Mitochondrial DNA-based genetic diversity of Anopheles nivipes in North East India

2015 ◽  
Vol 27 (6) ◽  
pp. 4236-4239 ◽  
Author(s):  
N. P. Sarma ◽  
S. Singh ◽  
D. K. Sarma ◽  
D. R. Bhattacharyya ◽  
M. C. Kalita ◽  
...  
2012 ◽  
Vol 40 ◽  
pp. 156-161 ◽  
Author(s):  
V.K. Abdul Kareem ◽  
P.E. Rajasekharan ◽  
B.S. Ravish ◽  
S. Mini ◽  
Anuradha Sane ◽  
...  

2018 ◽  
Vol 7 (6) ◽  
pp. 240-247
Author(s):  
Minakshi Bhattacharjee ◽  
Jutika Boro ◽  
Swati Singha ◽  
Hitesh Boro ◽  
Narbu Gengla ◽  
...  

Since chilies contain high quantities of bioactive potential compounds, they are expected to possess large number of pharmacological values like antioxi-dants, antifungal, antibacterial, anti-inflammatory, antiulcer, diuretics activi-ties and is being employed for the treatment of different ailments in the indigenous system of medicine. The current study aimed at comparative evaluation of antioxidant properties, phytochemicals and RAPD assay from extracts of 6 different types of chilli found in the states of Assam and Arunachal Pradesh of North East India. The plant extracts were screened for phyto-chemicals, antioxidant analysis and RAPD profile. Phytochemical analysis shows abundant presence of alkaloids, phenols, flavonoids, saponins etc. The antioxidants activities of all the aqueous extracts were evaluated using the DPPH method. The antioxidant activity (% oxidation inhibition) ranged from as high as 86.56% in Bhut Jolokia (Capsicum assamicum) extracts to as low as 18.77% in Badami Jolokia (Capsicum annum) extract. Chilli samples studied possess potential health benefits by inhibiting many oxidation reactions caused by free radicals. Genetic diversity in 6 chilli genotypes was analyzed by RAPD markers of the fourteen random primers among which 11 were polymorphic. The phylogenetic tree showed common ancestry and the divergence among the chilli species. Based on the current study findings, it may be concluded the RAPD technology can be a useful tool in explaining the genetic diversity among chilli genotypes.


North East India is extremely rich in natural resources and is a biodiversity hotspot. Many plant species, including Citrus are known to have originated from this region. Citus macroptera Mont. is a wild, endangered species which have been found to exist naturally in various parts of this region, including Meghalaya. To access the genetic variability among genotypes and their phylogeny, 30 genotypes of wild C. macroptera Mont. were collected from Garo Hills of Meghalaya. Single primer based DNA markers viz. RAPD, ISSR, DAMD were utilized to ascertain genetic diversity. The percentage polymorphic bands for RAPD, ISSR, DAMD were found to be 97.71%, 94.67% and 100% respectively. ISSR showed the highest values for both RP (7.67) and MI (5.03) highlighting its efficacy in determining genetic variations. A concatenated approach, Single Primer Amplification Reaction (SPAR) was also followed to assess their genetic diversity. Dendogram generated from SPAR data showed that the South-West Garo Hills population is the most recently evolved amongst all others while West Garo Hills collections retain an ancestral position in the evolutionary time-frame. Population genetics parametres such as Gene flow (Nm) and the diversity among populations (GST) were found to be 1.9894 and 0.2009 respectively. Gene flow estimates (Nm>1) suggests appreciable gene flow in the populations. AMOVA data further supported this with high percentage of variations (92%) within populations whereas variations among populations were about 8% only. Shannon’s information index (I) values and Nei’s gene diversity (h) varied between 0.303-0.423and 0.201-0.285 respectively. The use of SPAR method yields a clear and concise picture of the underlying genetic variabilities, and a detailed and comprehensive data analysis will help conceive efficient and sustainable conservation strategies for this important plant.


Author(s):  
Susmita Das ◽  
Pranab Jyoti Das ◽  
Partha Pratim Das ◽  
Banasmita Das ◽  
Dharmeswar Das ◽  
...  

North east India is one of the exotic hotspots for Asian elephant (Elephas maximus) and harbors about half of the global population of the Asian elephant. Here we present the first population genetic study of free-ranging Asian elephants, examining within- and among-population differentiation by analyzing mitochondrial DNA (mtDNA) differentiation across the Brahmaputra valley and elephant reserves of North east India. Surprisingly, the population here had shown lower mtDNA haplotype diversity with little mtDNA differentiation among localities within the Brahmaputra valley. This suggests extensive gene flow in the past, which is compatible with the home ranges of several hundred square kilometers for elephants in this region. Conversely, the Brahmaputra valley population is genetically distinct at a mitochondrial level than other tested elephant populations. These results significantly indicate the importance of population bottlenecks, social organization, and bio-geographic barriers in shaping the distribution of genetic variations among Asian elephant populations in North east India.


2012 ◽  
Vol 11 (1) ◽  
pp. 76 ◽  
Author(s):  
Devojit K Sarma ◽  
Anil Prakash ◽  
Samantha M O'Loughlin ◽  
Dibya R Bhattacharyya ◽  
Pradumnya K Mohapatra ◽  
...  

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