scholarly journals Impact of honey-enriched mulberry diet on the energy metabolism of the silkworm, Bombyx mori

2020 ◽  
Vol 12 (2) ◽  
pp. 133-145
Author(s):  
S. Siva Prasad ◽  
R. Madhavi
Keyword(s):  
Energy Metabolism ◽  
Oxidative Phosphorylation ◽  
Bombyx Mori ◽  
Larval Stage ◽  
Fat Body ◽  
Activity Levels ◽  
Energy Reserves ◽  
Related Energy ◽  
Energy Needs ◽  
Utilization Patterns

The present study was taken-up with a view to clearly define the role of oxidative phosphorylation vis-a-vis transdeamination in Bombyx mori metamorphosis, under the influence of honey-enriched mulberry diet. Therefore, the study examined the accumulation and utilization patterns of carbohydrate (glycogen, trehalose, glucose) and non-carbohydrate energy reserves (proteins, amino acids) in its fat body during larval, pupal and adult stages. In accordance with Hutchinson’s investment principle, the energy reserves invested during larval stage are partly used in pupal stage and those invested during larval and pupal stages are used in adult stage. Their utilization patterns are correlated with the activity levels of succinate (SDH) and glutamate (GDH) dehydrogenases and aspartate (AAT) and alanine (ALAT) aminotransferases and changes thereof were interpreted in terms of glycolytic oxidative phosphorylation and non-glycolytic transdeamination.  The trends in mass incorporation rates vis-à-vis enzyme activities indicated that the metabolism-related energy needs of all metamorphic events are majorly met through a gluconeogenetic mechanism called transdeamination, while the behavioural-related energy demands of larval and pupal stages are fulfilled through glycolytic-based oxidative phosphorylation. The activity trends further indicated that AAT plays major role in meeting the energy needs of larva and pupa, while GDH predominantly meets the energy requirements of reproduction in adults. The honey-enriched mulberry diet showed stage-specific and pathway-specific impacts on energy metabolism. It positively reinforced the energy metabolism in larval stage, but showed no significant effect in pupal and adult stages. Similarly, it showed more promising effect on glycolytic-oxidative phosphorylation and null or neutral effect on transdeamination.

scholarly journals Effect of SSP – 11 on Weight and Tissue Composition of 4th Instar Larvae of Silkworm, Bombyx mori

2017 ◽  
Vol 11 ◽  
pp. 11
Author(s):  
K. Υenkata Rami Reddy ◽  
K. Sashindran Nair ◽  
S. Β. Magadum ◽  
R. Κ. Datta
Keyword(s):  
Bombyx Mori ◽  
Artificial Diet ◽  
Fat Body ◽  
Activity Levels ◽  
Tissue Composition ◽  
Fresh Weight ◽  
Total Proteins ◽  
Larval Body ◽  
Total Carbohydrates ◽  
Silkworm Bombyx Mori

The anti-juvenoid, SSP-11 ((E) 4-chloro-a, a, a-trifluro-N [ 1 (1 H-imidazole- 1 –y1)- 2-propoxy ethylyledene] O, toludine) was used to induce trimoulters from tetramoulter larvae of the silkworm, Bombyx mori (race NB7). The compound was fed for two days through an artificial diet at the concentration of 200 ppm to newly ecdysed 4th stadium larvae. Body and silkgland fresh weight were recorded daily in the 4th larval stadium and protein profiles of haemolymph, fat body and silkgland were determined at the end of the stadium. In larvae treated with SSP-11, feeding period in stadium 4 was prolonged by 3-4 days and 80-85% of the larvae started to spin as trimoulters. Eight and thirty fold increases in the larval body and silkgland weights respectively were the result of SSP-11. Further, total proteins, total carbohydrates, glycogen and nucleic acid (DNA & RNA) also increased. The activity levels of alanine aminotransferase and aspartate aminotransferasc increased significantly than in the control indicating increased mobilization of aminoacids into transamination activities.

scholarly journals Transcriptome analysis reveals potential function of long non-coding RNAs in 20-hydroxyecdysone regulated autophagy in Bombyx mori

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Huili Qiao ◽  
Jingya Wang ◽  
Yuanzhuo Wang ◽  
Juanjuan Yang ◽  
Bofan Wei ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Target Gene ◽  
Fat Body ◽  
Expression Profiles ◽  
Functional Characterization ◽  
Differentially Expressed ◽  
Post Injection ◽  
Biological Processes ◽  
Potential Target ◽  
Non Coding Rnas

Abstract Background 20-hydroxyecdysone (20E) plays important roles in insect molting and metamorphosis. 20E-induced autophagy has been detected during the larval–pupal transition in different insects. In Bombyx mori, autophagy is induced by 20E in the larval fat body. Long non-coding RNAs (lncRNAs) function in various biological processes in many organisms, including insects. Many lncRNAs have been reported to be potential for autophagy occurrence in mammals, but it has not been investigated in insects. Results RNA libraries from the fat body of B. mori dissected at 2 and 6 h post-injection with 20E were constructed and sequenced, and comprehensive analysis of lncRNAs and mRNAs was performed. A total of 1035 lncRNAs were identified, including 905 lincRNAs and 130 antisense lncRNAs. Compared with mRNAs, lncRNAs had longer transcript length and fewer exons. 132 lncRNAs were found differentially expressed at 2 h post injection, compared with 64 lncRNAs at 6 h post injection. Thirty differentially expressed lncRNAs were common at 2 and 6 h post-injection, and were hypothesized to be associated with the 20E response. Target gene analysis predicted 6493 lncRNA-mRNA cis pairs and 42,797 lncRNA-mRNA trans pairs. The expression profiles of LNC_000560 were highly consistent with its potential target genes, Atg4B, and RNAi of LNC_000560 significantly decreased the expression of LNC_000560 and Atg4B. These results indicated that LNC_000560 was potentially involved in the 20E-induced autophagy of the fat body by regulating Atg4B. Conclusions This study provides the genome-wide identification and functional characterization of lncRNAs associated with 20E-induced autophagy in the fat body of B. mori. LNC_000560 and its potential target gene were identified to be related to 20-regulated autophagy in B. mori. These results will be helpful for further studying the regulatory mechanisms of lncRNAs in autophagy and other biological processes in this insect model.

scholarly journals Eicosanoids mediate induction of immune genes in the fat body of the silkworm, Bombyx mori

FEBS Letters ◽  
1997 ◽  
Vol 419 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Isao Morishima ◽  
Yoshiaki Yamano ◽  
Kenji Inoue ◽  
Noriyuki Matsuo
Keyword(s):  
Bombyx Mori ◽  
Fat Body ◽  
Immune Genes ◽  
Silkworm Bombyx Mori

Antiapoptotic activity of 30 kDa lipoproteins family from fat body tissue of silkworm,Bombyx mori

2014 ◽  
Vol 22 (5) ◽  
pp. 629-638 ◽  
Author(s):  
Britto Cathrin Pakkianathan ◽  
Nitin Kumar Singh ◽  
Simone König ◽  
Muthukalingan Krishnan
Keyword(s):  
Bombyx Mori ◽  
Fat Body ◽  
Body Tissue ◽  
Antiapoptotic Activity ◽  
Silkworm Bombyx Mori

scholarly journals Oxidative Phosphorylation: A Target for Novel Therapeutic Strategies Against Ovarian Cancer

Cancers ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 337 ◽  
Author(s):  
Amruta Nayak ◽  
Arvinder Kapur ◽  
Lisa Barroilhet ◽  
Manish Patankar
Keyword(s):  
Ovarian Cancer ◽  
Oxidative Phosphorylation ◽  
Aerobic Glycolysis ◽  
Ovarian Tumors ◽  
Metabolic Adaptation ◽  
Ovarian Cancer Stem Cells ◽  
Critical Control Points ◽  
Energy Needs ◽  
Oxidative Phosphorylation Pathway ◽  
Novel Therapeutic Strategies

Aerobic glycolysis is an important metabolic adaptation of cancer cells. There is growing evidence that oxidative phosphorylation is also an active metabolic pathway in many tumors, including in high grade serous ovarian cancer. Metastasized ovarian tumors use fatty acids for their energy needs. There is also evidence of ovarian cancer stem cells privileging oxidative phosphorylation (OXPHOS) for their metabolic needs. Metformin and thiazolidinediones such as rosiglitazone restrict tumor growth by inhibiting specific steps in the mitochondrial electron transport chain. These observations suggest that strategies to interfere with oxidative phosphorylation should be considered for the treatment of ovarian tumors. Here, we review the literature that supports this hypothesis and describe potential agents and critical control points in the oxidative phosphorylation pathway that can be targeted using small molecule agents. In this review, we also discuss potential barriers that can reduce the efficacy of the inhibitors of oxidative phosphorylation.

Clathrin‐dependent endocytosis predominantly mediates protein absorption by fat body from the hemolymph in Bombyx mori

2019 ◽  
Vol 27 (4) ◽  
pp. 675-686
Author(s):  
Yu‐Jie Wang ◽  
Shu‐Yan Li ◽  
Jia‐Ye Zhao ◽  
Kang Li ◽  
Jing Xu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Fat Body ◽  
Protein Absorption
Sign in / Sign up

Export Citation Format

Share Document