluated to determine if these factors could be used as predictors of germination percentages. Seed was randomly collected at the North Dakota State University campus in Fargo, ND, for seven consecutive weeks starting in Sept. 2011 and 2012. Germination and seed moisture tests were performed weekly. Germination percentage was highest (89.5%) at week 2 and steadily decreased to 0% at week 7. Germination percentages were 77.5%, 89.5%, 78.5%, 67%, 24.5%, 1.5%, and 0% for consecutive collection weeks 1–7, respectively. Seed moisture content was 59.0%, 52.6%, 49.8%, 51.8%, 44.5%, 27.4%, and 8.6% for collection weeks 1–7, respectively. Germination percentage was directly correlated with seed moisture content and decreased as seed capsules matured (natural drying and splitting of capsule seem to disperse seed) during the fall season. Data suggest that timing of fall seed collection from japanese tree lilac is critical and must be done before maturation of the seed capsule to avoid the stratification requirement. Seed capsules with an average fresh weight higher than 0.2 g and seed moisture content greater than 50% produced the highest germination rates without requiring stratification.

scholarly journals Effect of different storage materials on the seed temperature, seed moisture content and germination of wheat under farmer’s field condition of Kailali district, Nepal

2019 ◽  
Vol 11 (4) ◽  
pp. 352-355
Author(s):  
N. Khatri ◽  
D. Pokhrel ◽  
B.P. Pandey ◽  
K.R. Pant ◽  
M. Bista
Keyword(s):  
Moisture Content ◽  
Block Design ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Seed Moisture Content ◽  
Quality Performance ◽  
Storage Materials ◽  
Seed Moisture ◽  
Randomized Complete Block Design ◽  
Long Time

Abstract. A study was conducted at four Village Development Committees (Masuriya, Udashipur, Basauti and Pahalmanpur) of Kailali dis- trict, Far western Region, Nepal in 2016/17 to find out the effect of different storage materials on the seed moisture content and germination percentage of wheat. Seeds of wheat were packed using five storage materials (metal seed bin, earthen pot, purdue improved crop storage / Pics/ bag, gunny bag and super grain bag). The stored seed samples were drawn at three-month intervals starting from May 2016 to January 2017. The experiment was arranged in Randomized Complete Block Design (RCBD) in four replications. The results of analysis of variance revealed that the effect of storage materials was significant (P≤0.05) on seed moisture content and seed germination of wheat. After nine months of storage, the seeds stored in Pics bag recorded the lowest moisture (13.3%) and temperature (26.4°C), and the highest germination percentage (91.75%). The lowest quality performance (high moisture and temperature, low germination) of stored seeds was observed in an earthen pot. From the results obtained, it was concluded that wheat seed could be stored for a long time in Pics bag without deteriorating its quality parameters.

scholarly journals Effect of different storage conditions and seed treatments on seed viability in soybean [Glycine max (L.) Merr.]

2017 ◽  
Vol 9 (1) ◽  
pp. 245-252
Author(s):  
J. B. Patel ◽  
Jyoti Sondarva ◽  
C. A. Babariya ◽  
R. R. Rathod ◽  
V. J. Bhatiya
Keyword(s):  
Moisture Content ◽  
Cold Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Seed Treatments ◽  
Seed Moisture Content ◽  
Seed Vigour ◽  
Seed Moisture ◽  
Vigour Index ◽  
Leaf Powder

The present investigation was carried out in laboratory of the Department of Seed Science and Technolo-gy, College of Agriculture, Junagadh Agricultural University, Junagadh from the April 2013 to April 2015, wherein two kg of freshly harvested quality seed of soybean cv. Gujarat Junagadh Soybean 3 having high germination percentage and low moisture content (below 8%) was taken for each repetition and for each combination of treat-ments. The treatment consisted of two storage conditions (C) viz., C1 (Ambient temperature) and C2 (Cold storage at 7oC + 2oC), and five seed treatments (S) viz., S1 = Control, S2 = Carbendazim @ 2g/kg seed, S3 = Mancozeb @ 2g/ kg seed, S4 = Neem leaf powder @ 10g/kg seed, and S5 = Neem Oil @ 5 ml/kg seed. The experiment was carried out using Completely Randomized Design (Factorial) repeated three times. After proper mixing or smearing the seeds as per the treatments, seeds were packed in cloth bag and kept in laboratory under two different storage conditions. Observations were recorded at 90 days interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results revealed that storage condition (C) and seed treatments (S) exhibited significant differences almost for the all the traits for germination and seedling parameters after 2 years of storage. The results of soybean seed stored in two different storage conditions showed that on an average, the seed stored under cold storage (70C + 20C) noted higher values for all the traits studied except seed moisture content after 2 years of storage. Among the seed treatments, on an average, after 2 years of seed storage, significantly (P<0.05) higher values were recorded by all the seed treatments over the control. However, seed treated with Mancozeb @ 2g/kg of seed recorded the significantly highest germination percentage (71.50 %) and it was at par with Neem leaf powder @ 10 g / kg seed (70.67%) and Carbendazim @ 2g /kg seed (69.67%) after 2 years of storage. The germination percentage noted in control treatment was 33.17 per cent after 2 years of storage. An ISTA standard for germination in soybean is 70 per cent. Most of the interactions effects were found significant (P<0.05) for all the traits studied.

scholarly journals Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka

2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Initial Moisture Content ◽  
Seed Viability ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Input Model ◽  
Seed Storability

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>

scholarly journals Evaluation of Temperature and Moisture Content during Storage on the Germination of Flowering Annual Seed

HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1003-1006 ◽  
Author(s):  
William J. Carpenter ◽  
Eric R. Ostmark ◽  
John A. Cornell
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Storage Temperatures

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.

scholarly journals Conservation of Garcinia imberti Bourd. through seeds

2019 ◽  
Vol 6 (2) ◽  
pp. 243-251
Author(s):  
M Anto ◽  
M Angala ◽  
P S Jothish ◽  
C Anilkumar
Keyword(s):  
Seed Germination ◽  
Relative Humidity ◽  
Moisture Content ◽  
Gibberellic Acid ◽  
Biosphere Reserve ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Evergreen Forests ◽  
Seed Moisture ◽  
Subsequent Exposure

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.

scholarly journals Engineered Jute Bags for Storing Food Grains within Harrington’s Safe Zone of Seed Moisture Content

2021 ◽  
Author(s):  
Kennedy Odokonyero ◽  
Himanshu Mishra ◽  
Adair Gallo Junior
Keyword(s):  
Moisture Content ◽  
Low Cost ◽  
Seed Storage ◽  
Water Repellent ◽  
Seed Moisture Content ◽  
Post Harvest ◽  
Food Grains ◽  
Seed Moisture ◽  
Jute Fabrics ◽  
Jute Bags

<p>Harrington’s thumb rule states that for every 1% increase in seed moisture content (SMC), seed lifetime decreases by 50%. Thus, to avoid post-harvest grain losses, stored seeds must be insulated from water. Although the jute bags typically used to store grains afford an ecofriendly, durable, and low-cost storage solution, their hydrophilic nature makes them vulnerable to wetting from airborne humidity and precipitation events. To address this issue, we treated jute fabrics with alkali and wax to render them water-repellent. A 2-month seed storage experiment revealed that wax-coated jute bags (WCJBs) outperformed control jute bags (CJBs) at safeguarding wheat (<i>Triticum aestivum</i>) grains exposed to 55%–98% relative humidity. Specifically, grains stored in WCJBs exhibited 15%–50% less SMC than grains stored in CJBs, which led to a 21%–66% enhancement in the germination efficacy of WCJB-stored seeds. This engineering solution could enhance the potential of jute bags to reduce post-harvest losses.</p>

scholarly journals RESPONSE OF DIFFERENT STORAGE CONTAINERS IN RICE GENOTYPES DURING STORAGE

2021 ◽  
pp. 21-35
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Aluminum Foil ◽  
Germination Percentage ◽  
Seed Moisture Content ◽  
Storage Container ◽  
Seed Moisture ◽  
Vigor Index ◽  
Rice Genotypes ◽  
Good Quality Seed

Rice is the staple food of more than half the world’s population and is being cultivated in Asia since ancient time’s generation after generation. Good quality seeds are the seeds of the green revolution, it has been assertively and emphatically shown that 15-20% increased yield could be achieved by the use of good quality seeds alone. Good quality seed is one of the most important requirements for production and supply to achieve higher production and productivity in any crop. The seed quality is depended on various factors Viz. genetic, edaphic, environmental, biotic, etc. Good quality seed production depends on complex conditions evoking the most favorable interactions between the genetic makeup of the seed and the environment, under which it is produced, harvested, processed, and stored. Seeds undergo deterioration at various levels during storage resulting in a decline in vigor and viability. With the above considerations, the present investigation has been undertaken to observe the potentials of ten rice genotypes and observed its seed quality after harvesting in 2016-2017 at Seed Testing Laboratory, Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India. Just after harvesting G2 (Gobindabhog) and G9 (IR-36) showed highest germination percentage (97.66) and G9 (IR-36) showed highest Vigor index (3204.13). But most of the varieties germination and vigor were nearer to G2 & G9. Among the containers with seed moisture content aluminum foil packet with seed moisture content, 8% is the best storage container for rice among the other containers as up to one-year storage vigor index was observed highest in that conditions.

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