scholarly journals MICRO PROPAGATION OF LILIUM CANDIDUM UNDER 2,4- DICHLOROPHENOXYACETIC ACID AND 6-BENZYLAMINOPURINE GROWTH REGULATORS

2015 ◽  
Vol 44 (1) ◽  
pp. 123-132
Author(s):  
J. Zakrzewski

Growth response of <i>Pinus silvestris</i> hypocotyl sections to some synthetic growth regulators and related substances was studied. Elongation of hypocotyl sections was stimulated by naphtaleneacetic acid, indole-3-acetic acid, in-dole-3-propionic acid, indole-3-butyric acid, 2,4-dichlorophenoxyacetic acid, indoleaoetic amide, indoleacetic nitrile and coumarin. Indole-3-acetic acid and naphtaleneacetic acid extended period of growth up to 16 and 24 hours, respectively. Growth was inhibited by kinetin, trans-cinnamic acid and 2,3,5-tri-iodobenzoic acid. No effect of gibberellic acid, tryptophan and biotin was observed.


2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Yaser Mollapur ◽  
Seied Mehdi Miri ◽  
Ebrahim Hadavi

AbstractAn investigation was carried out to determine the ability of foliar fertilizers and growth regulators to prevent pre-harvest drop and enhance navel orange fruit quality. Fifteen year old trees of the Thompson Navel orange variety were sprayed with aqueous solutions of one of the following: methanol (0.13%), calcium nitrate (0.25%), zinc sulfate (1%) + urea (0.5%), fermented sugar- cane extract (2.8%), salicylic acid (1 or 3 mM), citric acid (5 mM), or 2,4-dichlorophenoxyacetic acid (2,4-D) (0.002%). Results indicated that foliar treatment with 2,4-D reduced fruit drop (2.6%) as compared to the control (15.2%). Fruit decay was delayed and flavor improved by 5 mM citric acid. Weight loss was reduced after 3mM salicylic acid applica- tion, while methanol (0.13%), salicylic acid (1 and 3 mM) and citric acid (5 mM) caused a delay in fruit coloring.


2016 ◽  
Vol 68 (2) ◽  
pp. 399-404 ◽  
Author(s):  
Milan Dragicevic ◽  
Ana Simonovic ◽  
Milica Bogdanovic ◽  
Angelina Subotic ◽  
Nabil Ghalawenji ◽  
...  

Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 - GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.


2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119


1984 ◽  
Vol 102 (2) ◽  
pp. 437-442 ◽  
Author(s):  
Lynda D. Wickham ◽  
H. C. Passam ◽  
L. A. Wilson

SummaryWhen pre-harvest foliar applications of growth regulators were made to vines of Dioscorea esculenta cv. Chinese Yam and D. alata cv. White Lisbon there were no obvious effects on tubers of D. alata, but dramatic effects on tuber development, storage and germination were obtained with D. esculenta tubers. Gibberellic acid caused marked extensions in tuber dormancy, 2,4-dichlorophenoxyacetic acid resulted in marked callus development, delayed germination and abnormal shoot and root development and indole acetic acid resulted in fingering of the tubers. It was concluded that there was potential for commercial exploitation of pre-harvest gibberellic acid treatments in extension of dormancy in Chinese Yam tubers and that the lack of response of D. alata tubers to foliar application of growth regulators warranted further investigation.


1974 ◽  
Vol 82 (2) ◽  
pp. 245-249 ◽  
Author(s):  
M. Abdel-Rahman ◽  
F. M. R. Isenberg

SummaryPreharvest sprays with 6-hydroxy-3-(2H)-pyridazinone (MH), or immersion in solutions of MH, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-chloroethylphosphonic acid (ethephon), coumarin or (2-chloroethyl) trimethylammonium chloride (CCC) inhibited both sprouting and rooting of treated carrots during storage at 0 °C whencompared with water-dipped or untreated carrots, while N-6-benzyl-adenine (BA) dips stimulated extensive rooting and sprouting. In contrast, immersion in gibberellic acid (GA3) solutions had very little effect.Storage atmospheres containing 1, 2.5, 5 or 10% oxygen inhibited both sprouting and rooting but increased mould infection. Atmospheres containing 21% (air) or 40% oxygen reduced mould infection, but increased sprouting and rooting of stored carrots. Washing carrots in water before storage did not significantly affect mould infection, while washing in 0.5% clorox increased it. However, washing in water followed by immersion in MH inhibited both sprouting and rooting and also reduced mould infection. ‘Jumbo’ and large-size carrots stored better than medium and small carrots.


2016 ◽  
Vol 19 (4) ◽  
pp. 105-116
Author(s):  
Thang Thanh Tran ◽  
Huong Thanh Tran

In this study, plant growth regulators included 2,4-dichlorophenoxyacetic acid (2,4-D), picloram, 6-benzylaminopurine (BA) and thidiazuron (TDZ), at different concentrations were used individually or in combination to induce adventitious shoots from bulb scales of Lily Sorbonne. Morphological and physiological changes in shoot formation from bulb scales were analysed. The maximum number of shoots per explant were obtained on Murashige and Skoog medium (MS) supplemented with 2,4-D 1 mg/L, BA 1,5 mg/L, zeatin 0,2 mg/L and indole-3-acetic acid (IAA) 0,5 mg/L. The adventitious shoots were derived from parenchymal cells, which placed under epidermis cells. This process included the following stages: activation of cell division with large nucleus, thin-walled and without starch granules; initiating of meristematic region; formation of shoot primordium and shoot with leaves. Use of 1-naphthylphthalamic acid (1-NOA) and N-1-naphthoxyacetic acid (NPA), auxin transport inhibitors, showed the role of polar auxin transport in shoot formation. The correlation of plant hormone, respiration rate and shoot formation from bulb scales was discussed.


2006 ◽  
Vol 6 ◽  
pp. 169-175
Author(s):  
A.E. De Silva ◽  
M.A. Kadir ◽  
M.A. Aziz ◽  
S. Kadzimin

Differential effect of plant growth regulators and additives in proliferation of 18-month-old calli ofAnanas comosusL. cv. Moris were assessedin vitro. The proliferation of callus relied on the growth regulators and additives. Of the different auxins supplemented in the Murashige and Skoog (MS) media, 32.22 μM α-naphthaleneacetic acid (NAA) gave the highest mean fresh weight of callus (46.817 g). Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was inferior to NAA, while b-naphthoxy acetic acid (BNOA) and p-chlorophenoxy acetic acid (4-CPA) were not effective in proliferating 18-months old callus. Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus. However, in terms of callus production, NAA at 32.22 μM was economically better.


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