Weak genetic differentiation in cobia, Rachycentron canadum from Indian waters as inferred from mitochondrial DNA ATPase 6 and 8 genes

2015 ◽  
Vol 27 (4) ◽  
pp. 2819-2821 ◽  
Author(s):  
Linu Joy ◽  
C. Mohitha ◽  
P. R. Divya ◽  
A. Gopalakrishnan ◽  
V. S. Basheer ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Rachycentron Canadum ◽  
Indian Waters ◽  
Atpase 6

scholarly journals Genetic Diversities and Historical Dynamics of Native Ethiopian Horse Populations (Equus caballus) Inferred from Mitochondrial DNA Polymorphisms

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 155
Author(s):  
Kefena Effa ◽  
Sonia Rosenbom ◽  
Jianlin Han ◽  
Tadelle Dessie ◽  
Albano Beja-Pereira
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Sequence Divergence ◽  
Dna Polymorphisms ◽  
Base Pairs ◽  
Feral Horses ◽  
Domestic Horses ◽  
D Loop ◽  
Diversity Estimates

Matrilineal genetic diversity and relationship were investigated among eight morphologically identified native Ethiopian horse populations using polymorphisms in 46 mtDNA D-loop sequences (454 base pairs). The horse populations identified were Abyssinian, Bale, Borana, Horro, Kafa, Kundido feral horses, Ogaden and Selale. Mitochondrial DNA D-loop sequences were characterized by 15 variable sites that defined five different haplotypes. All genetic diversity estimates, including Reynolds’ linearized genetic distance, genetic differentiation (FST) and nucleotide sequence divergence (DA), revealed a low genetic differentiation in native Ethiopian horse populations. However, Kundido feral and Borana domestic horses were slightly diverged from the rest of the Ethiopian horse populations. We also tried to shed some light on the matrilineal genetic root of native Ethiopian horses from a network constructed by combining newly generated haplotypes and reference haplotypes deposited in the GenBank for Eurasian type Turkish Anatolian horses that were used as a genetic conduit between Eurasian and African horse populations. Ninety-two haplotypes were generated from the combined Ethio-Eurasian mtDNA D-loop sequences. A network reconstructed from the combined haplotypes using Median-Joining algorithm showed that haplotypes generated from native Ethiopian horses formed separate clusters. The present result encourages further investigation of the genetic origin of native African horses by retrieving additional mtDNA sequences deposited in the GenBank for African and Eurasian type horses.

scholarly journals ANALYSIS OF GENETIC VARIABILITY AT MITOCHONDRIAL DNA LEVEL IN SOME ROUMANIAN PHYTOPHTHORA INFESTANS ACCESSIONS

1970 ◽  
Vol 63 ◽  
Author(s):  
C. Botez ◽  
V. Florian ◽  
I. Oroian ◽  
G. Morar ◽  
Meda Lucaci
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Phytophthora Infestans ◽  
Restriction Enzymes ◽  
Molecular Polymorphism ◽  
Caps Markers

Analysis of secondary molecular polymorphism at the level of amplified mitochondrial DNA, digested by restriction enzymes (CAPS markers), revealed genetic differentiation among ten Phytophthora infestans accessions. Mitochondrial DNA was amplified with four pairs of primers (H1,H2,H3 and H4) and after that the products of amplification were digested with Hha I (for H1 products of amplification), Msp I (for H2 products of amplification) and Eco R I (for H3 and H4 products of amplification) restriction enzymes. On this basis we have established that the ten Phytophthora infestans accessions belongs to two haplotypes, one accession belongs to Ia haplotyp and the others belong to IIa haplotype.

scholarly journals Dynamic genetic diversity and population structure of Coreius guichenoti

ZooKeys ◽  
2021 ◽  
Vol 1055 ◽  
pp. 135-148
Author(s):  
Dongqi Liu ◽  
Feng Lan ◽  
Sicai Xie ◽  
Yi Diao ◽  
Yi Zheng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Population Structure ◽  
Genetic Differentiation ◽  
Yangtze River ◽  
Fish Passage ◽  
The Yangtze River ◽  
Artificial Breeding ◽  
Significant Difference ◽  
High Elevations

To investigate the genetic effects on the population of Coreius guichenoti of dam constructions in the upper reaches of the Yangtze River, we analyzed the genetic diversity and population structure of 12 populations collected in 2009 and 2019 using mitochondrial DNA (mtDNA) control regions. There was no significant difference in genetic diversity between 2009 and 2019 (P > 0.05), but the population structure tended to become stronger. Genetic differentiation (FST) among five populations (LX, BB, YB, SF and JA) collected in 2009 was not significant (P > 0.05). However, some populations collected in 2019 were significantly differentiated (P < 0.05), indicating that the population structure has undergone change. A correlation analysis showed that the genetic diversity of the seven populations collected in 2019 was significantly negatively correlated with geographical height (r = −0.808, P = 0.028), indicating that the populations at high elevations were more vulnerable than those at low elevations. In order to prevent the further decrease of genetic diversity and population resources, some conservation and restoration suggestions, such as fish passage and artificial breeding, are put forward.

Mitochondrial DNA Study Reveals the Cryptic Species Penaeus japonicus (form-II) in Indian Waters

2019 ◽  
Vol 86 (sp1) ◽  
pp. 149
Author(s):  
Tharabenahalli Nagaraju Vinay ◽  
Jani Angel Jesudhas Raymond ◽  
Vinaya Kumar Katneni ◽  
Radhakrishnapillai Aravind ◽  
Changaramkumarath Paran Balasubramanian ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cryptic Species ◽  
Penaeus Japonicus ◽  
Indian Waters

scholarly journals Morphological and genetic differentiation of Bufo toads: two cryptic species in Western Europe (Anura, Bufonidae)

2013 ◽  
Vol 82 (4) ◽  
pp. 147-169 ◽  
Author(s):  
Jan W. Arntzen ◽  
Jacob McAtear ◽  
Ernesto Recuero ◽  
Janine M. Ziermann ◽  
Annemarie Ohler ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Western Europe ◽  
Nuclear Dna ◽  
Sequence Data ◽  
Bufo Bufo ◽  
Sensu Stricto ◽  
Additional Species ◽  
Morphometric Characters ◽  
Evolutionary Context

The Common toad Bufo bufo sensu lato is a widespread, morphologically conserved taxon. Recent studies have uncovered deep genetic differentiation between population groups, highlighting the need to revise the current taxonomy of the group and recognize additional species. Here we investigate patterns of variation in molecular (a mitochondrial DNA restriction enzyme assay and sequence data for two nuclear DNA fragments totalling 979 bp) and 17 morphological variables in Northern France where two of these groups meet (B. bufo sensu stricto and B. spinosus), in order to delineate their contact zone and uncover characters that would allow discrimination of the two taxa. Mitochondrial DNA data show an abrupt transition from areas where B. bufo is present to those inhabited by B. spinosus, with a narrow area of overlap east of the city of Caen. Morphometric characters, particularly those related to the positioning of the parotoid glands and metatarsal tubercle shape and size, proved useful in discriminating between species (AUC ≥ 0.97, kappa ≥ 0.79). We then used the differentiating character states to allocate over 300 museum specimens from Western Europe to either species with consistent results, including comparable values of AUC and kappa of the identification models, indicating that models could successfully be applied across datasets. We summarize available evidence relevant to the delineation of the distribution of B. bufo and B. spinosus in France and discuss the characters differentiating both species in an evolutionary context. In view of the observed morphological and genetic differentiation and the absence of unequivocal evidence for widespread hybridization we support the view that B. bufo and B. spinosus are best considered different species. Finally, we propose that ‘parotoids in parallel position’ and a thin and smooth skin are derived character states for B. bufo over the northern part of its range.

Strong genetic differentiation of the clam Meretrix lamarckii in the China Sea revealed by mitochondrial DNA marker

2019 ◽  
Vol 47 (1) ◽  
pp. 693-702 ◽  
Author(s):  
Jiantong Feng ◽  
Zeqin Fu ◽  
Yahong Guo ◽  
Jiji Li ◽  
Baoying Guo ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Differentiation ◽  
Dna Marker ◽  
China Sea

Evidence of genetic differentiation in cigar wrasse Cheilio inermis (Labridae) within the western Indian Ocean

Genome ◽  
2020 ◽  
Vol 63 (10) ◽  
pp. 493-502
Author(s):  
Sisanda Mayekiso ◽  
Gavin Gouws ◽  
Monica Mwale ◽  
Ofer Gon
Keyword(s):  
Indian Ocean ◽  
Genetic Differentiation ◽  
Larval Dispersal ◽  
Sequence Data ◽  
Allelic Diversity ◽  
Nuclear Gene ◽  
Western Indian Ocean ◽  
Parental Origin ◽  
Pelagic Larvae ◽  
Atpase 6

Patterns of genetic structure and connectivity of the monotypic cigar wrasse Cheilio inermis within western Indian Ocean (WIO) are poorly understood. Whether the species exists as a single panmictic population across the WIO is unclear. Sequence data were generated from two mitochondrial genes (cytochrome b and ATPase 6) and one nuclear intron (S7 intron I). High levels of haplotype and allelic diversity (h = 0.88–0.98; A = 0.95–0.98), along with low nucleotide diversities were observed across all markers. The pairwise ΦST values indicated differentiation of Tanga from the four WIO localities (Inhaca, Nosy Bé, Gazi, and Shimoni), as well as differentiation between the northernmost WIO localities. AMOVAs indicated high differentiation among defined locality groups, whereas nuclear gene analysis found little differentiation among groups. The observed genetic differentiation in C. inermis could be caused by oceanic barriers, and by limited larval dispersal with the pelagic larvae possibly settling near their parental origin and promoting differentiation.

scholarly journals Conservative plumage masks extraordinary phylogenetic diversity in the Grallaria rufula (Rufous Antpitta) complex of the humid Andes

The Auk ◽  
2020 ◽  
Vol 137 (3) ◽  
Author(s):  
R Terry Chesser ◽  
Morton L Isler ◽  
Andrés M Cuervo ◽  
C Daniel Cadena ◽  
Spencer C Galen ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Genetic Differentiation ◽  
Genetic Divergence ◽  
Phylogenetic Diversity ◽  
Biological Species ◽  
Cryptic Speciation ◽  
Species Groups ◽  
River Valleys ◽  
Prime Location

Abstract The Grallaria rufula complex is currently considered to consist of 2 species, G. rufula (Rufous Antpitta) and G. blakei (Chestnut Antpitta). However, it has been suggested that the complex, populations of which occur in humid montane forests from Venezuela to Bolivia, comprises a suite of vocally distinct yet morphologically cryptic species. We sequenced nuclear and mitochondrial DNA for 80 individuals from across the distribution of the complex to determine the extent of genetic variation between and within described taxa. Our results revealed 18 geographically coherent clades separated by substantial genetic divergence: 14 within rufula, 3 within blakei, and 1 corresponding to G. rufocinerea (Bicolored Antpitta), a species with distinctive plumage found to be nested within the complex. Neither G. rufula nor G. blakei as presently defined was monophyletic. Although 6 of the 7 recognized subspecies of G. rufula were monophyletic, several subspecies contained substantial genetic differentiation. Genetic variation was largely partitioned across recognized geographic barriers, especially across deep river valleys in Peru and Colombia. Coalescent modeling identified 17 of the 18 clades as significantly differentiated lineages, whereas analyses of vocalizations delineated 16 biological species within the complex. The G. rufula complex seems unusually diverse even among birds of the humid Andes, a prime location for cryptic speciation; however, the extent to which other dispersal-limited Andean species groups exhibit similar degrees of cryptic differentiation awaits further study.

Oligomycin sensitivity of mitochondrial F1F0-ATPase in diabetes-prone BHE/Cdb rats

1999 ◽  
Vol 277 (4) ◽  
pp. E702-E707 ◽  
Author(s):  
Sook-Bae Kim ◽  
Carolyn D. Berdanier
Keyword(s):  
Mitochondrial Dna ◽  
Oxygen Consumption ◽  
Atpase Activity ◽  
Dna Sequence ◽  
Response Curve ◽  
Human Fibroblasts ◽  
Sprague Dawley ◽  
Isolated Mitochondria ◽  
Sprague Dawley Rats ◽  
Atpase 6

BHE/Cdb and Sprague-Dawley rats differ in their mitochondrial DNA sequence for the ATPase 6 (“subunit a”) gene. Base substitutions in this sequence result in the substitution of asparagine for aspartate at position 101 and the substitution of serine for leucine at position 129. Differences in sensitivity to oligomycin were observed. When the isolated F1F0-ATPase complex was studied and ATPase activity was assessed, that which was isolated from the BHE/Cdb rats was less sensitive to oligomycin inhibition than that which was isolated from the Sprague-Dawley rats. In contrast, when oxygen consumption was measured [oxygen phosphorylation (OXPHOS)] and a dose-response curve was generated with isolated mitochondria from these two strains, there was a shift to the left for the BHE/Cdb rat mitochondria. These mitochondria were more sensitive to oligomycin inhibition of OXPHOS than were mitochondria isolated from Sprague-Dawley rats. The OXPHOS results are consistent with those from human fibroblasts having either a normal or mutated ATPase 6 gene.

Sign in / Sign up

Export Citation Format

Share Document