ESTIMATION OF DEGRADATION POTENTIAL OF THE BACTERIAL DEGRADERS OF CHLORINE DERIVATIVES OF PHENOL OF THE INDUSTRIAL ECOTOPES IN UFA

The ability of eight natural isolates of the two ecotopes to separately use 2,5-dichlorophenol (2,5-DCP) and 2,4,6-trichlorophenol (2,4,6-TCP) as the sole source of carbon and energy were studied. Probably the chlorine substituent at the 5th position concerning the toxicity of the substrate is more important than its total amount because from the eight strains five used 2,4,6-TCP and only one used 2,5-DCP. No one of the studied strains did not show an antagonistic effect on other cultures. This indicates to the possibility of their combined use in technologies for the remediation of the environment from pollutants of a chloroaromatic nature.

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In vitro activity of allicin combined with two antibiotics on intestinal Shigella

Infection International ◽

10.1515/ii-2017-0152 ◽

2017 ◽

Vol 6 (1) ◽

pp. 25-29 ◽

Cited By ~ 1

Author(s):

Yuchi Jia ◽

Xiaomei Wu

Keyword(s):

Intestinal Tract ◽

Inhibitory Concentration ◽

Synergistic Effects ◽

Antagonistic Effect ◽

Additive Effects ◽

In Vitro Activity ◽

Antibacterial Effects ◽

Combined Application ◽

Combined Use

Abstract Objective We aimed to evaluate the combined antibacterial effects of allicin in combination with levofloxacin and ceftriaxone on Shigella isolated from the intestinal tract in vitro. Materials and Methods Using a checkerboard design, broth microdilution assay was used to test the effects of the compounds on the organism. We also determined the MIC of the two groups of antibacterial drugs against 30 strains of Shigella and calculated the fractional inhibitory concentration (FIC) index, to judge the combination effect. Result After the combined application of allicin and ceftriaxone the MIC decreased significantly. Distribution of the FIC index was as follows: FIC ≤0.5, accounting for 10%; 0.5< FIC ≤1.0, accounting for 60%; 1 < FIC ≤2, accounting for 30%; FIC >2, percentage is zero. After combined application of allicin and levofloxacin, distribution of FIC index was as follows: FIC≤0.5, ratio is zero; 0.5< FIC ≤1, accounting for 56.7%; 1 < FIC ≤2, accounting for 43.3%; FIC >2, ratio is zero. Conclusion After the combined use of ceftriaxone, levofloxacin, and allicin, most of the tests showed synergistic effects and additive effects on Shigella, while some of them showed no correlation and no antagonistic effect.

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Effect of Defoliation and Fe and Mn Spraying on Quality and Quantity Characteristics of Sunflower (Helianthus annuus L.)

Notulae Scientia Biologicae ◽

10.15835/nsb336225 ◽

2011 ◽

Vol 3 (3) ◽

pp. 113-119 ◽

Cited By ~ 1

Author(s):

Farshid VAZIN ◽

Mojtaba HASSANZADEHDELOUEI ◽

Mohamadjavad KHERADMAND

Keyword(s):

Seed Yield ◽

Seed Weight ◽

Foliar Application ◽

Antagonistic Effect ◽

Leaf Removal ◽

Negative Effects ◽

Combined Use ◽

Fe And Mn ◽

Defoliation Treatment ◽

Severe Leaf

The study was aimed at identifying the amount of reduction in the negative effects of leaf removal on both quality and quantity of sunflower as a result of foliar application of Fe and Mn. Severe leaf removal reduced the seed yield and seed weight to 11% and 10.5% respectively. The use of Fe in comparison to control increased the seed yield and seed weight up to 12% and 10% respectively. The use of Mn in comparison to control caused an increase in seed number up to 11.6% but caused a reduction in seed weight to 10%. The use of Fe in normal foliar defoliation treatment (40% defoliation) caused a significant increase in seed yield but no significant effect was observed in sever defoliation treatment (80% defoliation). Although using Mn in all treatments led to a rise in seed yield, the effect was not significant, it could be concluded that the combined use of Mn and Fe had no significant influence on sunflower yield, so there might be an antagonistic effect between the two.

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Eugenol and Isoeugenol In Association with Antifungal Against Cryptococcus neoformans

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i4.8133 ◽

2017 ◽

Vol 9 (4) ◽

Author(s):

Pinheiro L. S. ◽

Sousa J. P. ◽

Barreto N. A. ◽

Dantas T B ◽

...

Keyword(s):

Cryptococcus Neoformans ◽

Amphotericin B ◽

Inhibitory Concentration ◽

Synergistic Effects ◽

Antagonistic Effect ◽

Antifungal Drugs ◽

Antifungal Effect ◽

Beneficial Effects ◽

Combined Use ◽

Antifungal Effects

The antifungal therapy combined is used in clinical practice of several mycoses as it may increase the efficacy of the treatment. The use of natural products (phytochemicals) in combination with conventional antifungal drugs has been related to beneficial effects, mainly synergistic effects. The aim of this study was to evaluate the effect of the combined use of eugenol / isoeugenol, compounds with recognized antimicrobial activity, in association with antifungal amphotericin B against strains of Cryptococcus neoformans. The combined antifungal effect were be determined from the Fraction Inhibitory Concentration index - checkerboard technique. The results obtained in this study showed that eugenol in combination with amphotericin B had antagonistic effect against the strains of C. neoformans, LM 615 and INCQS 40221 (FIC index 6.0 and 4.0), respectively. The combination of the isoeugenol and amphotericin B also showed antagonistic effects for both the LM 615 strain and INCQS 40221 (FIC index 6.0 and 5.0), respectively. This study contributed to the understanding of the antifungal effects of the association of phenylpropanoids (eugenol / isoeugenol) with amphotericin B. Further studies are needed to evaluate and compare the effects of the association of these phytochemicals with other conventional antifungal drugs used against C. neoformans.

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Derivatives of acridone acetic acid in pediatric practice

Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics) ◽

10.21508/1027-4065-2019-64-2-110-116 ◽

2019 ◽

Vol 64 (2) ◽

pp. 110-116

Author(s):

A. A. Shuldyakov ◽

E. P. Lyapina ◽

V. K. Polyakov ◽

Yu. B. Barylnik ◽

O. B. Lisko ◽

...

Keyword(s):

Acetic Acid ◽

Viral Infections ◽

Allergic Diseases ◽

Pediatric Practice ◽

Tick Borne Encephalitis ◽

Combined Use ◽

Respiratory Viral Infections ◽

Sick Children ◽

Derivatives Of ◽

Rotavirus Infections

The article justifies the use of interferon inducers in a clinical practice of pediatrician. The authors isolated a group of derivatives of acridone acetic acid, which effectiveness is associated with the features of pharmacokinetics and pharmacological activity. They demonstrated preventive and therapeutic efficacy of cycloferon in the complex treatment of children with influenza and other acute respiratory viral infections, herpes virus, arbovirus, rotavirus infections, viral hepatitis and tick-borne encephalitis due to its immunomodulatory potency. The article analyzed the use of cycloferon in frequently sick children, patients with allergic diseases, chronic tonsillitis, etc.; also it showed a decrease in the frequency of relapses of both infectious and allergic diseases, the possibility of combined use with other etiotropic and symptomatic drugs.

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Antitumor Effects of MRS5698, a Cordycepin Derivative, on Endometrial Cancer Cells

Natural Product Communications ◽

10.1177/1934578x19881564 ◽

2019 ◽

Vol 14 (10) ◽

pp. 1934578X1988156

Author(s):

Pedro Fong ◽

Cheng-I Loi ◽

Wan-Fong U ◽

Chou-I Choi ◽

Tao Yi ◽

...

Keyword(s):

Endometrial Cancer ◽

Inhibitory Effect ◽

Antagonistic Effect ◽

Cordyceps Sinensis ◽

New Drugs ◽

Cancer Drug ◽

Antitumor Effects ◽

Combined Use ◽

Ishikawa Cells

Endometrial cancer drug treatments often produce undesirable effects. Thus, discovering new drugs with fewer side effects is required. Cordycepin is a constituent of Cordyceps sinensis, which has been proven to inhibit tumor growth by stimulating the adenosine A3 receptor (A3R). However, cordycepin is rapidly degraded by adenosine deaminase (ADA) and has a clinically unacceptable short half-life. One of its derivatives, MRS5698, was predicted to exhibit antitumor effects with a poor affinity to ADA by our previous validated in silico experiments. The purpose of this study was to explore the possibilities of using MRS5698 as a novel antitumor agent through experiments on Ishikawa and HEC-1A cells. The detection of inhibition and apoptotic rate of MRS5698 and cisplatin, and their combination, on Ishikawa and HEC-1A cells were performed by MTT assays and flow cytometry, respectively. The inhibition rates of MRS5698 on Ishikawa and HEC-1A cells were both significantly higher than the control groups ( P < 0.05). MRS5698 produced a higher inhibitory effect on HEC-1A cells than on Ishikawa cells with IC50 values of 20.55 and 27.25 μg/mL, respectively. MRS5698 had a stronger inhibitory effect than cisplatin on HEC-1A cells. The Annexin V-FITC/propidium iodide assays demonstrated that the total rate of apoptosis of MRS5698 on HEC-1A cells was higher than that on Ishikawa cells. The results of MTT assay and cellular apoptosis showed that the combined use of MRS5698 and cisplatin produces dose-independent antagonistic effects. MRS5698 produced antitumor effects on both cell lines, which were better than that of cordycepin. However, the combined use of MRS5698 and cisplatin produced an antagonistic effect. A further in vivo study could be considered for investigating the antitumor effects of either MRS5698 monotherapy or MRS5698 in combination with other nonplatinum-based chemotherapeutic drugs in treating endometrial cancer.

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Influence of chlorine and methyl substituents and their position on the antimicrobial activities and crystal structures of 4-methyl-1,6-diphenylpyrimidine-2(1H)-selenone derivatives

Acta Crystallographica Section C Structural Chemistry ◽

10.1107/s205322962100975x ◽

2021 ◽

Vol 77 (10) ◽

Author(s):

Izabela Korona-Głowniak ◽

Wojciech Nitek ◽

Waldemar Tejchman ◽

Ewa Żesławska

Keyword(s):

Crystal Structure ◽

Biological Activity ◽

Intermolecular Interactions ◽

Current Knowledge ◽

Antimicrobial Activities ◽

Mutual Orientation ◽

Antibacterial And Antifungal Activities ◽

Chlorine Substituent ◽

Antibacterial And Antifungal ◽

Derivatives Of

Derivatives of 4-methyl-1,6-diphenylpyrimidine-2(1H)-selenone show very strong antimicrobial activity. In order to extend the current knowledge about the features responsible for the biological activity, crystal structure analyses are presented for 4-methyl-1-(2-methylphenyl)-6-phenylpyrimidine-2(1H)-selenone (1), 4-methyl-1-(3-methylphenyl)-6-phenylpyrimidine-2(1H)-selenone (2), 4-methyl-1-(4-methylphenyl)-6-phenylpyrimidine-2(1H)-selenone (3) (all C18H16N2Se) and 1-(4-chlorophenyl)-4-methyl-6-phenylpyrimidine-2(1H)-selenone (4) (C17H13ClN2Se). Furthermore, the antibacterial and antifungal activities of these compounds were evaluated. All the presented derivatives crystallize in the space group P21/c with one molecule in the asymmetric unit. The molecular geometries differ slightly in the mutual orientation of the rings. The packing of molecules in the crystals is dominated by C—H...N and C—H...Se intermolecular interactions. Additionally, in the crystal structure of 4, C—H...Cl intermolecular interactions are observed. The introduction of a methyl or chlorine substituent improves the biological activity, while its position significantly affects biological activity only in case of the chlorine substituent.

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EFFECTS OF COMBINED USE OF DERIVATIVES OF 4-ACETYLAMINO-NAPHTALENE-1-LAUROULSULPHONAMID AND X-RAY

The KITAKANTO Medical Journal ◽

10.2974/kmj1951.10.6_730 ◽

1960 ◽

Vol 10 (6) ◽

pp. 730-747

Author(s):

Atsuhiro Tsuchiya

Keyword(s):

X Ray ◽

Combined Use ◽

Derivatives Of

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Synergistic promoting effect of ball milling and Fe(ii) catalysis for cross-dehydrogenative-coupling of 1,4-benzoxazinones with indoles

Heterocyclic Communications ◽

10.1515/hc-2020-0123 ◽

2021 ◽

Vol 27 (1) ◽

pp. 57-62

Author(s):

Ali Sharifi ◽

Zahra Babaalian ◽

M. Saeed Abaee ◽

Maryam Moazami ◽

Mojtaba Mirzaei

Keyword(s):

Ball Milling ◽

Room Temperature ◽

Coupling Method ◽

Promoting Effect ◽

Mild Conditions ◽

Dehydrogenative Coupling ◽

Time Period ◽

Combined Use ◽

Derivatives Of

Abstract In this work, a novel C(sp3)–C(sp2) cross-dehydrogenative-coupling method is developed to react benzoxazin-2-one derivatives with various indoles. As a result, combined use of ball milling and Fe(ii) catalysis leads to rapid coupling of 1,4-benzoxazinones with derivatives of indole. Under the conditions, derivatives of 1 couple with various indoles at room temperature to produce good yields of the desired compounds within 0.5–2 h time period. Thus, derivatives of both starting materials couple smoothly under relatively mild conditions to give good yields of 3.

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Cytoprotective effect of silybin against lasalocid-induced toxicity in HepG2 cells

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0038 ◽

2013 ◽

Vol 16 (2) ◽

pp. 275-282 ◽

Cited By ~ 6

Author(s):

L. Radko ◽

W. Cybulski ◽

W. Rzeski

Keyword(s):

Hepg2 Cells ◽

Combination Index ◽

Antagonistic Effect ◽

Cytoprotective Effect ◽

Cytotoxic Effects ◽

Mtt Test ◽

Main Compound ◽

Combined Use ◽

Edible Tissues ◽

Pharmacologically Active

AbstractLasalocid is an ionophore coccidiostatic agent frequently used in poultry. Its extensive use causes the formation of residues in edible tissues and eggs which may pose a risk to consumers. Silybin is the main compound extracted from the herb milk thistle Silybum marianum and its hepatoprotective effect has been reported in literature.The aim of the study was to compare lasalocid and silybin cytotoxic effects followed by their combined use in HepG2 cell line. A cytoprotective effect resulting from the interaction of both pharmacologically active substances was measured.In this study, an MTT test, coomassie brillant blue binding test, and LDH release test determined the effective concentration (EC50) of the compounds. The isobolograms and combination index were used to assess the nature of interaction.The lowest EC50-value for lasalocid was established via the MTT test. This study revealed a lack of silybin cytotoxic effect on the cells. Co-actions of the two drugs led to a significant decrease of lasalocid cytotoxicity. The isobolograms and combination index showed a remarkable antagonistic effect in the course of silybin and lasalocid interaction.The results indicate that silybin revealed a cytoprotective effect when incubated with lasalocid since its cytotoxic impact on HepG2 cells has been significantly diminished.

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