scholarly journals ISOLATION AND MOLECULAR IDENTIFICATION OF FRESHWATER MICROALGAE IN MANINJAU LAKE WEST SUMATERA

2018 ◽  
Author(s):  
zulkarnain chaidir ◽  
Neri Fadjria ◽  
Armaini ◽  
Rahadian Zainul
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
18S Rdna ◽  
Gene Bank ◽  
Freshwater Microalgae ◽  
Eukaryotic Microorganisms ◽  
West Sumatra ◽  
Percent Similarity

Microalgae are photosynthetic prokaryotic and eukaryotic microorganisms. In this research, isolation of microalgae from Maninjau lake West Sumatra was identified by morphological and molecular identification by PCR using primers for 16S rDNA and 18S rDNA prokaryotic and eukaryotic microalgae. The results of this study were obtained 3 isolates of microalgae that can be isolated from Maninjau lake West Sumatra are Scenedesmus (code isolate AUMA-020) with a percent similarity of 95%, Uncultured cyanobacterium (code isolates AUMA-023) with a percent similarity of 80% and Limnothrix (code isolates AUMA-019) has a percent similarity of 98% based on data gene Bank blast results.

scholarly journals Cytological And Molecular Identification Of Haemogregarina Stepanowi In Blood Samples Of The European Pond Turtle (Emys Orbicularis) From Quarantine At Belgrade Zoo

2015 ◽  
Vol 65 (4) ◽  
pp. 443-453 ◽  
Author(s):  
Jòzsef Özvegy ◽  
Darko Marinković ◽  
Miloš Vučićević ◽  
Bojan Gajić ◽  
Jevrosima Stevanović ◽  
...  
Keyword(s):  
Molecular Identification ◽  
18S Rdna ◽  
Clinical Symptoms ◽  
Health Condition ◽  
Rdna Sequence ◽  
Future Research ◽  
Emys Orbicularis ◽  
Direct Dna Sequencing ◽  
Hematological Analysis ◽  
18S Rdna Sequence

Abstract Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.

scholarly journals Improved molecular identification of Thermoactinomyces spp. associated with mushroom worker's lung by 16S rDNA sequence typing

2002 ◽  
Vol 51 (12) ◽  
pp. 1117-1127 ◽  
Author(s):  
JIRU XU ◽  
JAMES EVANS ◽  
J. STUART ELBORN ◽  
JOHN E. MOORE ◽  
JOHN G. BARR ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Rdna Sequence ◽  
16S Rdna Sequence

scholarly journals Identifikasi Molekuler Bakteri Endofit Penghasil L-asparaginase yang Diisolasi dari Mangrove Buta-Buta (Excoecaria agallocha)

2019 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Asep Awaludin Prihanto ◽  
Randy Fahrudin Ardiansyah ◽  
Ken Audia Pradarameswari
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Endophytic Bacteria ◽  
Sequence Data ◽  
Enterobacter Cloacae ◽  
Selective Medium ◽  
Mangrove Plant ◽  
Excoecaria Agallocha ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.

scholarly journals High-throughput sequencing revealed differences of microbial community structure and diversity between healthy and diseased Caulerpa lentillifera

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Zhourui Liang ◽  
Fuli Liu ◽  
Wenjun Wang ◽  
Pengyan Zhang ◽  
Xiutao Sun ◽  
...  
Keyword(s):  
Information Processing ◽  
Community Structure ◽  
Microbial Community ◽  
16S Rdna ◽  
High Throughput ◽  
Microbial Community Structure ◽  
18S Rdna ◽  
High Throughput Sequencing ◽  
Rdna Sequencing ◽  
Caulerpa Lentillifera

Abstract Background Caulerpa lentillifera is one of the most important economic green macroalgae in the world. Increasing demand for consumption has led to the commercial cultivation of C. lentillifera in Japan and Vietnam in recent decades. Concomitant with the increase of C. lentillifera cultivation is a rise in disease. We hypothesise that epiphytes or other microorganisms outbreak at the C. lentillifera farm may be an important factor contributing to disease in C. lentillifera. The main aims are obtaining differences in the microbial community structure and diversity between healthy and diseased C. lentillifera and key epiphytes and other microorganisms affecting the differences through the results of high-throughput sequencing and bioinformatics analysis in the present study. Results A total of 14,050, 2479, and 941 operational taxonomic units (OTUs) were obtained from all samples using 16S rDNA, 18S rDNA, and internal transcribed spacer (ITS) high-throughput sequencing, respectively. 16S rDNA sequencing and 18S rDNA sequencing showed that microbial community diversity was higher in diseased C. lentillifera than in healthy C. lentillifera. Both PCoA results and UPGMA results indicated that the healthy and diseased algae samples have characteristically different microbial communities. The predominant prokaryotic phyla were Proteobacteria, Planctomycetes, Bacteroidetes, Cyanobacteria, Acidobacteria, Acidobacteria and Parcubacteria in all sequences. Chlorophyta was the most abundant eukaryotic phylum followed by Bacillariophyta based on 18S rDNA sequencing. Ascomycota was the dominant fungal phylum detected in healthy C. lentillifera based on ITS sequencing, whereas fungi was rare in diseased C. lentillifera, suggesting that Ascomycota was probably fungal endosymbiont in healthy C. lentillifera. There was a significantly higher abundance of Bacteroidetes, Cyanobacteria, Bacillariophyta, Ulvales and Tetraselmis in diseased C. lentillifera than in healthy C. lentillifera. Disease outbreaks significantly change carbohydrate metabolism, environmental information processing and genetic information processing of prokaryotic communities in C. lentillifera through predicted functional analyses using the Tax4Fun tool. Conclusions Bacteroidetes, Cyanobacteria, Bacillariophyta, Ulvales and Tetraselmis outbreak at the C. lentillifera farm sites was an important factor contributing to disease in C. lentillifera.

Molecular identification of the first human isolate belonging to the Veillonella ratti–Veillonella criceti group based on 16S rDNA and dnaK gene sequencing

2005 ◽  
Vol 156 (4) ◽  
pp. 603-607 ◽  
Author(s):  
Hélène Marchandin ◽  
Corinne Teyssier ◽  
Estelle Jumas-Bilak ◽  
Maxime Robert ◽  
Anne-Catherine Artigues ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Gene Sequencing ◽  
Human Isolate

Molecular phylogeny of the mud lobsters and mud shrimps (Crustacea : Decapoda : Thalassinidea) using nuclear 18S rDNA and mitochondrial 16S rDNA

2002 ◽  
Vol 16 (6) ◽  
pp. 839 ◽  
Author(s):  
C. C. Tudge ◽  
C. W. Cunningham
Keyword(s):  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
16S Rdna ◽  
18S Rdna ◽  
Strong Support ◽  
Ribosomal Genes ◽  
Bayesian Analyses ◽  
The Family ◽  
Mitochondrial 16S Rdna

Partial sequences of the 18S nuclear and 16S mitochondrial ribosomal genes were obtained for 14�species of thalassinidean shrimp (families Callianassidae, Laomediidae, Strahlaxiidae, Thalassinidae and Upogebiidae) and a further six species in related decapod infraorders (families Aeglidae, Astacidae, Lithodidae, Palinuridae, Raninidae and Scyllaridae). Maximum-likelihood and Bayesian analyses show equivocal support for the monophyly of the Thalassinidea, but show strong support for division of the infraorder into two major clades. This dichotomy separates representatives in the Upogebiidae, Laomediidae and Thalassinidae from those in the Strahlaxiidae and Callianassidae. The Laomediidae is shown to be paraphyletic, with the thalassinid species, Thalassina squamifera, being placed on a branch between Axianassa and a clade comprising Jaxea and Laomedia, the three current laomediid genera. For a monophyletic Laomediidae, the family Axianassidae should be resurrected for the genus Axianassa.

scholarly journals Molecular detection of Rickettsia genus in chigger mites (Trombidiformes: Trombiculidae) collected on small mammals in southeastern brazilian

2019 ◽  
Vol 28 (4) ◽  
pp. 563-568
Author(s):  
Fernando de Castro Jacinavicius ◽  
Ricardo Bassini-Silva ◽  
Sebastián Muñoz-Leal ◽  
Cal Welbourn ◽  
Ronald Ochoa ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Small Mammals ◽  
18S Rdna ◽  
Molecular Detection ◽  
Molecular Analyses ◽  
First Report ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
First Time

Abstract Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to “Candidatus Rickettsia colombianensi”, a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.

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