Investigation of cancer cell lines for peptide receptor-targeted drug development

Aim: Phytocompounds are important due to their uniqueness, however, only few reach the development phase due to their poor pharmacokinetics. Therefore, preassessing the absorption, distribution, metabolism, excretion and toxicity (ADMET) properties is essential in drug discovery. Methodology: Biologically diverse databases (Phytochemica, SerpentinaDB, SANCDB and NuBBEDB) covering the region of India, Brazil and South Africa were considered to predict the ADMET using chemoinformatic tools (Qikprop, pkCSM and DataWarrior). Results: Screening through each of pharmacokinetic criteria resulted in identification of 24 compounds that adhere to all the ADMET properties. Furthermore, assessment revealed that five have potent anticancer biological activity against cancer cell lines. Conclusion: We have established an open-access database (ADMET-BIS) to enable identification of promising molecules that follow ADMET properties and can be considered for drug development.

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Comparing cellular uptake and cytotoxicity of targeted drug carriers in cancer cell lines with different drug resistance mechanisms

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2010.11.004 ◽

2011 ◽

Vol 7 (3) ◽

pp. 324-332 ◽

Cited By ~ 46

Author(s):

Tingjun Lei ◽

Supriya Srinivasan ◽

Yuan Tang ◽

Romila Manchanda ◽

Abhignyan Nagesetti ◽

...

Keyword(s):

Drug Resistance ◽

Cell Lines ◽

Cancer Cell ◽

Cellular Uptake ◽

Drug Carriers ◽

Cancer Cell Lines ◽

Resistance Mechanisms ◽

Targeted Drug

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Abstract 1918: InnoPanelTM-a well characterized panel of 1000 cancer cell lines for anticancer drug development

10.1158/1538-7445.am2019-1918 ◽

2019 ◽

Author(s):

Feng Hao ◽

Changpeng Liu ◽

Hao Peng ◽

Xiaolin Zhou ◽

Yunhui Fu ◽

...

Keyword(s):

Drug Development ◽

Cell Lines ◽

Cancer Cell ◽

Anticancer Drug ◽

Cancer Cell Lines ◽

Anticancer Drug Development

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An effective strategy for development of docetaxel encapsulated gold nanoformulations for treatment of prostate cancer

Scientific Reports ◽

10.1038/s41598-020-80529-1 ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

S. Thambiraj ◽

R. Vijayalakshmi ◽

D. Ravi Shankaran

Keyword(s):

Prostate Cancer ◽

Drug Delivery ◽

Cell Lines ◽

Cancer Cell ◽

Targeted Drug Delivery ◽

Prostate Cancer Cell ◽

Cancer Cell Lines ◽

Simple Method ◽

Prostate Cancer Cell Lines ◽

Targeted Drug

AbstractNanoformulation based drug delivery is one of the most important research areas in the field of nanomedicine, which provides promising alternatives to the limitations of conventional chemotherapy. Nano drug delivery enables improved pharmacokinetic profile, bioavailability and therapeutic efficiency compared to the regular chemotherapeutic drugs. Herein, we have established a simple method for the synthesis of docetaxel (Dtx) encapsulated poly (ethylene glycol) (PEG) functionalized gold nanoparticles (AuNPs) for targeted drug delivery to prostate cancer. AuNPs were synthesized by the citrate ion reduction method followed by functionalization with thiol-PEG-amine (SH-PEG-NH2). SH-PEG-NH2 functionalized AuNPs were conjugated with the targeting vehicle, folic acid (FA). The anticancer drug, Dtx was encapsulated within AuNPs by the non-covalent linkage method. The physicochemical characteristics of the synthesized nanoformulations were extensively characterized by various spectral and microscopic studies. HR-TEM indicates the average size of the AuNPs is 16 nm and the nanoformulations is 18 nm. The encapsulation efficiency of the Dtx is ~ 96% which is confirmed by the elemental mapping analysis. The in vitro drug release profile of Dtx and AuNPs nanoformulations were studied by the dialysis membrane method. The anticancer activity of docetaxel encapsulated AuNPs were evaluated with prostate cancer cell lines (PC3). The drug encapsulated nanoformulations reduced the cell viability to about 40% (40 µM concentration at 24, 48 and 72 h of treatment). The optical microscopy observation reveals that the damage of prostate cancer cells after exposure to Dtx encapsulated AuNPs. The good cytotoxic activity of the present nanoformulation against prostate cancer cell lines enables its application for targeted drug delivery to prostate cancer.

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